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QUALITY CONtrOL ON THE AUSTENITIC-BAINITIC DUCTILE IRON GEAR

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Journal of Wuhan University of Technology(Materials Science) 2000年第1期15卷 43-48页

作者：朱起凡 harmacy.ankara.edu.tr

By reasonable casting, spheroidizing, in- oculating and heattreating processes to control cupola met- allurgy and the lipuid ironchemical compositions, a new kind of austenitic-bainitic ductile ironwhich can substitute for 20CrMnTi carburizing steel is studied. Thegears made by the cost iron are high in quality, low in weight andthe production cost is greatly reduced. The mechnical proper- ties ofthe gear, including wear resistance, fatigue resis- tance, durabilityand others, can be greatly improved.

关键词： austenitic-bainitic iron gear

Temperature dependent expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis

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Cell Research 2000年第4期10卷 289-302页

作者： KONG WEI HUA ZHENG GU JI NING LU JIA KE TSO Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China College of Life Science, Shandong University, Jinan 250100, China harmacy.ankara.edu.tr

p34cdc2 and Cyclin B1 are key components of cell cycle controlling machine and are believed to play a fundamental role in gametogenesis. It is also well known that, in scrotal mammals, spermatogenesis depends greatly on the maintenance of comparatively low temperature in the scrotum. To investigate whether the expression of cdc2 and Cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and Cyclin B1 in normal and cryptorchid testis. Results showed that the abdominal temperature had no significant influence on the transcription of cdc2 and Cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes, but it blocked the translation of them. Due to the deficiency of p34cdc2 and Cyclin B1, the spermatogonia and pachytene/diplotene primary spermatocytes were unable to form MPF, hence, they couldn't undergo karyokinesis. The development of primary spermatocytes was arrested at the G2 to M phase transition. We also found that testosterone could regulate the Cyclin B1 expression in spermatogenic cells. Muscular injection of testosterone could recover spermatogenesis in the unilateral scrotal testis which was influenced by the contralateral cryptorchid testis, but it could not salvage the spermatogenesis block in the cryptorchid testis.

关键词： Temperature spermatogenesis cdc2 cyclin B8

Isolation and characteristics of autoreactive T cells specific to aggrecan G1 domain from rheumatoid arthritis patients

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Cell Research 2000年第1期10卷 39-49页

作者： LI NING LI DONG QING ZHANG KUANG YAN ZHOU ANNIE CARTMAN JEAN YVES LEROUX A ROBIN POOLE YI PING ZHANG ( Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025, China)(Joint Diseases Laboratory, Shriners Hospital, Dep harmacy.ankara.edu.tr

Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the GI globular domain of the aggrecan (GI) contained the arthritogenic region. To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients, we analyzed the frequency of human G1-specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1-reactive T cell lines from these rheumatoid arthritis patients. The results showed that the G1-specific T cells in PBL were detectable at the range of 4.97 ±0.5 ×10-6 in peripheral blood lymphocytes. We have also generated 15 G1-specific T lymphocyte lines from these pateints with a standard split-well method. All these cells expressed fine specificity to human recombinant G1, but not to unrelated antigen. All the 15 lines expressed a panT cell marker and 13 of them selectively used the αβ T cell receptor. Two of them used rye T cell receptor. The 13 of these T cell lines was CD4 positive. One line expressed CD8. One line expressed both CD4 and CD8. Moreover, 14 out of 15 lines expressed the Th-1 cytokine profile, characterized by interferon-γpositivity and IL-4 negativity. No Th-2 type cell line was generated. These data provide strong evidence in favor of the presence of autoreactive T cells in the rheumatoid arthritis pateints. What is the mechanism(s) that these autoreactive T cells attack self-target and whether these G1-specific, Th-1 type T cell lines can induce arthritis in immune deficiency mice are currently under investigation.

关键词： rG1-specific T cell lines T cell receptor cytokine profile rheumatoid arthritis.

