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Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang Yuying Bai Xia Hu Jinlian Chen Jun Sheng Yang Tian College of food Science and Technology Yunnan Agricultural UniversityKunming 650201China yunnan Provincial key laboratory of precision nutrition and personalized food manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional food Yunnan Agricultural UniversityKunming 650201China key laboratory of Pu-er Tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain *** aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro *** total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were *** kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive *** is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active *** results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

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Exploring the material basis and mechanism of Moringa oleifera in alleviating slow transit constipation based on network pharmacology and animal models

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food Science and Human Wellness 2024年

作者： Sijin Li Xiaoyu Gao Kaifeng Guo Shuangfeng Liu Weiqian Yang Yan Zhao Jun Sheng Zhongbin Bai Yang Tian College of food Science and Technology， yunnan Agricultural University yunnan key laboratory of precision nutrition and personalized food manufacturing， yunnan Agricultural University Department of Science and Technology， yunnan Agricultural University College of Veterinary Medicine， yunnan Agricultural University Engineering Research Center of Development and Utilization of food and Drug Homologous Resources， Ministry of Education， yunnan Agricultural University

Moringa oleifera(M. oleifera) have laxative effects， but their active compositions and mechanisms are not very clear thus far. To this end， we systematically explored the active components and mechanism of M. oleifera leaves in relieving constipation by using the slow transit constipation (STC) mouse model and network pharmacology. The results of animal experiments showed that M. oleifera aqueous extract (MOA) had good laxative activity， and its 70% alcohol soluble part (ASP) also showed significant laxative activity(P key compound of ASP， and it might relieve constipation through tachykinin receptor 1 (TACR1) and three kinds of adrenergic receptors， including alpha-1a (ADRA1A)， alpha-2a (ADRA2A)， and alpha-2b (ADRA2B). Further animal experiment results showed that Phe significantly promoted gastrointestinal motility. Phe may relieve STC by enhancing the release of substance P (SP) and upregulating the mRNA expression of TACR1 in the ileum. Importantly， Phe may also promote intestinal movement by downregulating ADRA2A and ADRA2B and upregulating calmodulin and the mRNA and protein expression of myosin light chain 9 (MYL9) in the ileum， thereby activating the GPCR-MLC signaling pathway. These results lay a foundation for the application of M. oleifera and Phe in constipation.

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Moringia oleifera Lam. seeds isothiocyanate against skin photoaging by regulating p38MAPK-dependent autophagy signalling pathway

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food Science and Human Wellness 2023年

作者： Min Yang Ling-Yan Su Liang Tao Ling-Fei Li Yi-Jin Wang Zi-Lin Wang Cun-Chao Zhao QianQian-OuYang Jun Sheng Yang Tian College of food Science and Technology， yunnan Agricultural University yunnan key laboratory of precision nutrition and personalized food manufacturing， yunnan Agricultural University PuEr University Marine Biomedical Research Institution， Guangdong Medical University Engineering Research Center of Development and Utilization of food and Drug Homologous Resources， Ministry of Education， yunnan Agricultural University The Marine Biomedical Research Institute of Guangdong Zhanjiang， China

People living long-term in areas with UV will cause premature photoaging. An abnormal reduction in autophagy is a key feature of photoaging， and p38MAPK has been regarded as a key regulator of autophagy. Isothiocyanate is one of the main active components of Moringa oleifera Lam. seeds. Studies have reported that MITC has anticancer， anti-inflammatory， cardiometabolic repair， nervous system protection， blood lipid regulation and diabetes prevention properties. However， the molecular mechanisms of MITC with protective effects against skin photoaging have not been studied thus far. In this study， we aimed to evaluate the antiphotoaging activity of MITC and to investigate the effect of p38MAPK-dependent autophagy in vivo and in vitro models of photoaging. In this research we found that MITC can reverse the intracellular ROS content and inhibit the activation of p38MAPK to improve the autophagy level， reduce the expression of MMPs， and finally protect against photoaging by UV. Our results will uncover the molecular mechanisms of MITC that play a role in the protective effects against skin photoaging， provide helpful information for developing MITC as an ant-photoaging plant material and improve the utilization of Moringa oleifera Lam. seeds.

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