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Development of an Agrobacterium-mediated CRISPR/Cas9 gene editing system in jute(Corchorus capsularis)

The Crop Journal 2024年第4期12卷 1266-1270页

作者： Shaolian Jiang Qin Li Xiangxue Meng Mengxin Huang Jiayu Yao Chuanyu Wang Pingping Fang Aifen Tao Jiantang Xu Jianmin Qi Shuangxia Jin Liwu Zhang Key Laboratory of Ministry of Education for Genetic Breeding and Multiple Utilization of crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Experiment station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Fujian Public Platform for Germplasm Resources of Bast Fiber crops/Fujian Provincial Key Laboratory of Crop Breeding by Design/Key Laboratory of Ministry of Agriculture and Rural Affairs for Biological Breeding of Fujian and Taiwan crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Lab of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after ***,developing an efficient gene editing system for jute remains a *** this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in *** transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)*** CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng *** on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene *** indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy ***1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated *** CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.

关键词： Jute Agrobacterium-mediated transformation Genome editing Hairy root system

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A novel histone methyltransferase gene Cg SDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

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Journal of Integrative Agriculture 2024年第8期23卷 2633-2648页

作者： Jialing Fu Qingjiang Wu Xia Wang Juan Sun Li Liao Li Li Qiang Xu National Key Laboratory for Germplasm Innovation&Utilization of Horticultural crops College of Horticulture&Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China Institute of Horticultural Sciences Jiangxi Academy of Agricultural SciencesNanchang 330200China Plant Breeding and Genetics Section School of Integrative Plant ScienceCornell UniversityNew York 14853USA Robert W.Holley Center for Agriculture and Health USDA-Agricultural Research ServiceCornell UniversityNew York 14853USA

The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human *** is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in ***,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully *** this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo *** of CgSDG40 corresponded to flesh color change and was highly coexpressed with ***,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter *** localization analysis indicated that CgSDG40 localizes to the *** of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild *** addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild *** together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.

关键词： pummelo SET domain protein epigenetic regulation PSY1 lycopene fruit quality fruit color

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Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL

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The Crop Journal 2024年第4期12卷 1168-1175页

作者： Qiang Cao Zhanwang Zhu Dengan Xu Jianhui Wu Xiaowan Xu Yan Dong Yingjie Bian Fugong Ding Dehui Zhao Yang Tu Ling Wu Dejun Han Caixia Lan Xianchun Xia Zhonghu He Yuanfeng Hao College of Agronomy Northwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Food crops Institute Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central ChinaWuhan 430064HubeiChina Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066SichuanChina Hubei Hongshan Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina CIMMYT-China Office C/O CAASBeijing 100081China

Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai *** fine-map the YR86 locus,we developed two large F2 populations from crosses Emai 580/Zhongmai 895 and Avocet S/Zhongmai ***,both populations exhibited suppressed recombination in the same 2AL *** analysis across Chinese Spring,Aikang 58,and 10+wheat genomes revealed a 4.1 Mb chromosomal inversion spanning 708.5-712.6 Mb in the Chinese Spring reference *** markers were developed in the breakpoint and were used to assess a wheat cultivar panel,revealing that Chinese Spring,Zhongmai 895,and Jimai 22 shared a common sequence named InvCS,whereas Aikang 58,Yangmai 16,Emai 580,and Avocet S shared the sequence named *** inverted configuration explained the suppressed recombination observed in all three bi-parental *** recombination was observed in a Jimai 22/Zhongmai 895 F2 population,facilitating mapping of YR86 to a genetic interval of 0.15 cM corresponding to 710.27-712.56 Mb falling within the inverted ***-three high-confidence genes were annotated in the interval using the Chinese Spring reference genome,with six identified as potential candidates for YR86 based on genome and transcriptome *** results will accelerate map-based cloning of YR86 and its deployment in wheat breeding.

关键词： Adult-plant resistance Chromosomal inversion Puccinia striiformis Triticum aestivum

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Retention of harvest residues promotes the accumulation of topsoil organic carbon by increasing particulate organic carbon in a Chinese fir plantation

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Forest Ecosystems 2024年第5期11卷 720-727页

作者： Jiamin Yang Ke Huang Xin Guan Weidong Zhang Renshan Li Longchi Chen Silong Wang Qingpeng Yang Huitong Experimental station of Forest Ecology CAS Key Laboratory of Forest Ecology and ManagementInstitute of Applied EcologyShenyang110016China Huitong National Research station of Forest Ecosystem Hunan Key Laboratory for Structure and Ecosystem Service of Subtropical ForestHuaihua418307China Life Science Department Luoyang Normal UniversityLuoyang471934China College of Resources and Environment University of Chinese Academy of SciencesBeijing101408China

