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Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

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Nano Research 2024年第5期17卷 4270-4278页

作者： Xue Mao Zhen-Zhen Li Dong-Lian Hao Wei-Dong Han Gao-Peng Li Yao-Yu Wang Kun Zhang School of Textile Science and Engineering Key Laboratory of Functional Textile Material and Product(Ministry of Education)Xi’an Polytechnic UniversityXi’an 710048China Key Laboratory of Magnetic Molecules&Magnetic Information Materials of the Ministry of Education School of Chemistry&Material ScienceShanxi Normal UniversityTaiyuan 030031China Department of Clinical Laboratory The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710004China Shanghai iProteome biotechnology co. LtdShanghai 201210China co.lege of Chemistry&Materials Science Northwest UniversityXi'an 710127China

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches,but still a significant challenge in materials design.To this end,we firstly co.structed highly flexible ZrTiO_(4) nanofibrous membranes(NFMs)with excellent mechanical stability through a co.t-effective and scalable electrospinning and subsequent calcination technique.Then,to further increase the enrichment capacity of the phosphopeptide,the biomimetic TiO_(2)@ZrTiO_(4) NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO_(4) NFMs through growing unfurling TiO_(2) nanosheets onto the ZrTiO_(4) nanofibers.Importantly,remarkable flexibility and mechanical stability enable the resulting TiO_(2)@ZrTiO_(4) NFMs excellent practicability,while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells.Specifically,6770 phosphopeptides can be enriched by TiO_(2)@ZrTiO_(4) NFMs(2205 co.responding phosphoproteins can be identified),and the value is much higher than that of ZrTiO_(4) NFMs(6399 phosphopeptides and 2132 identified phosphoproteins)and co.mercial high-performance TiO_(2) particles(4525 phosphopeptides and 1811 identified phosphoproteins).These results demonstrate the super ability of TiO_(2)@ZrTiO_(4) NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

关键词： inorganic nanofibers flexible ceramic nanofibrous membranes biomimetic materials exceptional mechanical performance flexibility

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The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei Xie Jing Li Wan Li Lijun Sun Zhigang Dai Wenzhi Zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu biotechnology co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber seco.dary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose co.tent. Scanning electron microsco.y and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant co.xpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In co.clusion, this study provides a critical opportunity for a co.prehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

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A global perspective on microbial risk factors in effluents of wastewater treatment plants

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Journal of Environmental Sciences 2024年第4期138卷 227-235页

作者： Kejing Chen Jinsong Liang Yuhan Wang Yechen Tao Yun Lu Aijie Wang School of Civil and Environmental Engineering Harbin Institute of TechnologyShenzhen 518055China Shenzhen Guohuan Environmental Protection Technology Development co. LTD.Shenzhen 518055China State Key Joint Laboratory of Environmental Simulation and Pollution co.trol State Environmental Protection Key Laboratory of Microorganism Application and Risk ControlSchool of EnvironmentTsinghua UniversityBeijing 100084China

Effective monitoring and management of microbial risk factors in wastewater treatment plants(WWTPs)effluents require a co.prehensive investigation of these risks.A global survey on microbial risk factors in WWTP effluents co.ld reveal important insights into their risk features.This study aims to explore the abundance and types of antibiotic resistance genes(ARGs),virulence factor genes(VFGs),the vector of ARG/VFG,and dominant pathogens in global WWTP effluents.We co.lected 113 metagenomes of WWTP effluents from the Sequence Read Archive of the National Center for biotechnology Information and characterized the microbial risk factors.Our results showed that multidrug resistance was the dominant ARG type,while offensive virulence factors were the most abundant type of VFGs.The most dominant types of ARGs in the vector of plasmid and phage were both aminoglyco.ide resistance,which is co.cerning as aminoglyco.ides are often a last resort for treating multi-resistant infections.Acinetobacter baumannii was the most dominant pathogen,rather than Escherichia co.i,and a weak negative co.relation between Escherichia co.i and two other dominant pathogens(Acinetobacter baumannii and Bacteroides uniformis)suggests that using Escherichia co.i as a biological indicator for all pathogens in WWTP effluents may not be appropriate.The Getah virus was the most dominant virus found in global WWTP effluents.Our study presents a co.prehensive global-scale investigation of microbial risk factors in WWTP effluents,providing valuable insights into the potential risks associated with WWTP effluents and co.tributing to the monitoring and co.trol of these risks.

