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Isotope constraints on seasonal dynamics of nitrogen in zhanjiang Bay, a typical mariculture bay in South China

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Acta Oceanologica Sinica 2024年第6期43卷 60-70页

作者： Chunqing Chen Qibin Lao Fajin Chen Guangzhe Jin Jiacheng Li Qingmei Zhu College of Ocean and Meteorology Guangdong Ocean UniversityZhanjiang 524088China School of Chemistry and Environment Guangdong Ocean UniversityZhanjiang 524088China Key Laboratory for Coastal Ocean Variation and Disaster Prediction Guangdong Ocean UniversityZhanjiang 524088China Key Laboratory of Climate Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong ProvinceGuangdong Ocean UniversityZhanjiang 524088China

Eutrophication in coastal waters has been increasing remarkably,severely impacting the water quality in mariculture *** this study,we conducted multiple isotopic measurements on suspended particulate nitrogen(δ^(15)N-PN) and dissolved nitrate(δ^(15)N-NO_(3)^(-)and δ^(18)O-NO_(3)^(-)) in zhanjiang Bay,a typical mariculture bay with a high level of eutrophication in South China,to investigate the changes in nitrogen sources and their cycling between the rainy and dry *** the rainy season,the study found no significant relation between δ^(15)NPN and δ^(15)N-NO_(3)^(-)due to the impact of heavy rainfall and terrestrial *** the upper bay,a slight nitrate loss and slightly higher δ_(15)N-NO_(3)^(-)and δ^(18)O-NO_(3)^(-)values were observed,attributed to intense physical sedimentwater *** some fluctuations,nitrate concentrations in the lower bay mainly aligned with the theoretical mixing line during the rainy season,suggesting that nitrate was primarily influenced by terrestrial erosion and that nitrate isotopes resembled the ***,the isotopic values of nitrate can be used for source apportionment in the rainy *** results indicated that soil nitrogen(36%) and manure and sewage(33%) were the predominant nitrogen sources contributing to nitrogen loads during this *** contrast,the dry season saw a deficient ammonium concentration(zhanjiang B ay,providing valuable insights for formulati

关键词： nitrogen stable nitrogen isotopes biogeochemical processes eutrophication zhanjiang Bay

Effect of high-multiple water injection on rock pore structure and oil displacement efficiency

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Energy Geoscience 2024年第1期5卷 234-238页

作者： Xiao Lei Chunpeng Zhao Qiaoliang Zhang Panrong Wang Runfu Xiong zhanjiang branch Company CNOOCZhanjiang524057GuangdongChina SINOPEC Petroleum Exploration and Production Research Institute Beijing100083China

Experimental methods,including mercury pressure,nuclear magnetic resonance(NMR)and core(wateroil)displacement,are used to examine the effects of high-multiple water injection(*** injection with high injected pore volume)on rock properties,pore structure and oil displacement efficiency of an oilfield in the western South China *** results show an increase in the permeability of rocks along with particle migration,an increase in the pore volume and the average pore throat radius,and enhanced heterogeneity after high-multiple water *** with normal water injection methods,a high-multiple water injection is more effective in improving the oil displacement *** degree of recovery increases faster in the early stage due to the expansion of the swept area,and the transition from oil-wet to *** degree of recovery increases less in the late stage due to various factors,including the enhancement of heterogeneity in the *** both the economic aspect and the production limit of water flooding,it is recommended to adopt other technologies to further enhance oil recovery after 300 PV water injection.

关键词： High multiple Water injection Rock permeability Pore structure Oil displacement efficiency

Distribution and sources of sedimentary organic matter in different aquaculture areas of northeastern zhanjiang Bay using stable carbon and nitrogen isotopes

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Acta Oceanologica Sinica 2024年第6期43卷 38-48页

作者： Fengxia Zhou Shuangling Wang Han Fang Jiani He Liang Ye Zhaohai Ding Cuiting Li Fajin Chen Xuan Lu Chunqing Chen Yafei Meng College of Ocean and Meteorology Guangdong Ocean UniversityZhanjiang 524088China Key Laboratory for Coastal Ocean Variation and Disaster Prediction Guangdong Ocean UniversityZhanjiang 524088China Key Laboratory of Climate Resources and Environment in Continental Shelf Sea and Deep Sea of Department of Education of Guangdong ProvinceGuangdong Ocean UniversityZhanjiang 524088China

