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Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica(Laminariales,Phaeophyta)

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Chinese Journal of Oceanology and Limnology 2014年第2期32卷 377-389页

作者：邵展茹刘福利李秋莹姚建亭段德麟 institute of Oceanology Chinese Academy of Sciences University of Chinese Academy of Sciences yellow sea Fisheries research institute Chinese Academy of Fishery Sciences

Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments, and consequently may have highly efficient ribulose-1, 5-bisphosphate carboxylase/ oxygenase （Rubisco） activity for carbon assimilation. In our study, we cloned the full-length Rubisco gene from *** （SJ-rbc）. It contained an open reading frame for a large subunit gene （SJ-rbcL） of 1 467 bp, a small subunit gene （SJ-rbcS） of 420 bp, and a SJ-rbcL/S intergenie spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa, 5.81 and 15,84 kDa, 4.71, respectively. After induction with 1 mmol/L isopropyl-β-D- thiogalactopyranoside for 5 h and purification by Ni2＋ affinity chromatography, electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations： increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes, mRNA levels of SJ-rbcL, carbonic anhydrase, Calvin-Benson- Bassham cycle-related enzyme, and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly, expression of these genes was up-regulated under blue light irradiance at 350 umol/（m2.s）. Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.

关键词： Saccharina japonica Rubisco photosynthetic genes transcription protein expression carboxylase activity

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Westerlies Affecting the seasonal Variation of Water Vapor Transport over the Tibetan Plateau Induced by Tropical Cyclones in the Bay of Bengal

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Advances in Atmospheric Sciences 2024年第5期41卷 881-893页

作者： Xiaoli ZHOU Wen ZHOU Dongxiao WANG Qiang XIE Lei YANG Qihua PENG Key Laboratory of Polar Atmosphere-ocean-ice System for Weather and Climate Ministry of Education&Department of Atmospheric and Oceanic Sciences&Institute of Atmospheric SciencesFudan UniversityShanghai 200438China School of Marine Sciences Sun Yat-Sen UniversityZhuhai 519082China institute of Deep-sea Science and Engineering Chinese Academic SciencesSanya 572000China State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academic ScienceGuangzhou 510301China Scripps Institution of Oceanography University of CaliforniaSan DiegoLa JollaCalifornia 92093USA

This study investigates the activity of tropical cyclones(TCs)in the Bay of Bengal(BOB)from 1979 to 2018 to discover the mechanism affecting the contribution rate to the meridional moisture budget anomaly(MMBA)over the southern boundary of the Tibetan Plateau(SBTP).May and October–December are the bimodal phases of BOB TC frequency,which decreases month by month from October to December and is relatively low in ***,the contribution rate to the MMBA is the highest in *** seasonal variation in the meridional position of the westerlies is the key factor affecting the contribution *** relatively southern(northern)position of the westerlies in November and December(May)results in a lower(higher)contribution rate to the *** mechanism is confirmed by the momentum *** water vapor enters the westerlies near the trough line,the resultant meridional acceleration is directed *** follows that the farther north the trough is,and the farther north the water vapor can be *** water vapor enters the westerlies from the area near the ridge line,for Type-T(Type-R)TCs,water vapor enters the westerlies downstream of the trough(ridge).Consequently,the direction of the resultant meridional acceleration is directed south and the resultant zonal acceleration is directed east(west),which is not conducive to the northward transport of water *** is especially the case if the trough or ridge is relatively south,as the water vapor may not cross the SBTP.

关键词： tropical cyclone Tibetan Plateau Bay of Bengal moisture budget weste

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Genetic identification of two species of Pleuronichthys by DNA barcoding

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Chinese Journal of Oceanology and Limnology 2011年第5期29卷 967-972页

作者：张辉张岩高天翔李鹏飞徐汉祥 Fisheries College Ocean University of China yellow sea Fisheries research institute Chinese Academy of Fishery Sciences Marine Fisheries research institute of Zhejiang Province

DNA barcoding is a new method for biological taxonomy, offering the ability to identify species from fragments in any life-history stage. Pleuronichthys cornutus and P. japonicus are two morphologically similar species. Pleuronichthys japonicus has never been found previously in China. However, in this study, we identified both species using DNA barcoding （cytochrome c oxidase subunit I （COI））, the mtDNA control region and cytochrome b. The results reveal that： l） intraspecific variation in the DNA barcode is much less than interspecific variation; 2） the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3） COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.

