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Establishment and Application of Prevention and co.trol Techniques of New Sugarcane White Leaf Disease

agricultural Biotechnology 2024年第4期13卷 18-20,27页

作者： Xiaoyan WANG Ruonan QIU Hong XU Yulong JIANG Rongyue ZHANG Fengyun ZHANG Ming GONG Jinxue LEI Hongli SHAN Jie LI Yingkun HUANG Key Laboratory on Genetic Improvement of Sugarcane in yunnan Province Sugarcane Research InstituteYunnan Academy of Agricultural SciencesKaiyuan 661699China Eco.omic Crop Workstation Lancang Lahu Autonomous CountyLancang 665600China yunnan Zichen agricultural Development co. Ltd.Xiangyun 672100China

Aiming at the basic and key technical problems in prevention and co.trol of sugarcane white leaf disease(SCWL),this study systematically overcame key technical bottleneck of prevention and co.trol of new SCWL after 10 years of co.laborative research,and achieved several innovative *** phytoplasmas newly reco.ded in China and the new subgroup of SCWL phytoplasmas(16SrXI-D)were disco.ered for the first time in yunnan,and the whole genome analysis of the epidemic subgroup was *** main transmission source of SCWL pathogens has been identified as infected seed canes,and Tettigoniella viridis and Clovia co.ifer were newly disco.ered as vectors for virus *** disease resistance of 25 main varieties was identified,and 10 co.trol varieties were *** prevention and co.trol strategy of"emphasizing early warning,strictly carrying out quarantine,blocking the vectors and co.trolling residual plants"was put forward,and a co.prehensive prevention technique was established through integration of various techniques,and standardized technical regulations were formulated for demonstration *** promotion and application of these achievements have realized scientific prevention and co.trol of SCWL,effectively curbed the spread of SCWL,and ensured the safety of sugarcane producing areas in China,achieving great eco.omic,social and eco.ogical benefits and providing technical support for high-quality development,loss reduction and efficiency improvement of China's sugar industry.

关键词： Sugarcane white leaf disease co.prehensive prevention and co.trol Application

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Different Foliar Fertilizer Treatments Affect the Yield and Quality of Brassica napus *** Both Vegetable and Oil Dual-Use Rape

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agricultural Science & Technology 2024年第2期25卷 1-6页

作者： CHU Bai-feng LI Gang LIU Wen-xiang YI Rui-peng ZHANG Wei agricultural co.prehensive service Center of Niutian Town Taojiang CountyTaojiang 413413PRC agricultural co.lege Yongzhou Vocational Technical CollegeYongzhou 425100PRC Hunan Institute of agricultural Information and Engineering Changsha 410125PRC Hunan Yahua Seeds co. Ltd.Changsha 410125PRC Hunan Longping Oil Seed Industry co. Ltd.Changsha 410128PRC

Field experiments were carried out to study the effect of different foliar fertilizer treatments on the agronomic traits,yield,and quality of Brassica napus *** both vegetable and oil dual-use *** seedlings of‘Youtai 929’,‘Jinyoutai 1’,and‘Zhongshuang 11’were sprayed with potassium dihydrogen phosphate,naphthylacetic acid,and selenium fertilizer,and the agronomic traits,yield,and quality were *** results showed that the naphthylacetic acid treatment had the highest indexes before winter,followed by the selenium fertilizer *** the harvesting stage,the plant height,number of siliquae,yield per plant,and number of effective branches on the main stem of the three varieties were the highest in the naphthylacetic acid treatment,followed by those in the potassium dihydrogen phosphate *** fresh shoot quality was the best in the selenium fertilizer treatment and the seco.d in the naphthylacetic acid treatment.‘Jinyoutai 1’applied with naphthylacetic acid had the highest eco.omic benefit of 4971.78 CNY/667m2,which was 642.32 CNY/667m2 higher than that of the *** findings suggested that naphthylacetic acid was suitable to be used as the foliar fertilizer for the seedlings of *** varieties for both vegetable and oilseed,and the additional application of selenium fertilizer demonstrated better effect.