zhanjiang Bay is a major aquaculture area in China with many types of mariculture products(such as oysters,fish,and shrimp).The culture area and shrimp output in zhanjiang Bay are ranked first in *** investigated the total organic carbon(TOC),total nitrogen(TN),TOC/TN ratio,and stable isotopes(δ^(13)C and δ^(15)N) of the fish and shrimp feed,fish and shrimp feces,and sedimentary organic matter(SOM) in and around different aquaculture areas of northeastern zhanjiang B ay to study the impact of aquaculture activities on *** average TOC contents of fish and shrimp feed were 39.20%±0.91% and 39.29%±0.21%,*** average TOC content in the surface sediments of the oyster culture area,the mixed(fish and shrimp) culture area,and the cage fish farm area were 0.66%,0.88%±0.10%,and 0.58%±0.19%,respectively,which may indicate that mixed culture had a greater impact on *** relatively high TOC and TN contents and relatively low TOC/TN ratios,and δ^(15)N values in the upper layer of the core sediment in the mixed culture area could also support the significant influence of mixed *** average δ^(13)C and δ^(15)N values of fish and shrimp feed were -20.6‰±2.2‰ and 1.8‰±1.2‰,respectively,which were different from the isotopic values of SOM in the study area.δ^(13)C and δ^(15)N values for SOM in different aquaculture areas were different from those of nearby reference stations,probably reflecting the influence of *** δ^(13)C and δ^(15)N values in the oyster culture area(-25.9‰ and6.0‰,respectively) seemed to have reduced δ^(13)C and enriched δ^(15)N relative to those of the reference station(-24.6‰ and 5.8‰,respectively).This may reflect the influence of organic matter on oyster *** δ^(15)N value of the station in the mixed culture area(7.1‰±0.4‰) seemed to be relatively enriched in δ^(15)N relative to that of the reference station(6.6‰).Sedimentation and the subsequent degradation of organic matter from mixed cultures may have contrib

关键词： sedimentary organic matter aquaculture stable isotopes sources zhanjiang Bay

New insights into the DPR mechanism of elastic energy released by polymer gel for enhanced oil recovery

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Petroleum 2024年第3期10卷 539-547页

作者： Hu Jia Junyi Wu Shaowei Wu Yanbin Liang Minghao Wang Xiaojin Wan Pengwu Li State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduChina CNOOC zhanjiang branch ZhanjiangChina Oil Production Technology Institute of PetroChina Dagang Oilfield Company TianjinChina

Polymer gel was widely used as water shutoff agent in mature oil *** the results of single-phase plugging experiments show that the plugging rate of the polymer gel to the oil phase is lower than that of the water ***,the disproportionate permeability reduction(DPR)mechanism of polymer gels still remains *** this paper,we used four gel formulations including polyethyleneimine(PEI)and phenol-formaldehyde crosslinked gel with and without adding laponite to investigate the effect of gel elastic property on the water shutoff *** result of sand pack flooding experiments shown that the gel with higher elastic modulus has better effects on decreasing water cut and increasing oil *** adding laponite,the elastic modulus of phenol-formaldehyde crosslinked gel increased from 64.2 Pa to 192 Pa,and the elastic modulus of PEI crosslinked gel increased from 27.4 Pa to 36.5 *** to the phenol-formaldehyde-HAPM gel,the oil recovery of laponite-phenol-formaldehyde-HPAM gel increased by 5.2%and the maximum water cut decreased by 8.3%.Besides,comparing with PEI-HPAM gel,the oil recovery of laponite-PEI-HPAM gel increased by 2.7%and the water cut dropped by 27.8%.In the meanwhile,the laponite-phenol-formaldehyde-HPAM gel with higher elastic modulus obviously swells in the formation water but almost remains constant in oil at 105℃.The mass of gel soaked in the formation water increased from 42 g to 96 g and the gel volume increased by 300%within 48 *** study improves the understanding of the DPR mechanism of polymer gel for water shutoff.

关键词： Gel elasticity Water shutoff DPR Laponite Oil recovery

Monitoring Technologies for Marine Carbon Sequestration in zhanjiang

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哈尔滨工程大学学报（英文版） 2024年第3期23卷 617-627页

作者： Yiwen Xiong Yangze Dong Xiahua Chen Wenchang Ling zhanjiang Bay Laboratory Zhanjiang 524000China

Marine carbon sequestration is an important component of carbon dioxide capture, utilization and storage(CCUS) technology. It is crucial for achieving carbon peaking and carbon neutralization in China. However, CO_(2) leakage may lead to seabed geological disasters and threaten the safety of marine engineering. Therefore, it is of great significance to study the safety monitoring technology of marine carbon *** is industrially developed and rich in carbon sources. Owing to the good physical properties and reservoirs and trap characteristics,zhanjiang has huge storage potential. This paper explores the disaster mechanism associated with CO_(2) leakage in marine carbon sequestration areas. Based on the analysis of the development of zhanjiang industry and relevant domestic monitoring technologies, several suggestions for safety monitoring of marine carbon sequestration are proposed: application of offshore aquaculture platforms, expansion and application of ocean observation networks, carbon sequestration safety monitoring and sensing system. Intended to build a comprehensive and multi-level safety monitoring system for marine carbon sequestration, the outcome of this study provides assistance for the development of marine carbon sequestration in China's offshore areas.

