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Acta Oceanologica Sinica 2011年第3期30卷 92-99页

作者： LI Jialin BAI Jie GAO Huiwang LIU Guangxing Key Laboratory of Marine Environmental science and Ecology of Ministry of Education College of Environmental Science and Engineering Ocean University of China Qingdao 266100 China yantai Institute of Coastal Zone Research Chinese Academy of Sciences Yantai 264003 China

The spatial distribution of ammonia-oxidizing Betaproteobacteria （βAOB） was investigated by FISH （fluorescence in situ hybridization） and DGGE （denaturing gradient get electrophoresis） techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of βAOB ranged from 1.87× 10^5 to 3.53×10^5 cells/g of sediment. βAOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the βAOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition ofβAOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that theβAOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between βAOB abundance and nitrification estimates, the abundance and diversity of βAOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.

关键词： ammonia-oxidizing Betaproteobacteria (βAOB) diversity abundance nitrification surface sediment Changiiang River Estuarv (CRE)

Reproductive biology of Hemitripterus villosus in the Bohai Strait,china

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Journal of Oceanology and Limnology 2024年第3期42卷 881-892页

作者： Ping SUN Song QIN Zhengyi LIU Longchuan ZHUANG Key Laboratory of Coastal Biology and Biological Resource Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese academy of sciences Beijing 100049China Center for Ocean Mega-science Chinese Academy of SciencesQingdao 266071China

To understand the reproductive biology of Hemitripterus villosus in the Bohai Strait,and fill the key knowledge gap in verifying reproductive seasonality and documenting gonadotropin dependent reproductive cycle of this species,a year-round detail study was conducted for the first *** total,263 individuals were randomly sampled off the coasts of the Bohai *** show that and the overall female-to-male ratio(1.35:1)did not differ significantly from the 1:1 sex *** length and weight relationship for females and males were regressed,depicting an isometric growth for both *** reproductive phases of the complete gonadotropin dependent reproductive cycle in both sexes were *** monthly variations in the gonadosomatic and hepatosomatic indexes for both sexes further indicated that this species spawns a single batch of eggs per year and the peak spawning period is from October to *** average diameter of eggs ranged from 2.10 to 3.57 *** length at first sexual maturity was 224.87 mm for females and 241.26 mm for *** positive relationships between absolute fecundity and fish size,and also gonadal weight were *** from the Bohai Strait exhibited smaller body size,smaller egg diameter,lower gonadosomatic index,lower absolute fecundity,and higher population extinction risk than the other populations’studied *** findings contribute to understanding the status of this isolated westernmost population,and we emphasize that more efficient protection measures for the species and control measures are urgently required to improve the coastal environment in the Bohai Strait.

关键词： Hemitripterus villosus Bohai Strait fecundity length at first maturity reproductive seasonality

FOLMS-AMDCNet:an automatic recognition scheme for multiple-antenna OFDM systems

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Journal of Systems Engineering and Electronics 2023年第2期34卷 307-323页

作者： ZHANG Yuyuan YAN Wenjun ZHANG Limin LING Qing academy of Aeronautical Operations Service Naval Aviation UniversityYantai 264001China

The existing recognition algorithms of space-time block code(STBC)for multi-antenna(MA)orthogonal frequencydivision multiplexing(OFDM)systems use feature extraction and hypothesis testing to identify the signal types in a complex communication ***,owing to the restrictions on the prior information and channel conditions,these existing algorithms cannot perform well under strong interference and noncooperative communication *** overcome these defects,this study introduces deep learning into the STBCOFDM signal recognition field and proposes a recognition method based on the fourth-order lag moment spectrum(FOLMS)and attention-guided multi-scale dilated convolution network(AMDCNet).The fourth-order lag moment vectors of the received signals are calculated,and vectors are stitched to form two-dimensional FOLMS,which is used as the input of the deep learning-based ***,the multi-scale dilated convolution is used to extract the details of images at different scales,and a convolutional block attention module(CBAM)is introduced to construct the attention-guided multi-scale dilated convolution module(AMDCM)to make the network be more focused on the target area and obtian the multi-scale guided ***,the concatenate fusion,residual block and fully-connected layers are applied to acquire the STBC-OFDM signal *** experiments show that the average recognition probability of the proposed method at−12 dB is higher than 98%.Compared with the existing algorithms,the recognition performance of the proposed method is significantly improved and has good adaptability to environments with strong *** addition,the proposed deep learning-based model can directly identify the pre-processed FOLMS samples without a priori information on channel and noise,which is more suitable for non-cooperative communication systems than the existing algorithms.

