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Risk assessment of Bacillus cereus in lettuce grown in open-field and greenhouse farming systems in China: A comparative study

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FOOD QUALITY AND SAFETY 2024年第8期8卷 268-277页

作者： Qu, Yang Yu, Shenghao Gu, Haotian Wang, Wen Wang, Wenbo He, Zhaoying Shan, Hong Gao, Min Zhao, Xin Wei, Chao Tao, Tingting Zhao, Guanghua Zhou, Changyan Suo, Yujuan Shanghai acad agr sci Inst Agrofood Stand & Testing Technol Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Shanghai Peoples R China Shanghai Municipal Adm Market Regulat Informat Applicat Res Ctr Shanghai Peoples R China Shanghai acad agr sci Ecoenvironm Protect Res Inst Shanghai Peoples R China zhejiang acad agr sci Inst Agroprod Safety & Nutr MOA Lab Qual & Safety Risk Assessment Agroprod Han Hangzhou Peoples R China Shandong acad agr sci Inst Agr Stand & Testing Technol Agriprod Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Jinan Peoples R China Beijing acad agr & Forestry sci Inst Qual Stand & Testing Technol Beijing Peoples R China Heilongjiang acad agr sci Qual & Safety Inst Agr Prod Harbin Peoples R China Chinese acad agr sci Inst Food Sci & Technol Beijing Peoples R China Tianjin acad agr sci Inst Germplasm Resources & Biotechnol Tianjin Peoples R China Sichuan acad agr sci Inst Qual Stand & Testing Technol Agroprod Lab Qual & Safety Risk Assessment Agroprod Minist Agr & Rural Affairs Chengdu Peoples R China Jiangsu acad agr sci Inst Food Safety & Nutr Key Lab Control Technol & Stand Agroprod Safety & Minist Sci & Technol Nanjing Peoples R China Henan acad & agr sci Res Inst Agr Qual Stand & Testing Tech Zhengzhou Peoples R China

Objectives: Bacillus cereus isolates from vegetables can cause diarrheal syndrome food poisoning. Lettuce, the most highly consumed ready-to-eat vegetable, poses a significant risk of B. cereus exposure. Hence, B. cereus monitoring and evaluation during cultivation are essential for preventing foodborne illnesses caused by this food-pathogen combination. However, the impact of different cultivation practices on B. cereus contamination in lettuce currently remains unclear. Furthermore, a validity indicator for the experimental models cannot be effectively validated under field conditions. Materials and Methods: Herein, we assessed the risk of B. cereus exposure using lettuce grown under two different cultivation farming systems in China (open-field versus greenhouse) using a quantitative microbial risk assessment model. Tukey's mean comparison method and kernel density estimation frequency distribution statistics were used for model validation using environmental samples and lettuce. Results: The mean concentration of B. cereus in open-field-grown lettuce was significantly higher than that in greenhouse-grown lettuce (P0.05), verifying the accuracy of the risk model. Conclusion: Soil solarization, complete manure treatment, usage of mulch films, and irrigation water quality should be considered in agricultural practices to control B. cereus concentrations in lettuce. Overall, we found that B. cereus was more abundant in open-field-grown lettuce, and we provide a risk evaluation framework that can guide farmers and policy-makers to control B. cereus contamination in farm-to-fork lettuce chains in China.

关键词： Diarrheal syndrome food poisoning ready-to-eat vegetable greenhouse microbial risk assessment

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Starch RVA profile parameters of rice are mainly controlled by Wx gene

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Chinese science Bulletin 1999年第22期44卷 2047-2051页

作者： Jinsong Bao Ping He Yingwu Xia Ying Chen Lihuang Zhu Chinese acad sci Inst Genet Beijing 100101 Peoples R China zhejiang Univ Inst Nucl Agr Sci Hangzhou 310029 Peoples R China

Quantitative trait locus (QTLs) mapping for rapid visco analyser (RVA) profile parameters has been carried out by using a double haploid (DH) population derived from a cross between indica variety Zhai-Ye-Qing 8 and j’aponica variety Jing-Xi 17 and its genetic linkage map. The results indicate that the segregation of the RVA profiles is continually distributed a-mong the DH lines, and some DH lines show transgressive segregation for all the parameters.

关键词： rice RVA profile quantitative trait locus (QTL ) doubled hiploid (DH ) population.