关键词： Marine carbon sequestration Carbon dioxide capture Utilization and storage(CCUS) CO_(2)leakage Monitoring technologies

Combined intestinal microbiota and transcriptomic analysis to investigate the effect of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana

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Animal Nutrition 2024年第3期18卷 203-219页

作者： Hang Yuan Minghua Xie Jian Chen Naijie Hu Honming Wang Beiping Tan Lili Shi Shuang Zhang College of Fisheries Guangdong Ocean UniversityZhanjiangChina College of Fisheries and Life Science Dalian Ocean UniversityDalianChina zhanjiang Experimental Station Chinese Academy of Tropical Agricultural SciencesZhanjiangChina

Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana(CHL),a 3×2 factorial design stocking experiment was used in this ***,shrimp was fed with two dietary protein sources(fishmeal[FM]and CHL)at low(LSD;100 per m^(3)),medium(MSD;200 per m^(3))and high(HSD;300 per m^(3))stocking densities for 8 *** growth performance and resistance to Vibrio parahaemolyticus(1.0×10^(7) CFU/mL)of shrimp decreased with the increase of stocking density,but dietary CHL improved this *** between the CHL and FM groups for *** resistance were significant only under high-density conditions(P<0.05).Significant interactions between stocking density and protein source were found on the activities of catalase(CAT),superoxide dismutase(SOD)and phenol oxidase(PO),and the contents of malondialdehyde(MDA)in the hepatopancreas and the activities of intestinal amylase,most of which were significantly different between CHL and FM groups only at high stocking density(P<0.05).Analysis of 16S rDNA sequencing showed that dietary CHL increased the alpha diversity of intestinal microbiota,inhibited the colonization of pathogenic bacteria and enhanced the abundance of beneficial *** results showed that at high stocking densities,differentially expressed genes(DEGs)in the FM vs CHL group were mostly upregulated and primarily enriched in immune and metabolic related pathways including Toll,immune deficiency(Imd)and glycolysis–gluconeogenesis *** correlation analysis revealed significant correlation between the top ten intestinal bacteria at the genus level and markedly enriched DEGs,also more were detected under high density *** conclusion,CHL has great potential as a novel protein source in the intensive farming of shrimp.

关键词： Chlorella sorokiniana Stocking density Microbiota structure Transcriptomic profile Litopenaeus vannamei

Characteristics of transfer zones under the influence of preexisting faults and regional stress transformation:Wenchang A subsag,Zhujiang River Mouth Basin,northern South China Sea

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Journal of Oceanology and Limnology 2024年第3期42卷 744-759页

作者： Wei LI Mingyue CAO Meifang MENG Caiwei FAN Hui LI Jia LI Wanqiu WU Ruiqing QIN Xingan LI Yiming LIU State Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao 266580China School of Geosciences China University of Petroleum(East China)Qingdao 266580China zhanjiang branch China National Offshore Oil Corporation CompanyZhanjiang 524057China

A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon *** on the systematic analysis of 3D seismic data and hydrocarbon accumulation conditions,the geometry,kinematics,and reservoir control of a large synthetic overlapping transfer zone in the south of the Wenchang A subsag in the Zhujiang(Pearl)River Mouth basin were *** indicate that the development and evolution of the transfer zone was controlled by the interaction between pre-existing faults and regional stress *** intense rifting of the main faults of the transfer zone controlled the development of source rocks and faultcontrolled slope break *** strike-slip overprint since the Oligocene is conducive to the formation of a large-scale fault-anticline trap,and the secondary faults in the transfer zone contribute to the hydrocarbon *** conjugate intersection area of the NE-and NW-trending faults offers more opportunity for hydrocarbon migration and accumulation.