Background As commonly used harvest residue management practices in subtropical plantations,stem only harvesting(SOH)and whole tree harvesting(WTH)are expected to affect soil organic carbon(SOC)***,knowledge on how SOC and its fractions(POC:particulate organic carbon;MAOC:mineral-associated organic carbon)respond to different harvest residue managements is *** In this study,a randomized block experiment containing SOH and WTH was conducted in a Chinese fir(Cunninghamia lanceolata)*** effect of harvest residue management on SOC and its fractions in topsoil(0–10cm)and subsoil(20–40cm)was *** inputs(harvest residue retaining mass and fine root biomass)and microbial and mineral properties were also *** The responses of SOC and its fractions to different harvest residue managements varied with soil ***,SOH enhanced the content of SOC and POC in topsoil with increases of 15.9%and 29.8%,respectively,compared with ***,SOH had no significant effects on MAOC in topsoil and SOC and its fractions in *** results indicated that the increase in POC induced by the retention of harvest residue was the primary contributor to SOC accumulation,especially in *** harvest residue managements affected SOC and its fractions through different pathways in topsoil and *** plant inputs(the increase in fine root biomass induced by SOH)exerted a principal role in the SOC accumulation in topsoil,whereas mineral and microbial properties played a more important role in regulating SOC dynamics than plants inputs in *** The retention of harvest residues can promote SOC accumulation by increasing POC,and is thus suggested as an effective technology to enhance the soil carbon sink for mitigating climate change in plantation management.

关键词： Chinese fir plantation Soil organic carbon Particulate organic carbon Mineral-associated organic carbon Harvest residue management

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Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae

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Horticultural Plant Journal 2024年第4期10卷 1049-1060页

作者： Wenlin Yu Lu Yang Yuanyuan Xiang Rongde Li Xueqing Zhou Longcai Gan Xianyu Xiang Yunyun Zhang Lei Yuan Yanqing Luo Genze Li Youning Wang Yinhua Chen Peng Chen Chunyu Zhang National Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China National Key Laboratory for Tropical Crop Breeding Sanya Nanfan Research InstituteHainan UniversitySanyaHainan 572025China National Agro-Tech Extension and service Center Beijing 100125China Industrial crops Research Institute Yunnan Academy of Agricultural SciencesKunmingYunnan 650221China College ofAgronomy NorthwestA&F UniversityYanglingShaanxi712100China

Many economically important crops and vegetables belonging to the cruciferous family are heavily endangered by clubroot disease caused by Plasmodiophora brassicae *** of clubroot resistant cultivars based on mapping and cloning of resistant genes is commonly regarded as the most cost-effective and efficient way to fight against this *** traditional way of R gene functional validation requires stable transformation that is both time-and *** this study,a rapid and efficient hairy-root transgenic protocol mediated by Agrobacterium rhizogenes was *** transformation positive rate was over 80%in Brassica napus showed by GUS reporter gene and this transformation only took 1/6 of the time compared with stable *** system was applicable to different *** varieties and other cruciferous crops including Brassica rapa and Brassica *** particular,two known CR genes,CRA3.7.1 and CRA8.2.4 were used respectively,as example to show that the system works well for CR gene study combined with subsequent *** infection in *** importantly,it works both in over-expression that led to disease resistance,as well as in RNAi which led to disease susceptible ***,this system can be used in batch-wise identification of CR genes,and also offered the possibility of manipulating key genes within the *** genome that could improve our knowledge on host-pathogen interaction.

关键词： Brassicaceae Agrobacterium rhizogenes Hairy root transformation Clubroot Gene function

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Assessing the effect of invasive organisms on forests under information uncertainty: The case of pine wood nematode in continental Europe

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Forest Ecosystems 2024年第5期11卷 685-696页

作者： Nick Schafstall Laura Dobor Marco Baldo Andrew MLiebhold Werner Rammer Juha Honkaniemi Tomás Hlásny Faculty of Forestry and Wood Sciences Czech University of Life SciencesPragueCzech Republic US Forest service Northern Forest Research station MorgantownUSA Ecosystem Dynamics and Forest Management Group Technical University of MunichGermany Natural Resources Institute Finland HelsinkiFinland

