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Optimizing hairy root production from explants of Phyllanthus hainanensis,a shrub used for traditional herbal medicine

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Frontiers of Agricultural Science and engineering 2020年第4期7卷 513-522页

作者： Zhaogui YAN Shengyu LIU Junlian ZHANG Guan HUANG Lijun DUAN Yaomei YE College of Horticulture and Forestry Sciences/Hubei engineering Technology Research Center for Forestry Information Huazhong Agricultural UniversityWuhan 430070China School of Environmental&Rural Science The University of New EnglandArmidaleNSW 2351Australia wuhan garden constructional engineering company Wuhan 430050China Hubei Wildlife and Plant Conservation Agency Wuhan 430070China

Phyllanthus hainanensis is a shrub that has been used in traditional herbal medicine.It has great pharmaceutical potential for treating diseases such as cancer and diabetes.As a prerequisite for propagation of this species on a large scale,hairy roots in P.hainanensis were induced using Rhizobium rhizogenes and various factors affecting hairy root induction and growth evaluated.Seven factors were tested:(1)type of explant,(2)type of culture medium,(3)duration of pre-culture,(4)R.rhizogenes inoculum cell density,(5)duration of infection,(6)acetosyringone concentration in the culture medium,and(7)duration of incubation.The optimal protocol for hairy root induction and growth was:young shoots,pre-cultured in Y1 for 2 d,inoculated with R.rhizogenes broth with an OD600 of 0.6 for 20 min,and incubated for 3 d.Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction.Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of P.hainanensis.Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.

关键词： hairy root induction medicinal plant MS growth medium Phyllanthus hainanensis Rhizobium rhizogenes

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Cumulative damage effects of mainshock-aftershock sequences on RC-frame structures

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Earthquake Science 2019年第3期32卷 157-169页

作者： Lingxin Zhang Baijie Zhu Yunqin Xue Jialu Ma Institute of engineering Mechanics China Earthquake AdministrationHarbin 150080China Key Laboratory of Earthquake engineering and engineering Vibration China Earthquake AdministrationHarbin 150080China Country garden Holdings company Limited Xinxiang 453000 China

Frequent aftershocks often follow a strong mainshock.They can significantly increase cumulative damage to a structure.A model of a five-story reinforced concrete frame structure was designed and a nonlinear mathematical model of the structure was developed to investigate the damage states resulting from different mainshock-aftershock sequences.Mainshock-aftershock sequences consisting of one of three recorded mainshocks combined with one of five recorded aftershocks were created for input to the mathematical model.Inelastic energy dissipation and the Park-Ang damage index were used as measures of cumulative damage to the structure.The results demonstrate that consideration of only the single mainshock ground motion in seismic building design can result in the design and construction of unsafe buildings.Total cumulative damage to a structure is caused by the combination of damage states resulting from the mainshock and the aftershock(s).

关键词： mainshock-aftershock RC frame cumulative damage Park-Ang damage index inelastic energy dissipation

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High Strength Low Alloy (HSLA) Steels in Building Construction

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Journal of Iron and Steel Research(International) 2011年第S1期18卷 773-778页

作者： Sivakumaran Ken Siva Jansto Steven G Department of Civil engineering McMaster UniversityHamilton Reference Metals company-CBMM 1000 Old Pond RoadBridgevillePA 15017USA

Majority of the buildings,including industrial buildings,are constructed using either structural steel (plates and structural shapes) or deformed bar steel reinforced concrete.Such buildings,however,must be designed to be safe and serviceable during construction and during use and occupancy.These objectives can be easily achieved by the use of steels having superior mechanical properties,ductility,weldability,fire resistance,etc.Over the years,the steel industry has made improvements in steel making technologies resulting in high strength low alloy (HSLA) steels with superior steel properties well suited for building construction.First part of this paper presents the structural design considerations,and the constructional considerations associated with the building structures in general,and steel structures in particular.This second part of the paper looks at the acceptance criteria for HSLA steels for North American building codes and construction.The third part of the paper presents the structural properties of currently available HSLA steels for building construction.The discussion focuses on hot-rolled structural steel shapes as well as deformed steel bars for concrete reinforcement.The paper argues that Niobium microalloying is the key to achieving superior properties in such steels.

关键词： HSLA steels building construction structural shapes deformed bars structural performance niobium

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A Study on the Interest Linkage Mechanism of the Taxus Industry Chain in Pengshui Miao and Tujia Autonomous County

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Agricultural Biotechnology 2023年第5期12卷 22-25页

作者： Zhengchun WANG Guifang CHEN Haiyan JIANG Guohao ZHANG Yan TIAN Jing XIN Jihui LI Chongqing Institute of Forestry Sciences Chongqing 400000China Guangzhou Hanye garden engineering Co. Ltd.Chongqing 400000China Forestry Science and Technology Promotion Station of Pengshui Miao and Tujia Autonomous County Chongqing 400000China

At the Taxus base of Pengshui Miao and Tujia Autonomous County,Chongqing,the comprehensive development and utilization of Taxus was carried out with seedling cultivation,understory economy,product processing,ecotourism and other aspects as the main line.The benefit linkage mechanism of the operation of the Taxus industrial chain was explored and analyzed,so as to extend and supplement the Taxus industrial chain.The two models of"company+farmer"and"company+village collective+farmer"are relatively close benefit linkage models,which indirectly promote the increase of farmers income and smoothly guarantee the operation of the industrial chain,thereby playing a positive role during the development of the Taxus industry.This study provides reliable countermeasures and suggestions for the sustained and healthy development of the Taxus industry in Pengshui County.

