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Identification of predictive factors for post-transarterial chemoembolization liver failure in hepatocellular carcinoma patients: A retrospective study

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World Journal of Clinical Cases 2022年第24期10卷 8535-8546页

作者： Min Yuan Tian-You Chen Xiao-Rong Chen Yun-Fei Lu Jia Shi Wen-Si Zhang Chen Ye Bo-Zong Tang Zong-Guo Yang Department of Interventional Medicine Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508China Department of Integrative Medicine Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508China Department of Internal Medicine of Traditional Chinese Medicine Shanghai East HospitalTongji University School of MedicineShanghai 201200China

BACKGROUND Post-transarterial chemoembolization(TACE)liver failure occurs frequently in hepatocellular carcinoma(HCC)*** identification of predictors for post-TACE liver failure is of great importance for clinical decision-making in this *** To investigate the occurrence rate and predictive factors of post-TACE liver failure in this retrospective study to provide clues for decision-making regarding TACE procedures in HCC *** The clinical records of HCC patients treated with TACE therapy were *** clinical characteristics and laboratory parameters of these patients were *** models were used to identify candidates to predict post-TACE liver *** A total of 199 HCC patients were enrolled in this study,and 70 patients(35.2%)developed post-TACE liver *** and multivariate logistic models indicated that microspheres plus gelatin embolization and main tumor size>5 cm were risk predictors for post-TACE liver failure[odds ratio(OR):4.4,95%confidence interval(CI):1.2-16.3,P=0.027;OR:2.3,95%CI:1.05-5.3,P=0.039,respectively].Conversely,HCC patients who underwent tumor resection surgery before the TACE procedure had a lower risk for post-TACE liver failure(OR:0.4,95%CI:0.2-0.95,P=0.039).CONCLUSION Microspheres plus gelatin embolization and main tumor size might be risk factors for post-TACE liver failure in HCC patients,while prior tumor resection could be a favorable factor reducing the risk of post-TACE liver failure.

关键词： Transarterial chemoembolization Liver failure Hepatocellular carcinoma Embolization Tumor size

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ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover

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Protein & Cell 2023年第9期14卷 653-667页

作者： Maomao Pu Wenhui Zheng Hongtao Zhang Wei Wan Chao Peng Xuebo Chen xinchang Liu Zizhen Xu Tianhua Zhou Qiming Sun Dante Neculai Wei Liu Metabolic Medicine Center International Institutes of Medicinethe Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322000China Joint Institute of Genetics and Genomics Medicine between Zhejiang University and University of Toronto Hangzhou 310058China National Center for Protein Science Shanghai Institute of Biochemistry and Cell BiologyShanghai Institutes of Biological SciencesChinese Academy of SciencesShanghai200031China

Lipophagy,the selective engulfment of lipid droplets(LDs)by autophagosomes for lysosomal degradation,is critical to lipid and energy *** we show that the lipid transfer protein ORP8 is located on LDs and mediates the encapsulation of LDs by autophagosomal *** function of oRP8 is independent of its lipid transporter activity and is achieved through direct interaction with phagophore-anchored LC3/*** lipophagy induction,ORP8 has increased localization on LDs and is phosphorylated by AMPK,thereby enhancing its affinity for LC3/*** of ORP8 or interruption of ORP8-LC3/GABARAP interaction results in accumulation of LDs and increased intracellular *** of ORP8 alleviates LD and triglyceride deposition in the liver of ob/ob mice,and Osbpl8 mice exhibit liver lipid clearance *** results suggest that ORP8 is a lipophagy receptor that plays a key role in cellular lipid metabolism.

关键词： ORP8 lipophagy lipid autophagy

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Standard:Human intestinal cancer organoids

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Cell Regeneration 2023年第1期12卷 193-198页

