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Progress in Natural Science:Materials International 2005年第11期15卷 988-992页

作者： TANG Fang, LIANG Pei and GAO Xiwu (department of entomology, China Agricultural University, Beijing 100094, China) department of entomology China Agricultural University Beijing 100094 China

The tissue-specific expression of glutathione S-transferases (GSTs) in the cotton bollworm and the expression level induced by 2-tridecanone and quercetin were examined using the methods of biochemistry and the quantitative PCR. The relative expression level of GST mRNA was unanimous with the GSTs activity conjugaging with 1-chloro-2, 4-dimitro-benzene (CDNB) in fat bodies, midguts, heads and integuments of cotton bollworms. The GSTs activity in fat bodies was the highest, then midguts, heads and integuments in turn, which was in consistent with the relative expression level of GST mRNA. The specific activity of GSTs and the relative expression level of GST mRNA could be significantly induced by 2-tridecanone and quercetin, and after the induction the order of the GSTs activity and the relative expression level of GST mRNA in the above four tissues in cotton bollworms was not different from the control. The induction of GSTs by 2-tridecanone was stronger than by quercetin in all four tissues, which was in accordance with the relative expression level of GST mRNA. It suggested that the increase of GSTs activity induced by plant allelochemicals was associated with the elevated expression of GST mRNA in cotton bollworms.

关键词： Helicoverpa armigera glutathione S-transferase tissue-specific expression plant allelochemicals.

Fitness and physiology of Adelges tsugae （Hemiptera： Adelgidae） in relation to the health of the eastern hemlock

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Insect Science 2016年第6期23卷 843-853页

作者： Anne C. Jones Donald E. Mullins Carlyle Brewster James P. Rhea Scott M. Salom department of entomology Pennsylvania State University PA 16801 department of entomology Virginia Tech Blacksburg VA 24061 USDA Forest Service Asheville NC 28804-3454 USA

The hemlock woolly adelgid, Adelges tsugae Annand is an invasive insect that frequently causes hemlock （Tsuga spp.） mortality in the eastern United States. Studies have shown that once healthy hemlocks become infested by the adelgid, nutrients are depleted from the tree, leading to both tree decline and a reduction of the adelgid population. Since A. tsugae is dependent on hemlock for nutrients, feeding on trees in poor health may affect the ability of the insect to obtain necessary nutrients and may consequently affect their physiological and population health. Trees were categorized as lightly or moderately impacted by A. tsugae based on quantitative and qualitative tree health measurements. Population health ofA. tsugae on each tree was determined by measuring insect density and peak mean fecundity; A. tsugae physiological health was determined by measuring insect biomass, total carbon, carbohydrate, total nitrogen, and amino nitrogen levels. Adelges tsugae from moderately impacted trees exhibited significantly greater fecundity than from lightly impacted trees. However, A. tsugae from lightly impacted hemlocks contained significantly greater levels of carbohydrates, total nitrogen, and amino nitrogen. While the results of the physiological analysis generally support our hypothesis that A. tsugae on lightly impacted trees are healthier than those on moderately impacted trees, this was not reflected in the population health measurements. Adelges tsugae egg health in response to tree health should be verified. This study provides the first examination of A. tsugae physiological health in relation to standard A. tsugae population health measures on hemlocks of different health levels.

关键词： Adelges tsugae carbohydrates fecundity nitrogen tree health Tsuga canadensis

Intraguild predation among Scolothrips Iongicornis （Thysanoptera： Thripidae）, Neoseiulus californicus and Typhlodromus bagdasarjani （Acari： Phytoseiidae） under laboratory conditions

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Insect Science 2015年第2期22卷 263-272页

作者： Azadeh Farazmand Yaghoub Fathipour Karim Kamali department of entomology Faculty of Agriculture Tarbiat Modares University Tehran Iran

