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RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

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Journal of Animal Science and Biotechnology 2023年第3期14卷 917-935页

作者： xinhao Ma xinran Yang Dianqi Zhang Wenzhen Zhang xiaoyu Wang Kuncheng xie Jie He Chugang Mei Linsen Zan College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi 712100People’s Republic of China xi’an dairy cow breeding center Xi’an Agriculture and Rural BureauXi’anShaanxi 712100People’s Republic of China National Beef Cattle Improvement center Northwest A&F UniversityYanglingShaanxi 712100People’s Republic of China

Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including *** on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains *** research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular *** In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel *** on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory *** knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular ***,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling *** This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular *** plays a crucial role in adipogenesis through the ERK1/2 signaling *** results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research.

关键词： Adipogenesis BIANCR Bovine Intramuscular adipocyte LncRNAs

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Current research progresses on calf rearing and nutrition in China

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Journal of Integrative Agriculture 2017年第12期16卷 2805-2814页

作者： DIAO Qi-yu ZhAng Rong Tu Yan Feed Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology Ministry of Agriculture/Beijing Key Laboratory of Dairy Cow Nutrition Beijing 100081 P.R.China

Calves are the reserve forces for dairy cattle. Scientific rearing strategy of calves is the basis of efficient cattle breeding. However, many problems exist in current rearing systems of calves and restrict the sustainable development of dairy cattle in China. The absence of basic research is the most highlighted problem among them. Recent researches on growth performance, nutrient digestibility, blood biochemical indices and rumen development in calves were summarized in this paper. Additionally, the optimal levels of energy and protein in milk replacer and starter diets for calves were indicated, and limiting amino acids for calves fed different diets were discussed. A variety of additives, such as acidifiers, probiotics and polysaccharides, are regarded as promising alternatives to antibiotics to reduce disease in calves. Dietary supplementations of these additives have positive effects on growth and health of calves. However, studies on the nutrition of vitamins and minerals in calves have been seldom done, and deserve our further researches. To sum up, the postnatal period is one of the most critical “windows” for rumen manipulation and epigenetic regulation. Any changes from environments, especially early nutrition, may produce long-term effects on growth, health and milk yields in adult cattle.

关键词： calf rearing growth physiology nutrition additives

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CLC treatment significantly improves the fertilization capacity of vitrified bovine oocytes by protecting fertilization protetin JUNO

CLC treatment significantly improves the fertilization capac...

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中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者： Tong Hao Sha Yang Hai-Sheng Hao Wei-Hua Du Yun-Wei Pang Shan-Jiang Zhao Hui-Ying Zou Hua-Bin Zhu Wei-LI Yu Shu-Jing Li Xue-Ming Zhao Embryo Biotechnology and Reproduction Laboratory Institute of Animal SciencesChinese Academy of Agricultural Sciences Shijiazhuang Tianquan Elite dairy Lt.D. Hebei Provincial dairy cow breeding Engineering Technology Research center Hebei Cattle Industry Technology Research Institute

(Objective) Oocyte cryopreservation is to store oocyte in liquid nitrogen at-196℃ to reduce cell metabolism and extend storage *** experiments shows that vitrification significantly reduced the fertilization capacity,which seriously restricted the wide application of vitrified *** has proven that the JUNO protein plays vital role in the fertilization of mammalian ***,little information in the effects of vitrification on the JUNO protein of bovine oocytes,and even less about the procedure to protect it.(Method) Therefore,this study was design to investigate the effect of vitrification on the JUNO expression level in bovine *** MII oocytes were treated with various concentration of cholesterol-loaded methyl-β-cyclodextr(CLC)(0 mM,10 mM,15 mM,20 mM) for 45 min before vitrification,and methyl-β-cyclodextrin(MβCD)(0 mM,2.25 mM,4.25 mM,6.25 mM) for 45 min after *** the expression level and function of JUNO,cholesterol level in membrane,the externalization of phosphatidylserine,sperm binding capacity and the developmental ability of vitrification bovine oocytes were examined.(Result) Our results showed vitrification significantly decreased the JUNO protein level,cholesterol level,sperm-egg fusion ability,development ability of bovine *** vitrification significantly increased the promoter methylation level of JUNO gene and apoptosis level of bovine oocyte.15 mM CLC+4.25 mM MβCD treatment can significantly improve the cholesterol level in bovine oocyte, and then increase sperm binding and development ability of vitrified bovine oocytes by increasing JUNO express level and membrane integrity.(Conclusion) These findings will illustrate the reason that decreased fertilization ability of oocytes after vitrified,provide a reliable approach to protect the fertilization ability of vitrified bovine oocytes,which contribute to the wild application of vitrified oocytes in future.