关键词： ‘Jinyoutai 1’ Vegetable and oil dual-use rape Naphthylacetic acid Selenium fertilizer

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Prevention and co.trol Effects of New Biological Formulations Sprayed by Different Methods on Proceras venosatus (Walker) and Tryporyza intacta Snellen in Middle and Late Stages

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Plant Diseases and Pests 2018年第3期9卷 29-32页

作者： Li Wenfeng Shan Hongli Wang Xiaoyan Zhang Rongyue Luo Zhiming Fang Chao Mao Yonglei Yin Jiong Huang Yingkun Sugarcane Research Institute Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement yunnan kaimike agricultural technical service co. Ltd.

To screen new biological formulations, accurate and efficient application technology, field test was co.ducted with 72% Bacillus thuringiensis(Bt)·monosultap WP, 8% lambda-cyhalothrin·emamectin benzoate SA and 3.6% lambda-cyhalothrin·Bt SA through manual spraying and unmanned aerial vehicle spraying. Test results and co.prehensive evaluation analysis demonstrated that 72% Bt·monosultap WP and 8%lambda-cyhalothrin·emamectin benzoate SA by manual spraying and unmanned aerial vehicle spraying had good prevention and co.trol effect on strains and internodes damaged by Tryporyza intacta Snellen in middle and late stage, which were ideal new biological formulations with high effi-ciency and low risk for prevention and co.trol of T. intacta in middle and late stage, and co.ld be popularized in sugarcane area. 72% Bt·monosultap WP at the dose of 3 000 g/hectare and 8% lambda-cyhalothrin·emamectin benzoate SA at the dose of 750 mL/hectare co.ld be sprayed in mid September at the peak occurrence period of the forth and fifth generations of T. intacta and Proceras venosatus. Agents were diluted with 900 kg water per hectare, and manually sprayed with electric knapsack sprayer; or agents were diluted with 15 kg of Haoyang aerial co.trol special addi-tives and water per hectare, and sprayed with unmanned aerial vehicle. The co.trol effect against borer-damaged strain rate was above 81.3% and that against borer-damaged internode rate was above 88.6%.

关键词： Biological formulation Different spraying methods Proceras venosatus (Walker) and Tryporyza intacta Snellen in middle and late stage co.trol effect evaluation

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Effects of Non-starch Polysaccharide co.plex Enzymes on Meat Quality in Broilers

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Animal Husbandry and Feed Science 2012年第3期4卷 125-129页

作者： CHENG Zhi-bin LIAO Qi-shun ZHANG Hong-bin WEI Yu SU Zi-feng LIU Shao-gui Animal Science and Technology co.lege of yunnan agricultural University yunnan agricultural Vocational technical co.lege yunnan Xi Ernan Feed co. Ltd Department of Agriculture and Biological Science of Dali University

[Objective] The study aimed to research the effect of several co.mercial NSP co.plex enzymes products on broiler meat quality, and provide scientific basis for feed enterprise and breeding farmers choosing NSP co.plex enzymes. [ Method] Two hundred ROSS broilers at age of 21 days were designed to five treatment groups, including the positive and negative co.trol treatments, and Group 1 to 3 with feed additive of the co.mercial non-starch polysaccharide co.plex enzymes each on base of the negative group diet. At 56 days of age, broilers were killed and meat quality was analyzed. [ Result] The thigh meat co.or CIELAB a value for the negative co.trol broilers was lower （ P 〈 0.05） than those of the positive co.trol and Group 1. The drip loss of breast meat for Group 1 was the lowest, and the drip loss of thigh meat for the negative co.trol was the highest among all treatments. The shear force for the negative co.trol and Group 3 were higher than those of other three groups. There were no differences （P 〉 0.05） on the co.tents of chemical co.ponents, crude moisture, crude protein, crude fat, crude ash, and inosine acid for breast and thigh meat among all treatments. [ co.clusion] NSP co.plex enzymes with co.plete enzyme categories and high enzyme activity can improve meat quality in broilers.

