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A dual-protease-triggered chemiluminescent probe for precise tumor imaging

Chinese Chemical Letters 2025年第2期36卷 302-306页

作者： Zhihui Zhang Ru Sun Chong Bian Hongbo Wang Zhen Zhao Panpan Lv Jianzhong Lu Haixin Zhang Hulie Zeng Yuanyuan Chen Zhijuan Cao Department of Pharmaceutical Analysis School of PharmacyFudan University Minhang Branch Zhongshan HospitalFudan University China State Institute of Pharmaceutical industry Shanghai Jahwa United co. Ltd.

Imaging detection of interlinked dual proteases is imperative for precise tumor imaging, which remains challenging due to limited modification position of specific substrate and possible steric ***, we have developed a unimolecular chemiluminescent probe(LGP-CL) tandemly activated by two proteases interlinked with liver cancer to achieve precise tumor imaging. Probe LGP-CL co.sists of a phenoxy-dioxetane scaffold caged by a tripeptide substrate(LGP, leucine-glycine-proline) as the sensing layer, which can be cleaved sequentially by aminopeptidase N(APN) and dipeptidyl peptidase IV(DPPIV)to turn on a strong chemiluminescent signal, and silenced by specific inhibitor of each enzyme, which acco.nts for an integrated logic gate(AND, OR and INHIBIT). The successful cleavage of dual proteases on the metabolic site depends on the proper structure of the tripeptide substrate, as co.firmed by two probes design. Probe LGP-CL(LGP as the substrate) enables the excellent “dual-lock-dual-key” fit with a382-fold enhancement of chemiluminescent emission while no obvious signal is observed by using GPLCL(GPL as the substrate). By virtue of its rapid response(several minutes), high sensitivity and good cell viability, probe LGP-CL has been utilized to evaluate upregulated levels of proteases in vitro and in living systems, especially to distinguish liver tumor cells(Hep G2) from others(LO2, MCF-7, MCF-10a and RAW264.7). Overall, the newly developed CL probe may facilitate rapid investigation into the role played by proteases in liver diseases, enabling timely selection appropriate treatment. Therefore, our work not only sheds light on the rational design of optical probes for dual protease imaging, but provides a promising tool for clinical diagnosis and even drug disco.ery.

关键词： Chemiluminescent Dual-protease imaging Logic gates Phenoxy-dioxetane scaffold Tripeptide substrate

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Fine mapping and identification of CaTTG1,a candidate gene that regulates the hypoco.yl anthocyanin accumulation in Capsicum annuum

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Horticultural Plant Journal 2025年第1期11卷 264-274页

作者： Jin Wang Yao Dai Luzhao Pan Ying Chen Li Dai Yanqing Ma Xiaoxun Zhou Wu Miao Muhammad Rizwan Hamid Xuexiao Zou Feng Liu Cheng Xiong Engineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops Key Laboratory for Vegetable Biology of Hunan ProvinceCollege of HorticultureHunan Agricultural UniversityChangshaHunan 410000China Hunan Xiangyan Seed industry co. LTDChangshaHunan 410000China

Pepper(Capsicum annuum L.)is a typical self-pollinating crop with obvious heterosis in ***,the use of morphological markers during the pepper seedling stage is crucial for pepper *** co.or of hypoco.yl is widely used as a phenotypic marker in crossing studies of *** accessions generally have purple hypoco.yls,which are mainly due to the anthocyanin accumulation in seedlings,and green hypoco.yls are rarely observed in *** we reported the characterization of a green hypoco.yl mutant of pepper,Cha1,which was identified from a pepper ethyl methanesulfonate(EMS)mutant *** mapping revealed that the causal gene,CaTTG1,belonging to the WD40 repeat family,co.trolled the green hypoco.yl phenotype of the ***-induced gene silencing(VIGS)co.firmed that CaTTG1 regulated anthocyanin ***-seq data showed that expression of structural genes CaDFR,CaANS,and CaUF3GT in the anthocyanin biosynthetic pathway was significantly decreased in Cha1 co.pared to the wild *** two-hybrid(Y2H)experiments also co.firmed that CaTTG1 activated the synthesis of anthocyanin structural genes by forming a MBW co.plex with CaAN1 and *** summary,this study provided a green hypoco.yl mutant of pepper,and the Kompetitive Allele Specific PCR(KASP)marker developed based on the mutation site of the underlying gene would be helpful for pepper breeding.

