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Vermicompost:In situ retardant of antibiotic resistome accumulation in cropland soils

Journal of Environmental Sciences 2024年第7期141卷 277-286页

作者： Meirui Mu Fengxia Yang Bingjun Han Guisheng Tian Keqiang Zhang Agro-Environmental Protection Institute Ministry of Agriculture and Rural AffairsTianjin 300191China Key Laboratory of Low-carbon Green agriculture in North China Ministry of Agriculture and Rural Affairs P.R.ChinaBeijing 10083China Wuxue City agriculture and rural bureau Wuhan 435400China

The dissemination of antibiotic resistance genes(ARGs)in soil has become a global environmental *** is gaining prominence in agricultural practices as a soil amendment to improve soil ***,its impact on soil ARGs remains unclear when it occurs in *** comprehensively explored the evolution and fate of ARGs and their hosts in the field soil profiles under vermicompost application for more than *** application increased several ARG loads in soil environment but decreased the high-risk bla-ARGs(bla_(ampC),bla_(NDM),and bla_(GES-1))by log(0.04-0.43).ARGs in soil amended with vermicompost primarily occurred in topsoil(approximately 1.04-fold of unfertilized soil),but it is worth noting that their levels in the 40-60 cm soil layer were the same or even less than in the unfertilized *** microbial community structure changed in soil profiles after vermicompost *** application altered the microbial community structure in soil profiles,showing that the dominant bacteria(i.e.,Proteobacteria,Actinobacteriota,Firmicutes)were decreased 2.62%-5.48%with the increase of soil depth.A network analysis further revealed that most of ARG dominant host bacteria did not migrate from surface soil to deep *** particular,those host bacteria harboring high-risk bla-ARGs were primarily concentrated in the surface *** study highlights a lower risk of the propagation of ARGs caused by vermicompost application and provides a novel approach to reduce and relieve the dissemination of ARGs derived from animals in agricultural production.

关键词： Vermicompost application Antibiotic resistance genes Vertical migration Soil profile Agricultural soils

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Effects of Functional Organic Materials on Morphological Indexes,Quality and Yield of Watermelon

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Agricultural Biotechnology 2024年第3期13卷 8-10页

作者： Kun CHEN Shuying CHEN Tao JIANG Pan ZHANG Shangqiu Academy of agriculture and Forestry Sciences Shangqiu 476000China zoucheng bureau of agriculture and rural affairs Zoucheng 273500China

[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of four functional organic materials,namely garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),on the morphological indexes,yield and quality of watermelon were investigated.[Results]Different functional organic materials had different effects on morphological indexes,yield and quality of *** morphological indexes,nutritional quality indexes and yield of watermelon treated with garlic straw and sheep manure compound(T_(3))and onion straw and chicken manure compound(T_(4))were significantly higher than those treated simply with garlic straw(T_(1))and onion straw(T_(2)),and T_(3)performed relatively *** with treatment T_(2),T_(3)showed a stem diameter,vine length and leaf number increasing by 43.05%,46.69%and 40.77%respectively,central sugar and side sugar contents increasing by 11.72%and 21.90%respectively,and a yield increasing by 42.91%,with significant differences from T_(2).[Conclusions]This study provides technical support for high-quality and high-yielding cultivation of watermelon.

关键词： Organic materials Watermelon Morphological index Quality yield

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Biochar Decreases Soil Cadmium(Cd) Availability and Regulates Expression Levels of Cd Uptake/Transport-Related Genes to Reduce Cd Translocation in Rice

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Rice science 2024年第5期31卷 494-498,I0006-I0013页

作者： WANG Han HUANG Qina ZHANG Yan SHAO Guosheng HU Yijun XU Youxiang China National Rice Research Institute Hangzhou 310006China Jiangshan agriculture and rural affairs bureau Quzhou 324100China Longyou agriculture and rural affairs bureau Quzhou 324400China

Biochar has been used as a soil amendment for heavy metal-contaminated soils, and it has the potential to mitigate Cd accumulation in plants. In this study, we used rice straw biochar(RSB) and kitchen waste biochar(KWB) to clarify the effect of biochar on Cd-contaminated neutral soil, the physiological responses to biochar application, and the gene regulatory networks in a rice genotype.