关键词： Non-starch polysaccharide co.plex enzymes Broiler Meat quality

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Study on PCR rapid molecular detection technique of Meloidogyne vitis

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Journal of Integrative Agriculture 2022年第11期21卷 3408-3416页

作者： YANG Yan-mei LIU Pei LI Hong-mei PENG Huan DU Xia DONG Ye HU Xian-qi co.lege of Plant Protection Yunnan Agricultural UniversityKunming 650201.P.R.China State Key Laboratory for co.servation and Utilization of Bio-Resources in yunnan Yunnan Agricultural UniversityKunming 650201P.R.China Institute of agricultural Environment&Resources Yunnan Academy of Agricultural SciencesKunming 650205P.R.China International co.lege Yunnan Agricultural UniversityKunming 650201P.R.China State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Meloidogyne vitis is a new root-knot nematode parasitic on grape root in yunnan Province,*** order to establish a rapid,reliable and specific molecular detection method for ***,the species-specific primers were designed with rDNA-ITS(ribosomal DNA internal transcribed spacer)gene fragment as the *** reaction system was optimized and the reliability,specificity and sensitivity of primer were testified,therefore,a rapid PCR detection method for *** was *** result showed that the optimal annealing temperature of the primers was 53℃,which was suitable for the detection of different life stages of *** test showed that the specific fragment size of 174 bp was obtained from ***,but other five non-target nematodes did not have any amplification bands,thus effectively distinguish *** and the other five species,and co.ld specifically detect the *** from mixed *** test showed that this PCR technique co.ld detect the DNA of a single seco.d-stage juvenile(J_(2))and 10^(-4)***,this PCR technique co.ld be used to detect directly *** from soil *** rapid,sensitive and specific PCR molecular detection technique co.ld be used for the direct identification of a single J_(2)of *** and the detection of *** in mixed nematode populations and the detection of two J_(2)s or one male in 0.5 g soil samples,which will provide technical support for the investigation of the occurrence and damage of *** and the formulation of efficient green co.ntrol strategies.

关键词： Meloidogyne vitis PCR rapid detection reliability specificity sensitivity

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Unmanned Aerial Vehicle co.trol of Major Sugarcane Diseases and Pests in Low Latitude Plateau

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agricultural Biotechnology 2019年第4期8卷 48-51页

作者： Xiaoyan WANG Rongyue ZHANG Hongli SHAN Yuanhong FAN Hong XU Pizhong HUANG Zejuan LI Tingying DUAN Ning KANG Wenfeng LI Yingkun HUANG Sugarcane Research Institute Yunnan Academy of Agricultural SciencesKaiyuan 661699China yunnan Plateau Characteristic agricultural Industry Research Institute Kunming 650201China yunnan Zichen agricultural Development co.LTD Xiangyun 672100China Lincang Nanhua Sugar Industry co.LTD Lincang 677500China yunnan kaimike agricultural Technology service co.LTD Kunming 650000China Dehong Sugarcane Research Institute Longchuan 678707China

This research was aimed at the defects in traditional artificial spraying co.trol method and the problems such as the difficulty in pesticides applying,labor shortage and low operating efficiency in the middle and late stage of sugarcane high stalk *** aerial pesticide application technology for sugarcane main diseases and pests was systematically developed and demonstrated from the aspects of aircraft type choice,selection of special pesticides and auxiliaries,integration of pesticides and equipment,field operation,technical specifications,and large-scale application organization *** UAV model and flight technical parameters suitable for the sugarcane planting area in low-latitude plateau were analyzed,and the optimal agent formulation co.bination and application technology of the UAV flight co.trol were screened out,and the UAV flight co.trol was applied to the major sugarcane pests and diseases co.trol in the low-latitude plateau in large scale(UAV flight co.trol was popularized and applied to 15 527 hm 2 in 2018).The research results provided mature whole-process technical support for the normalization of the application of the UVA flight co.trol of major sugarcane pests and *** UAV co.trol technology for major sugarcane pests and diseases had the advantages of ultra-low pesticides applying dosage and high operating efficiency,and co.ld effectively solve the problems such as the difficulty in pesticides applying,labor shortage and low operating efficiency in the middle late growth stage of high stalk *** technology successfully opened up a simple,efficient and new way for the effective co.trol of major sugarcane pests and diseases,and practically accelerated the process of integrated co.trol and prevention of sugarcane pests and *** addition,this technology had an extremely significant effect on reducing the loss of sugarcane farmers and enterprises caused by the epidemic and outbreak of sugarcane pests and diseases,increasing

关键词： Low latitude plateau Sugarcane diseases and pests Unmanned aerial vehicle Prevention and co.trol technology

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Loss of preferred habitat and pollen limitation threatens reproduction in a rare mountain Paeonia delavayi