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Preparation and characteristics of TEMPO-oxidized cellulose nanofibrils from bamboo pulp and their oxygen-barrier application in PLA films

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Frontiers of Chemical science and Engineering 2017年第4期11卷 554-563页

作者： Wu, Bozhen Geng, Biyao Chen, Yufei Liu, Hongzhi Wu, Qiang Li, Guangyao zhejiang Univ Technol Coll Mat Sci & Engn Hangzhou 310014 Zhejiang Peoples R China zhejiang Prov Collaborat Innovat Ctr Bamboo Resou Hangzhou 311300 Zhejiang Peoples R China zhejiang agr & Forestry Univ Sch Engn Hangzhou 311300 Zhejiang Peoples R China

Bleached bamboo kraft pulp was pretreated by 2,2,6,6-tetramethylpiperidine-l-oxy radical （TEMPO）- mediated oxidation using a TEMPO/NaBr/NaC10 system at pH = 10 in water to facilitate mechanical disintegration into TEMPO-oxidized cellulose nanofibrils （TO-CNs）. A series of TO-CNs with different carboxylate contents were obtained by varying amounts of added NaC10. An increase in carboxylate contents results in aqueous TO-CN dispersions with higher yield, zeta potential values, and optical transparency. When carboxylate groups are introduced, the DPv value of the TO-CNs remarkably decreases and then levels off. And the presence of hemicellulose in the pulp is favorable to TEMPO oxidization. After the oxidization, the native cellulose I crystalline structure and crystal size of bamboo pulp are almost maintained. TEM micrographs revealed that the degree of nanofibrillation is directly proportional to the carboxylate contents. With increasing carboxylate con- tents, the free-standing TO-CN films becomes more transparent and mechanically stronger. The oxygen permeability of PLA films drastically decreases from 355 for neat PLA to 8.4 mL-1 m a. d-1 after coating a thin layer of TO-CN with a carboxylate content of 1.8 mmol.g1. Therefore, inexpensive and abundant bamboo pulp would be a promising starting material to isolate cellulose nanfibrils for oxygen-barrier applications.

关键词： bamboo TEMPO cellulose nanofibrils oxy-gen barrier

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Study of the Laser-MIG Hybrid Welded Al-Mg Alloy

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稀有金属材料与工程 2011年第S4期40卷 115-119页

作者： Tan Bing,Wang Youqi,Cheng Donggao,Liu Hongwei,Ma Zhihua Welding Material and Engineering,Ningbo Branch of China academy of Ordnance science,Ningbo 315103,China China acad Ordnance sci Ningbo Branch Ningbo 315103 Zhejiang Peoples R China

The technology of laser-MIG hybrid welding is hotspot in welding researched field at *** can improve the velocity of the welding,reduce the distortion of the welding,optimize the structure of the welding joint and etc..The 5052 aluminum alloy of the 10mm thick was welded by the laser-MIG hybrid *** structure,the alloy elements profile and the mechanical property of the welded joint are researched by the optical microscope,SEM,sclerometer and etc..The results showed:The medium thick Al alloy was welded in high speed by the laser-MIG hybrid *** appearance of the welding joint is *** weld joint and the weld interface are *** intenerate region in the welding joint is *** tensile strength in welding joint has achieved 94.4% of that in base metal.

关键词： aluminum alloy laser-MIG hybrid welding microstructure mechanical property

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Cloning and characterization of a C genome-specific repetitive sequence in Aegilops caudata L.

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Chinese science Bulletin 1999年第14期44卷 1301-1306页

作者： Xiuying Kong Ronghua Zhou Yuchen Dong Jizeng Jia Chinese acad agr sci Minist Agr Key Lab Crop Germplasm & Biotechnol Beijing 100081 Peoples R China

pAeca212 is 204 bp in length, and the G + C content is 51%. It disperses on all seven chromosome pairs of Aegilops caudata except centromeres and secondary *** with the 316893 DNA sequences registered in Genbank/EMBL/DDJB/PDB, pAeca212 is a new C-genome specific repetitive sequence. The results of genomic specificity analysis of pAeca212 show that there are no hybridization signals detected in all donor Poaceae plants except in rye. pAeca212 is a very useful molecular marker in the study of the origin of Triticeace and the detection of C chromatin in wheat background.

关键词： Aegilops caudata genome specific repetitive sequence.