关键词： Pepper CaTTG1 Anthocyanin Hypoco.yl co.or Fine-mapping

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Microstructure and Wear/co.rosion Resistance of Stainless Steel Laser-alloyed with Mn+W_(2)C, Mn+NiWC and Mn+SiC

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 283-294页

作者： ZHOU Rui DIAO Xiaogang SUN Yixin School of Metallurgical Engineering Jiangsu University of Science and TechnologyZhangjiagang 215600China CITIC Heavy Cast Iron industry co. Ltd.Luoyang 417039China

In-situ formed high Mn steel co.ting reinforced by carbides was formed by laser surface alloying(LSA).Laser alloyed layers on 1Cr18Ni9Ti steel with Mn+W_(2)C(specimen A),Mn+NiWC(specimen B)and Mn+SiC(specimen C)powders were fabricated to improve the wear and co.rosion behavior of 1Cr18Ni9Ti steel blades in high speed *** evolution,phases,element distribution,microhardness,wear and co.rosion behavior of the laser alloyed layers were *** indicated that high Mn steel matrix co.posites with undissolved W_(2)C,WC and other in-situ formed carbides were formed by LSA with Mn+W_(2)C and Mn+NiWC while SiC totally dissolved into the high Mn matrix when adding Mn+*** as the binding phase in Ni-WC powder decreased the crack sensitivity of the alloyed layer as co.pared with the addition of W_(2)C *** improvement in average microhardness was achieved in the matrix in specimen A,B and C,with the value of 615,602 and 277 HV_(0.5),while that of the substrate was 212 HV_(0.5).The increase of microhardness,wear and co.rosion resistance is highly co.elated to microstructure,formed phases,type and co.tent of carbides,micro-hardness and toughness of the alloyed layers.

关键词： laser surface alloying stainless steel carbide type microstructure wear and co.rosion resistance

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Vibration safety assessment and parameter analysis of buried oil pipelines based on vibration isolation holes under strong surface impact

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Earthquake Engineering and Engineering Vibration 2025年第1期24卷 69-82页

作者： Wang Guobo Mei Hua Wang Jianning He Wei Yin Yao Zhai Yuxin Zuo Pengfei co.lege of Civil Engineering and Architecture Wenzhou UniversityWenzhou 325035China Zhejiang Engineering Research Center of Disaster Prevention and Mitigation for co.stal Soft Soil Foundation WenZhou 325035China China National Machinery industry co. Ltd.Beijing 100080China Faculty of Engineering China University of GeosciencesWuhan 430074China China Railway co.struction Group co. Ltd.Beijing 100072China School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan 430070China

Strong surface impact will produce strong vibration,which will pose a threat to the safety of nearby buried pipelines and other important lifeline *** on the verified numerical method,a co.prehensive numerical parameter analysis is co.ducted on the key influencing factors of the vibration isolation hole(VIH),which include hole diameter,hole net spacing,hole depth,hole number,hole arrangement,and soil *** results indicate that a smaller ratio of net spacing to hole diameter,the deeper the hole,the multi-row hole,the hole adoption of staggered arrangements,and better site soil co.ditions can enhance the efficiency of the VIH *** average maximum vibration reduction efficiency within the vibration isolation area can reach 42.2%.The vibration safety of adjacent oil pipelines during a dynamic co.paction projection was evaluated acco.ding to existing standards,and the measurement of the VIH was reco.mended to reduce excessive *** single-row vibration isolation scheme and three-row staggered arrangement with the same hole parameters are suggested acco.ding to different *** research findings can serve as a reference for the vibration safety analysis,assessment,and co.trol of adjacent underground facilities under the influence of strong surface impact loads.

关键词： vibration isolation hole buried oil pipeline strong surface impact vibration velocity vibration safety assessment

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Preparation of sodium molybdate from molybdenum co.centrate by microwave roasting and alkali leaching

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International Journal of Minerals,Metallurgy and Materials 2024年第1期31卷 91-105页

作者： Fengjuan Zhang Chenhui Liu Srinivasakannan Chandrasekar Yingwei Li Fuchang Xu School of Chemistry and Environment Yunnan Minzu UniversityKunming 650500China Chemical Engineering Department Khalifa UniversityAbu DhabiUAE yunnan Zhong Tai Technology co. Ltd.Kunming 650500China yunnan yongchang silicon industry co. Ltd.Baoshan 678000China

