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Synthetic chromosome evolves the yeast genome

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Science china(Life Sciences) 2019年第5期62卷 708-709页

作者： Fei Teng Wei Li Qi Zhou State Key Laboratory of stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences institute for stem cell and Regeneration Chinese Academy of Sciences University of chinese Academy of Sciences

Genomic structural variations play a critical role in biological function and phenotypic diversity. Although smallscale variations, such as insertion, deletion, and point mutations, have been extensively investigated, studies on largescale structural variations such as segmental duplication,inversion, and chromosome-scale variations remain *** the recent decade。

关键词： Li Synthetic chromosome evolves the yeast genome Figure

Editing base in mouse model

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Protein & cell 2017年第8期8卷 558-559页

作者： Haoyi Wang State Key Laboratory of stem cell and Reproductive Biology Institute of Zoology Chinese Academy of SciencesBeijing 100101 China

Genome editing technologies enable scientists to modify DNA sequence at specific genomic loci in various cells and species. There are several editing tools, including homing endonucleases （Silva et al., 2011）, zinc finger nucleases （ZFN）（Urnov et al., 2010）, transcription activator-like effector nucleases （TALENs）（Bogdanove and Voytas, 2011）, and clustered regularly interspaced short palindromic repeats （cRISPR） and the cRISPR-associated proteins （cas） system （Doudna and charDentier, 2014）.

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The day after the 7th day of the creation:Breakthrough of human embryo in vitro culture

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Science china(Life Sciences) 2017年第6期60卷 665-667页

作者： Hui Gao Wei Li State Key Laboratory of stem cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Since the creation of intelligent creatures,we are no longer the puppets of the *** thousands of years,people have been thinking about the nature and the future of ourselves,and reconstruction of the process of life has become the ultimate dream for *** 1951,William Harvey,a British scientist,elaborated the theory of the structure and function of embryos for the first time in his book'the reproduction of animals',which established the foundation for people to explore the process and the mechanism of embryonic development.

关键词： embryo ultimate thinking Harvey intelligent creation creation reproduction oocyte dream

Identification and characterization of human hematopoietic mesoderm

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Science china(Life Sciences) 2024年第2期67卷 320-331页

作者： Yuqi Wen Jingjing Zhao Runqing Zhang Fan Liu Xiaoyuan chen Dan Wu Mengge Wang cuicui Liu Pei Su Panpan Meng Yiyue Zhang Xin Gao Lu Wang Hongtao Wang Jiaxi Zhou State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Tianjin institutes of Health Science Tianjin 301600China cAMS center for stem cell Medicine PUMC Department of Stem Cell and Regenerative MedicineTianjin 300020China Division of cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhou 510006China

The embryonic mesoderm comprises heterogeneous cell subpopulations with distinct lineage *** is unclear whether a bias for the human hematopoietic lineage emerges at this early developmental *** this study,we integrated single-cell transcriptomic analyses of human mesoderm cells from embryonic stem cells and embryos,enabling us to identify and define the molecular features of human hematopoietic mesoderm(HM)cells biased towards hematopoietic *** discovered that BMP4 plays an essential role in HM specification and can serve as a marker for HM ***,BMP4 acts as a downstream target of HDAc1,which modulates the expression of BMP4 by deacetylating its *** of HDAc significantly enhances HM specification and promotes subsequent hematopoietic cell *** conclusion,our study identifies human HM cells and describes new mechanisms for human hematopoietic development.

关键词： hematopoietic mesoderm human pluripotent stem cells human embryos BMP4 HDAc1

Application of metabolomics in urolithiasis:the discovery and usage of succinate

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Signal Transduction and Targeted Therapy 2023年第2期8卷 771-783页

作者： Xiu-zhen Zhang Xiong-xin Lei Yan-lin Jiang Long-mei Zhao chen-yu Zou Yun-jin Bai Ya-xing Li Rui Wang Qian-jin Li Qiu-zhu chen Ming-hui Fan Yu-ting Song Wen-qian Zhang Yi Zhang Jesse Li-Ling Hui-qi Xie Laboratory of stem cell and Tissue Engineering Orthopedic Research InstituteDepartment of OrthopedicsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Urology Institute of UrologyWest China HospitalSichuan UniversityChengduSichuan 610041China Research core Facility of west china Hospital Sichuan UniversityChengduSichuan 610041China Department of Medical Genetics West China Second University HospitalChengduSichuan 610041China

Urinary stone is conceptualized as a chronic metabolic disorder punctuated by symptomatic stone *** has been shown that the occurrence of calcium oxalate monohydrate(cOM)during stone formation is regulated by crystal growth *** crystallization inhibitors have been recognized as a therapeutic modality for decades,limited progress has been made in the discovery of effective modifiers to intervene with stone *** this study,we have used metabolomics technologies,a powerful approach to identify biomarkers by screening the urine components of the dynamic progression in a bladder stone *** in-depth mining and analysis of metabolomics data,we have screened five differential *** density functional theory studies and bulk crystallization,we found that three of them(salicyluric,gentisic acid and succinate)could effectively inhibit nucleation in *** thereby assessed the impact of the inhibitors with an EG-induced rat model for kidney ***,succinate,a key player in the tricarboxylic acid cycle,could decrease kidney calcium deposition and injury in the *** analysis further showed that the protective effect of succinate was mainly through anti-inflammation,inhibition of cell adhesion and osteogenic *** findings indicated that succinate may provide a new therapeutic option for urinary stones.