This study was carried out on the ability of predatory thrips Scolothrips longi- cornis Priesner to feed on 2 phytoseiid species and vice versa. Also the effect of predation of Neoseiulus californicus （McGregor） on Typhlodromus bagdasarjani Wainstein and Aru- tunjan and vice versa was evaluated. The larvae, prepupae, and pupae of thrips and the eggs, larvae, and protonymphs of phytoseiids were selected as intraguild prey. The in- traguild predation （IGP） among S. longicornis and 2 phytoseiid species was unidirectional and in favor of phytoseiids, i.e., S. longicornis was not able to feed on larval stages of 2 phytoseiids. However, N. californicus and T. bagdasarjani fed on the 1st instar larvae （1.39 and 0.80 per day）, 2nd instar larvae （0.87 and 0.55 per day）, prepupae （0.51 and 0.48 per day）, and pupae of thrips （0.51 and 0.49 per day, respectively）. Both phytoseiids fed on eggs, larvae, and protonymphal stages of each other. Females of N. californicus con- sumed more phytoseiid larvae （2.49 per day） than T. bagdasarjani, which consumed 1.08 N. californicus larvae per day. When Tetranychus urticae was presented as an extraguild prey, intensity oflGP between 2 species ofphytoseiids and on larval stages ofS. longicornis reduced significantly. Therefore, it is concluded that （i） IGP existed among the 3 examined species and lack of feeding of S. longicornis on 2 phytoseiid species can be justified by its feeding type （monophagy）, （ii） N. californicus was much more prone to IGP than was T. bagdasarjani.

关键词： intraguild interactions Neoseiulus californicus Scolothrips longicornis,Tetranychus urticae Typhlodromus bagdasarjani

Moth diversity in three biofuel crops and native prairie in Illinois

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Insect Science 2013年第3期20卷 407-419页

作者： Terry Harrison May R. Berenbaum department of entomology University of Ilfinois at Urbana-Champaign Urbana Illinois USA

The expanding demand for biofuel feedstock may lead to large-scale con- scription of land for monoculture production ofbiofuel crops with concomitant substantial negative impacts on biodiversity. We compared moth diversity in light-trap samples from corn, miscanthus, switchgrass and native prairie, to determine whether there is an observ- able relationship between plant species diversity and moth abundance and diversity. Moth alpha diversity was highest in prairie and was higher in switchgrass than in the other two biofuel crops. Beta diversity generally was low among the biofuel crops, and prairie shared lower beta diversity with switchgrass than with corn or miscanthus. Analysis of variance showed no significant differences in moth abundance per species among treatments. The alpha and beta diversity index findings are consistent with those of other studies on arthro- pods in biofuel crops and provide evidence to suggest that large-scale conversion of acreage to biofuel crops may have substantial negative effects on arthropod biodiversity both within the cropping systems and in the surrounding landscape.

关键词： agricultural landscapes biodiversity bioenergy ecosystem services moths prairies

Induction of phenylalanine ammonia-lyase and lipoxygenase in cotton seedlings by mechanical wounding and aphid infestation

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Progress in Natural Science:Materials International 2005年第5期15卷 419-423页

作者： QIN Qiuju, SHI Xueyan, LIANG Pei and GAO Xiwu*(department of entomology, China Agriculture University, Beijing 100094, China) department of entomology China Agriculture University Beijing 100094 China

It has been suggested that infestation of plants causes increases in the activities of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX), key enzymes in the phenolic compounds synthesis pathway and the octadecanoid pathway, respectively. The purpose of this work is to investigate whether the infestation of cotton aphid (Aphis gossypii) and mechanical wound can cause the induction of PAL and LOX activities in cotton seedlings, and whether the induction occurs in healthy seedlings growing nearby the attacked ones. The specific activities of PAL and LOX were measured using spectrophotometric method after aphid infestation and mechanical wounding. Result indicated that PAL activity and LOX activity were greatly induced by mechanical wounding and aphid infestation in cotton seedlings. The induction of PAL and LOX occurred not only in wounded and infested seedlings but also in intact healthy seedlings growing nearby. After exposed to the aphid infestation-induced volatiles, the specific activity of PAL in cotton seedlings increased by 6% at 24 h, 80% at 48 h, 235% at 72 h compared to the control, and the specific activity of LOX increased by 18% at 24 h, 34% at 48 h, 24% at 72 h, respectively. In comparison, the specific activity of PAL in unwounded seedlings exposed to wound-induced volatiles increased by 0.0 at 24 h, 200% at 48 h, 164% at 72 h, respectively and the specific activity of LOX increased by 28% at 24 h, 37% at 48 h, 8% at 72 h, respectively. It suggests that the induced volatiles are involved in plant-plant communication as airborne transferred signals.

