检索结果-南通市图书馆

Multiple Signaling Pathways Control Tbx6 Expression during Xenopus Myogenesis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第6期36卷 390-396页

作者： Pan-Feng FANG, Rui-Ying HU, Xing-Yue HE, and Xiao-Yan DING* Laboratory of molecular and cell biology, Laboratory of Stem cell biology & institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China Laboratory of molecular and cell biology Laboratory of Stem Cell Biology & Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

Tbx6 is critical for somite specification and myogenesis initiation. It has been shown that Activin/Nodal, VegT/Nodal, FGF, and BMP signaling pathways are involved early in specifying mesoderm or later in patterning mesoderm, and Xnot plays roles in setting up the boundary between notochord and paraxial mesoderm. In this study, we introduce the dominant negative form of above genes into embryos to evaluate if they are responsible for regulating Tbx6 expression. The results show that: (1) Activin/Nodal and VegT/Nodal signals are necessary for both initiation and maintenance of Tbx6 expression, and Nodal is sufficient to induce ectopic Tbx6 expression; (2) FGF signal is necessary for the initiation and maintenance of Tbx6, but it is not sufficient to induce Tbx6 expression; (3) BMP is also necessary for the expression of Tbx6, and the induction of Tbx6 expression by BMP is dose dependent; (4) Xnot has no effect on the expression of Tbx6. Our results suggest that several signaling pathways are involved in regulating Tbx6 expression, and pave the route to reveal the molecular mechanism of initiating myogenesis.

关键词： Tbx6 FGF BMP Activin/Nodal VegT/Nodal

Recent advances in exploring new blood-based biomarkers for the early diagnosis of gastric cancer

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2025年第1期68卷 35-45页

作者： Xinyu Peng Qian Ma Da Han School of Life Science Shanghai University institute of molecular Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine State Key Laboratory of Oncogenes and Related Genes Renji Hospital School of Medicine Shanghai Jiao Tong University Department of Research and Development Intellinosis Biotechnologies Co.Ltd.

Gastric cancer(GC) is a prevalent and lethal malignancy worldwide, and the 5-year survival rate is less than 30%. The absence of distinctive symptoms often leads to late-stage diagnosis, contributing to a grim prognosis. Early detection is pivotal for improving the survival rates and outcomes of GC patients. Traditional diagnostic methods, including gastroscopy and histopathological examination, are restricted in the early diagnosis of GC. Conventional protein biomarkers, such as carcinoembryonic antigen(CEA), carbohydrate antigen 19-9(CA19-9), and carbohydrate antigen 72-4(CA72-4), exhibit insufficient sensitivity and specificity for GC screening, which diminishes their clinical value. Recently, novel circulating biomarkers, such as circulating tumor cells(CTCs), circulating tumor cell DNA(ct DNA), and circulating free RNA(cf RNA), have emerged as promising candidates in the burgeoning field of liquid biopsy due to their superior sensitivity and specificity. This review focuses on the latest research on blood-based biomarkers for GC diagnosis and examines the clinical potentials and challenges associated with these emerging biomarkers.

关键词： gastric cancer early diagnosis blood

Effects of P301L-TAU on post-translational modifications of microtubules in human iPSC-derived cortical neurons and TAU transgenic mice

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2348-2360页

作者： Mohamed Aghyad Al Kabbani Christoph Köhler Hans Zempel institute of Human Genetics Faculty of Medicine and University Hospital CologneUniversity of CologneCologneGermany Center for molecular Medicine Cologne(CMMC) University of CologneCologneGermany Center Anatomy Department ⅡMedical FacultyUniversity of CologneCologneGermany

TAU is a microtubule-associated protein that promotes microtubule assembly and stability in the *** is missorted and aggregated in an array of diseases known as *** are essential for neuronal function and regulated via a complex set of post-translational modifications,changes of which affect microtubule stability and dynamics,microtubule interaction with other proteins and cellular structures,and mediate recruitment of microtubule-severing *** impairment of microtubule dynamics causes neuronal dysfunction,we hypothesize cognitive impairment in human disease to be impacted by impairment of microtubule *** therefore aimed to study the effects of a disease-causing mutation of TAU(P301L)on the levels and localization of microtubule post-translational modifications indicative of microtubule stability and dynamics,to assess whether P301L-TAU causes stability-changing modifications to *** investigate TAU localization,phosphorylation,and effects on tubulin post-translational modifications,we expressed wild-type or P301L-TAU in human MAPT-KO induced pluripotent stem cell-derived neurons(i Neurons)and studied TAU in neurons in the hippocampus of mice transgenic for human P301L-TAU(p R5 mice).Human neurons expressing the longest TAU isoform(2N4R)with the P301L mutation showed increased TAU phosphorylation at the AT8,but not the p-Ser-262 epitope,and increased polyglutamylation and acetylation of microtubules compared with endogenous TAU-expressing neurons.P301L-TAU showed pronounced somatodendritic presence,but also successful axonal enrichment and a similar axodendritic distribution comparable to exogenously expressed 2N4R-wildtype-TAU.P301L-TAU-expressing hippocampal neurons in transgenic mice showed prominent missorting and tauopathy-typical AT8-phosphorylation of TAU and increased polyglutamylation,but reduced acetylation,of microtubules compared with non-transgenic *** sum,P301L-TAU results in changes in micr