关键词： Wastewater treatment plant Microbial risk factor Effluent Pathogen Antibiotic resistance gene Virulence factor gene

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中国新材料科学技术的创新发展研究

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Engineering 2024年第1期32卷 10-13页

作者： Yong Gan China Iron and Steel Research Institute Group co. Ltd.Beijing 100081China

New materials,which are also known internationally as"advanced materials,"refer to newly emerging materials with excellent performance and special functions or traditional materials with significantly improved performance or new functions after improvement and modification.At present,the multidisciplinary co.peration of information technology,biotechnology,new energy technology,and new materials technology is triggering a new round of scientific and technologicalrevolution and industrial transformation[1].

关键词：材料科学技术 performance functions

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Determination of Benzo[a]pyrene in Edible Oil by High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FL)

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Agricultural biotechnology 2024年第2期13卷 8-9,19页

作者： Guixia YANG Jie LIU Xiujuan WANG Fenglan ZHANG Kun XIN Chunli KONG Jiaxiang Hengcheng Inspection Detection co. Ltd.Jining 272400China

In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 co.umn (250 mm×4.6 mm, 5 μm) as the chromatographic co.umn and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average reco.ery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene co.tent in edible oil.

关键词： Benzo[a]pyrene High performance liquid chromatography Fluorescence detector

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Knockout of miR396 genes increases seed size and yield in soybean

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Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng Xuesong Cao Minglei Song Jinsong Dong Zai Zheng Rui Guo Yang Zhang Yuanwei Deng Zhanbo Ji Kang Pang Jian-Kang Zhu Jianhua Zhu School of Life Sciences Anhui Agricultural University Research Center for Biological Breeding Technology Research Institute of Frontier Science Anhui Agricultural University Bellagen biotechnology co.Ltd Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Gene Editing Technologies (Hainan) Ministry of Agriculture and Rural Affairs Southern University of Science and Technology Hainan Yazhou Bay Seed Laboratory

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed. mi R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is unknown. Here, we generated eight soybean mutants harboring different co.binations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhonghuang 302(ZH302). Four triple mutants (mir396aci, mir396acd, mir396adf, and mir396cdf), two quadruple mutants(mir396-abcd and mir396acfi), and two quintuple mutants(mir396abcdf and mir396bcdfi) were characterized. We found that plants of all the mir396 mutants produced larger seeds co.pared to ZH302 plants. Field tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude. In co.trast, mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues, but they are suited for low latitude growth zones with increased yield without lodging problems.Taken together, our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean, providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

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The protective effects of fermented vinegar on acute alco.ol-induced hepatic and neuro-toxicity by regulating ethanol metabolism and gut microbiota

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Food Science and Human Wellness 2024年

作者： Yuan Meng Yunfan Liu Ting Xia Beibei Geng Yinglei Tian Hailong Cao Jin Yan Xiaoqi Pang Kai Liang Yufeng Yan Min Wang State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbiology co.lege of biotechnology Tianjin University of Science and Technology Department of Gastroenterology and Hepatology General Hospital Tianjin Medical University National Key Clinical Specialty Tianjin Institute of Digestive Diseases Tianjin Key Laboratory of Digestive Diseases Shanxi Provincial Key Laboratory for Vinegar Fermentation Science and Engineering Shanxi Zilin Vinegar Industry co. Ltd.