Forests worldwide are experiencing increasingly intense biotic disturbances;however,assessing impacts of these disturbances is challenging due to the diverse range of organisms involved and the complex interactions among *** particularly applies to invasive species,which can greatly alter ecological processes in their invaded *** we focus on the pine wood nematode(PWN,Bursaphelenchus xylophilus),an invasive pathogen that has caused extensive mortality of pines in East Asia and more recently has invaded southern *** is expected to expand its range into continental Europe with heavy impacts *** the unknown dynamics of PWN in continental Europe,we reviewed laboratory and field experiments conducted in Asia and southern Europe to parameterize the main components of PWN biology and host-pathogen interactions in the Biotic Disturbance Engine(BITE),a model designed to implement a variety of forest biotic agents,from fungi to large *** simulate dynamically changing host availability and conditions,BITE was coupled with the forest landscape model *** potential impacts of introducing PWN were assessed in a Central European forest landscape(40,928ha),likely within PWN’s reach in future decades.A parameter sensitivity analysis indicated a substantial influence of factors related to dispersal,colonization,and vegetation impact,whereas parameters related to population growth manifested a minor *** of different assumptions about biological processes resulted in differential timing and size of the main mortality wave,eliminating 40%–95%of pine trees within 100 years post-introduction,with a maximum annual carbon loss between 1.3%and 4.2%.PWN-induced tree mortality reduced the Gross Primary Productivity,increased heterotrophic respiration,and generated a distinct legacy sink effect in the recovery *** assessment has corroborated the ecological plausibility of the simulated dynamics and highlighted the need for

关键词： Biological invasions Process-based modelling Forest ecosystems Model parameters

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Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response

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Virologica Sinica 2024年第3期39卷 501-512页

作者： Wen-Jun Tian Xiu-Zhong Zhang Jing Wang Jian-Feng Liu Fu-Huang Li Xiao-Jia Wang National Key Laboratory of Veterinary Public Health and Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology China Agricultural UniversityBeijing 100193China Beijing General station of Animal Husbandry service(South Section) Beijing102218China

The infection caused by porcine epidemic diarrhea virus(PEDV)is associated with high mortality in piglets *** factors involved in the efficient replication of PEDV,however,remain largely *** recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5(EF-hand protein calmodulin-like 5)following PEDV infection.A further study unveiled a biphasic increase of CALML5 in 2 and 12 h after viral *** trends were observed in the intestines of piglets in the early and late stages of the PEDV ***,CALML5 depletion reduced PEDV mRNA and protein levels,leading to a one-order-of-magnitude decrease in virus *** the early stage of PEDV infection,CALML5 affected the endosomal trafficking pathway by regulating the expression of endosomal sorting complex related cellular ***5 depletion also suppressed IFN-βand IL-6 production in the PEDV-infected cells,thereby indicating its involvement in negatively regulating the innate immune *** study reveals the biological function of CALML5 in the virology field and offers new insights into the PEDV-host cell interaction.

关键词： Porcine epidemic diarrhea virus(PEDV) EF-hand protein calmodulin-like 5(CALML5) Late endosomes Innate immune response

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Monitoring absolute vertical land motions and absolute sea-level changes from GPS and tide gauges data over French Polynesia

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Geodesy and Geodynamics 2024年第1期15卷 13-26页

作者： Xianjie Li Jean-Pierre Barriot Bernard Ducarme Marania Hopuare Yidong Lou Geodesy Observatory of Tahiti University of French PolynesiaFaa'a 98702French Polynesia Catholic University of Louvain Georges Lemaître Centre for Earth and Climate Research(ELI)1348 Louvain-la-NeuveBelgium GNSS Research Center Wuhan UniversityWuhan 430079China State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhan 430079China

In this study,we estimate the absolute vertical land motions at three tidal stations with collocated Global Navigation Satellite System(GNSS)receivers over French Polynesia during the period 2007-2020,and obtain,as ancillary results,estimates of the absolute changes in sea level at the same *** verify our processing approach to determining vertical motion,we first modeled vertical motion at the International GNSS service(IGS)THTI station located in the capital island of Tahiti and compared our estimate with previous independent determinations,with a good *** obtained the following estimates for the vertical land motions at the tide gauges:Tubuai island,Austral Archipelago-0.92±0.17 mm/yr,Vairao village,Tahiti Iti:-0.49±0.39 mm/yr,Rikitea,Gambier Archipelago-0.43±0.17 mm/*** absolute variations of the sea level are:Tubuai island,Austral Archipelago 5.25±0.60 mm/yr,Vairao village,Tahiti Iti:3.62±0.52 mm/yr,Rikitea,Gambier Archipelago 1.52±0.23 mm/*** discuss these absolute values in light of the values obtained from altimetric measurements and other means in French Polynesia.