关键词： phenylalanine ammonia-lyase lipoxygenase cotton aphid mechanical damage cotton plants.

Biochemical characterization of chitin synthase activity and inhibition in the African malaria mosquito, Anopheles gambiae

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Insect Science 2013年第2期20卷 158-166页

作者： Xin Zhang Kun Yan Zhu department of entomology Kansas State University Manhattan KS 66506 USA

Chitin synthase （CHS） is an important enzyme catalyzing the formation of chitin polymers in all chitin containing organisms and a potential target site for insect pest control. However, our understanding of biochemical properties of insect CHSs has been very limited. We here report enzymatic and inhibitory properties of CHS prepared from the African malaria mosquito, Anopheles gambiae. Our study, which represents the first time to use a nonradioactive method to assay CHS activity in an insect species, determined the optimal conditions for measuring the enzyme activity, including pH, temperature, and concentrations of the substrate uridine diphosphate N-acetyl-D-glucosamine （UDP- GlcNAc） and Mg＋＋. The optimal pH was about 6.5-7.0, and the highest activity was detected at temperatures between 37℃ and 44℃. Dithithreitol is required to prevent melanization of the enzyme extract. CHS activity was enhanced at low concentration of GIcNAc, but inhibited at high concentrations. Proteolytic activation of the activity is significant both in the 500×g supernatant and the 40 000×g pellet. Our study revealed only slight in vitro inhibition ofA. gambiae CHS activity by diflubenzuron and nikkomycin Z at the highest concentration （2.5μmol/L） examined. There was no in vitro inhibition by polyoxin D at any concentration examined. Furthermore, we did not observe any in vivo inhibition of CHS activity by any of these chemicals at any concentration examined. Our results suggest that the inhibition of chitin synthesis by these chemicals is not due to direct inhibition of CHS in A. gambiae.

关键词： Anophele gambiae chitin synthase diflubenzuron enzyme inhibition

Methods for discovery and characterization of cellulolytic enzymes from insects

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Insect Science 2010年第3期17卷 184-198页

作者： Jonathan D. Willis Cris Oppert Juan L. Jurat-Fuentes department of entomology and Plant Pathology University of Tennessee Knoxville Tennessee USA

Cellulosic ethanol has been identified as a crucial biofuel resource due to its sustainability and abundance of cellulose feedstocks. However, current methods to obtain glucose from lignocellulosic biomass are ineffective due to recalcitrance of plant biomass. Insects have evolved endogenous and symbiotic enzymes to efficiently use lignocellulosic material as a source of metabolic glucose. Even though traditional biochemical methods have been used to identify and characterize these enzymes, the advancement of genomic and proteomic research tools are expected to allow new insights into insect digestion of cellulose. This information is highly relevant to the design of improved industrial processes ofbiofuel production and to identify potential new targets for development of insecticides. This review describes the diverse methodologies used to detect, quantify, purify, clone and express cellulolytic enzymes from insects, as well as their advantages and limitations.

关键词： biofuels cellulases cellulase discovery methods cellulase substrate insect digestive fluids lignocellulosic biomass

The influence of Tetranychus cinnabarinus-induced plant defense responses on Aphis gossypii development

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Journal of Integrative Agriculture 2018年第1期17卷 164-172页

作者： MA Guang-min SHI Xue-yan KANG Zhi-jiao GAO Xi-wu department of entomology China Agricultural University College of Agriculture Liaocheng University

Carmine spider mites （Tetranychus cinnabarinus） and cotton aphids （Aphis gossypii） are both serious pests of cotton, and cause reductions in yields of this key agricultural crop. In order to gain insights into how plant defense responses induced by one herbivore species affect the behavior and performance of another, we examined how infestation with T. cinnabarinus influences the development of A. gossypfi using cotton as a model. In this study, we measured the activities of several important biochemical markers and secondary metabolites in the leaves of cotton seedlings responding to infestation by T. cinnabarinus. Furthermore, the influences of T. cinnabarinus infestation on the development ofA. gossypfi in cotton were also examined. Our data showed that the activities of several key defense enzymes, including phenylalanine ammonia-lyase （PAL）, peroxidase （POD）, lipoxygenase （LOX）, and polyphenol oxidase （PPO）, were substantially increased in cotton seedlings responding to spider mite infestation. Further, the contents of gossypol and condensed tannins, key defensive compounds, were significantly enhanced in leaves of cotton seedlings following T. cinnabarinus infestation. Moreover, the T. cinnabarinus-induced production of defense enzymes and secondary metabolites was correlated with infestation density. The developmental periods of A. gossypii on cotton seedling leaves infested with T. cinnabarinus at densities of 10 and 15 individuals cm-2 were 1.16 and 1.18 times that of control, respectively. Meanwhile, the mean relative growth rates of A. gossypfi on cotton leaves infested with T. cinnabarinus at densities of 8, 10 and 15 individuals cm-2 were significantly reduced. Therefore, these data suggested that the developmental periods of A. gossypfi were significantly lengthened and the mean relative growth rates were markedly reduced when cotton aphids were reared on plants infested with high densities of spider mites. This research sheds light on the role that in