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Endocrine and ovarian responses in water buffalo cows immunized against inhibin and subjected to the Ovsynch protocol

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Journal of Integrative Agriculture 2015年第9期14卷 1827-1837页

作者： Abdalla Bahareldin-Ali QIN Guang-sheng GUO Ri-hong Anastasia Tsigkou TAN Zheng-zhun HUANG Jian LI Hui LI Hui SHI Zhen-dan College of Animal Sciences South China Agricultural University Guangzhou 510642 P.R. China Key Laboratory of Buffalo Genetics Breeding and Reproduction Technology Guangxi Buffalo Research Institute Nanning 530000 P.R.China Department of Biological Sciences Xi'an Jiaotong-Liverpool University Suzhou 215123 P.R. China Laboratory of Animal breeding and Reproduction Institute of Animal Science Jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R.China

The aim of this study was to investigate the feasibility of stimulating ovarian fol icle development in order to improve fertility in water buffalo cows by immunization against inhibin. The experiment was carried out in early summer (May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized (n=11) and control (n=13) groups. Each immunized cow was administered with a 2-mL immunogen of mineral oil adjuvant containing 2 mg of recombinant inhibinα-subunit fusion protein. The controls were treated with the adjuvant only. Al animals received Ovsynch protocol treatment, starting on the day of the antigen administration, and they were artiifcial y inseminated upon behavioral estrus. As a result, al of the immunized buffaloes generated antibodies against inhibin during the experimental period and had higher plasma concentrations of fol icle-stimulating hormone (FSH), activin, and estradiol (E2) related to estrous expression. A higher proportion of immunized animals expressed estrus behavior than did the controls (72%vs. 30%, Pcows ovulated in the immunized group than in the control group (91%(10/11) vs. 54%(7/13), respectively;Pcows had slightly larger corpus luteum (CL) than the controls 9 days after ovulation and signiifcantly higher (P0.05). These results demonstrate that immunization against inhibin, coupled with the treatment with the O

关键词： inhibin immunization ovarian responses luteal function hormonal patterns conception rate water buffalo cows

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Cortisol levels in skimmed milk during the first 22 weeks of lactation and response to short-term metabolic stress and lameness in dairy cows

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Journal of Animal Science and Biotechnology 2015年第4期6卷 485-491页

作者： Katharina Gellrich Tanja Sigl Heinrich H.D.Meyer Steffi Wiedemann Physiology Weihenstephan Technische Universitaet Muenchen Animal Health Institute of Animal Breeding and HusbandryKiel University

Background： Cortisol is secreted into blood in reaction to acute stress, but also in phases of diminished feed intake and changed animal behavior. As cows do not always show clear signs of discomfort, reliable diagnostic markers could be used to provide information regarding individual cows＇ distress. The objective of this study was to establish an ether free immunoassay for the detection of cortisol and to determine values during the first 22 weeks of lactation. Furthermore, the response in milk cortisol levels was assessed during times of metabolic stress and pain associated symptoms of lameness. Methods： Milk yield and composition, blood serum glucose, NEFA and BHBA as well as milk cortisol were determined in 24 multiparous Holstein-Friesian cows over the course of the first 22 weeks of lactation. Animals were further checked for signs of clinical diseases on a daily basis. Two feed restrictions over three days （FR; 70 % of precious ad libitum intake） were performed during the 4th wk and the 21st wk, respectively. An ELISA for cortisol measurement in easily accessible bovine skimmed milk was established and applied. Results： On the last day of FR in early lactation, a reduction in milk yield and changes in serum metabolites compared to respective previous values were detected. The FR in mid-lactation resulted in no changes in milk production and serum metabolites. Milk cortisol was highest during first wk of lactation and remained on comparable levels thereafter. Milk yield and composition were not influenced by FR. Lameness resulted in enhanced milk cortisol levels. Conclusion： Milk cortisol could be used as an indicator of painful symptoms such as lameness. Higher values of milk cortisol levels during first wk of lactation should be taken into account for interpretation.