关键词： transfer zone pre-existing fault regional stress transformation Cenozoic Wenchang A Subsag Zhujiang(Pearl)River Mouth Basin

Identification of Shell Color-Associated Genes Using Mantle branch-Specific RNA Sequencing of Yellow-Colored Line of Pearl Oyster Pinctada fucata martensii

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Journal of Ocean University of China 2024年第4期23卷 1076-1086页

作者： PENG Haiming LIAO Yongshan YANG Chuangye MKUYE Robert DENG Yuewen YUE Chenyang Fisheries College Guangdong Ocean UniversityZhanjiang 524088China Pearl Research Institute Guangdong Ocean UniversityZhanjiang 524088China Guangdong Science and Innovation Center for Pearl Culture Zhanjiang 524088China Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province Zhanjiang 524088China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture Zhanjiang 524088China

The yellow-colored line of pearl oyster Pinctada fucata martensii displays a yellow prismatic layer and a white nacreous layer that can be used as an ideal model for research on shell color ***-Raman spectroscopy and transcriptome analyses were performed to explore the potential molecular mechanism underlying the phenotype *** micro-Raman spectroscopy results indicate that the prismatic layer exhibits distinct characteristic peaks of carotenoids,while these peaks are not prominent in the nacreous *** the transcriptome comparison of the central zone of mantle and mantle edge tissue,which function in nacreous and prismatic layer formation,respectively,935 significantly differentially expressed genes(DEGs)were identified,with 385 genes upregulated and 227 genes downregulated(|log_(2)(Fold change)|>1 and false discovery rate<0.05)in the mantle edge *** these genes,some were associated with melanoma/melanogenesis,such as tyrosinase,zinc metalloprotease,glutathione S-transferase,and ATP-binding cassette sub-family;some were associated with the carotenoid-related pathway,including scavenger receptors,cytochrome P450 and lipoprotein *** associated with porphyrin metabolism,including porphobilinogen deaminase,and copper/zinc superoxide dismutase,and genes associated with shell matrix protein,including amorphous calcium carbonate binding protein,shematrin,PIF,and collagen,also exhibited significantly different *** is speculated that the different colours between prismatic layer and nacreous layer in the yellow-colored line of *** might be resulted from melanin,carotenoids and porphyrin metabolism,while genes related to shell structure and biomineralization might also affect *** results provide new insights to understand the mechanism of shell color formation in mollusca.

关键词： Pinctada fucata martensii shell coloration transcriptome

Experimental study of the effects of a multistage pore-throat structure on the seepage characteristics of sandstones in the Beibuwan Basin:Insights into the flooding mode

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Petroleum Science 2024年第2期21卷 1044-1061页

作者： Lei Wang Xiao Lei Qiao-Liang Zhang Guang-Qing Yao Bo Sui Xiao-Jun Chen Ming-Wei Wang Zhen-Yu Zhou Pan-Rong Wang Xiao-Dong Peng zhanjiang branch China National Offshore Oil Corporation(CNOOC)LimitedZhanjiang524057GuangdongChina Faculty of Earth Resources China University of Geosciences(Wuhan)Wuhan430074HubeiChina School of Resources and Safety Engineering Chongqing UniversityChongqing400044China School of Engineering University of ManchesterManchester M139WJUK Hainan branch China National Offshore Oil Corporation(CNOOC)LimitedHaikou570311HainanChina

To investigate the relationship between grain sizes, seepage capacity, and oil-displacement efficiency in the Liushagang Formation of the Beibuwan Basin, this study identifies the multistage pore-throat structure as a crucial factor through a comparison of oil displacement in microscopic pore-throat experiments. The two-phase flow evaluation method based on the Li-Horne model is utilized to effectively characterize and quantify the seepage characteristics of different reservoirs, closely relating them to the distribution of microscopic pores and throats. It is observed that conglomerate sandstones at different stages exhibit significant heterogeneity and noticeable differences in seepage capacity, highlighting the crucial role played by certain large pore throats in determining seepage capacity and oil displacement efficiency. Furthermore, it was found that the displacement effects of conglomeratic sandstones with strong heterogeneity were inferior to those of conventional homogeneous sandstone, as evidenced by multiple displacement experiments conducted on core samples with varying granularities and flooding systems. Subsequently, core-based experiments on associated gas flooding after water flooding were conducted to address the challenge of achieving satisfactory results in a single displacement mode for reservoirs with significant heterogeneity. The results indicate that the oil recovery rates for associated gas flooding after water flooding increased by 7.3%-16.4% compared with water flooding alone at a gas-oil ratio of approximately 7000 m^(3)/m^(3). Therefore, considering the advantages of gas flooding in terms of seepage capacity, oil exchange ratio, and the potential for two-phase production, gas flooding is recommended as an energy supplement mode for homogeneous reservoirs in the presence of sufficient gas source and appropriate tectonic angle. On the other hand, associated gas flooding after water flooding is suggested to achieve a more favorable develop

关键词： Beibuwan Basin Multistage pore-throat structure Multistage seepage characteristics Microscopic visualization Heterogeneity Gas/water flooding Flooding mode