关键词： blind signal identification(BSI) space-time block code(STBC) orthogonal frequency-division multiplexing(OFDM) deep learning fourth-order lag moment spectrum(FOLMS)

Sediment bacterial communities are more complex in coastal shallow straits than in oceanic deep straits

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Journal of Oceanology and Limnology 2018年第5期36卷 1643-1654页

作者： LIU Hongmei WANG Bin HU Xiaoke School of Ocean Yantai UniversityYantai 264003China Key Laboratory of Coastal Biology and Bioresource Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China

Straits are ideal models to investigate the bacterial community assembly in complex hydrological environments. However, few studies have focused on bacterial communities in them. Here, comparable bacterial communities in costal shallow Bohai Strait(BS) and oceanic deep Fram Strait(FS) were studied. The Shannon and Chao1 indices were both higher in BS than in FS. The relative abundances of the classes Deltaproteobacteria and Bacilli and the family Halieaceae were higher in BS than in FS, in contrast to the families OM1_clade and JTB255_marine_benthic_group, revealing typical characteristics of bacterial communities in coastal and oceanic regions. Cluster analysis based on the Bray-Curtis index showed that samples were clustered by depth layer in FS and BS, indicating that structures of bacterial communities would diff er with increasing water depth in straits. Additionally, the cluster relationships among samples in abundant and rare communities were both similar to those in entire communities. However, the dissimilarities among samples showed a descending order as rare communities, entire communities and abundant communities. Network analysis indicated that the BS network was obviously more complex than the FS network. Filamentous bacteria Desulfobulbaceae exhibited high degree values in BS but not in FS, indicating key roles of Desulfobulbaceae in the BS. Our study provides different and common evidences for understanding microbial ecology in coastal shallow and oceanic deep straits.

关键词： bacterial communities Bohai Strait Fram Strait currents microbial ecological network

Eff ects of temperature on photosynthetic performance and nitrate reductase activity in vivo assay in Gracilariopsis lemaneiformis (Rhodophyta)

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Journal of Oceanology and Limnology 2021年第1期39卷 362-371页

作者： Zhihai ZHONG Zhengyi LIU Longchuan ZHUANG Wanlin SONG Weizhou CHEN yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China Shantou University Shantou 515063China

Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in china at *** being acclimated to diff erent growth temperatures(15,20,25,and 30°C)for 7 days,the relative growth rate(RGR),nitrate reductase activity,soluble protein content and chlorophyll a fl uorescence of *** were *** show that RGR was markedly aff ected by temperature especially at 20°C at which *** exhibited the highest eff ective quantum yield of PSII[Y(II)]and lightsaturated electron transport rate(ETR max),but the lowest non-photochemical *** of growth temperature,the nitrate reductase activity increased with the incubation temperature from 15 to 30°*** addition,the greatest nitrate reductase activity was found in the thalli grown at 20°*** value of temperature coeffi cient Q10 of alga cultured in 15°C was the greatest among those of other temperatures *** indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity,and the relationship among growth,photosynthesis,and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature.

关键词： chlorophyll a fl uorescence Gracilariopsis lemaneiformis growth nitrate reductase activity

Invasion Possibility and Potential Effects of Rhus typhina on Beijing Municipality

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Journal of Integrative Plant Biology 2008年第5期50卷 522-530页

作者： Guangmei Wang Gaoming Jiang Shunli Yu Yinghao Li Hui Liu State Key Laboratory of Vegetation science and Environmental Changes Institute of Botany the Chinese Academy of Sciences Beijing 100093 China yantai Institute of Coastal Zone Research for Sustainable Development the Chinese Academy of Sciences Yantai 264003 China Graduate School of the Chinese academy of sciences Beijing 100049 China

Rhus typhina, an alien species introduced from North America, was identified as a main afforestation species in Beijing municipality. However, its invasiveness is still at odds. To clarify this problem, we applied the North American Screening System and the Australian Screening System to preliminarily predict its invasion possibility. Both screening systems gave the same recommendation to ＂reject＂. The geographical distribution was surveyed, with the population features of R. typhina against the native plant communities being assessed. With anthropogenic assistance, R. typhina has been scattered on almost all habitats from downtown to mountains, including roadsides, farmlands and protected areas. As a clonal shrub, R. typhina possessed a high spreading rate, varying from 6.3 m/3 years at sterile habitats to 6.7 m/3 years at fertile ones. Significantly lower species richness, individual density and diversity were observed in the R. typhina community than those of the native Vitex negundo ***. heterophylla （Franch.） Rehd. community at both sterile and fertile habitats. Continual wide plantation of R. typhina may further foster its population expansion, which helps the species to overcome spatial isolation, The fact that each root fragment can develop into a new individual makes R. typhina very difficult to be eradicated once established. From a biological point of view, we believe that R. typhina is a plant invader in Beijing. We therefore suggest the government should remove the name of R. typhina from the main tree species list in afforesUng Beijing.

