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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi Qiu Mingzhu Yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng Han Bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

Hydrogen-bonded organic framework-ionic liquid composite quasisolid electrolyte for high-performance lithium battery

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Nano Research 2025年第1期18卷 332-340页

作者： Junmei Zhang Yatao Wu Chenye Wang Yong Liu Zhirong Yang Shiyue Zhou Wenpeng Li Wenjia Wu Jingtao Wang School of Chemical Engineering Zhengzhou UniversityZhengzhou 450001China

Ionic liquids(ILs)hold great promise as highperformance electrolyte material due to their unique advantages including nonvolatility,high thermal stability and high ionic ***,the IL-based electrolytes always suffer from serious ion aggregation and high viscosity at low temperatures,leading to significantly decline in ionic ***,hydrogen-bonded organic framework-ionic liquid composite quasi-solid electrolyte(high temperature treatment(HT)-HOF-IL CQSE)was prepared through confining the IL electrolytes(ILEs)into the pore of HOF lamellar *** weak hydrogen bonding interactions within HOF nanosheets,together with the generated interactions between ILE and HOF,enable uniform and continuous distribution of ILE in HOF lamellar *** effectively inhibits the ion migration of ILE,which meanwhile serves as Li+transfer sites,affording high ionic conductivity of 5.7×10^(-5)S·cm-1 at-60 ℃,with high lithium-ion transference number of 0.69,whereas ILEs usually lose ionic conduction ability at such low *** assembled Li symmetrical cell can stably cycle at 0.2 mA·cm^(-2)and^(-2)0 ℃ for more than 1500 *** LiFePO_(4)|HT-HOF-IL CQSE|Li cell shows excellent cycling performance at 0.5 C at a wide temperature range of^(-2)0 to 60 ℃.This work may pave a new avenue for the development of high-performance IL-based composite electrolytes.

关键词： ionic liquid hydrogen-bonded organic framework low-temperature ionic conductivity lithium-ion transference number quasi-solid electrolyte

Nanoparticles for the treatment of spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1665-1680页

作者： Qiwei Yang Di Lu Jiuping Wu Fuming Liang Huayi Wang Junjie Yang Ganggang Zhang Chen Wang Yanlian Yang Ling Zhu Xinzhi Sun Department of Orthopedic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyCAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina University of Chinese Academy of Sciences BeijingChina Department of Neurosurgery The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Spinal cord injuries lead to significant loss of motor, sensory, and autonomic functions, presenting major challenges in neural regeneration. Achieving effective therapeutic concentrations at injury sites has been a slow process, partly due to the difficulty of delivering drugs effectively. Nanoparticles, with their targeted delivery capabilities, biocompatibility, and enhanced bioavailability over conventional drugs, are garnering attention for spinal cord injury treatment. This review explores the current mechanisms and shortcomings of existing treatments, highlighting the benefits and progress of nanoparticle-based approaches. We detail nanoparticle delivery methods for spinal cord injury, including local and intravenous injections, oral delivery, and biomaterial-assisted implantation, alongside strategies such as drug loading and surface modification. The discussion extends to how nanoparticles aid in reducing oxidative stress, dampening inflammation, fostering neural regeneration, and promoting angiogenesis. We summarize the use of various types of nanoparticles for treating spinal cord injuries, including metallic, polymeric, protein-based, inorganic non-metallic, and lipid nanoparticles. We also discuss the challenges faced, such as biosafety, effectiveness in humans, precise dosage control, standardization of production and characterization, immune responses, and targeted delivery in vivo. Additionally, we explore future directions, such as improving biosafety, standardizing manufacturing and characterization processes, and advancing human trials. Nanoparticles have shown considerable progress in targeted delivery and enhancing treatment efficacy for spinal cord injuries, presenting significant potential for clinical use and drug development.