关键词： Taxus Development and utilization Industrial chain Interest linkage Mechanism

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Effects of dry fog humidification on pericarp browning and quality of litchi fruit stored at low temperature

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International Journal of Agricultural and Biological engineering 2019年第4期12卷 192-196页

作者： Lu Xiao Taotao Li Guoxiang Jiang Afiya John Dandan Zhang Wenyuan Jin Xuewu Duan Yueming Jiang Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Post-Harvest Handling of Fruits Ministry of Agriculture and Rural Affairs/Key Laboratory of Plant Resources Conservation and Sustainable UtilizationSouth China Botanical GardenChinese Academy of SciencesGuangzhou 510650China University of Chinese Academy of Sciences Beijing 100049China Suzhou Full Fortune Food company Suzhou 215111China

Pericarp browning is the major cause of deterioration of harvested litchi fruit.Water loss plays a role in pericarp browning of litchi fruit.This study investigated the effects of humidification with dry fog on pericarp browning and quality of litchi fruit stored at low temperature.Litchi fruit were stored in a non-humidified cold chamber(control)or in a humidified cold room using Tabor atomizer system that generated 95%relative humidity(RH)without depositing water on the fruit surface at 4℃.Control fruit stored in cold room without added humidity underwent rapid weight loss,accompanied by severe pericarp browning after 25 d of storage.However,slight weight loss and no obvious pericarp browning were found in humidified-fruit.Moreover,humidification maintained well the integrity of cell membrane and inhibited polyphenol oxidase activity during early storage.In addition,respiration rate was obviously inhibited in humidified-fruit compared with control fruit.This study might provide a convenient approach to reduce pericarp browning of harvested litchi fruit by humidifying the fruit using the Tabor atomizer at low temperature instead of packaging with film.

关键词： litchi pericarp browning dry fog high relative humidity cold storage

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Impact of land use and land cover changes on carbon storage in rubber dominated tropical Xishuangbanna,South West China

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Ecosystem Health and Sustainability 2021年第1期7卷 121-134页

作者： Chaya Sarathchandra Yirga Alemu Abebe Fiona Ruth Worthy Iresha Lakmali Wijerathne Huaixia Ma Bi Yingfeng Guo Jiayu Huafang Chen Qiaoshun Yan Yanfei Geng Dayani SWeragoda Li-Li Li Yang Fengchun Sriyani Wickramasinghe Jianchu Xu State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina University of Chinese Academy of Sciences Beijing 100049China Department of Biological Science Faculty of Applied SciencesRajarata University of Sri LankaMihintale Sri Lanka State Key Laboratory of Super Lattices and Microstructures Institute of SemiconductorsChinese Academy of SciencesBeijing China Department of Electrical and computer engineering Faculty of Electrical EngineeringInstitute of TechnologyHawassa UniversityHawassaEthiopia World Agroforestry Centre(ICRAF) East and Central Asia Regional OfficeKunming YunnanChina Ailaoshan Station for Subtropical Forest Ecosystem Studies Chinese Academy of SciencesJingdongYunnan College of Tea Science Guizhou UniversityGuiyang China Carbon Consulting company Colombo Sri Lanka Center for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglaYunnanChina Yibin Vocational and Technical College YibinSichuanChina School of Chinese Medicinal Material Resources Guangdong Pharmaceutical UniversityChina

Land use and land cover(LULC)play a significant role in carbon regulation.South-China accounts for~65%of China’s carbon sink.In Xishuangbanna(South-China),rubber is expanding rapidly creating an urgent need to understand and monitor LULC change and how spatial variation affects carbon storage(CS).This is vital for the formation and implementation of better land use management practices.We studied LULC changes of 22-year period;addressing how these changes have affected the CS.We quantified LULC changes between1988 and 2010 using remote sensing methods and calculated CS changes using InvEST.Results showed that between 1988 and 2010,the rate of deforestation accelerated to 203.2 km^(2)y^(−1)and~23%of forest were lost.Conversion of natural forest to rubber was responsible for 78%of this deforestation.Rubber expansion rate was 153.4 km^(2)y^(−1).Changes to LULC drove a temporal CS reduction 0.223 Tg C/km^(2).Local stakeholders have strong economic interest in converting land to more profitable plantations.Government efforts is required to control land conversion through new policies and incentives to retain natural forest.Assessment of specific potential land use change will be required to avoid promoting the conversion of high carbon storage land uses to low carbon storage land uses.

关键词： Carbon storage deforestation economic plantations ecosystem services land cover changes land use

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Ultrahigh pressure sintering of polycrystalline diamond cubes

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金刚石与磨料磨具工程 2008年第S1期28卷 70-76,79页

作者： Hu Shao-Chung Chang Kuen-Liang Sung James C. Tso Pei-Lum Hsu Kai-Hung Sung Michael KINIK company 64Chung-San Rd.Ying-Kuo National Taipei University of Technology National Taiwan University PME Dept.National Tsing Hua University Advanced Diamond Solutions Inc.351 King Street Suite 813San FranciscoCA 94158U.S.A.

Polycrystalline grits(polygrits) have been used widely in industry as abrasives.Due to their higher impact strength and the friable nature,polygrits are more endurable and more efficient than monocrystal grits (monogrits) in material removal applications.For example,polygrits of alumina may last longer and cut faster than monogrits.Similarly,polygrits of cubic boron nitride is a superior superabrasive than monogrits of the same. Although diamond superabrasives have been indispensable for constructional sawing applications and industrial grinding applications,there has no polygrits of diamond that are commercially available.In this research,we used a novel technology to sinter micron diamond fines to form polygrits of diamond.The characterization demonstrated that polygrits of diamond can be tough to withstand impact.But due to the presence of micro grains,the grit can sharpen itself without dulling by shedding the worn grains.This paper revealed many examples of using diamond polygrits.

关键词： PCD superabrasives diamond grits CMP pad conditioners

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