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Tribological Behavior of Magnesium Alloy by Composite Treatment

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稀有金属材料与工程 2011年第S3期40卷 290-293页

作者： Zheng Shunqi1, Zhang Gaohui2, Huang Guoqing2, Xu Peng2, Li Gen2 1 Ningbo Branch of China academy of Ordnance science, Ningbo 315103, China 2 China Jiliang University, Hangzhou 310018, China China acad Ordnance sci Ningbo Branch Ningbo 315103 Zhejiang Peoples R China China Jiliang Univ Hangzhou 310018 Zhejiang Peoples R China

A compact and homogeneous composite membranous layer was coated on the surface of AZ31 magnesium alloy by a multiple treatment of micro-arc oxidation, magnetron sputtering, and ion implantation in order to improve the wear-resistance performance and micro-arc oxidation surface of the magnesium alloy. X-ray diffraction (XRD) and metallurgical microscopy as well as scanning electron microscopy (SEM) were used to identify the phases structures of the coating and worn surface. The dry sliding wear behavior of the composite membranous layer was performed on WTM-2E tribological and wear tester. The results show that the ceramic coating formed by micro-arc oxidation is not uniform, and lots of holes can be founded on the surface. While a uniform composite membranous layer with TiN can be formed on the surface of the magnesium alloy by the multiple treatment technology, and the porosity of this layer is also reduced. And the multiply treated sample shows good anti-wear performance in dry sliding against the steel, the width of the wear trace is 227.5767 μm which is only 66% of the micro-arc oxidation one as the sliding distance reaches 175 m.

关键词： magnesium alloy micro-arc oxidation multiple treatment sliding wear

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Expression of tachyplesin gene in yeast Pichia pastoris

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Chinese science Bulletin 1998年第23期43卷 1986-1991页

作者： Chunyi Zhang Jun Zhao Changjian Wu Yunliu Fan Chinese acad agr sci Biotechnol Res Ctr Beijing 100081 Peoples R China

Tachyplesin gene was designed and chemically synthesized with codon usage bias. The stop codon TAG and some restriction sites convenient for further cloning were added to this gene. The synthesized gene cloned into yeast expression vector pPIC9 (with α_secretion signal) was transformed into host strain GS115 by electroporation. The tachyplesin expressed from recombinants Y PIC27 and Y PIC42 showed an inhibition activity against the germination of the spores of \%Magnaporthe grisea\%. Southern blot performed with chromosome genome of the two recombinants indicated a single copy of the expression cassette was integrated at the chromosomal AOX 1 locus by which the genomic AOX 1 gene was functionally disrupted and Northern blot showed the presence of transcripts of the tachyplesin gene.

关键词： tachyplesin gene expression \%Pichia pastoris.\%

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Exploration and analysis of the composition and mechanism of efficacy of camel milk

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FOOD BIOsciENCE 2023年第3期53卷 85-95页

作者： Liu, Chen Liu, Ling-Xiao Yang, Jie Liu, Yun-Guo zhejiang Univ Shandong Linyi Inst Modern Agr Linyi 276000 Peoples R China Linyi Univ Coll Life Sci Linyi 276005 Peoples R China Linyi acad agr sci Linyi 276005 Peoples R China Xinjiang Univ Coll Life Sci & Technol Urumqi 830046 Peoples R China

Camel milk is a high-quality milk with high nutritional value and health benefits. Camel milk contains a variety of nutrients such as fat, protein, vitamins, lactose, etc. The milk production and quality of camels is influenced by their size, age and feeding conditions. When camel milk is consumed, it is supplemented with nutrients, and after digestion and absorption in the body, it produces a large amount of angiotensin converting enzyme (ACE) inhibitory peptides and DPPV inhibitory peptides, which can also assist in the treatment of hypertension, dia-betes, immunity, delay kidney and liver damage, without toxic side effects to the body. It has also been found that the various biological activities of camel milk may be related to its exosomes. This review provides a comprehensive overview of the components of camel milk, its adjuvant therapeutic effects on diseases, and presents the high quality value of camel milk through the research literature.

关键词： Camel milk Nutrition Peptides Diabetes Hypertension

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Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt

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Chinese science Bulletin 1998年第18期43卷 1544-1550页

作者： Rongzhao Fu Yufa Peng Guangcheng Cao Jiangsheng Ma Caixia Chen Liming Zhang Wenbin Li Yongru Sun Chinese acad sci Genet Inst Beijing 100101 Peoples R China Chinese acad agr sci Inst Plant Protect Beijing 100094 Peoples R China

A cDNAderived 200 bp fragment encoding a rabbit defensin NP1 mature peptide was ligated into the GUSless pBI121 to yield pBIC35NP1. Tobacco leaf discs were genetically transformed with an agrobacterium tumefaciens strain harbouring pBIC35NP1. Northern blot analysis showed that the defensin NP1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum.

关键词： bacterial disease_resistance rabbit defensin tobacco transgenic plant.

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Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene

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Chinese science Bulletin 2002年第22期47卷 1871-1875页

作者： Lei Wang Jun Zhao Yunliu Fan Chinese acad agr sci Biotechnol Res Inst Beijing 100081 Peoples R China

A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.

关键词： maize ABRE ABP transcription factor function analysis.

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