关键词： Pleuronichthys DNA barcoding COI CR Cyt b

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Effects of temperature fluctuations on cuttlebone formation of cuttlefish Sepia esculenta

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Chinese Journal of Oceanology and Limnology 2012年第4期30卷 547-553页

作者：雷舒涵张秀梅刘松林陈四清 Key Laboratory of Mariculture Ministry of EducationOcean University of China yellow sea Fisheries research institute Chinese Academy of Fishery Sciences

The morphological characteristics and the cuttlebone formation of Sepia esculenta exposed to different water temperature fluctuations were investigated under laboratory conditions. Temperature fluctuation cycles （15 cycles, 60 d in total） consisted of the following three regimes of 4 d duration： keeping water temperature in 26℃ for 3 d （Group A）, 2 d （Group B）, 0 d （Group C, control）; then keeping water temperature in 16℃ for the next 1, 2, 4 d. No significant difference in the survival rate was observed between the control and temperature fluctuation groups （P〉0.05）. Lamellar depositions in a temperature fluctuation cycle were 2.45±0.02 for Group A, 2.00±0.02 for Group B, and 1.78±0.02 for Group C （P〈0.05）. The relationship between age and number of lamellas in the cuttlebone of S. esculenta under each water temperature fluctuation could be described as the linear model and the number of lamellas in the cuttlebone did not correspond to actual age. Group A had the highest cuttlebone growth index （CGI）, the lowest locular index （LI）, and inter-streak distances comparing with those of control group. However, the number of lamellas and LI or CGI showed a quadratic relationship for each temperature fluctuation group. In addition, temperature fluctuations caused the breakage of cuttlebone dark rings, which was considered a thermal mark. The position of the breakage in the dark rings was random. This thermal mark can be used as supplementary information for marking and releasing techniques.

关键词： Sepia esculenta cuttlebone formation temperature fluctuations cuttlebone growth index locular index inter-streak distances

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Identification of SNP markers correlated with the tolerance of low-salinity challenge in swimming crab(Portunus trituberculatus)

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Acta Oceanologica Sinica 2019年第8期38卷 41-47页

作者： Yanyan Feng Dening Zhang Jianjian Lv Baoquan Gao Jian Li Ping Liu Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences

Water salinity condition is an important factor for artificial propagation of the swimming crab(Portunus trituberculatus). Low salinity(LS)-resistant strains are preferred by crab industries. Single nucleotide polymorphism(SNP), the third generation of molecular markers, can be utilized in the breeding of LS-resistant species of P. trituberculatus. Our earlier study identified 615 genes differentially expressed in low-salinity stress compared to the controls. Although thousands of SNP loci have been found, it is hard to identify a SNP marker in correlation with a desired trait. In this study, time-of-flight mass spectrometry(TOF-MS), as an efficient method to select SNPs for the tolerance of LS challenge, was utilized for SNP typing. Fifty gene segments were amplified based on comparative transcriptomics in our earlier study, a total of 18 511 bp DNA fragments were amplified,and eighty-five SNP markers were found. The frequency of the SNPs was estimated to be 0.46 per 100 base pairs of DNA sequences. The rate of the conversion mutation was 81%, while the transversion mutation was 19%. The mutation rate of the G/T(C/A), A/T and G/C was 26%, 12% and 7%, respectively. Eight SNP markers were found to significantly correlate with the adaption of low salinity. Of the eight SNP markers, three linked-SNPs were found in the cuticle proportion gene, and another three SNPs were found in three new genes, and the rest two were found in aquaporin gene and chloride channel gene. The development of these SNP markers found in our study could be primarily used for breeding LS-resistant strains of P. trituberculatus.

关键词： Portunus trituberculatus low salinity time-of-flight mass spectrometry single nucleotide polymorphism SNP

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Burial fluxes and sources of organic carbon in sediments of the central yellow sea mud area over the past 200 years

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Acta Oceanologica Sinica 2015年第10期34卷 13-22页

作者： YANG Shu YANG Qian LIU Sai CAI Deling QU Keming SUN Yao Key Laboratory for Sustainable Utilization of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

Long-term changes of composition, sources and burial fluxes of TOC （total organic carbon） in sediments of the central yellow sea mud area and their possible affecting factors are discussed in this paper. Firstly, similarity analysis is employed to confirm that the carbon burial features resulted from two collected cores are typical in the central yellow sea mud area where YSWC （yellow sea Warm Current） is prevalent. On this basis, the burial flux of TOC here was considered to be 235.5-488.4 pmol/（cm^2.a） since the first industrial revolution, accounting for about 70%-90% among burial fluxes of TC （total carbon） in the sediments. Compared TOC/TC ratio in the two cores with that in other marine sediments worldwide, we suggest that the growth of calcareous/non-calcareous organisms and dissolution of IC （inorganic carbon） are important factors controlling the TOC/TC ratio in sediment. Results of two-end mixed model based on fi13C data indicate that marine-derived organic carbon （OCa） is the main part among total burial organic carbon which accounts for a ratio over 85%. Due to the high TOC/TC ratio in the two cores, TC in the sediments also mainly exists as OCa, and the proportion of OCa is about 60%-80%. Away from the shore and relatively high primary production in upper waters are the main reasons that OCa is predominant among all burial OC in sediments of the central yellow sea mud area. Burial of OC in this mud area is probably mainly influenced by the human activities. Although the economic development during the late 19th century caused by the first industrial revolution in China did not obviously increase the TOC burial fluxes in the sediments, the rise of industry and agriculture after the founding of new China has clearly increased the TOC burial flux since 1950s. Otherwise, we also realize that among TC burial fluxes, TIC account for about 10%-30% in sediments of the central yellow sea mud area, so its burial could not be simply ignored here. Distinct from TOC