作者： Hanqing Lin Yalong Wang Chunyan Cheng Yuxin Qian Jie Hao Zhen Zhang Weiqi Sheng Linhong Song Chu‑Xia Deng Bing Zhao Jiani Cao Lei Wang Liu Wang Lingmin Liang Wenli Kelly Chen Chunping Yu Zhijian Sun Yingying Yang Changlin Wang Yong Zhang Qiyuan Li Ka Li Aijin Ma Tongbiao Zhao Ye-Guang Chen Guoqiang Hua D1Med Technology(Shanghai)Inc Shanghai 201802China The State Key Laboratory of Membrane Biology Tsinghua‑Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou Laboratory Guangzhou 510005China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China Guangzhou Hua Yi Regeneration Technology Co. LtdHuangpu DistrictGuangzhou 510700China National Stem Cell Resource Center State Key Laboratory of Stem Cell and Reproductive BiologyInstitute for Stem Cell and RegenerationInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China Institute of Clinical Science Zhongshan HospitalFudan UniversityShanghai 200433China Department of Radiation Oncology and Cancer Institute Fudan University Shanghai Cancer Center Fudan UniversityShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Key Laboratory of Radiation Oncology Shanghai 200032China Department of Pathology Fudan University Shanghai Cancer CenterShanghai 200032China Sichuan Academy of Medical Sciences&Sichuan Provincial People’s hospital Chengdu 610072China Cancer Centre Faculty of Health SciencesUniversity of MacaoMacao 999078SARChina State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 200438China China Innovation Center of Roche Li Shi Zhen RoadPudongShanghai 201203China Eli Lilly and Company PudongShanghai 201203China K2 Oncology Co. LtdKeChuang StreetBeijing 100176China Qilu Pharmaceutical Co. LtdJinan 250104China China National Institute of Standardization Beijing 100191China Chinese Society for Stem Cell Research Shanghai 200032China HHLIFE Co. IncShenzhen 518040China China National GeneBank Shenzhen 518000China Beijing Technology and Business University Beijing 100048China School of Bas

Intestinal cancer is one of the most frequent and lethal types of *** intestinal cancer using organoids has emerged in the last *** intestinal cancer organoids are physiologically relevant in vitro models,which provides an unprecedented opportunity for fundamental and applied research in colorectal cancer.“Human intestinal cancer organoids”is the first set of guidelines on human intestinal organoids in China,jointly drafted and agreed by the experts from the Chinese Society for Cell Biology and its branch society:the Chinese Society for Stem Cell *** standard specifies terms and definitions,technical requirements,test methods for human intestinal cancer organoids,which apply to the production and quality control during the process of manufacturing and testing of human intestinal cancer *** was released by the Chinese Society for Cell Biology on 24 September *** hope that the publication of this standard will guide institutional establishment,acceptance and execution of proper practocal protocols,and accelerate the international standardization of human intestinal cancer organoids for clinical development and therapeutic applications.

关键词： Human jointly apply

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Does novel oral anticoagulant improve anticoagulation for non-valvular atrial fibrillation associated stroke: An inpatient registration study in Shanghai

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Chronic Diseases and Translational Medicine 2015年第4期1卷 -页

作者： Feng-Di Liu Rong Zhao Xue-Mei Wang Shuo Wang Xiao-Lei Shen Xiao-Xiao Tao Bo Zheng Department of Neurology and Jiuyuan Municipal Stroke Center Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China

Objective:To summarize the use rate,safety,efficacy of antithrombotics in stroke/transient ischemic attack (TIA) prevention,and reasons for not using dabigatran etexilate (DE) in Shanghai,***:Non-valvular atrial fibrillation (NVAF)-associated stroke patients were prospectively registered as an electronic *** rate of antithrombofics and reasons for not using DE were extracted during ***' baseline characteristics,recurrent ischemic stroke/TIA events and bleeding complications were ***:From April 2012 to August 2014,110 inpatients with NVAF-associated stroke were studied in our *** was diagnosed by 12-lead electrocardiogram,24 h Holter and ***:Before introduction of DE (April 2013),use rates of warfarin and antiplatelets were 28.9％ (11/38) and 60.5％ (23/38)respectively; after that,use rates of warfarin,DE,and antiplatelets were 20.8％ (15/72),12.5％ (9/72),and 43.1％ (31/72).The DE did not improve use of anticoagulants (P =0.639).There were 19 (17.3％) recurrent ischemic stroke events up to October 2015; two (9.5％) in the non-user group,10 (18.5％) in the antiplatelet group,and seven (20.0％) in the anticoagulants group (P =0.570).Furthermore,recurrence rates were similar between the DE group (20.0％) and the Warfarin group (20.0％,P =1.000).The most common reason for not using DE was financial concerns (61.0％),followed by inconvenience to purchase (14.0％) and hemorrhage concerns (11.0％).Two patients using warfarin found fecal occult blood so they stopped warfarin and began to use antiplatelet *** bleeding event occurred in the other *** one patient had side effects (dyspepsia and gastroesophageal reflux)from ***:The use rate of either DE or warfarin in Shanghai was low; DE had not improved anticoagulation therapy for NVAF patients in Shanghai mainly because DE had not been covered by health insurance.

