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Molecular cloning,characterization and promoter analysis of LbgCWIN1 and its expression profiles in response to exogenous sucrose during in vitro bulblet initiation in lily

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Horticultural Plant Journal 2024年第2期10卷 545-555页

作者： Cong Gao Shiqi Li Yunchen Xu Yue Liu Yiping Xia Ziming Ren Yun Wu Genomics and Genetic Engineering Laboratory of Ornamental Plants Institute of Landscape ArchitectureCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Laboratory of Flower Bulbs Department of Landscape ArchitectureSchool of Civil Engineering and ArchitectureZhejiang Sci-Tech UniversityHangzhouZhejiang 310018China

Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellular space, are key enzymes participating in sucrose allocation in higher plants. Previous studies have shown that CWINs play an essential role in bulblet initiation process in bulbous crops, but the underlying molecular mechanism remains unclear. Here, a CWIN gene of Lilium brownii var. giganteum(Lbg) was identified and amplified from genomic DNA. Quantitative RT-PCR assays revealed that the expression level of LbgCWIN1 was highly upregulated exactly when the endogenous starch degraded in non-sucrose medium during in vitro bulblet initiation in Lbg. Phylogenetic relationship, motif, and domain analysis of LbgCWIN1 protein and CWINs in other plant species showed that all sequences of these CWIN proteins were highly conserved. The promoter sequence of LbgCWIN1 possessed a number of alpha-amylase-, phytohormone-, light-and stress-responsive cis-elements. Meanwhile, β-glucuronidase(GUS) assay showed that the 459 bp upstream fragment from the translational start site displayed maximal promoter activity. These results revealed that LbgCWIN1 might function in the process of in vitro bulblet initiation and be in the response to degradation of endogenous starch.

关键词： Lilium brownii var.giganteum LbgCWIN1 Phylogenetic analysis Promoter analysis Bulblet initiation

A personalized electronic textile for ultrasensitive pressure sensing enabled by biocompatible MXene/ PEDOT:PSS composite

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Carbon Energy 2024年第3期6卷 224-238页

作者： Yahua Li Wentao Cao Zhi Liu Yue Zhang Ziyan Chen Xianhong Zheng Department of Interventional Radiology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenanChina School of Textile and Garment Anhui Polytechnic UniversityWuhuAnhuiChina Department of Prosthodontics Shanghai Stomatological Hospital and School of StomatologyFudan UniversityShanghaiChina college of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghaiChina

Flexible,breathable,and highly sensitive pressure sensors have increasingly become a focal point of interest due to their pivotal role in healthcare monitoring,advanced electronic skin applications,and disease ***,traditional methods,involving elastomer film-based substrates or encapsulation techniques,often fall short due to mechanical mismatches,discomfort,lack of breathability,and limitations in sensing ***,there is a pressing need,yet it remains a significant challenge to create pressure sensors that are not only highly breathable,flexible,and comfortable but also sensitive,durable,and ***,we present a biocompatible and breathable fabric-based pressure sensor,using nonwoven fabrics as both the sensing electrode(coated with MXene/poly(3,4-ethylenedioxythiophene):polystyrene sulfonate[PEDOT:PSS])and the interdigitated electrode(printed with MXene pattern)via a scalable spray-coating and screen-coating *** resultant device exhibits commendable air permeability,biocompatibility,and pressure sensing performance,including a remarkable sensitivity(754.5 kPa^(−1)),rapid response/recovery time(180/110 ms),and robust cycling ***,the integration of PEDOT:PSS plays a crucial role in protecting the MXene nanosheets from oxidation,significantly enhancing the device's long-term *** outstanding features make this sensor highly suitable for applications in fullrange human activities detection and disease *** study underscores the promising future of flexible pressure sensors in the realm of intelligent wearable electronics,setting a new benchmark for the industry.

关键词： biocompatibility MXene pressure sensor screen printing textile

Oral administration of Bacillus coagulans TQ-35 alleviates allergic responses in OVA-sensitive BALB/c mice

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Food Science and Human Wellness 2024年第3期13卷 1246-1257页

作者： Yifan Wang Shanjun Chen Chong Wang Yi Zhang Hongliang Zeng Linglin Fu Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China college of Food Science Fujian Agriculture and Forestry UniversityFuzhou 350002China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Bacillus coagulans has been extensively studied so far,but there has been a lack of research on its usage in *** this study,we designed to assess the effect of different concentrations of *** on food allergy in a BALB/c mouse model of ovalbumin(OVA)-induced food allergy and its effect on gut *** assessment of symptoms,specific immunoglobulin E(IgE),T-cell differentiation,and related gene expression levels in sensitized mice by assay indicated that high doses of oral *** could alleviate allergic *** with ***,in the high-dose group,significantly reduced IgE and IgG1 levels and modulated the balance of T helper type 1 cell(Th1)and Th2 and the expression of relevant genes in the spleen.16S rRNA analysis showed that probiotics improved the structure of the microbiota,in particular by boosting the percentage of Clostridia,Bacteroides vulgatus and Enterococcus faecium,and by increasing the abundance of microbial species,thereby modulating the immune ***,this study can provide insights into the practical application of *** doses to alleviate OVA allergy.