关键词： the central yellow sea mud area organic carbon burial flux source long-term changes human activities

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Evolution of Cenozoic sedimentary architecture in Central and Southern South China sea basins

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Journal of Palaeogeography 2024年第1期13卷 35-53页

作者： Wu Tang Xiao-Jun Xie Yi-Bo Wang Lian-Qiao Xiong Jia Guo Xin Li China National Offshore Oil Corporation research institute Beijing 100028China

The Central and Southern South China sea(CSSCS) has a complex tectonic dynamic background and abundant oil and gas resources, which has always been a hot topic of academic and industrial ***, systematic analyses are still lacking regarding its sediment filling structure and evolution, mostly due to limited borehole penetration and poor quality of seismic reflection data for deeply buried sequences. No consensus has been reached yet on the sedimentary infilling processes, which impeded the reconstruction of the palaeogeography of Southeast Asia and the oil-and-gas exploration undertakings. Here, we illustrate the Cenozoic sedimentary evolution of the CSSCS region by synthesizing relevant data from previous literature and our own observations and displaying the evolution of depositional systems in sequential reconstructions. Besides, the controlling factors of preferred sedimentary scenarios in the CSSCS incorporate the latest interpretations of the spreading of South China sea(SCS) as well as the demise of the hypothetical Proto-South China sea(PSCS). The results show that there are three types of sedimentary basins in the CSSCS(foreland,strike-slip, and rift basins) with different sedimentary filling structures. The foreland basins formed a depositional pattern of ‘transition from deep water to shallow water environments', dominated by deep-water depositional systems which were formed before the Early Oligocene with submarine fans developed. Later,the foreland basins were gradually dominated by shallow-water depositional systems with deltas and shallow marine facies. The strike-slip basins showed the depositional architecture of ‘transition from lake to marine environments', i.e. the basins were dominated by lacustrine deposits during the Eocene and evolved into the marine depositional environment since Oligocene with delta developed in the western part of the basin. The depositional evolution of rift basins illustrated the characteristics of 'transiti

关键词： Central and Southern South China sea Sedimentary filling Main controlling factors Proto-South China sea South China sea

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Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

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Journal of Oceanology and Limnology 2024年第3期42卷 893-904页

作者： Yanan LI Zongfu CHEN Haibin ZHANG Ruoyu LIU Shuichun CHEN Li LIN Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding College of Animal Sciences and TechnologyZhongkai University of Agriculture and EngineeringGuangzhou 510222China institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanya 572000China College of Marine Science Fujian Agriculture and Forestry UniversityFuzhou 350002China Zhanjiang Tianli sea Cucumber New Technology Development Co. Ltd.Zhanjiang 524000China

Manganese superoxide dismutase(MnSOD)is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in *** a dark,high-pressure,and low-temperature deep-sea environment,MnSOD is essential for the survival of sea *** MnSODs were identified from the transcriptomes of deep and shallow-sea sea *** explore their environmental adaptation mechanism,we conducted environmental selection pressure analysis through the branching site model of PAML *** obtained night positive selection sites,and two of them were significant(97F→H,134K→V):97F→H located in a highly conservative characteristic sequence,and its polarity c hange might have a great impact on the function of MnSOD;134K→V had a change in piezophilic a bility,which might help MnSOD adapt to the environment of high hydrostatic pressure in the *** further study the effect of these two positive selection sites on MnSOD,we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and *** show that 97F→H,134K→V might improve MnSOD’s efficiency of scavenging superoxide a nion and its ability to resist high hydrostatic pressure by moderately reducing its *** above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity,piezophilic behavior,and local *** study revealed the correlation between MnSOD and extreme environment,and will help improve our understanding of the organism’s adaptation mechanisms in deep sea.

