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The Multilayer’s Control of ARGONAUTE 1 Contents

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Molecular Plant 2020年第1期13卷 1-3页

作者： Jean Molinier institut de biologie moleculaire des plantes 12 Rue du General Zimmer67000 StrasbourgFrance

ARGONAUTE proteins(AGO)are key effectors of the RNA silencing pathways involved in the regulation of gene expression and in antiviral defense(Vaucheret,2008).Among the 10 AGO proteins present in Arabidopsis,AG01 is essential for plant development and defense response,participating in both microRNA(miRNA)-and small interfering RNA(siRNA)-mediated gene silencing(Vaucheret,2008).Given that AG01 depletion is lethal,only hypomorphic mutant plants are viable and display a large variety of developmental phenotypes(Vaucheret,2008).Importantly,the fine tuning of AG01 content is an important process orchestrated by several different pathways(Cho et al.,2016).It was recently described that the mode of action of AG01 is not only restricted to the posttranscriptional gene silencing pathway taking place in the ***,several nuclear AG01 functions have been identified(Bajczyk et al.,2019).Thus,the characterization of the different AG01 pools implies that specific subcellular pathways might trigger AGOI turnover and that complex regulatory processes might interplay to specifically fine tune AG01 content.

关键词： viable depletion restricted

Dynamic Regulation of deLLA Protein Activity： SPINDLY and SECRET AGENT Unmasked!

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Molecular Plant 2017年第6期10卷 785-787页

作者： Lucie Camut Jean-Michel Daviere Patrick Achard institut de biologie moleculaire des plantes CNRS Universite de Strasbourg 12 rue du General Zimmer 67084 Strasbourg Cedex France

Plants are sessile organisms that integrate growth responses to multiple endogenous and environmental signals. The deLLA family of transcription regulators, first identified as key repressors of gibberellin （GA） signaling, have emerged as a central node of integration, thus providing a mechanism for environmentally responsive growth regulation （Oh et al., 2014）.

关键词： Dynamic Regulation deLLA Protein Activity SECRET AGENT Unmasked

Histone Acetyltransferase AtGCN5/HAG1 Is a Versatile Regulator of developmental and Inducible Gene Expression in Arabidopsis

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Molecular Plant 2010年第4期3卷 670-677页

作者： Caroline Servet Natalia Conde e Silva Dao-Xiu Zhou institut de biologie des plantes Universite Paris-sud 11 91405 Orsay France

Histone acetylation/deacetylation is a dynamic process and plays an important role in gene regulation. Histone acetylation homeostasis is regulated by antagonist actions of histone acetyltransferases （HAT） and deacetylases （HDAC）. Plant genome encodes multiple HATs and HDACs. The Arabidopsis HAT gene AtGCNS/HAGlplays an essential role in many plant development processes, such as meristem function, cell differentiation, leaf and floral organogenesis, and responses to environmental conditions such as light and cold, indicating an important role of this HAT in the regulation of both long-term developmental switches and short-term inducible gene expression. AtGCN5 targets to a large number of promoters and is required for acetylation of several histone H3 lysine residues. Recruitment of AtGCN5 to target promoters is likely to be mediated by direct or indirect interaction with DNA-binding transcription factors and/or by interaction with acetylated histone lysine residues on the targets. Interplay between AtGCN5 and other HATand HDAC is demonstrated to control specific regulatory pathways. Analysis of the role of AtGCN5 in light-inducible gene expression suggests a function of AtGCN5 in preparing chromatin commitment for priming inducible gene activation in plants.

关键词： Light regulation chromatin structure and remodeling gene regulation leaf/vegetative development meristem development.

A Pivotal Role of deLLAs in Regulating Multiple Hormone Signals

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Molecular Plant 2016年第1期9卷 10-20页

作者： Jean-Michel Daviere Patrick Achard institut de biologie moleculaire des plantes (IBMP) UPR2357 associe avec l'Universite de Strasbourg 12 rue General Zimmer 67084 Strasbourg Cedex France

Plant phenotypic plasticity is controlled by diverse hormone pathways, which integrate and convey infor- mation from multiple developmental and environmental signals. Moreover, in plants many processes such as growth, development, and defense are regulated in similar ways by multiple hormones. Among them, gibberellins （GAs） are phytohormones with pleiotropic actions, regulating various growth processes throughout the plant life cycle. Previous work has revealed extensive interplay between GAs and other hor- mones, but the molecular mechanism became apparent only recently. Molecular and physiological studies have demonstrated that deLLA proteins, considered as master negative regulators of GA signaling, inte- grate multiple hormone signaling pathways through physical interactions with transcription factors or reg- ulatory proteins from different families. In this review, we summarize the latest progress in GA signaling and its direct crosstalk with the main phytohormone signaling, emphasizing the multifaceted role of deLLA proteins with key components of major hormone signaling pathways.