关键词： antioxidants axon regeneration biocompatible materials drug carriers nanoparticles nerve regeneration neuroinflammatory diseases neuroprotection spinal cord injury stem cells

Boosting charge extraction and efficiency of inverted perovskite solar cells through coordinating group modification at the buffer layer/cathode interface

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Nano Research 2025年第1期18卷 415-423页

作者： Zhiqing Liang Ziqiu Ren Ziyu Wang Nan Yan Ling Li Bo Zhang Yanlin Song College of Chemistry Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education Shaanxi Key Laboratory for Advanced Energy DevicesShaanxi Engineering Lab for Advanced Energy Technology School of Materials Science and EngineeringShaanxi Normal UniversityXi’an 710119China Key Laboratory of Green Printing Institute of ChemistryChinese Academy of Sciences(ICCAS)Beijing Engineering Research Center of Nanomaterials for Green Printing TechnologyNational Laboratory for Molecular Sciences(BNLMS)Beijing 100190China

In inverted perovskite solar cells (PSCs),effective modification of the interface between the metalcathode and electron transport layer (ETL) is crucial forachieving high performance and stability. Herein, sulfonatedbathocuproine, commonly known as disodium bathocuproinedisulfonate (BCDS), was employed as a cathode buffer layerto address the interfacial issues at the [6,6]-phenyl-C61-butyricacid methyl ester (PCBM)/Ag interface. BCDS possesses theability to form coordinate bonds with Ag electrodes. Theutilization of the BCDS buffer layer enhanced the chargeextraction capability at the cathode interface whilesimultaneously achieving interfacial defect passivation,improving interfacial contact and increasing the built-in electricfield. Consequently, a power conversion efficiency (PCE) of25.06% was achieved. Furthermore, owing to the excellent filmforminguniformity of BCDS on PCBM, the stability of thedevice was also improved. After storage in dry air for morethan 2000 h, the device maintained 96% of its initial efficiency. This work underscores the remarkable potential of tailoringcoordination groups to enhance charge extraction efficiency at the ETL-cathode interface, unveiling a promising newfrontier in buffer layer development and performance optimization strategies for PSCs.

关键词： perovskite solar cells buffer layer bathocuproine disulfonate coordination group modification electron transport layer(ETL)-cathode interface

Study on Identification of Salmonella in Textiles with LAMP-MALDI TOF MS

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口岸卫生控制 2019年第4期24卷 17-26页

作者： Li Ke Guo Huiqing Wang Yongliang zhengzhou customs technology center

Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of LAMP primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.

关键词： Salmonella InvA Gene LAMP MALDI TOF MS

Absolute quantitative detection of genetically modified soybean MON87708×MON89788 with stacked traits by digital polymerase chain reaction

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Oil Crop Science 2022年第4期7卷 180-188页

作者： Junyi Xu Xin Li Jinglian Bai Ying Liu Shaojie Wang Yueting Liu Chunguang Yang technology center of Dalian customs District DalianChina Logistics Management center of Dalian customs District DalianChina

The main advantage of digital PCR(dPCR) is that it facilitates absolute quantification of the target without reference to the standard/calibration *** droplet dPCR has a three-color staining detection function,which enables multiplex PCR *** this study,this technique was used to establish triple dPCR detection for the genetically modified soybean MON87708 × MON89788 with stacked *** absolute quantitative detection was accomplished for the genomic DNA extracted from the homogenized seeds of GM stack MON87708× MON89788 *** results can serve as a reference for the absolute quantitative detection of stacked events of genetically modified crops.

关键词： Absolute quantification Digital PCR Genetically modified soybean MON87708×MON89788 Stacked event

Joint TDOA, FDOA and differential Doppler rate estimation： Method and its performance analysis

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Chinese Journal of Aeronautics 2018年第1期31卷 137-147页

作者： Dexiu HU Zhen HUANG Shangyu ZHANG Jianhua LU Space technology Research center Tsinghua University Beijing 100084 China zhengzhou Information technology Institute Zhengzhou 450002 China

Considering the estimation accuracy reduction of Frequency Difference of Arrival （FDOA） caused by relative Doppler companding, a joint Time Difference of Arrival （TDOA）, FDOA and differential Doppler rate estimation method is proposed and its Cramer-Rao low bound is derived in this paper. Firstly, second-order ambiguity function is utilized to reduce the dimensionality and estimate initial TDOA and differential Doppler rate. Secondly, the TDOA estimation is updated and FDOA is obtained using cross ambiguity function, in which relative Doppler com- panding is compensated by the existing differential Doppler rate. Thirdly, differential Doppler rate estimation is updated using cross estimator. Theoretical analysis on estimation variance and Cramer-Rao low bound shows that the final estimation of TDOA, FDOA and differential Doppler rate performs well at both low and high signal-noise ratio, although the initial estimation accuracy of TDOA and differential Doppler rate is relatively poor under low signal-noise ratio conditions. Simulation results finally verify the theoretical analysis and show that the proposed method can overcome relative Doppler companding problem and performs well for all TDOA, FDOA and differential Doppler rate estimation.