关键词： Bacillus coagulans TQ-35 Food allergy Ovalbumin(OVA) Gut microbiota

Development and Application of Detection Methods for Capture and Transcription Elongation Rate of Bacterial Nascent RNA

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生物化学与生物物理进展 2024年第9期51卷 2249-2260页

作者： LI Yuan-Yuan WANG Yu-Ting WU Zi-Chun LI Hao-Xuan FEI Ming-Yue SUN Dong-Chang GUALERZI O.Claudio FABBRETTI Attilio GIULIODORI Anna Maria MA Hong-Xia HE Cheng-Guang Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development College of Life SciencesJilin Agricultural UniversityChangchun 130118China School of Biosciences and Veterinary Medicine University of CamerinoCamerino(MC)62032Italy college of biotechnology and Bioengineering Zhejiang University of TechnologyHangzhou 310014China

Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic *** exposure to environmental stimuli,both the internal reference gene and target gene would be *** a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental *** study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the *** The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead *** the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form *** with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription *** Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.

关键词： nascent RNA selection Click Chemistry fluorescence molecular beacon

Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish

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Zoological Research 2024年第4期45卷 791-804页

作者： Wen-Hao Li Zhi-Qiang Li Meng-Di Bu Jia-Zhen Li Liang-Biao Chen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China Laboratory for Marine Biology and biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China International Research Center for Marine Biosciences Ministry of Science and TechnologyCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China

As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish(Danio rerio) ovaries exposed to a temperature gradient ranging from the thermopreferendum temperature of the species,27℃, to lower temperatures of 22℃, 20℃, and 13℃ over a period of two weeks. Comparative metabolomic(six biological replicates for each temperature) and transcriptomic(four biological replicates for each temperature) analyses were conducted under the four temperature conditions. Results indicated that lower temperatures inhibited oocyte development and differential metabolites were involved in steroid hormone production,antioxidant function, and lipid and protein *** reproductive hormones, increased proteolysis,and lipid degradation significantly impeded oocyte development and egg maturation. Notably, a significant increase in bile acid content was noted in the ovaries of the cold-treated fish, indicating that bile acids play a critical role in ovarian failure. Overall, these findings provide valuable insights into the mechanisms governing the reproductive response of fish to cold stress.

关键词： old stress Ovarian failure Metabolome Zebrafish

Natural variation in MORE GRAINS 1 regulates grain number and grain weight in rice

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Journal of Integrative Plant Biology 2024年第7期66卷 1440-1458页

作者： Yingchun Han Qianfeng Hu Nuo Gong Huimin Yan Najeeb Ullah Khan Yanxiu Du Hongzheng Sun Quanzhi Zhao Wanxi Peng Zichao Li Zhanying Zhang Junzhou Li Henan Key Laboratory of Rice Molecular Breeding and High Efﬁciency Production College of AgronomyHenan Agricultural UniversityZhengzhou 450046China Beijing Key Laboratory of Crop Genetic Improvement College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Rice Industrial Technology Research Institute Guizhou UniversityGuiyang 550025China School of Forestry Henan Agricultural UniversityZhengzhou 450046China

Grain yield is determined mainly by grain number and grain *** this study,we identified and characterized MORE GRAINS1(MOG1),a gene associated with grain number and grain weight in rice(Oryza sativa L.),through map-based *** of MOG1 increased grain yield by 18.6%-22.3%under field *** determined that MOG1,a bHLH transcription factor,interacts with OsbHLH107 and directly activates the expression of LONELY GUY(LOG),which encodes a cytokinin-activating enzyme and the cell expansion gene EXPANSIN-LIKE1(EXPLA1),positively regulating grain number per panicle and grain *** variations in the promoter and coding regions of MOG1 between Hap-LNW and Hap-HNW alleles resulted in changes in MOG1 expression level and transcriptional activation,leading to functional *** analysis revealed that Hap-HNW,which results in a greater number and heavier grains,has undergone strong selection but has been poorly utilized in modern lowland rice *** summary,the MOG1-OsbHLH107 complex activates LOG and EXPLA1 expression to promote cell expansion and division of young panicles through the cytokinin pathway,thereby increasing grain number and grain *** findings suggest that Hap-HNW could be used in strategies to breed high-yielding temperate japonica lowland rice.