The preparation process of sodium molybdate has the disadvantages of high energy co.sumption,low thermal efficiency,and high raw material requirement of molybdenum trioxide,in order to realize the green and efficient development of molybdenum co.centrate resources,this paper proposes a new process for efficient reco.ery of molybdenum from molybdenum co.centrate and preparation of sodium molybdate by microwave-enhanced roasting and alkali *** analysis indicated the feasibility of oxidation roasting of molybdenum *** effects of roasting temperature,holding time,and power-to-mass ratio on the oxidation product and leaching product sodium molybdate (Na_(2)MoO_(4)·2H_(2)O) were *** the optimal process co.ditions:roasting temperature of 700℃,holding time of 110 min,and power-to-mass ratio of 110 W/g,the molybdenum state of existence was co.verted from MoS_(2) to Mo *** process of preparing sodium molybdate by alkali leaching of molybdenum calcine was investigated,the optimal leaching co.ditions include a solution co.centration of 2.5 mol/L,a liquid-to-solid ratio of 2 mL/g,a leaching temperature of 60℃,and leaching solution termination at pH *** optimum co.ditions result in a leaching rate of sodium molybdate of 96.24%.Meanwhile,the co.tent of sodium molybdate reaches 94.08wt%after leaching and removing *** and aluminum impurities can be effectively separated by adjusting the pH of the leaching solution with sodium carbonate *** research avoids the shortco.ings of the traditional process and utilizes the advantages of microwave metallurgy to prepare high-quality sodium molybdate,which provides a new idea for the highvalue utilization of molybdenum co.centrate.

关键词： molybdenum metallurgy microwave oxidation roasting removing impurities sodium hydroxide leaching

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CRISPR-Cas12a co.pled with Universal Dual-mode Fluorescent Nanoparticles Platform for HPV Nucleic Acid Detection

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Journal of Analysis and Testing 2025年第01期 153-159页

作者： Li Xu Sitong Liu Zhaohe Huang Xiaojing Pei Shujing Li Yifan He Yigang Tong Guoqi Liu School of Light industry Science and Engineering Beijing Technology and Business University co.lege of Life Science and Technology Beijing University of Chemical Technology Biotecnovo(Beijing) co. LtdBeijing Economic and Technological Development Zone

Cervical cancer is a prevalent gyneco.ogical malignancy, with approximately 90% of cases attributed to human papillomavirus(HPV) infection. Rapid and accurate nucleic acid detection is one of the leading methods to improving screening co.erage for early cervical cancer diagnosis. However, most existing techniques are usually co.plex and require expensive instrumentation. Clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated systems have great advantages in nucleic acid detection. We herein co.bined the CRISPR-Cas12a with a universal dual-mode fluorescent nanoparticles(FNPs) platform to co.struct a highly sensitive signal-offassay for HPV high-risk subtypes detection. The signal readout module uses a single-stranded DNA linker, which forms a sandwich structure with DNA-functionalized magnetic beads and DNA-functionalized FNPs to generate signals. If trans-cleavage activity was activated by the targets, the linker was co.sumed and therefore co.ld not form the sandwich structure to produce signals. HPV16 and HPV18 as model targets, the limits of detection as low as 5 pmol/L were successfully achieved without amplification. We validated the feasibility of the real-sample detection using HPV16 and HPV18 pseudo viruses. The proposed method can be easily adapted for other virus or bacterial assays by modifying the CRISPR-derived RNA(crRNA), which shows great potential for clinical diagnosis.

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Polysaccharide from large leaf yellow tea activates the LXRα-ABCA1/ABCG1 pathway to ameliorate type 2 diabetes by regulating gut microbiota

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Food Science and Human Wellness 2025年

作者： Hao Chen Zhuang Wang Xinxin Pei Jielin Chen Kaili Xuan Lei Gong Xinyu Zhou Yuzhe Huang Yan Chen School of Life Sciences， Anhui University Key Laboratory of Eco.ogical Engineering and Biotechnology of Anhui Province Institutes of Physical Science and Information Technology， Anhui University Fugong Yuanlveda Puluo Tea industry co.， Ltd.

Type 2 diabetes mellitus （T2DM） is a multifaceted metabolic co.dition characterized by chronic hyperglycemia （elevated blood sugar levels） and insulin resistance （IR）. We have isolated a new homogeneous polysaccharide， LYTP-3， (7.22 × 105Da) with anti-diabetic activity， from large leaf yellow tea （LYT） by biological activity-guided sequential separation and activity evaluation. Using a T2DM mouse model， we demonstrate that oral administration of LYTP-3 can reduce blood lipid levels， alleviate hyperglycemia and gluco.e tolerance， and inhibit insulin resistance. LYTP-3 ameliorated T2DM treatment by activating the liver X receptor alpha （LXRα）-ATP-binding cassette （ABC） transporter （LXRα-ABCA1/ABCG1） signaling pathway， which is regulated by the gut microbiota. The strain Lachnospiraceae bacterium A4 is potentially the key to this improvement. Additionally， we have solved the molecular structure of LYTP-3 using nuclear magnetic resonance （NMR）， and methylation analyses. The main backbone of LYTP-3 co.sists of 1，2，4-α-L-Rhap， 1，4-α-D-Galp， 1，6-α-D-Glcp， with branches of 1，4-β-D-Galp， 1，6-β-D-GalpA， 1，3，6-β-D-Manp， 1，2-α-L-Rhap， T-β-Manp， and T-β-Galp. The findings of this study suggest that LYTP-3 may serve as a potential gut microbiota modulator in the treatment of T2DM.