关键词： Dabigatran etexilate Novel oral anticoagulant Cardiogenic cerebral embolism Non-valvular atrial fibrillation Real-world study

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Standard:Human intestinal organoids

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Cell Regeneration 2023年第1期12卷 199-203页

作者： Yalong Wang Hanqing Lin Lianzheng Zhao Fan Hong Jie Hao Zhen Zhang Weiqi Sheng Linhong Song Chu‑Xia Deng Bing Zhao Jiani Cao Lei Wang Liu Wang Lingmin Liang Wenli Kelly Chen Chunping Yu Zhijian Sun Yingying Yang Changlin Wang Yong Zhang Qiyuan Li Ka Li Aijin Ma Tongbiao Zhao Guoqiang Hua Ye-Guang Chen The State Key Laboratory of Membrane Biology Tsinghua‑Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou Laboratory Guangzhou 510005China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China Guangzhou Hua Yi Regeneration Technology Co. LtdHuangpu DistrictGuangzhou 510700China D1Med Technology(Shanghai)Inc Shanghai 201802China State Key Laboratory of Stem Cell and Reproductive Biology Institute for Stem Cell and RegenerationInstitute of ZoologyNational Stem Cell Resource CenterChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China Institute of Clinical Science Zhongshan HospitalFudan UniversityShanghai 200433China Department of Radiation Oncology and Cancer Institute Fudan University Shanghai Cancer Center Fudan UniversityShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Key Laboratory of Radiation Oncology Shanghai 200032China Department of Pathology Fudan University Shanghai Cancer CenterShanghai 200032China Sichuan Academy of Medical Sciences&Sichuan Provincial People’s hospital Chengdu 610072China Cancer Centre Faculty of Health SciencesUniversity of MacaoMacao 999078SARChina State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 200438China China Innovation Center of Roche Li Shi Zhen RoadPudongShanghai 201203China Eli Lilly and Company PudongShanghai 201203China K2 Oncology Co. LtdKeChuang StreetBeijing 100176China Qilu Pharmaceutical Co. LtdJinan 250104China China National Institute of Standardization Beijing 100191China Chinese Society for Stem Cell Research Shanghai 200032China HHLIFE Co. IncShenzhen 518040China China National GeneBank Shenzhen 518000China Beijing Technology and Business University Beijing 100048China School of Bas

Organoids have attracted great interest for disease modelling,drug discovery and development,and tissue growth and homeostasis ***,lack of standards for quality control has become a prominent obstacle to limit their translation into clinic and other applications.“Human intestinal organoids”is the first guideline on human intestinal organoids in China,jointly drafted and agreed by the experts from the Chinese Society for Cell Biology and its branch society:the Chinese Society for Stem Cell *** standard specifies terms and definitions,technical requirements,test methods,inspection rules for human intestinal organoids,which is applicable to quality control during the process of manufacturing and testing of human intestinal *** was originally released by the Chinese Society for Cell Biology on 24 September *** hope that the publication of this standard will guide institutional establishment,acceptance and execution of proper practical protocols and accelerate the international standardization of human intestinal organoids for applications.

关键词： Human jointly branch

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The microRNAs expression changes rapidly in mice lung tissue during lipopolysaccharide-induced acute lung injury

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Chinese Medical Journal 2013年第1期126卷 181-183页

作者： GUO Zhong-liang REN Tao XU Lin ZHANG Lu YIN Qin WANG Jun-chen LIANG Yong-jie Department of Respiratory MedicineEasthospital Tongji University School of Medicine Shanghai 200120China Department of Pathology East hospital Tongji University School of Medicine Shanghai 200120China Department of Immunology Zunyi Medical College ZuiyiGuizhou 563003 China

MicroRNAs （miRNAs） are a novel class of-22-nt, .non-coding, short single-stranded RNAs producedfrom endogenous genes by the sequential action of the RNase III enzymes Drosha and Dicer. Increasing evidence shows that miRNAs play a critical role in regulating inflammatory and innate immunity.1,2 However, whether miRNAs are involved in the altered function of innate immune cells and ultimately contribute to the development of acute lung injury （ALl） remains largely unknown. It is worth studying the expression profiles of miRNAs in mouse lungs stimulated by lipopolysaccharide （LPS）, which may be very important in the regulation of inflammatory mediators during ALI. In the present study, we investigated the expression profiles of miRNAs in LPS-induced ALI in mice.