关键词： Holothuroidea environmental adaptation positive selection point mutation

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Small molecule deoxynyboquinone triggers alkylation and ubiquitination of Keap1 at Cys489 on Kelch domain for Nrf2 activation and inflammatory therapy

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Journal of Pharmaceutical Analysis 2024年第3期14卷 401-415页

作者： Ke-Gang Linghu Tian Zhang Guang-Tao Zhang Peng Lv Wen-Jun Zhang Guan-Ding Zhao Shi-Hang Xiong Qiu-Shuo Ma Ming-Ming Zhao Meiwan Chen Yuan-Jia Hu Chang-Sheng Zhang Hua Yu State Key Laboratory of Quality research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SARChina Macao Centre for research and Development in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SARChina Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Key Laboratory of Marine Materia MedicaRNAM Center for Marine MicrobiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou510301China State Key Laboratory of Functions and Applications of Medicinal Plants&School of Pharmaceutical Sciences Guizhou Medical UniversityGuiyang550025China

Activation of nuclear factor erythroid 2-related factor 2(Nrf2)by Kelch-like ECH-associated protein 1(Keap1)alkylation plays a central role in anti-inflammatory ***,activators of Nrf2 through alkylation of Keap1-Kelch domain have not been ***(DNQ)is a natural small molecule discovered from marine *** current study was designed to investigate the anti-inflammatory effects and molecular mechanisms of DNQ via alkylation of *** exhibited significant anti-inflammatory properties both in vitro and in *** pharmacophore responsible for the anti-inflammatory properties of DNQ was determined to be theα,β-unsaturated amides moieties by a chemical reaction between DNQ and *** exerted anti-inflammatory effects through activation of Nrf2/ARE ***1 was demonstrated to be the direct target of DNQ and bound with DNQ through conjugate addition reaction involving *** specific alkylation site of DNQ on Keap1 for Nrf2 activation was elucidated with a synthesized probe in conjunction with liquid chromatography-tandem mass *** triggered the ubiquitination and subsequent degradation of Keap1 by alkylation of the cysteine residue 489(Cys489)on Keap1-Kelch domain,ultimately enabling the activation of *** findings revealed that DNQ exhibited potent anti-inflammatory capacity throughα,β-unsaturated amides moieties active group which specifically activated Nrf2 signal pathway via alkylation/ubiquitination of Keap1-Kelch domain,suggesting the potential values of targeting Cys489 on Keap1-Kelch domain by DNQ-like small molecules in inflammatory therapies.

关键词： Deoxynyboquinone Anti-inflammation Target Keap1/Nrf2 Alkylation Ubiquitination

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Imaging the western boundary of the Sichuan Craton from multiple geophysical observations

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Journal of Earth Science 2024年

作者： Qihang Yin Xi Xu Xiaodong Yang Xuhua Shi Lei Wu Andrew V.Zuza Liu Yang Hefeng Tian Yuanhai Shu Shufeng Yang Nevada Bureau of Mines and Geology， University of Nevada China Aero Geophysical Survey and Remote Sensing Center for Natural Resources，China Geological Survey School of Earth Sciences， Zhejiang University research Center for Structures in Oil and Gas Bearing Basins， Ministry of Education Key Laboratory of Ocean and Marginal sea Geology， South China sea institute of Oceanology， Innovation Academy of South China sea Ecology and Environmental Engineering， Chinese Academy of Sciences School of Mechanical and Electrical Engineering， Hubei Open University

The boundary between the cratonic and orogenic lithospheres is one of the most significant seismogenic zones characterized by strong lithospheric deformation. As the essential tectonic block bounding the eastern Tibetan Plateau， the Sichuan Craton (SCC) resists the eastward escape of Plateau’s crustal materials， resulting in the uplift of the Songpan-Ganzi Block (SGB) and the crustal deformation of the Longmenshan tectonic belt (LMTB). To uncover the compressional structures and deformational mode of the LMTB and SCC， it is crucial to determine the precise location and geometry of the western boundary of the SCC. To address this issue， the lithospheric properties of the obduction SGB， the underthrusting SCC， and the LMTB were analyzed using a series of geophysical data， including seismic reflection profiles， magnetotellurics， aeromagnetics， gravity， and seismic tomography. The crust of the SGB is characterized by low magnetism， low seismic velocity， low resistivity and low Bouguer gravity， while that of the SCC features non-uniform high magnetism， high seismic velocity， high resistivity and high Bouguer gravity. The LMTB， as the boundary between the SGB and SCC， shows the geophysical characteristics similar to the SCC along the southern and central segments. The integration of all these geophysical observations suggests that the SCC’s western boundary follows the Yingxiu-Beichuan fault along the northern segment， without obvious underthrust. However， this boundary is located west of the Wenchuan-Maoxian fault zone along the southern and central segments， with distinct westward wedging/underthrusting. The irregular geometry of the SCC’s western boundary can further explain the variation in structural deformation along the LMTB. By comparing the differences in crustal thickness and lithospheric strength between the SGB and SCC， this study suggests that the varying crustal strength between tectonic blocks may be the controlling factor for the irregular geometry of the

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