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Boosting Photocatalytic Performance of Cu-Mn co.doped CeO_(2) Nanoparticles for Tetracycline Degradation

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 42-48页

作者： XU Rongqi MI Jianxin WANG Shulin XU Man ZHU Li BAI Lei TANG Dandan LEI Yu Engineering Research Center of Environmental Materials and Membrane Technology of Hubei Province School of Materials Science and EngineeringWuhan Institute of TechnologyWuhan 430205China Shaanxi co.l Chemical industry Technology Research Institute co. Ltd.Xi’an 710100China

Cu-Mn co.doped CeO_(2) photocatalyst was successfully synthesized by the sol-gel method to assess its capability in degrading *** and TEM results showed that Cu and Mn were successfully co.doped into CeO_(2) without forming heterostructure,XPS and photoelectrochemical results revealed that Mn ions doping amplified the generation of photo-induced charge carriers,while Cu ions doping significantly facilitated the interfacial charge transfer ***,the optimized Cu3Mn2CeO_(2) nanoparticles exhibited the highest TC removal efficiency,achieved a rate of 78.18%and maintained a stable cycling performance.

关键词： photocatalyst co.doped charge separation water treatment

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Multi-interface structure design of bamboo-based carbon/co.co. co.posite electromagnetic wave absorber based on biomimetic honeyco.b-shaped superstructure

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International Journal of Minerals,Metallurgy and Materials 2025年第3期32卷 631-644页

作者： Yanting Wang He Han Huiyang Bian Yanjun Li Zhichao Lou Jiangsu co.Innovation Center of Efficient Processing and Utilization of Forest Resources College of Materials Science and EngineeringNanjing Forestry UniversityNanjing 210037China co.lege of Light industry and Food Engineering Nanjing Forestry UniversityNanjing 210037China

The rapid development of 5G co.munication technology and smart electronic and electrical equipment will inevitably lead to electromagnetic radiation *** heterointerface polarization relaxation through nanostructure design and interface modifica-tion has proven to be an effective strategy to obtain efficient electromagnetic wave ***,this work implements an innovative method that co.bines biomimetic honeyco.b superstructure to co.strain hierarchical porous heterostructure co.posed of co.co. nano-particles to improve the interfacial polarization *** method effectively co.trolled the absorption efficiency of co.(2+)through de-lignification modification of bamboo,and co.bined with the bionic carbon-based natural hierarchical porous structure to achieve uniform dispersion of nanoparticles,which is co.ducive to the in-depth co.struction of heterogeneous *** addition,the multiphase struc-ture brought about by high-temperature pyrolysis provides the best dielectric loss and impedance matching for the ***,the obtained bamboo-based co.co. multiphase co.posite showed excellent electromagnetic wave absorption performance,achieving excel-lent reflection loss(RL)of-79 dB and effective absorption band width of 4.12 GHz(6.84-10.96 GHz)at low load of 15wt%.Among them,the material’s optimal radar cross-section(RCS)reduction value can reach 31.9 dB·m^(2).This work provides a new approach to the micro-co.trol and co.prehensive optimization of macro-design of microwave absorbers,and offers new ideas for the high-value utiliza-tion of biomass materials.

关键词： biomass honeyco.b porous heterojunction structure interfacial polarization electromagnetic wave absorption

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Emerging organic carbonyl small molecules cathode materials in alkali-ion batteries: Principles, recent progresses, strategies and prospects

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Progress in Natural Science:Materials International 2025年第01期 31-50页

作者： Suping Chen Xijun Xu Tao Yang Weizhen Fan Jingwei Zhao Yanping Huo School of Chemical Engineering and Light industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Research and Development Center Guangzhou Tinci Materials Technology Co. Ltd. Analytical&Testing Center Guangdong University of Technology

Carbonyl small molecule co.pounds hold great potential as cathode materials for alkali-ion batteries（AIBs） due to their environmental friendliness, structural designability, and high theoretical capacity. However, it's practical application is hindered by intrinsic drawbacks, such as low electrical co.ductivity and high solubility in organic electrolytes. Additionally, most carbonyl small molecule cathodes can't be charged first in half-cells due to the absence of detachable Li+/Na+/K+ions, diminishing their appeal when assembled into full cells with alkali-free anodes. This review provides a detailed overview of carbonyl-co.tained small molecule cathode materials centered around oxygen, including their charge storage mechanisms, solutions to overco.e their drawbacks, and the latest research advancements in AIBs. This review also summarizes the electrochemical performance of carbonyl small molecules as cathodes that have been assembled in batteries. Finally, the review outlines the prospects and directions for future research on carbonyl small molecule cathodes. It is hoped that this review will offer meaningful guidance for the development of carbonyl small-molecule cathode materials in AIBs.

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