关键词： human induced pluripotent stem cell microtubules P301L pR5 mice TAU tauopathy tubulin code

维普期刊数据库

Mitosin/CENP-F is a conserved kinetochore protein subjected to cyto plasmic dynein-mediated poleward transport

在线全文

学校读者我要写书评

暂无评论

cell Research 2003年第4期13卷 275-283页

作者： ZHEN YE YANG, JING GUO, NING LI, MIN QIAN, SHENG NIAN WANG, XUE LIANG ZHULaboratory of molecular cell biology, institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores. We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine, canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint, thus implying a role of mitosin in this checkpoint. The

关键词： mitosin LEKl CENP-F kinetochore.

Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro lide

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2003年第4期13卷 295-300页

作者： YAN REN, LEI XIONG, JIA RUI WULaboratory of molecular cell biology, institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TC treated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c.

关键词： apoptosis mitochondrial pathway CHO cell.

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2005年第5期15卷 361-370页

作者： Yan JIN, Yong WEI, Lei XIONG, Ying YANG, Jia Rui WU* Laboratory of molecular cell biology, institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of sciences Shanghai China

Recent studies indicate that cell-cycle checkpoints are tightly correlated with the regulation of apoptosis, in which p53 plays an important role. Our present works show that the expression of E6/E7 oncogenes of human papillomavirus in HeLa cells is inhibited in the presence of anti-tumor reagent tripchlorolide (TC), which results in the up-regulation of p53 in HeLa cells. Interestingly, under the same TC-treatment, the cells at the early S-phase are more susceptible to apoptosis than those at the middle S-phase although p53 protein is stabilized to the same level in both situations. Significant difference is exhibited between the two specified expression profiles. Further analysis demonstrates that anti-apoptotic gene survivin is up-regulated by p53 in the TC-treated middle-S cells, whereas it is down-regulated by p53 in the TC-treated early-S cells. Taken together, the present study indicates that the differential p53-regulated expres- sion of survivin at different stages of the cell cycle results in different cellular outputs under the same apoptosis-inducer.

关键词： apoptosis cell cycle p53 survivin HeLa cell.

Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2004年第3期14卷 227-233页

作者： Yan REN, Jia Rui WU Laboratory of molecular cell biology, institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China. Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chkl checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern II was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly dete

关键词： intra-S-phase checkpoint DNA replication DNA damage tripchlorolide.

microRNAs: critical regulators in Th17 cells and players in diseases

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cellular & molecular Immunology 2010年第3期7卷 175-181页

作者： Bin Wei Gang Pei Laboratory of molecular cell biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina

microRNAs are a novel group of small,conserved,non-coding RNA molecules that are present in all *** molecules post-transcriptionally regulate gene expression by targeting mRNAs for degradation or by repressing the translation of the mRNAs.A good understanding of miRNA-mediated gene regulation is critical to gain a comprehensive view of many physiological processes and disease *** evidence demonstrates that miRNAs play an important role in the differentiation and function of the adaptive immune *** review provides an overview of the diverse functions of miRNAs in modulating immune responses and in immune cell development,particularly the development of Th17 cells,and explores the involvement of miRNAs in several autoimmune diseases including multiple sclerosis(MS),rheumatoid arthritis(RA),inflammatory bowel disease(IBD)and diabetes.

关键词： autoimmune diseases microRNAs Th17 cells

Functional analysis of two Sp1/Sp3 binding sites in murine Nanog gene promoter

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cell Research 2006年第3期16卷 319-322页

作者： Da Yong Wu Zhen Yao Laboratory of molecular cell biology,Laboratory of Stem cell biology,institute of Biochemistry and cell biology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences 320 Yue Yang Road,Shanghai 200031,China Laboratory of molecular cell biology Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Nanog gene plays a key role in maintaining pluripotency of ES cells and early embryonic cells.A 5’ flank sequenceof the Nanog gene has been reported to be regulated differentially,and two regulatory elements within the Nanog pro-moter,namely Oct-4 and Sox-2 binding sites,have been identified to regulate the transcriptional activity ofNanog *** this report,we identified the role of two putative Sp 1 binding sites located in the Nanog gene 5’-flanking region inregulation of murine Nanog gene *** studies showed that the two sites were essential for the Nanogpromoter *** shift and supershift analysis showed that both sites specifically bind Spl and ***,overexpression of dominant-negative Sp 1 or Sp3 mutants significantly inhibits Nanog promoter *** resultssuggest that the transcription factor Spl and Sp3 are important for Murine Nanog gene expression.

关键词： Nanog promoter Sp1 Sp3