关键词： Differential Doppler rate Frequency difference of arrival Passive location Performance analysis Time difference of arrival

Simple synthesis of sandwich-like SnSe2/rGO as high initial coulombic efficiency and high stability anode for sodium-ion batteries

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Journal of Energy Chemistry 2020年第7期29卷 71-77,I0003页

作者： Tingting Wang Kaiwei Yang Juan Shi Shangrui Zhou Liwei Mi Hongping Li Weihua Chen Green Catalysis center and College of ChemistryZhengzhou UniversityZhengzhou 450001HenanChina center for Advanced Materials Research Zhongyuan University of TechnologyZhengzhou 450007HenanChina

Metal selenides owing to their high theoretical capacity and good conductivity are considered as one of the potential candidates for the anode materials of sodium-ion batteries(SIBs).However,their practical applications are greatly restricted by the poor cycling performances and complicated synthesis *** this work,a sandwich-like Sn Se2/reduced graphene oxide(r GO)composite with a small amount of r GO(7.3%)is synthesized by a simple one-pot solvothermal *** as-synthesized Sn Se2/r GO shows improved initial coulombic efficiency(ICE)of 73.7%,high capacity of 402.0 m Ah g-1 after 150 cycles at 0.1 A g-1 with a retention of 86.2%and outstanding rate *** abundant Sn-O-C bonds of synthesized material not only accelerate the charge transfer at the interface but also enhance the mechanical strength to accommodate volume variation and prevent active material loss during ***,the compact structure leads to thin solid electrolyte interface(SEI)so that high initial coulombic efficiency was ***,full cells are assembled to test its potential *** work offers a simple method to synthesize Sn Se2/r GO as a candidate anode for SIBs.

关键词： Sodium-ion batteries Initial coulombic effciency Ex-situ XRD SEI Tin diselenide

Facile synthesis of hierarchical Na2Fe(SO4)2@rGO/C as high-voltage cathode for energy density-enhanced sodium-ion batteries

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Journal of Energy Chemistry 2020年第11期29卷 387-394页

作者： Ge Yao Xixue Zhang Yongliang Yan Jiyu Zhang Keming Song Juan Shi Liwei Mi Jinyun Zheng Xiangming Feng Weihua Chen Green Catalysis center and College of ChemistryZhengzhou UniversityZhengzhou 450001HenanChina center for Advanced Materials Research Zhongyuan University of TechnologyZhengzhou 450007HenanChina

Fe-based sulfates are ideal cathode candidates for sodium-ion batteries(SIBs) owing to their high operating voltage and low cost but suffer from the nature of poor power performance. Herein, a hierarchical porous Na2Fe(SO4)2@reduced graphene oxide/carbon dot(Na2Fe(SO4)2@rGO/C) with low carbon content(4.12 wt%) was synthesized via a facile homogeneous strategy benefiting for engineering application,which delivers excellent sodium storage performance(high voltage plateau of 3.75 V, 85 m Ah g-1 and330 Wh kg-1 at 0.05 C;5805 W kg-1 at 10 C) and high Na+diffusion coefficient(1.19 × 10-12 cm2 s-1).Moreover, the midpoint voltage of assembled full cell could reach 3.0 V. The electron transfer and reaction kinetics are effectively boosted since the nanoscale Na2Fe(SO4)2 is supported by a robust crosslinked carbon matrix with rGO sheets and carbon dots. The slight rGO sheets sufficiently enhance the electron transfer like a current collecter and restrain the aggregation, as well as ensure smooth ion channels. Meanwhile, the carbon dots in the whole space connect with Na2Fe(SO4)2 and help rGO to promote the conductivity of the electrode. Ex-situ X-ray powder diffraction and X-ray photoelectron spectrometry analysis confirm the high reversibility of this sodiation/desodiation process.

关键词： Sodium ion batteries High-voltage cathode Fe-based sulfates Full cell Hierarchical structure