MODIFICATION OF FLAKE REINFORCED FRICTION BRAKE COMPOSITE MATERIAL

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Journal of Wuhan University of Technology(Materials Science) 2000年第1期15卷 54-59页

作者：曹献坤 harmacy.ankara.edu.tr

According to the recent development trend and need of the frictionbrake material, the flake rein- forced friction brake material hasbeen made out by adjust- ing the recipe and techniques. Thetwo-dimensional flake vermiculite is selected as the reinforcedstuffing of the ma- terial; the modified resin is used as the basalbed of the material. The tests manifest that the properties ofmechan- ics are high, the friction coefficients are suitable andstabel, and especially in high temperatures the wear is low. It is anexcellent friction brake material.

关键词： flake reinforced composite material fricition material

transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity

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Cell Research 2000年第4期10卷 303-310页

作者： MA YING HUA JIA HUA HU XIAO GANG ZHOU RUO WANG ZENG ZHEN TONG MEI JIAN FEI LI HE GUO Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of harmacy.ankara.edu.tr

transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.

关键词： γ-aminobutyric acid transporter subtype Ⅰ transgenic mouse obesit€

Genetic aberration in primary hepatocellular carcinoma:correlation between p53 gene mutation and loss-of-heterozygosity on chromosome 16q21-q23 and 9p21-p23

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Cell Research 2000年第4期10卷 311-323页

作者： WANG GANG CHANG HUI HUANG YAN ZHAO LING CAI YING WANG SHI JIN XIU ZHENG WEN JIANG SHUANG YANG XIN TAI ZHAO WEI HUANG JIAN REN GU National Laboratory for Oncogene and Related Genes, Shanghai Cancer Institute, 25/2200, Xie-tu Rd, Shanghai 200032, harmacy.ankara.edu.tr

To elucidate the molecular pathology underlying the development of hepatocellular carcinoma (HCC), we used 41 highly polymorphic microsatellite markers to examine 55 HCC and corresponding non-tumor liver tissues on chromosome 9, 16 and 17. Loss-of-heterozygosity (LOH) is observed with high frequency on chromosomal region 17p13 (36/55, 65%), 9p21-p23 (28/55, 51%), 16q21-q23 (27/55, 49%) in tumors. Meanwhile, microsatellite instability is rarely found in these microsatellite loci. Direct sequencing was performed to detect the tentative mutation of tumor suppressor genes in these regions: p53, MTS1/p16, and CDH1/E-cadherin. Within exon 5-9 of p53 gene, 14 out of 55 HCC specimens (24%) have somatic mutations, and nucleotide deletion of this gene is reported in HCC for the first time. Mutation in MTS1/pl6 is found only in one tumor case. We do not find mutations in CDH1/E-cadherin. Furthermore, a statistically significant correlation is present between p53 gene mutation and loss of chromosome region 16q21q23 and 9p21-p23, which indicates that synergism between p53 inactivation and deletion of 16q21-q23 and 9p21-p23 may play a role in the pathogenesis of HCC. Genetic aberration in hepatocellular carcinoma

关键词： Hepatocellular carcinoma p53 gene mutation loss of heterozygosity (LOH) microsatellite mark?

DETERMINATION OF COHESIVE ENERGY DENSITY OF UP RESIN BY THE SWELLING METHOD

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Journal of Wuhan University of Technology(Materials Science) 2000年第4期15卷 38-42页

作者：曾黎明 harmacy.ankara.edu.tr

Different types of solvents were chosen, and their solubleexpansion ability to unsaturated polyster- resin was determined. Theprinciple and method of deter- mining the cohesive energy density ofunsaturated polyster re... 详细信息

关键词： unsaturated polyster resin intensity of cohesie energy swelling method

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GREY CLASSIFICATION FOR EVALUATING THE STABILITY OF DANGEROUS ROCK-BLOCK MASSES

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Journal of Wuhan University of Technology(Materials Science) 2000年第1期15卷 73-77页

作者：谢全敏 harmacy.ankara.edu.tr

This paper introduces a grey classifica- tion method forevaluating the stability of dangerous rock- block masses according tothe Grey System Theory. This method is applied to the stability ofthe V~# dangerous rock- block masses of Qingjiang water conservancyproject, and better results are abtained. The method which isadvanced in the article is very single and practical, and it can meetall kinds of project's demands.

关键词： the stability of danagerous rock-block masses grey classification forevaluation Qingjiang water conservancy project