关键词： gibberellins deLLAs hormone crosstalk plant development phenotypic plasticity

Jasmonic Acid Oxidase 2 Hydroxylates Jasmonic Acid and Represses Basal defense and Resistance Responses against Botrytis cinerea Infection

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Molecular Plant 2017年第9期10卷 1159-1173页

作者： Ekaterina Smirnova institut de biologie Moléculaire des plantes CNRS Université de Strasbourg Strasbourg France Laboratoire de Chimie Organique Synthétique Institut de Chimie Université de Strasbourg CNRS Strasbourg France

Jasmonates （JAs） orchestrate immune responses upon wound/herbivore injury or infection by necrotro- phic pathogens. Elucidation of catabolic routes has revealed new complexity in jasmonate metabolism. Two integrated pathways attenuate signaling by turning over the active hormone jasmonoyl-isoleucine （JA-Ile） through w-oxidation or deconjugation, and define an indirect route forming the derivative 12OH-JA. Here, we provide evidence for a second 12OH-JA formation pathway by direct jasmonic acid （JA） oxidation. Three jasmonic acid oxidases （JAOs） of the 2-oxoglutarate dioxygenase family catalyze spe- cific oxidation of JA to 12OH-JA, and their genes are induced by wounding or infection by the fungus Botrytis cinerea. JA02 exhibits the highest basal expression, and its deficiency in jao2 mutants strongly enhanced antifungal resistance. The resistance phenotype resulted from constitutive expression of antimi- crobial markers rather than from their higher induction in infected jao2 plants and could be reversed by ectopic expression of any of the three JAOs injao2. Elevated defense injao2 was dependent on the activity of JASMONATE RESPONSE 1 （JAR1） and CORONATINE-INSENSITIVE 1 （COI1） but was not correlated with erihanced JA-Ile accumulation. Instead, jao2 mutant lines displayed altered accumulation of several JA species in healthy and challenged plants, suggesting elevated metabolic flux through JA-Ile. Collectively, these data identify the missing enzymes hydroxylating JA and uncover an important metabolic diversion mechanism for repressing basal JA defense responses.

关键词： 2-oxoglutarate oxygenase jasmonic acid defense regulation hormone metabolism Botrytis

Monothiol Glutaredoxin-BolA Interactions： Redox Control of Arabidopsis thaliana BolA2 and SufE1

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Molecular Plant 2014年第1期7卷 187-205页

作者： Jeremy Couturier Hui-Chen Wu Tiphaine Dhalleine Henri Pegeot Damien Sudre Jose M. Gualberto Jean-Pierre Jacquot Frederic Gaymard Florence Vignols Nicolas Rouhier Universite de Lorraine Interactions Arbres-Microorganismes UMR1136 F-54500 Vandoeuvre-les-Nancy France INRA Interactions Arbres-Microorganismes UMR1136 F-54280 Champenoux France CNRS & UMR186 Resistance des plantes aux Bio-agresseurs Institut de Recherche pour le Developpement BP 64501 34394 Montpellier cedex 5 France Biochimie et Physiologie moleculaire des plantes CNRS/INRA/Universite Montpellier 2 SupAgro. Bat 7 2 place Viala 34060 Montpellier cedex 1 France institut de biologie moleculaire des plantes CNRS UPR2357 F-67084 Strasbourg France

A functional relationship between monothiol glutaredoxins and BolAs has been unraveled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of green fluo- rescent protein （GFP） fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in Arabidopsis thaliana, BolA1 being plastidial, BolA2 nucleo-cytoplasmic, and BolA4 dual-targeted to mitochondria and plastids. Binary yeast two-hybrid experiments demonstrated that all BolAs and SufE 1, via its BolA domain, can interact with all monothiol glutaredoxins. Most interactions between protein couples of the same subcellular compartment have been confirmed by bimolecular fluorescence complementation. In vitro experiments indicated that monothiol glutaredoxins could regulate the redox state of BolA2 and SufE1, both proteins possessing a single conserved reactive cysteine. Indeed, a glutathionylated form of SufE1 lost its capacity to activate the cysteine desuifurase, Nfs2, but it is reactivated by plastidial glutaredoxins. Besides, a monomeric glutathionyiated form and a dimeric disulfide-bridged form of BolA2 can be preferentially reduced by the nucleo-cytoplasmic GrxS17. These results indicate that the glutaredoxin-BolA interaction occurs in several subcellular compartments and suggest that a redox regulation mechanism, disconnected from their capacity to form iron-sulfur cluster-bridged heterodimers, may be physiologically relevant for BolA2 and SufE1.