关键词： crystallization thereby protective

Progress in clinical trials of stem cell therapy for cerebral palsy

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Neural Regeneration Research 2021年第7期16卷 1377-1382页

作者： Zhong-Yue Lv Ying Li Jing Liu stem cell clinical Research center National Joint Engineering LaboratoryRegenerative Medicine CenterThe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation institute of stem cell and Precision Medicine DalianLiaoning ProvinceChina

cerebral palsy is the most common disease in children associated with lifelong disability in many *** research has demonstrated that traditional physiotherapy and rehabilitation therapies cannot alone cure cerebral *** cell transplantation is an emerging therapy that has been applied in clinical trials for a variety of neurological diseases because of the regenerative and unlimited proliferative capacity of stem *** this review, we summarize the design schemes and results of these clinical *** findings reveal great differences in population characteristics, stem cell types and doses, administration methods, and evaluation methods among the included clinical ***, we also assess the safety and efficacy of these clinical *** anticipate that our findings will advance the rational development of clinical trials of stem cell therapy for cerebral palsy and contribute to the clinical application of stem cells.

关键词： adverse events brain cell transplantation central nervous system cerebral palsy clinical trials plasticity regeneration stem cell

A neurovascular unit-on-a-chip:culture and differentiation of human neural stem cells in a three-dimensional microfluidic environment

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Neural Regeneration Research 2022年第10期17卷 2260-2266页

作者： Wen-Juan Wei Ya-chen Wang Xin Guan Wei-Gong chen Jing Liu stem cell clinical Research center National Joint Engineering LaboratoryRegenerative Medicine Centerthe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation institute of stem cell and Precision Medicine DalianLiaoning ProvinceChina

Biological studies typically rely on a simple monolayer cell culture,which does not reflect the complex functional characteristics of human tissues and organs,or their real response to external *** technology has advantages of high-throughput screening,accurate control of the fluid velocity,low cell consumption,long-term culture,and high *** combining the multipotential differentiation of neural stem cells with high throughput and the integrated characteristics of microfluidic technology,an in vitro model of a functionalized neurovascular unit was established using human neural stem cell-derived neurons,astrocytes,oligodendrocytes,and a functional microvascular *** model comprises a multi-layer vertical neural module and vascular module,both of which were connected with a syringe *** provides controllable conditions for cell inoculation and nutrient supply,and simultaneously simulates the process of ischemic/hypoxic injury and the process of inflammatory factors in the circulatory system passing through the blood-brain barrier and then acting on the nerve tissue in the *** in vitro functionalized neurovascular unit model will be conducive to central nervous system disease research,drug screening,and new drug development.

关键词： (neural)differentiation astrocyte blood-brain barrier brain microvascular endothelial cells central nervous system microfluidics neural stem cells neuron neurovascular unit oligodendrocyte organ-on-a-chip

Mesenteric lymph system constitutes the second route in gut-liver axis and transports metabolism-modulating gut microbial metabolites

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Journal of Genetics and Genomics 2022年第7期49卷 612-623页

作者： Ying Yu Bin Liu Xiaolin Liu Xuan Zhang Wenhui Zhang He Tian Guanghou Shui Wenzhao Wang Moshi Song Jun Wang cAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of chinese Academy of Sciences Beijing 100049China college of Pulmonary and critical care Medicine Chinese PLA General HospitalBeijing 100853China Medical School of chinese PLA Beijing 100853China State Key Laboratory of Molecular developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China institute for stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing institute for stem cell and Regenerative Medicine Beijing 100101China

The gut-liver axis denotes the intricate connection and interaction between gut microbiome and liver, in which compositional and functional shifts in gut microbiome affect host metabolism. Hepatic portal vein of the blood circulation system has been thought to be the major route for metabolite transportation in the gut-liver axis, but the existence and importance of other routes remain elusive. Here, we perform metabolome comparison in blood circulation and mesenteric lymph systems and identify significantly shifted metabolites in serum and mesentery. Using cellular assays, we find that the majority of decreased metabolites in lymph system under high-fat diet are effective in alleviating metabolic disorders, indicating a high potential of lymph system in regulating liver metabolism. Among those, a representative metabolite, L-carnitine, reduces diet-induced obesity in mice. Metabolic tracing analysis identifies that L-carnitine is independently transported by the mesenteric lymph system, serving as an example that lymph circulation comprises a second route in the gut-liver axis to modulate liver metabolism. Our study provides new insights into metabolite transportation via mesenteric lymph system in the gut-liver axis, offers an extended scope for the investigations in host-gut microbiota metabolic interactions and potentially new targets in the treatment of metabolic disorders.

关键词： Mesenteric lymph system Gut-liver axis Gut microbial metabolites Host metabolism

Single-cell sequencing delivers hematopoietic stem cell specification

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Science Bulletin 2016年第18期61卷 1419-1421页

作者： Jiani cao Tongbiao Zhao State Key Laboratory of stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences

Recently,Zhou et al.[1]have successfully traced the hematopoietic stem cell(HSc)specification during the stepwise development of HSc from the pre-HSc stage at single-cell *** findings offer fundamental insights into mouse HSc fate decision and provide extensive information that may lead to novel discoveries in HSc ***,standing at the apex of the hierarchy

关键词：造血干细胞单细胞测序 HSc 细胞分化分辨率扩展性小鼠