关键词： alien species Beijing ecological impact evaluation invasion Rhus typhina

Effects of acute and chronic thermal stress on survival,apoptosis,and transcriptional responses of Scapharca broughtonii

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Journal of Oceanology and Limnology 2023年第6期41卷 2363-2373页

作者： Desheng ZOU Weian CAO Guilong LIU Junhao NING Xia LU Jinjing WANG Min CHEN Bo LIU Jinsheng ZHANG Chunde WANG yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China University of Chinese academy of sciences Beijing 100049China yantai Spring-Sea AquaSeed Co. Ltd.Yantai 264006China Qingdao Agricultural University Qingdao 266109China Zhaoyuan Xiadian Agricultural Technology Promotion Station Yantai 265415China

Ocean warming is altering the habitats of marine invertebrates,which has resulted in an increased physiological stress to marine molluscs,especially those intertidal bivalves,such as the ark shell Scapharca *** investigated the physiological and transcriptional responses of ark shells to acute and chronic thermal stress results showed that at 33℃,a significantly higher cumulative mortality(55.7%)occurred under acute thermal stress than chronic thermal *** apoptosis rate of hemocytes was sustained at higher levels and the necrosis rate was increased significantly in a time-dependent manner under acute thermal ***,under chronic thermal stress,the apoptosis and necrosis rates exhibited similar change trends:a rapid increase followed by a gradual decline and sustained at a relatively high level until the end of the *** expressions of heat shock protein genes(HSP20 and HSP90),apoptosis-related genes(TRAF6,GRP78,NIX,and Casp-3),antioxidative-related genes(GST and MRP)and cellular detoxification-related genes(HbⅡB,NOS-1,HO-1,and ENO-1)were upregulated significantly under both acute and chronic thermal *** results demonstrated that the anti-apoptotic system,antioxidant defense system,cellular detoxification system,and heat shock proteins(HSPs)played vital roles for ark shells in response to thermal *** acute thermal stress can result in irreversible damage to marine molluscs,it is thus advised that chronic thermal stress should be used to select thermal-resistant ark shell strains.

关键词： Scapharca broughtonii thermal stress apoptosis necrosis gene expression

Study of the Laser-MIG Hybrid Welded Al-Mg Alloy

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稀有金属材料与工程 2011年第S4期40卷 115-119页

作者： Tan Bing,Wang Youqi,Cheng Donggao,Liu Hongwei,Ma Zhihua Welding Material and Engineering,Ningbo branch of china academy of ordnance science,Ningbo 315103,china china Acad ordnance Sci Ningbo Branch Ningbo 315103 Zhejiang Peoples R China

The technology of laser-MIG hybrid welding is hotspot in welding researched field at *** can improve the velocity of the welding,reduce the distortion of the welding,optimize the structure of the welding joint and etc..The 5052 aluminum alloy of the 10mm thick was welded by the laser-MIG hybrid *** structure,the alloy elements profile and the mechanical property of the welded joint are researched by the optical microscope,SEM,sclerometer and etc..The results showed:The medium thick Al alloy was welded in high speed by the laser-MIG hybrid *** appearance of the welding joint is *** weld joint and the weld interface are *** intenerate region in the welding joint is *** tensile strength in welding joint has achieved 94.4% of that in base metal.

关键词： aluminum alloy laser-MIG hybrid welding microstructure mechanical property

Three nitrogen-containing metabolites from an algicolous isolate of Trichoderma asperellum

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Marine Life science & Technology 2020年第2期2卷 155-160页

作者： Yinping Song Fengping Miao Xiuli Yin Naiyun Ji yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China

As one of the most productive species,Trichoderma asperellum,especially isolated from marine algae,has contributed a number of new terpenes and nitrogen-bearing compounds,encouraging our continuous efforts to further explore ***,three new nitrogen-containing secondary metabolites,including cyclo(L-5-MeO-Pro-L-5-MeO-Pro)(1),5'-acetoxy-deoxycyclonerin B(2),and 5'-acetoxy-deoxycyclonerin D(3)were isolated from the marine alga-derived endophytic fungus Trichoderma asperellum *** structures and relative configurations were fully elucidated through spectroscopic techniques,mainly including 1D/2D NMR and *** absolute configurations of 1 and 2 were ascertained on the basis of X-ray diffraction(Cu Kα radiation)and ECD data,*** isolation of these new nitrogen-bearing compounds adds to the structural diversity of marine algicolous Trichoderma spp.,and they display potent inhibition against some marine phytoplankton species.

关键词： Trichoderma Diketopiperazine Sesquiterpene Cyclonerane

Mechanisms of autophagy function and regulation in plant growth,development,and response to abiotic stress

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The Crop Journal 2023年第6期11卷 1611-1625页

作者： Yongbo Li Xiangmin Xu Guang Qi Dezhou Cui Chen Huang Xinxia Sui Genying Li Qingqi Fan Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina yantai University Yantai 264005ShandongChina

Autophagy is an evolutionarily conserved degradation pathway of lysosomes(in mammals)and vacuoles(in yeasts and plants)from lower yeasts to higher *** wraps unwanted organelles and damaged proteins in a double-membrane structure to transport them to vacuoles for degradation and *** plants,autophagy functions in adaptation to the environment and maintenance of growth and *** review systematically describes the autophagy process,biological functions,and regulatory mechanisms occurring during plant growth and development and in response to abiotic *** provides a basis for further theoretical research and guidance of agricultural production.

关键词： Autophagy Function Mechanism Development Abiotic stresses