关键词： BolA glutaredoxin interaction plants redox control.

SDG714 Regulates Specific Gene Expression and Consequently Affects Plant Growth via H3K9 Dimethylation

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Journal of Integrative Plant Biology 2010年第4期52卷 420-430页

作者： Bo Ding Yan Zhu Zhong-Yuan Bu Wen-Hui Shen Yu Yu Ai-Wu Dong State KeyLaboratory of Genetic Engineering Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China institut de biologie moleculaire des plantes du CNRS Universite Louis Pasteur de Strasbourg 67084 Strasbourg Cedex France B. Ding and Y. Zhu contributed equally to this paper

Histone lysine methylation is known to be involved in the epigenetic regulation of gene expression in all eukaryotes including plants. Here we show that the rice SDG714 is primarily responsible for dimethylation but not trimethylaUon on histone H3K9 in vivo. Overexpression of YFP-SDG714 in Arabidopsis significantly inhibits plant growth and this inhibition is associated with an enhanced level of H3K9 dimethylation. Our microarray results show that many genes essential for the plant growth and development were downregulated in transgenic Arabidopsis plants overexpressing YFP-SDG714. By chromatin immunoprecipitation analysis, we show that YFP-SDG714 is targeted to specific chromatin regions and dimethylate the H3Kg, which is linked with heterochromatinization and the downregulation of genes. Most interestingly, when YFP-SDG714 production is stopped, the inhibited plants can partially restore their growth, suggesting that the perturbation of gene expression caused by YFP-SDG714 is revertible. Taken together, our results point to an important role of SDG714 in H3K9 dimethylation, suppression of gene expression and plant growth, and provide a potential method to regulate gene expression and plant development by an on-off switch of SDG714 expression.

关键词： Specific Gene Expression Consequently Affects

Epigenomic Modification and Epigenetic Regulation in Rice

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Journal of Genetics and Genomics 2012年第7期39卷 307-315页

作者： Yu Zhao Dao-Xiu Zhou National Key Laboratory for Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China institut de biologie des plantes Universite' Paris sud Orsay 91405 France

Epigenomes including genome-wide histone modification and DNA methylation profiles are important for genome activity and fof defining gene expression patterns of plant development and responses to various environmental conditions. Rice is the most important crop plant and serves as a model for cereal genomics. Rice epigenomic landscape is emerging and the function of chromatin modification regulators in gene expression, transposon repression and plant development is being characterized. Epigenomic variation that gives rise to stable or transgenerational heritable epialleles related to variation of important agronomical traits or stress responses is being characterized in rice. Implication of epigenomic variation in rice heterosis is being exploited.

关键词： Epigenetics Epigenomics Ot3,za sativa

Chromatin Remodeling in Stem Cell Maintenance in Arabidopsis thaliana

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Molecular Plant 2009年第4期2卷 600-609页

作者： Wen-Hui Shen Lin Xu institut de biologie moleculaire des plantes (IBMP) du CNRS Universite de Strasbourg (UdS) 12 rue du General Zimmer 67084 Strasbourg Cedex France Present address: National Key Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai 200032 China

Pluripotent stem cells are able to both self-renew and generate undifferentiated cells for the formation of new tissues and organs. In higher plants, stem cells found in the shoot apical meristem （SAM） and the root apical meristem （RAM） are origins of organogenesis occurring post-embryonically. It is important to understand how the regulation of stem cell fate is coordinated to enable the meristem to constantly generate different types of lateral organs. Much knowledge has accumulated on specific transcription factors controlling SAM and RAM activity. Here, we review recent evidences for a role of chromatin remodeling in the maintenance of stable expression states of transcription factor genes and the control of stem cell activity in Arabidopsis.

关键词： chromatin structure and remodeling epigenetics meristem development histone chaperone histone modification.