关键词： grain number grain weight MOG1 natural variation rice

GWASTool:A web pipeline for detecting SNP-phenotype associations

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Fundamental Research 2024年第4期4卷 761-769页

作者： Xin Wang Beibei Xin Maozu Guo Guoxian Yu Jun Wang School of Software Shandong UniversityJinan 250101China Joint SDU-NTU Centre for Artificial Intelligence Research Shandong UniversityJinan 250101China college of Agronomy&biotechnology China Agricultural UniversityBeijing 100193China college of Electrical and Information Engineering Bejing University of Civil Engineering and ArchitectureBeijing 100044China

The genome-wide association study(GWAS)aims to detect associations between individual single nucleotide polymorphisms(SNPs)or SNP interactions and phenotypes to decipher the genetic *** GWAS analysis tools have different focuses and advantages,but suffer a series of tedious and heterogeneous configurations for *** is inconvenient for researchers to simply choose and apply these tools,statistically and biologically analyze their results for different *** address these issues,we develop a user friendly web pipeline GWASTool for detecting associations,which includes simulation data generation,associated loci detection,result visualization,analysis and *** provides a unified and plugin-able framework to encapsulate the heterogeneity of GWAS algorithms,simplifies the analysis steps and energizes GWAS *** is implemented in Java and is freely available for public use at http://***/*** website hosts a comprehensive collection of resources,including a user manual,description of integrated algorithms,data examples and standalone version for download.

关键词： Genome-wideassociation studies SNP interactions Associated loci detection SNP visualization Web server

Endogenous biosynthesis of docosahexaenoic acid(DHA)regulates fish oocyte maturation by promoting pregnenolone production

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Zoological Research 2024年第1期45卷 176-188页

作者： Yi Li Xuehui Li Ding Ye Ru Zhang Chengjie Liu Mudan He Houpeng Wang Wei Hu Yonghua Sun State Key Laboratory of Freshwater Ecology and biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhanHubei 430072China college of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hubei Hongshan Laboratory WuhanHubei 430070China

Omega-3 polyunsaturated fatty acids(n-3 PUFAs),particularly docosahexaenoic acid(22:6n-3,DHA),play crucial roles in the reproductive health of vertebrates,including ***,the underlying mechanism related to this phenomenon remains largely *** this study,we employed two zebrafish genetic models,i.e.,elovl2^(-/-)mutant as an endogenous DHAdeficient model and fat1(omega-3 desaturase encoding gene)transgenic zebrafish as an endogenous DHA-rich model,to investigate the effects of DHA on oocyte maturation and *** show that the elovl2^(-/-)mutants had much lower fecundity and poorer oocyte quality than the wild-type controls,while the fat1 zebrafish had higher fecundity and better oocyte quality than wildtype *** deficiency in elovl2^(-/-)embryos led to defects in egg activation,poor microtubule stability,and reduced pregnenolone *** study revealed that DHA promoted pregnenolone synthesis by enhancing transcription of cyp11a1,which encodes the cholesterol side-chain cleavage enzyme,thereby stabilizing microtubule assembly during *** turn,the hypothalamic-pituitary-gonadal axis was enhanced by *** conclusion,using two unique genetic models,our findings demonstrate that endogenously synthesized DHA promotes oocyte maturation and quality by promoting pregnenolone production via transcriptional regulation of cyp11a1.

关键词： Docosahexaenoic acid Oocyte maturation Oocyte quality Pregnenolone Microtubule

Pharmacological Effects and Mechanism of Esculentoside A

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Medicinal Plant 2024年第4期15卷 26-28页

作者： Anqi WANG Jundong GUAN Changtao CAI Jiazhu LI Jingjing WEN Chenghao JIN college of Life Science and biotechnology Heilongjiang Bayi Agricultural UniversityDaqing 163319China

Extensive studies have found that Esculentoside a(EsA)has a variety of pharmacological effects,such as anti-inflammatory,anti-bacterial,anti-tumor and the treatment of *** the present paper,the pharmacological effects and related mechanisms of EsA in recent years were reviewed,in order to provide a theoretical reference for further research and development of EsA.

关键词： Esculentoside A(EsA) Anti-inflammation Anti-bacterium Anti-tumor Arthritis