关键词： inducible defense cotton Tetranychus cinnabarinus Aphis gossypii interaction

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Comparative life table analysis of Tetranychus urticae （Acari Tetranychidae） on 14 soybean genotypes

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Insect Science 2011年第5期18卷 541-553页

作者： Amin Sedaratian Yaghoub Fathipour Saeid Moharramipour department of entomology Faculty of Agriculture Tarbiat Modares University Tehran Iran

The life table parameters of two-spotted spider mite （TSSM）, Tetranychus urticae Koch, on 14 soybean genotypes （Sari, Sahar, Tellar, Zane, Ks3494, LI7, Dpx, 032, 033, Clark, Hill, 127, Tms and Williams） were evaluated at 28 ±1℃, 65% ± 5% RH and a photoperiod of 16 ： 8 h L ： D. The survivorship data （lx） of adult females were fitted to the Weibull frequency distribution model in all genotypes tested. Based on the Weibull parameters, the survival curve was type I on all genotypes, which indicated that the mortality mostly occurred in old individuals. Furthermore, two mathematical models （Analytis and Enkegaard） were fitted to age-specific fecundity data （mx）. The highest intrinsic rate of increase （rm） was recorded on L17 （0.392） and the lowest values of this parameter were obtained on Tms （0.233）, Hill （0.236）, 032 （0.236）, 127 （0.237） and Clark （0.240）. In addition, net reproductive rate （R0） and finite rate of increase （λ） of the TSSM had the highest value on L17 as 45.521 and 1.475, respectively. The lowest values of these parameters were recorded on Tms as 12.149 and 1.258, respectively. Doubling time （DT） varied significantly on different genotypes and the shortest and longest values of this period were obtained on L17 and 032, respectively. Our findings revealed that Tms, Hill, 032, 127 and Clark were less suitable genotypes, suggesting that they are more resistant to the TSSM than the other genotypes.

关键词： fecundity life table parameters resistance soybean two-spotted spider mite

Ecology and management of the black inch worm, Hyposidra talaca Walker (Geometridae： Lepidoptera) infesting Camellia sinensis (Theaceae)： A review

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Journal of Integrative Agriculture 2017年第10期16卷 2115-2127页

作者： Somnath Roy Soma Das Gautam Handique Ananda Mukhopadhyay Narayanannair Muraleedharan department of entomology Tocklai Tea Research Institute Tea Research Association Jorhat 785008 India entomology Research Unit Department of Zoology University of North Bengal Siliguri 734014 India

The black inch worm, Hyposidra talaca （Walker）（Geometridae： Lepidoptera） has earned considerable importance as a defoliator during last decade in north east India. Characteristic features of H. talaca, Such as switching from wild plants to Camellia sinensis, and completion of 6-8 generations in a year without winter diapause appear to be the major reasons for persistence of the defoliator on C. sinensis. There are reports on the aggressive infestation of the pest on C. sinensis than on alternate host plants and development of high tolerance^resistance to certain insecticides. Till date, more than one hundred alternate host plants have been recorded for H. talaca, but with very few natural enemies. To manage this pest chemically, synthetic pesticides are widely used. Integrated management is recently being encouraged wherein cultural, mechanical, physical, biological and chemical methods have been contemplated. This review collates the work and literature on the ecology of H. talaca and offers an analysis of the problems encountered in managing the defoliator in tea plantations. Further, future directions for better management of H. talaca are suggested.

关键词： Hyposidra talaca black inch worm tea bionomics resistance integrated pest management (IPM)

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