关键词： GPS Tide gauges Sea level changes Vertical land motion

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Assessment of coastal and mangrove vulnerability in the Andaman Island,Indian Ocean

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Geoscience Frontiers 2024年第5期15卷 47-60页

作者： Biswajit Mondal Rupesh K.Bhomia Ashis Kumar Saha Richard A.MacKenzie Department of Geography Delhi School of EconomicsUniversity of DelhiDelhi 110007India Centre for International Forestry Research(CIFOR) Jalan CIFORSitu GedeBogor BaratBogor 16115Indonesia Institute of Pacific Islands Forestry Pacific Southwest Research StationUSDA Forest ServiceHiloHI 96720USA

Coastal regions are highly susceptible to the effects of global warming,including rising atmospheric and sea surface temperatures,increased cyclone frequency,and sea level ***,it is imperative to examine coastal vulnerability to minimize the impact of multiple hazards and protect coastal resources,such as *** in India studying the vulnerability of coastal zones of Andaman and Nicobar Islands which fall in seismic zone V is critical for conservation *** conducted a vulnerability analysis of coastal zones impacted by the 2004 earthquake,causing varying degrees of ground upliftment and *** compared coastal vulnerability among sites that experienced uplift,no change,and subsidence(the southern portion).Our analysis utilized the Coastal and Mangrove Vulnerability Index(CVI and MVI)to measure and compare vulnerability in six zones distributed along uplift and subsidence ***-resolution satellite imagery including WorldView-2,3,and GeoEye-1 from year 2022 are utilized on this *** CVI and MVI offers a good way to measure and compare vulnerabilities across sites and offer insights for better *** CVI and MVI results indicate that approximately 34%of coastal grids and over 23%of mangrove grids across all zones are highly to extremely highly *** zones were found to be more vulnerable than uplifted *** findings suggest that large-scale natural disturbances such as tectonic displacement have the potential to impact coastal vegetation and mangrove cover can become even more *** conclusion,our study emphasizes the importance of vulnerability analyses in coastal regions,especially in areas prone to seismic *** findings have direct implications for conservation and restoration efforts and underscore the need for continued monitoring and mitigation efforts to safeguard coastal resources for long-term sustainability.

关键词： Mangroves Coastal vulnerability Mangrove vulnerability 2004 Indian Ocean earthquake Andaman Island

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Combined application of organic fertilizer and chemical fertilizer alleviates the kernel position effect in summer maize by promoting post-silking nitrogen uptake and dry matter accumulation

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Journal of Integrative Agriculture 2024年第4期23卷 1179-1194页

作者： Lichao Zhai Lihua Zhang Yongzeng Cui Lifang Zhai Mengjing Zheng Yanrong Yao Jingting Zhang Wanbin Hou Liyong Wu Xiuling Jia Institute of Cereal and Oil crops Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Crop Cultivation Physiology and Green Production of Hebei ProvinceShijiazhuang 050035China Hebei Science and Technology Innovation service Center Shijiazhuang 050051China Yongnian Agricultural Technology Extension Center Handan 057150China Ningjin Agricultural Technology Extension Center Xingtai 055550China

Adjusting agronomic measures to alleviate the kernel position effect in maize is important for ensuring high *** order to clarify whether the combined application of organic fertilizer and chemical fertilizer(CAOFCF)can alleviate the kernel position effect of summer maize,field experiments were conducted during the 2019 and 2020 growing seasons,and five treatments were assessed:CF,100%chemical fertilizer;OFCF1,15%organic fertilizer+85%chemical fertilizer;OFCF2,30%organic fertilizer+70%chemical fertilizer;OFCF3,45%organic fertilizer+55%chemical fertilizer;and OFCF4,60%organic fertilizer+40%chemical *** with the CF treatment,the OFCF1 and OFCF2 treatments significantly alleviated the kernel position effect by increasing the weight ratio of inferior kernels to superior kernels and reducing the weight gap between the superior and inferior *** effects were largely due to the improved filling and starch accumulation of inferior ***,there were no obvious differences in the kernel position effect among plants treated with CF,OFCF3,or OFCF4 in most *** area indexes,post-silking photosynthetic rates,and net assimilation rates were higher in plants treated with OFCF1 or OFCF2 than in those treated with CF,reflecting an enhanced photosynthetic capacity and improved postsilking dry matter accumulation(DMA)in the plants treated with OFCF1 or *** with the CF treatment,the OFCF1 and OFCF2 treatments increased post-silking N uptake by 66.3 and 75.5%,respectively,which was the major factor driving post-silking photosynthetic capacity and ***,the increases in root DMA and zeatin riboside content observed following the OFCF1 and OFCF2 treatments resulted in reduced root senescence,which is associated with an increased post-silking N *** showed that post-silking N uptake,DMA,and grain yield in summer maize were negatively correlated with the kernel position *** conclusion,the combined application of

关键词： chemical fertilizer dry mater accumulation kernel position effect N uptake organic fertilizer

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