关键词： soil straw soils

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Development of SNP parentage assignment techniques in the yellowfin seabream Acanthopagrus latus

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Acta Oceanologica Sinica 2024年第2期43卷 151-155页

作者： Hongbo Zhao Liangmin Huang Jing Zhang Songyuan You Qingmin Zeng Xiande Liu Fisheries College of Jimei University Xiamen 361021China Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture and Rural AffairsXiamen 361021China bureau of agriculture and rural affairs of Wudang District Guiyang CityGuizhou ProvinceGuiyang 550018China Fisheries Research Institute of Fujian Xiamen 361000China

Acanthopagrus latus is an essential aquaculture species on the south coast of ***,there is a lack of systematic breeding of ***,which considerably limits the sustainable development of *** a result,genetic improvements are urgently needed to breed new strains of *** with rapid growth and strong resistance to *** selective breeding,it is necessary to estimate the genetic parameters of the target trait,which in turn depends on an accurate disentangled pedigree for the selective ***,it is necessary to establish the parentage assignment technique for *** this study,95 individuals selected from their parents and their 14 families were used as experimental *** were developed by genome resequencing,and highly polymorphic SNPs were screened on the basis of optimized filtering parameters.A total of 14392738 SNPs were discovered and 205 SNPs were selected for parentage assignment using the CERVUS *** the model where the gender of the parents is known,the assignment success rate is 98.61%for the male parent,97.22%for the female parent,and 95.83%for the parent *** the model where the gender of the parents is unknown,the assignment success rate is 100%for a single parent and 90.28%for the parent *** results of this study were expected to serve as a reference for the breeding of new varieties of ***.

关键词： Acanthopagrus latus parentage assignment SNP Genome re-sequencing

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Spatiotemporal evolution and assembly processes of ammonia-oxidizing prokaryotic communities in 1 000-year-old coastal reclaimed soils

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Pedosphere 2024年第4期34卷 709-725页

作者： Sarfraz HUSSAIN Yifan YIN Senlin LIU Shanshan YAN Dongjie CHEN Hui CAO Feng WANG Key Laboratory of Agricultural Environmental Microbiology Ministry of Agriculture and Rural AffairsCollege of Life SciencesNanjing Agricultural UniversityNanjing 210095China Institute of Eco-Environmental Sciences Ningbo Academy of Agricultural SciencesNingbo 315040China

Coastal marshes are transitional areas between terrestrial and aquatic *** are sensitive to climate change and anthropogenic *** recent decades,the reclamation of coastal marshes has greatly increased,and its effects on microbial communities in coastal marshes have been studied with great *** of these studies have explained the short-term spatiotemporal variation in soil microbial community ***,the impact of reclamation on the community composition and assembly processes of functional microbes(e.g.,ammonia-oxidizing prokaryotes)is often *** this study,using quantitative polymerase chain reaction and the Ion S5™XL sequencing platform,we investigated the spatiotemporal dynamics,assembly processes,and diversity patterns of ammonia-oxidizing prokaryotes in 1000-year-old reclaimed coastal salt *** taxonomic and phylogenetic diversity and composition of ammonia oxidizers showed apparent spatiotemporal variations with soil *** null modelling-based analysis showed that across all sites,the archaeal ammonia-oxidizing community was assembled by a deterministic process(84.71%),and deterministic processes were also dominant(55.2%)for ammonia-oxidizing bacterial communities except for communities at 60 years of *** assembly process and nitrification activity in reclaimed soils were positively *** abundance of the amoA gene and changes in ammonia-oxidizing archaeal and bacterial diversities significantly affected the nitrification activity in reclaimed *** findings suggest that long-term coastal salt marsh reclamation affects nitrification by modulating the activities of ammonia-oxidizing microorganisms and regulating their community structures and assembly *** results provide a better understanding of the effects of long-term land reclamation on soil nitrogen-cycling microbial communities.

关键词： ammonia oxidizer beta nearest taxon index coastal salt marshes community assembly deterministic process nitrification rate stochastic process

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Litter Productivity and Nutrient Return Characteristics of Three Typical Forest Stands in Golden Mountain

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Open Journal of Applied Sciences 2024年第2期14卷 353-370页

作者： Fengchen Yan Jiang Zhu Juyang Wu Jinshi Chen Zijun Tian School of Forestry and Horticulture Hubei Minzu University Enshi China agriculture and rural affairs bureau of Enshi Tujia and Miao Autonomous Prefecture Enshi China