关键词： acute lung injury microRNA lipopolysaccharide pathogenesis

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5-mRNA-based prognostic signature of survival in lung adenocarcinoma

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World Journal of Clinical Oncology 2023年第1期14卷 27-39页

作者： Qian-Lin Xia Xiao-Meng He Yan Ma Qiu-Yue Li Yu-Zhen Du Jin Wang Laboratory Medicine Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200233China Scientific Research Center Shanghai Public Health Clinical CenterFudan UniversityShanghai 201508China

BACKGROUND Lung adenocarcinoma(LUAD)is the most common non-small-cell lung cancer,with a high incidence and a poor *** To construct effective predictive models to evaluate the prognosis of LUAD *** In this study,we thoroughly mined LUAD genomic data from the Gene Expression Omnibus(GEO)(GSE43458,GSE32863,and GSE27262)and the Cancer Genome Atlas(TCGA)datasets,including 698 LUAD and 172 healthy(or adjacent normal)lung tissue *** regression and LASSO regression analyses were used to screen differentially expressed genes(DEGs)related to patient prognosis,and multivariate Cox regression analysis was applied to establish the risk score equation and construct the survival prognosis *** operating characteristic curve and Kaplan-Meier survival analyses with clinically independent prognostic parameters were performed to verify the predictive power of the model and further establish a prognostic *** A total of 380 DEGs were identified in LUAD tissues through GEO and TCGA datasets,and 5 DEGs(TCN1,CENPF,MAOB,CRTAC1 and PLEK2)were screened out by multivariate Cox regression analysis,indicating that the prognostic risk model could be used as an independent prognostic factor(Hazard ratio=1.520,Parea under the curve=0.754,0.737).Combining genetic models and clinical prognostic factors,nomograms can also predict overall survival more *** A 5-mRNA-based model was constructed to predict the prognosis of lung adenocarcinoma,which may provide clinicians with reliable prognostic assessment tools and help clinical treatment decisions.

关键词： Lung adenocarcinoma Differentially expressed genes Prognostic signature Risk score Nomogram

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An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19

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Signal Transduction and Targeted Therapy 2021年第5期6卷 1585-1600页

作者： Yiyue Ge Tingzhong Tian Suling Huang Fangping Wan Jingxin Li Shuya Li Xiaoting Wang Hui Yang Lixiang Hong Nian Wu Enming Yuan Yunan Luo Lili Cheng Chengliang Hu Yipin Lei Hantao Shu Xiaolong Feng Ziyuan Jiang Yunfu Wu Ying Chi Xiling Guo Lunbiao Cui Liang Xiao Zeng Li Chunhao Yang Zehong Miao Ligong Chen Haitao Li Hainian Zeng Dan Zhao Fengcai Zhu Xiaokun Shen Jianyang Zeng Institute for Interdisciplinary Information Sciences Tsinghua UniversityBeijingChina NHC Key laboratory of Emeric Pathogenic Microbiology Jiangsu Provincial Center for Diseases Control and PreventionNanjingJiangsu ProvinceChina Shanghai Institute of Materia Medica Chinese Academy of SciencesShanghaiChina Silexon Al Technology Co. Ltd.NanjingJiangsu ProvinceChina Department of Computer Science University of Illinois at Urbana-ChampaignIllinoisILUSA School of Pharmaceutical Sciences Tsinghua UniversityBeijingChina School of Electronic Information and Communications Huazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Automation Tsinghua UniversityBeijingChina Inner Mongolia Alashan League Organization Establishment Committee Office Electronic Support Center AlashanInner MongoliaChina Convalife(Shanghai)Co. Ltd.ShanghaiChina Advanced Innovation Center for Human Brain Protection Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Beijing Advanced Innovation Center for Structural Biology Tsinghua-Peking Joint Center for Life SciencesDepartment of Basic Medical SciencesSchool of MedicineTsinghua UniversityBeijingChina Center for Global Health Nanjing Medical UniversityNanjingJiangsu ProvinceChina