关键词： Cortisol dairy cow ELISA Feed restriction Milk

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Variance components and correlations of female fertility traits in Chinese Holstein population

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Journal of Animal Science and Biotechnology 2017年第4期8卷 833-841页

作者： Aoxing Liu Mogens Sandø Lund Yachun Wang Gang Guo Ganghui Dong Per Madsen Guosheng Su Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture of China National Engineering Laboratory of Animal BreedingCollege of Animal Science and Technology China Agricultural UniversityBeijing 100193 China center for Quantitative Genetics and Genomics Department of Molecular Biology and Genetics Aarhus University 8830 TjeleDenmark. Beijing Sunlon Livestock Development Co. Ltd Beijing 100176 China

Background: The objective of the present study was to estimate(co)variance components of female fertility traits in Chinese Holsteins, considering fertility traits in different parities as different traits. Data on 88,647 females with 215,632 records(parities) were collected during 2000 to 2014 from 32 herds in the Sanyuan Lvhe dairy Cattle center, Beijing, China. The analyzed female fertility traits included interval from calving to first insemination, interval from first to last insemination, days open, conception rate at first insemination, number of inseminations per conception and non-return rates within 56 days after first ***: The descriptive statistics showed that the average fertility of heifers was superior to that of cows. Moreover,the genetic correlations between the performances of a trait in heifers and in cows were all moderate to high but far from one, which suggested that the performances of a trait in heifers and cows should be considered as different but genetically correlated traits in genetic evaluations. On the other hand, genetic correlations between performances of a trait in different parities of cows were greater than 0.87, with only a few exceptions, but variances were not homogeneous across parities for some traits. The estimated heritabilities of female fertility traits were low; all were below 0.049(except for interval from calving to first insemination). Additionally, the heritabilities of the heifer interval traits were lower than those of the corresponding cow interval traits. Moreover, the heritabilities of the interval traits were higher than those of the threshold traits when measuring similar fertility functions. In general, estimated genetic correlations between traits were highly consistent with the biological categories of the female fertility ***: Interval from calving to first insemination, interval from first to last insemination and non-return rates within 56 days after first insemination ar

关键词： Chinese Holsteins Female fertility Genetic correlation Heritability

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Predicting nitrogen use efficiency,nitrogen loss and dry matter intake of individual dairy cows in late lactation by including mid‑infrared spectra of milk samples

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1287-1299页

作者： Rui Shi Wenqi Lou Bart Ducro Aart van der Linden Han A.Mulder Simon J.Oosting Shengli Li Yachun Wang Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture of ChinaNational Engineering Laboratory of Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Wageningen University&Research Animal breeding and Genomics P.O.Box 3386700 AH Wageningenthe Netherlands Animal Production System Group Wageningen University&ResearchP.O.Box 3386700 AH Wageningenthe Netherlands

Background Nitrate leaching to groundwater and surface water and ammonia volatilization from dairy farms have negative impacts on the ***,the increasing demand for dairy products will result in more pollution if N losses are not ***,a more efficient,and environmentally friendly production system is needed,in which nitrogen use efficiency(NUE)of dairy cows plays a key *** genetically improve NUE,extensively recorded and cost-effective proxies are essential,which can be obtained by including mid-infrared(MIR)spectra of milk in prediction models for *** study aimed to develop and validate the best prediction model of NUE,nitrogen loss(NL)and dry matter intake(DMI)for individual dairy cows in *** A total of 86 lactating Chinese Holstein cows were used in this *** data editing,704 records were obtained for calibration and *** prediction models with three different machine learning algorithms and three kinds of pre-processed MIR spectra were developed for each *** showed that the coefficient of determination(R2)of the best model in within-herd validation was 0.66 for NUE,0.58 for NL and 0.63 for *** external validation,reasonable prediction results were only observed for NUE,with R2 ranging from 0.58 to 0.63,while the R2 of the other two traits was below *** infrared waves from 973.54 to 988.46 cm−1 and daily milk yield were the most important variables for *** The results showed that individual NUE can be predicted with a moderate accuracy in both within-herd and external *** model of NUE could be used for the datasets that are similar to the calibration *** prediction models for NL and 3-day moving average of DMI(DMI_a)generated lower accuracies in within-herd *** also indicated that information of MIR spectra variables increased the predictive ability of ***,pre-processed MIR spectra do not result in higher acc

关键词： dairy cow Environment Mid-infrared spectra Nitrogen use efficiency Prediction

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Predicting the excretion of feces,urine and nitrogen using support vector regression:A case study with Holstein dry cows

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International Journal of Agricultural and Biological Engineering 2020年第2期13卷 48-56页

作者： Qiang Fu Weizheng Shen xiaoli Wei Yanling Yin Ping Zheng Yonggen Zhang Zhongbin Su Chunjiang Zhao College of Electrical and Information Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Pig-breeding Facilities Engineering Ministry of AgricultureHarbin 150030China College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030China National Engineering Research center for Information Technology in Agriculture Beijing 100097China

Predicting the excretion of feces,urine and nitrogen(N)from dairy cows is an effective way to prevent and control the environmental pollution caused by scaled *** traditional prediction methods such as pollutant generation coefficient(PGC)and mathematical model based on linear regression(LR)may be limited by prediction range and regression function assumption,and sometimes may deviate from the actual *** order to solve these problems,the support vector regression(SVR)was applied for predicting the cows'feces,urine and N excretions,taking Holstein dry cows as a case *** is a typical non-parametric machine learning model that does not require any specific assumptions about the regression function in advance and only by learning the training sample data,and also it can fit the function closest to the actual in most *** evaluate prediction accuracy effectively,the SVR technique was compared with the LR and radial basis function artificial neural network(RBF-ANN)methods,using the required sample data obtained from actual feeding *** prediction results indicate that the proposed technique is superior to the other two conventional(especially LR)methods in predicting the main indicators of feces,urine,and N excretions of Holstein dry cows.