Objective: The paper aims to analyze the dynamic characteristics of litter production and nutrient return of the forest ecosystems in subtropical areas, and provide a theoretical basis for the nutrient cycling study in southwest Hubei Province and carbon sink function of the whole forest ecosystem. Methods: Three typical forest stands (Chinese fir plantation, Cryptomeria fortunei plantation and evergreen and deciduous broad-leaved mixed forest) in Golden Mountain Forest Farm in southwest Hubei Province were investigated and monitored continuously for the litter types and productivity and nutrient return. Results: The annual litter productivity of the three forest stands ranged from 161.77 to 396.26 kg·hm-2;Litters of branches, leaves and reproductive organs accounted for 14.14% - 20.85%, 33.26% - 78.33%, 7.52% - 42.18% of the total, respectively;The litter productivity and total litter productivity of each composition in the three forest stands show unimodal or bimodal changes over months, and the total litter productivity reached the highest value in January, April and October respectively. For different nutrient contents of the three forest stands, the common feature is C > N. The order of nutrient return amount from greatest to least is evergreen and deciduous broad-leaved mixed forest, Cryptomeria fortunei plantation and Chinese fir plantation. For different nutrient return amounts, the common feature is C > N, and the nutrient return amounts are 76.51-180.69 kg·hm-2 and 2.3 - 5.71 kg·hm-2 respectively. Conclusion: The annual litter productivity and nutrient return amount of the evergreen and deciduous broad-leaved mixed forest are the highest among the three forest stands. Therefore, protecting the evergreen and deciduous broad-leaved mixed forest and studying the litter changes of Chinese fir plantation and Cryptomeria fortunei plantation are of far-reaching significance for the development of sustainable forest management in t

关键词： Golden Mountain Litter Productivity Nutrient Return Amount Nutrient Content

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Phenotypic Characterization and QTL/Gene Identification for Internode Number and Length Related Traits in Maize

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American Journal of Plant Sciences 2024年第7期15卷 467-485页

作者： Jing Li Fengjuan Gu Guoqiang Wang Yingyi Zhang Xiangling Gong Wei Wei Xianchuang Zhang Lin Liu Hameed Gul Hong Duan Chaoxian Liu Qianlin Xiao Zhizhai Liu College of Agronomy and Biotechnology/Maize Research Institute Southwest University Chongqing China Health Commission of Suixi County Huaibei China agriculture and rural bureau of Qinglong County Qinglong County China Yunyang Committee of agriculture and rural affairs Chongqing China

Internode number and length are the foundation to constitute plant height, ear height and the above-ground spatial structure of maize plant. In this study, segregating populations were constructed between EHel with extremely low ear height and B73. Through the SNP-based genotyping and phenotypic characterization, 13 QTL distributed on the chromosomes (Chrs) of Chr1, Chr2, Chr5-Chr8 were detected for four traits of internode no. above ear (INa), average internode length above ear (ILaa), internode no. below ear (INb), and average internode length below ear (ILab). Phenotypic variation explained (PVE) by a single QTL ranged from 6.82% (qILab2-2) to 12.99% (qILaa5). Zm00001d016823 within the physical region of qILaa5, the major QTL for ILaa with the largest PVE was determined as the candidate through the genomic annotation and sequence alignment between EHel and B73. Product of Zm00001d016823 was annotated as a WEB family protein homogenous to At1g75720. qRT-PCR assay showed that Zm00001d016823 highly expressed within the tissue of internode, exhibiting statistically higher expression levels among internodes of IN4 to IN7 in EHel than those in B73 (P Zm00001d016823 might provide novel insight into molecular mechanism beyond phytohormones controlling internode development in maize.

关键词： Maize (Zea mays L.) Internode No. Average Internode Length Phenotypic Characterization Candidate Gene Discovery

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Mapping upland crop–rice cropping systems for targeted sustainable intensification in South China

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The Crop Journal 2024年第2期12卷 614-629页

作者： Bingwen Qiu Linhai Yu Peng Yang Wenbin Wu Jianfeng Chen Xiaolin Zhu Mingjie Duan Key Laboratory of Spatial Data Mining&Information Sharing of Ministry of Education Academy of Digital China(Fujian)Fuzhou UniversityFuzhou 350116FujianChina Key Laboratory of Agricultural Remote Sensing Ministry of Agriculture and Rural AffairsBeijing 100081China Department of Land Surveying and Geo-Informatics The Hong Kong Polytechnic UniversityKowloon 999077Hong KongChina

Upland crop-rice cropping systems(UCR)facilitate sustainable agricultural *** UCR cultivation mapping is needed to ensure food security,sustainable water management,and rural ***,datasets describing cropping systems are limited in spatial coverage and crop *** UCR is more challenging than crop identification and most existing approaches rely heavily on accurate phenology calendars and representative training samples,which limits its applications over large *** describe a novel algorithm(RRSS)for automatic mapping of upland crop-rice cropping systems using Sentinel-1 Synthetic Aperture Radar(SAR)and Sentinel-2 Multispectral Instrument(MSI)*** indicator,the VV backscatter range,was proposed to discriminate UCR and another two indicators were designed by coupling greenness and pigment indices to further discriminate tobacco or oilseed *** RRSS algorithm was applied to South China characterized by complex smallholder rice cropping systems and diverse topographic *** study developed 10-m UCR maps of a major rice bowl in South China,the Xiang-Gan-Min(XGM)*** performance of the RRSS algorithm was validated based on 5197 ground-truth reference sites,with an overall accuracy of 91.92%.There were7348 km^(2) areas of UCR,roughly one-half of them located in *** UCR was represented mainly by oilseed-UCR and tobacco-UCR,which contributed respectively 69%and 15%of UCR *** patterns accounted for only one-tenth of rice production,which can be tripled by intensification from single rice *** to complex and fragmented subtropical regions suggested the spatiotemporal robustness of the RRSS algorithm,which could be further applied to generate 10-m UCR datasets for application at national or global scales.