The global spread of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)requires an urgent need to find effective therapeutics for the treatment of coronavirus disease 2019(COVID-19).In this study,we developed an integrative drug repositioning framework,which fully takes advantage of machine learning and statistical analysis approaches to systematically integrate and mine large-scale knowledge graph,literature and transcriptome data to discover the potential drug candidates against *** in silico screening followed by wet-lab validation indicated that a poly-ADP-ribose polymerase 1(PARP1)inhibitor,CVL218,currently in Phase I clinical trial,may be repurposed to treat *** in vitro assays revealed that CVL218 can exhibit effective inhibitory activity against SARS-CoV-2 replication without obvious cytopathic *** addition,we showed that CVL218 can interact with the nucleocapsid(N)protein of SARS-CoV-2 and is able to suppress the LPS-induced production of several inflammatory cytokines that are highly relevant to the prevention of immunopathology induced by SARS-CoV-2 infection.

关键词： prevention respiratory acute

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An erratum on “Application of three-dimensional printing in interventional medicine” [J Interv Med(February 2020) 1–16]

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Journal of Interventional Medicine 2020年第3期3卷 156-156页

作者： Geng Zhou Weidong Liu Yi Zhang Wenquan Gu Minghua Li Chuan Lu Ran Zhou Yichen Che Haitao Lu Yueqi Zhu Gaojun Teng Yongde Cheng Center of Interventional Radiology&Vascular Surgery Department of RadiologyZhongda HospitalMedical SchoolSoutheast UniversityNanjing210000China Shanghai Punan hospital International Medical Department Shanghai200125China Department of Radiology Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200233China School of Radiology Taishan Medical CollegeShandong271016China Department of Ultrasonography Zaozhuang Mining Group Central HospitalZaozhuangShandong277800China Department of Ultrasonography Maternity&Child Health Hospital of Mudan DistrictHezeShandong274000China Department of Radiology No.85Hospital of PLAShanghai200052China

After the publication of this work,1 the authors noticed and confirmed that the Funding Information was mistakenly omitted from the *** statement“This study was supported by grants from the National Natural Science Foundation of China,grant no.81370041,81471760,81671655,the Outstanding Clinical Discipline Project of Shanghai pudong,grant *** authors declare that they have no conflicts of interest.”should be included in the Funding information section of the paper which is *** apologize for the error.

关键词： printing statement fir

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A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology

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Infectious Diseases of Poverty 2023年第4期12卷 43-53页

作者： Qunzheng Mu Xin Zhao Fengfeng Li Wenyu Li Xinxin Zhou xinchang Lun Yiguan Wang Dongdong Hua Qiyong Liu Di Xiao Fengxia Meng National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206People’s Republic of China Weifang No.2 People’s hospital Weifang261000ShandongPeople’s Republic of China Weifang Medical College Weifang261000ShandongPeople’s Republic of China Beijing Daxing District Center for Disease Control and Prevention Beijing102600BeijingPeople’s Republic of China

Background The current prevention and control strategy forAedes albopictus heavily relies on comprehensive management, such as environmental management and chemical control. However, the wide application of pyrethroids has facilitated the development of insecticide resistance, primarily via mutations in the voltage-gated sodium channel (VGSC) gene. This study aims to develop a novel strategy for detecting mutations in the VGSC gene inAe. albopictus using multiplex PCR-mass spectrometry (MPCR-MS) minisequencing *** We established a new strategy for detecting mutations in the VGSC gene inAe. albopictus using MPCR-MS minisequencing technology. MPCR amplification and mass probe extension (MPE) were first used, followed by single nucleotide polymorphism (SNP) typing mass spectrometry, which allows the simultaneous detection of multiple mutation sites of the VGSC gene in 96 samples ofAe. albopictus. A total of 70 wild-collectedAe. albopictus were used to evaluate the performance of the method by comparing it with other *** Three target sites (1016, 1532, 1534) in the VGSC gene can be detected simultaneously by double PCR amplification combined with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, achieving a detection limit of 20 fg/μl. We applied this method to 70 wild-collectedAe. albopictus, and the obtained genotypes were consistent with the routine sequencing results, suggesting the accuracy of our *** MPCR-MS minisequencing technology provides a sensitive and high-throughput approach toAe. albopictus VGSC gene mutation screening. Compared with conventional sequencing, this method is economical and time-saving. It is of great value for insecticide resistance surveillance in areas with a high risk of vector-borne disease.

关键词： Aedes albopictus VGSC gene Mutation Single nucleotide polymorphisms Multiplex polymerase chain reaction-mass spectrometry mini-sequencing

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