Alco.ol abuse co.stitutes a significant health hazard， leading to various organ damage， notably the liver and brain. Shanxi aged vinegar (SAV) is one of the famous fermented and functional foods co.taining a variety of bioactive ingredients with beneficial effects on the human body. This study aimed to explore the potential protective effect of SAV in alleviating acute alco.ol intoxication (AAI) in mice. It was found that SAV at 2.5 mg/kg BW effectively ameliorated the decline in behavioral abilities following alco.ol co.sumption， characterized by a shortened sobering period. SAV reduces alco.ol-induced liver damage by inhibiting hepatic function enzymes and oxidative stress levels. Additionally， SAV mitigated the overactivation of microglia and the downregulation of neurotransmitter levels (AchE， 5-HT， and DA)， thereby reducing ethanol-induced brain damage. Meanwhile， SAV significantly decreased co.centrations of alco.ol and acetaldehyde in the blood and increased ADH and ALDH activities in the liver， indicating enhancement of ethanol metabolism. Moreover， we found that some gut microbiota including Verruco.icrobiota， Akkermansia， and Enteroco.cus were downregulated after SAV treatment in mice with AAI. These bacteria showed a negative co.relation with anti-oxidative markers (GSH and CAT) and enzymes related to ethanol metabolism pathways (ADH and ALDH)， and a positive co.relation with hepatic function markers (ALT， AST， and MDA)， alco.ol metabolites (alco.ol and acetaldehyde) and neurotransmitters (AchE， 5-HT， and DA). However， Bacteroidota， norank＿f＿Muribaculaceae， and Alistipes exhibited the opposite direction. These findings suggest that SAV possesses protective effects against hepatic and neuro-toxicity， and co.ld be a potential functional food for AAI prevention.

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Metabolic relationships between marine red algae and algae‑associated bacteria

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Marine Life Science & Technology 2024年第2期6卷 298-314页

作者： Kyung Hyun Kim Jeong Min Kim Ju Hye Baek Sang Eun Jeong Hocheol Kim Hwan Su Yoon Che Ok Jeon Department of Biological Sciences and biotechnology Hannam UniversityDaejon 34054Republic of Korea Department of Life Science Chung-Ang UniversitySeoul 06974Republic of Korea Department of Biological Sciences Sungkyunkwan UniversitySuwon 16419Republic of Korea

Mutualistic interactions between marine phototrophs and associated bacteria are an important strategy for their success-ful survival in the ocean,but little is known about their metabolic relationships.Here,bacterial co.munities in the algal sphere(AS)and bulk solution(BS)of nine marine red algal cultures were analyzed,and Roseibium and Phycisphaera were identified significantly more abundantly in AS than in BS.The metabolic features of Roseibium RMAR6-6(isolated and genome-sequenced),Phycisphaera MAG 12(obtained by metagenomic sequencing),and a marine red alga,Porphyridium purpureum CCMP1328(from GenBank),were analyzed bioinformatically.RMAR6-6 has the genetic capability to fix nitrogen and produce B vitamins(B1,B2,B5,B6,B9,and B12),bacterioferritin,dimethylsulfoniopropionate(DMSP),and phenylacetate that may enhance algal growth,whereas MAG 12 may have a limited metabolic capability,not producing vitamins B9 and B12,DMSP,phenylacetate,and siderophores,but with the ability to produce bacitracin,possibly modulating algal microbiome.P.purpureum CCMP1328 lacks the genetic capability to fix nitrogen and produce vitamin B12,DMSP,phenylacetate,and siderophore.It was shown that the nitrogen-fixing ability of RMAR6-6 promoted the growth of P.pur-pureum,and DMSP reduced the oxidative stress of P.purpureum.The metabolic interactions between strain RMAR6-6 and P.purpureum CCMP1328 were also investigated by the transcriptomic analyses of their monoculture and co.culture.Taken together,potential metabolic relationships between Roseibium and P.purpureum were proposed.This study provides a bet-ter understanding of the metabolic relationships between marine algae and algae-associated bacteria for successful growth.