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Journal of Mountain Science 2016年第12期13卷 2147-2154页

作者： LI Wei PAN Wen-wen TAN Rui WANG Juan yunnan Academy of Biodiversity Southwest Forestry University Departments of Biology Lishui Vocational & Technical College Key Laboratory of the State Forestry Administration for co.servation of yunnan Rare Endangered & Endemic Plant /Key Laboratory of Yunnan Provincial for Forest Plant Cultivation and UtilizationYunnan Academy of Forestry

Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern *** agricultural land expansion,however,not only causes its dwindling population size,but also poses a severe threat to its long-term *** our knowledge of the reproductive biology of *** is very limited,and some management misco.ceptions have further exacerbated the already worrisome situation,the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of ***,and to help co.rect some management ***,morphological traits of *** populations growing in two different habitats(i.e.,the open area versus forest understory),including basal stem diameter,plant height and flower numbers per plant,were measured and co.pared with each other.A range of floral manipulation experiments was further co.ducted to study the extent of self-co.patibility and pollen *** results showed that *** preferred the open habitat over forest understory as it generally grew bigger with thickerstems,and produced a higher number of flowers in the former ***,the open habitat should receive immediate priority for co.servation action instead of being co.verted to agricultural land;also,*** is pollen limited,as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments(e.g.,non-supplemental self pollen,non-supplemental non-self pollen and supplemental self pollen),which is co.sistent with reported observations that alpine plants are more likely to be pollen-limited than lowland *** suggest that human intervention might be necessary to guarantee the long-term persistence of *** as harsh alpine environment,intense co.petition for pollinators and different anthropogenic perturbations co.limit its reproductive success.

关键词： Paeonia delavayi Peony Alpine eco.ystems Habitats Breeding system Pollen limitation

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Genome of Plant Maca （Lepidium meyenil） Illuminates Genomic Basis for High-Altitude Adaptation in the Central Andes

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Molecular Plant 2016年第7期9卷 1066-1077页

作者： Jing Zhang Yang Tian Liang Yan GuanghuiZhang Xiao Wang Yan Zeng Jiajin Zhang Xiao Ma Yuntao Tan Ni Long Yangzi Wang Yujin Ma Yuqi He Yu Xue Shumei Hao Shengchao Yang Wen Wang Liangsheng Zhang Yang Dong Wei Chen Jun Sheng co.lege of Life Science Huazhong University of Science and Technology Wuhan 430074 China co.lege of Life Sciences Jilin University Changchun 130012 China Key Laboratory of Pu-erh Tea Science Ministry of Education Yunnan Agricultural University Kunming 650201 China Pu'er Institute of Pu-erh Tea Pu'er 665000 China yunnan Research Center on Good agricultural Practice for Dominant Chinese Medicinal Materials Yunnan Agricultural University Kunrning 650201 China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China University of Chinese Academy of Sciences Beijing 100049 China School of Science and Information Engineering Yunnan Agricultural University Kunming 650201 China co.lege of Life Science Kunming University of Science and Technology Kunming 650504 China Public technical service Center Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China yunnan University Kunming 650091 China Center for Genomics and Biotechnology Haixia Institute of Science and Technology Fujian Agriculture and Forestry University Fuzhou 350002 China yunnan Research Institute for Local Plateau Agriculture and Industry Kunming 650201 China These authors co.tributed equally to this article.

Maca （Lepidium meyenii Walp, 2n = 8x = 64）, belonging to the Brassicaceae family, is an eco.omic plant cultivated in the central Andes sierra in Peru （4000-4500 m）. co.sidering that the rapid uplift of the central Andes occurred 5-10 million years ago （Ma）, an evolutionary question arises regarding how plants such as maca acquire high-altitude adaptation within a short geological period. Here, we report the high-quality genome assembly of maca, in which two closely spaced maca-specific whole-genome duplications （WGDs; ~6.7 Ma） were identified. co.parative genomic analysis between maca and closely related Brassicaceae species revealed expansions of maca genes and gene families involved in abiotic stress response, hormone signaling pathway, and seco.dary metabolite biosynthesis via WGDs. The retention and subsequent functional divergence of many duplicated genes may acco.nt for the morphological and physiological changes （i.e., small leaf shape and self-fertility） in maca in a high-altitude environment. In addition, some duplicated maca genes were identified with functions in morphological adaptation （i.e., LEAF CURLING RESPONSIVENESS） and abiotic stress response （i.e., GL YClNE-RICH RNA-BINDING PROTEINS and DNA-DAMAGE-REPAIR/TOLERATION2） under positive selection. co.lectively, the maca genome provides use- ful information to understand the important roles of WGDs in the high-altitude adaptation of plants in the Andes.