关键词： cow farming pollution feces/urine excretion prediction nitrogen excretion prediction non-parametric model SVR technique

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Quality Analysis of dairy Herd Improvement Data from Henan Province

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Agricultural Science & Technology 2017年第1期18卷 151-155,188页

作者： Zhen ZHANG xiaoli REN Lei YAN Yuefei YAN Fanjun GENG Yanqin SUN Henan dairy Improvement center Zhengzhou 450046 China Henan Dingyuan Cattle breeding Co. Ltd. Zhengzhou 450008 China Henan Animal Health Supervision Zhengzhou 450008 China

The dairy herd improvement data from Henan Province were analyzed statistically to establish screening criteria for relevant data, thereby laying a foundation for genetic evaluation of dairy cows. With the 2 152 451 test-day records about 155 893 Chinese Holstein dairy cows collected by the Henan dairy Herd Improvement center from January 2008 to April 2016, the dynamics of test times during a complete lactation, test interval during a complete lactation, days in milk （DIM） of first test-day record, daughter descendant number and herd number of bull, age at first calving and pedigree integrity rate among different years and different herd sizes were analyzed by MEANS order of SAS 9.4. In addition, the data that were applicable to genetic evaluation were screened by SQL program. The results showed that during 2008-2015, the number of cow individuals participating in DHI in Henan Province increased from 7 379 to 93 706; the test-day milk yield increased from 19.91 to 24.05 kg; the somatic cell count reduced from 411.09×10^3 to 277.08×10^3 cells/ml; the percentage of cows with DIM ranging from 5-305 d reached 70.92%; the average test times increased from 3.20 to 6.31 times; the test interval decreased from 70.22 to 33.83 d; the dairy cows with age at first calving of 25 months were dominant, accounting for 12.57%; the bulls whose daughter descendant number was 20 or more and the daughters were distributed in 10 or more farms accounted for 6.05%; the one-generation pedigree integrity rate was 82.54%; the percentage of data that could be used for genetic evaluation was screened as 20.67%, which was lower than the results of other similar studies.

关键词： dairy herd improvement data quality Test times Test interval DIM of first test-day record Daughter descendant number and herd number of bull age at first calving Pedigree integrity rate

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Prediction of the diet nutrients digestibility of dairy cows using Gaussian process regression

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Information Processing in Agriculture 2019年第3期6卷 396-406页

作者： Qiang Fu Weizheng Shen xiaoli Wei Ping Zheng Hangshu xin Chunjiang Zhao College of Electrical and Information Northeast Agricultural UniversityHarbin 150030PR China Key Laboratory of Pig-breeding Facilities Engineering Ministry of AgricultureHarbin 150030PR China College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030PR China National Engineering Research center for Information Technology in Agriculture Beijing 100097PR China

In order to effectively evaluate the diet nutritional value of dairy cows,it is essential to accurately predict the diet nutrients digestibility(DND).Conventional predicting DND methods are usually based on the least squares linear regression analysis(LS-LRA),which often relies on a large amount of training samples to accomplish reliable ***,in real-world applications,it is often extremely difficult,costly and time-consuming to obtain a large number of measured samples,especially for the DND prediction of dairy *** paper applies a Gaussian process regression(GPR)technique to predict the DND indicators of dairy cows in small *** evaluate prediction accuracy effectively,we compared the GPR technique with the LS-LRA,radial basis function artificial neural network(RBF-ANN),support vector regression(SVR)and least squares support vector regression(LS-SVR)methods,using the required sample data obtained from actual digestion *** prediction results indicate that the GPR technique is superior to other conventional methods(especially the LS-LRA method)in predicting the main DND indicators of dairy cows such as dry matter digestibility(DMD),organic matter digestibility(OMD),neutral detergent fiber(NDFD),acid detergent fiber(ADFD),and crude protein digestibility(CPD).It is worth mentioning that the developed GPR-based prediction technique is more suitable for the prediction problems with small samples,which is often the case in the prediction of DND indicators of dairy cows,and then more coincide with actual needs.

关键词： Precision feeding dairy cow diet evaluation Diet nutrients digestibility Small sample prediction GPR algorithm

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