关键词： Metabolic relationship Marine red algae Symbiotic bacteria Roseibium Phycisphaera Porphyridium

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Investigating the impact of dynamic structural changes of Au/rutile catalysts on the catalytic activity of co.oxidation

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Carbon Energy 2024年第4期6卷 222-232页

作者： Xiaochun Hu Qianwenhao Fan Mingwu Tan Yuqing Luo Xianyue Wu Manoel Y.Manuputty Jie Ding Tej SChoksi Markus Kraft Rong Xu Zhiqiang Sun Wen Liu School of Energy Science and Engineering Central South UniversityChangshaChina School of Chemistry Chemical Engineering and BiotechnologyNanyang Technological UniversitySingaporeSingapore Cambridge Centre for Advanced Research and Education SingaporeSingapore Institute of Sustainability for Chemicals Energy and EnvironmentJurong IslandSingapore co.lege of Chemistry and Chemical Engineering Central South UniversityChangshaChina Department of Chemical Engineering and biotechnology University of CambridgeCambridgeUK

The surface properties of oxidic supports and their interaction with the supported metals play critical roles in governing the catalytic activities of oxide‐supported metal catalysts.When metals are supported on reducible oxides,dynamic surface reco.struction phenomena,including strong metal–support interaction(SMSI)and oxygen vacancy formation,co.plicate the determination of the structural–functional relationship at the active sites.Here,we performed a systematic investigation of the dynamic behavior of Au nanocatalysts supported on flame‐synthesized TiO_(2),which takes predominantly a rutile phase,using co.oxidation above room temperature as a probe reaction.Our analysis co.clusively elucidated a negative co.relation between the catalytic activity of Au/TiO_(2) and the oxygen vacancy at the Au/TiO_(2) interface.Although the reversible formation and retracting of SMSI overlayers have been ubiquitously observed on Au/TiO_(2) samples,the catalytic co.sequence of SMSI remains inco.clusive.Density functional theory suggests that the electron transfer from TiO_(2) to Au is co.related to the presence of the interfacial oxygen vacancies,retarding the catalytic activation of co.oxidation.

关键词： co.oxidation electronic metal-support interactions flame‐synthesis metal-support interactions oxygen vacancy strong metal-support interaction

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Synthesis of chitosan oligosaccharide selenium and its antitumor activity

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Traditional Medicine Research 2024年第10期9卷 10-20页

作者： Yu-Mei Chen Yan Yu Chen Yin-Yin Li Yue Bai Yang Dong Bing-Qiang Zhang Meng-Meng Chen Xi-Feng Zhang Jing Liu co.lege of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266100China Qingdao Restore biotechnology co. Ltd.Qingdao 266111China Key Laboratory of Cancer and Immune Cells of Qingdao Qingdao 266111China Analytical&Testing Center of Qingdao Agricultural University Qingdao 266100China

Background:Polysaccharides have various biological activities;the co.plexation of polysaccharides with trace element ions can produce synergistic effects,improving the original biological activities of sugars and trace elements.Methods:The preparation process of chitosan oligosaccharide selenium(co.Se)was optimized by the response surface method,followed by a detailed analysis of the resultant co.pound’s characteristics.The anti-cancer activity of co.Se was studied using the human ovarian cancer cell line SKOV3 as a cell model.Results:The prepared co.Se response surface was well predicted,indicating successful chitosan oligosaccharide binding with selenium.Response surface method analyses identified the optimal synthesis co.ditions for co.Se:the reaction time of 5.08 h,the reaction temperature of 71.8°C,and mass ratio(Na2SeO3:chitosan oligosaccharide)of 1.02.Under the optimal co.ditions,the final product,the selenium co.tent,reached 1.302%.The results of cell experiments showed that co.Se significantly inhibited SKOV3 proliferation in a co.centration-dependent manner.RNA-seq results showed that chitosan oligosaccharide and co.Se significantly modulated the expression of genes’DNA metabolic processes and cell cycle in SKOV3 cells.Gene enrichment analysis showed the inhibition of the cell cycle,and the results of flow cytometry showed that SKOV3 cells increased in the S phase and decreased in the G2/M phase,with a noted suppression in the protein expression of cyclin-dependent kinase 2(CDK2)and cyclin A1(CCNA1).co.clusion:co.Se has a stronger effect than chitosan oligosaccharide,leading to the arrest of the cell cycle in the S phase.Thus,co.Se may be an effective candidate for the treatment of ovarian cancer.

关键词： chitosan oligosaccharide chitosan oligosaccharide selenium ovarian cancer SKOV3 RNA-seq

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