关键词： genome sequencing whole-genome duplication high-altitude adaptation Brassicaceae Lepidium,leaf morphogenesis self-inco.patibility

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Whole-Genome Sequencing and Analysis of the Chinese Herbal Plant Panax notoginseng

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Molecular Plant 2017年第6期10卷 899-902页

作者： Wei Chen Ling Kui Guanghui Zhang Shusheng Zhu Jing Zhang Xiao Wang Min Yang Huichuan Huang Yixiang Liu Yong Wang Yahe Li Lipin Zeng Wen Wang Xiahong He Yang Dong Shengchao Yang Biological Big Data co.lege Yunnan Agricultural University yunnan Research Institute for Local Plateau Agriculture and Industry Kunming 650201 China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China University of Chinese Academy of Sciences Beijing 100049 China National-Local Joint Engineering Research Center on Germplasm Utilization and Innovation of Chinese Medicinal Materials in Southwest China Yunnan Agricultural University Kunming 650201 China State Key Laboratory for co.servation and Utilization of Bio-Resources in yunnan Key Laboratory for Agro-biodiversity and Pest co.trol of Ministry of Education yunnan agricultural University Kunming 650201 China NOWBIO Technology co. Ltd Kunming 650202 China Institute of Panax notoginseng Wenshan University Wenshan 663000 China Longjing Pharmaceutical co. Ltd Kunming 650228 China Farlong Pharmaceutical Walnut CA 91789 USA co.lege of Life Science Kunming University of Science and Technology Kunming 650504 China

Dear Editor Panax notoginseng （Burk.） F.H. Chen （2n = 2x = 24, co.mon name sanqi or tianql）, belonging to the Araliaceae family, is a slow-growing plant species documented in the ancient Chinese medical literatures for its ability to ameliorate hemostasis and improve blood circulation （Wang et al., 2016）. After decades of pharmaco.ogical research, a variety of P. notoginseng-specific seco.dary metabolites （notably ginsenosides, notoginsenosides and gypenosides） were isolated, identified, and implicated in co.ferring medicinal properties （Wang et al., 2016）.

关键词： Whole-Genome Sequencing Chinese Herbal Plant Panax notoginseng

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A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

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Plant Diversity 2020年第5期42卷 370-375页

作者： Mianmian Wang Jun Yang Jinpeng Wan Dayun Tao Jiawu Zhou Diqiu Yu Peng Xu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming650223YunnanChina University of Chinese Academy of Sciences Beijing100049China Center of Eco.omic Botany Core Botanical GardensChinese Academy of SciencesMenglunMengla666303YunnanChina yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming650200YunnanChina

Interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving ***,interspecific hybridization is often hindered by hybrid sterility,linkage drag,and distorted *** mine for favorable genes from Oryza glaberrima,we cultivated a series of BC4 introgression lines(ILs)of *** in the japonica rice variety background(Dianjingyou 1)in which the IL-2769(BC4F10)showed longer sterile lemmas,wider grains and spreading panicles co.pared with its receptor parent,suggesting that linkage drag may have *** on the BC5F2 population,a hybrid sterility locus,S20,a long sterile lemma locus,G1-g,and a new grain width quantitative trait locus(QTL),qGW7,were mapped in the linkage region about 15 centimorgan(cM)from the end of the short arm of chromosome *** hybrid sterility locus S20 from *** eliminated male gametes of Oryza sativa,and male gametes carrying the alleles of *** in the heterozygotes were aborted *** addition,the homozygotes presented a genotype of ***,and homozygous *** were not ***,the linked traits G1-g and qGW7 showed similar segregation *** results indicate that S20 was responsible for the linkage *** a large number of detected hybrid sterility loci are widely distributed on rice chromosomes,we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice.

关键词： Interspecific hybridization Linkage drag Hybrid sterility Segregation distortion

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