关键词： Cropping-pattern mapping Paddy rice Sentinel-1/2 China Sustainable intensification

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Effects of Three Humic Acid Foliar Fertilizers on Quality and Photosynthetic Characteristics of Hawthorn

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Asian Agricultural Research 2024年第7期16卷 48-52页

作者： Jia WANG Xinyu LUO Heli SHANG Dongsheng LAN Qi LIU Jingtao ZHU College of Horticultural Science&Technology Hebei Normal University of Science&TechnologyQinhuangdao 066000China bureau of agriculture and rural affairs of Kuancheng Manchu Autonomous County Chengde 067300China Hebei(Chengde)Hawthorn Industry Technology Research Institute Chengde 067304China

[Objectives]To investigate the effects of three foliar fertilizers on photosynthetic characteristics,fruit quality and yield of hawthorn.[Methods]Taking hawthorn foliar fertilization as the reference,the photosynthetic rate,transpiration rate,intercellular CO 2 concentration,stomatal conductance and single fruit weight of hawthorn leaves were measured under different concentrations of foliar fertilizer.[Results]The results showed that the photosynthetic rate,transpiration rate and stomatal conductance of hawthorn leaves increased significantly,while the intercellular CO 2 concentration ***,the fish protein peptide foliar fertilizer performed best,with net photosynthetic rate and transpiration rate increased by 57.22%and 57.51%,*** the three foliar fertilizers significantly reduced the intercellular CO 2 *** addition,fertilization significantly increased the single fruit weight of hawthorn,and the effect of fermented fulvic acid foliar fertilizer was the most significant,with the highest growth rate of 68.49%.[Conclusions]Spraying foliar fertilizer significantly increased the content of Vc,titratable acid,anthocyanin and soluble solids of hawthorn fruit,among which fermented fulvic acid foliar fertilizer had the optimal effect.

关键词： Hawthorn Humic acid Fulvic acid Fish protein peptide Leaf function Fruit quality

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The influences of canopy temperature measuring on the derived crop water stress index

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中国生态农业学报（中英文） 2024年第9期32卷 1503-1519页

作者： WANG Hongxi LI Fei SHEN Hongtao LI Mengyu YIN Gongchao FANG Qin SHAO Liwei Marketing Service Center of the State Grid Hebei Electric Power Co. Ltd.Shijiazhuang 050035China Nanpi agriculture and rural affairs bureau Nanpi 061500China College of Agronomy Hebei Agricultural UniversityBaoding 071000China Center for Agri-cultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China

Crop water stress index(CWSI)is widely used for efficient irrigation *** canopy temperature(T_(c))measurement is necessary to derive a reliable *** objective of this research was to investigate the influences of atmospheric conditions,settled height,view angle of infrared thermography,and investigating time of temperature measuring on the performance of the *** irrigation treatments were used to create different soil water conditions during the 2020-2021 and 2021-2022 winter wheat-growing *** CWSI was calculated using the CWSI-E(an empirical approach)and CWSI-T(a theoretical approach)based on the T_(c).Weather conditions were recorded continuously throughout the experimental *** results showed that atmospheric conditions influenced the estimation of the CWSI;when the vapor pressure deficit(VPD)was>2000 Pa,the estimated CWSI was related to soil water *** height of the installed infrared thermograph influenced the T_(c)values,and the differences among the T_(c)values measured at height of 3,5,and 10 m was smaller in the afternoon than in the ***,the lens of the thermometer facing south recorded a higher T_(c)than those facing east or north,especially at a low height,indicating that the direction of the thermometer had a significant influence on T_(c).There was a large variation in CWSI derived at different times of the day,and the midday measurements(12:00-15:00)were the most reliable for estimating *** linear relationships were found between the transpiration rate and CWSI-E(R^(2)of 0.3646-0.5725)and CWSI-T(R^(2)of 0.5407-0.7213).The relations between fraction of available soil water(FASW)with CWSI-T was higher than that with CWSI-E,indicating CWSI-T was more accurate for predicting crop water *** addition,The R^(2)between CWSI-T and FASW at 14:00 was higher than that at other times,indicating that 14:00 was the optimal time for using the CWSI for crop water status *** hi

关键词： Canopy temperature Measuring time Measuring height and direction Crop water stress index

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