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Analysis of an Adulterated Herbal Medicinal Product Using Ultra-Performance Liquid Chromatography Coupled with QTOF Mass Spectrometry

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World Journal of Traditional Chinese Medicine 2016年第3期2卷 1-9页

作者： Kate Yu Mark Powell Margaret Maziarz Dhavalkumar Narendrabhai Patel waters corporation Quay Pharmaceuticals Deeside Industrial Park Mark Powell Scientific waters Pacific Pte Ltd The CapricornSingapore Science Park Ⅱ

The reports of severe adverse effects and fatalities associated with herbal medicinal products adulterated with synthetic compounds have raised global *** objective of this study is to analyze one commercial herbal medicinal product suspected to be adulterated with synthetic drugs in order to identify potential adulterants,to verify if the product contained the herbs listed as ingredients in label claim and to determine quality consistency among different batches of the *** of suspected product obtained from seven different batches were performed using ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) with multiple data processing tools and multivariate *** addition,23 individual powdered herbs(12 as per label claim and 11 suspected herbs),11 marker compounds of the labeled herbs and five suspected synthetic drugs as adulterants were also concurrently analyzed to have clear understanding of product *** on our analysis,the major ingredients of studied product were found to be 5 synthetic compounds:caffeine,chlorphenamine,piroxicam,betamethasone and *** of them have been found to exceed their recommended *** the herbal composition analysis,Gan Cao(Glycyrrhizae radix et rhizoma) was found to be the main ingredient,which is not among the claimed 12 herbs that were supposed to be in the *** herbs detected as minor ingredients were Mu Gua(Chaenomelis fructus),Dang Gui(Angelicae sinensis radix),and Huang Qi(Astragali radix),which are among the 12 herbs that were supposed to be in the *** on our results we demonstrated that UPLC-QTOF MS is an effective and versatile tool for the analysis of herbal medicinal *** is highly desirable to have a streamlined process with automatic workflow and fit-forpurpose database to increase efficiency and productivity of sample *** of this work also highlight the need for the be

关键词： Synthetic adulterants Adulteration UPLC-QTOF-MS Herbal products Data independent acquisition Multi-Variate Statistical Analysis

APPLYING NOVEL ANALYTICAL TECHNOLOGIES TO FACE NATURAL PRODUCTS CHALLENGES

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World Journal of Traditional Chinese Medicine 2015年第4期1卷 73-74页

作者： Kate Yu Lirui Qiao Jing Huang waters corporation waters China Ltd

Herbal medicines have been used since ancient times for thetreatment of many diseases and *** chromatographic and spectroscopic techniques have had a tremendous impact on the analysis of herbal medicine to facilitate ... 详细信息

关键词： APPLYING NOVEL ANALYTICAL TECHNOLOGIES TO FACE NATURAL PRODUCTS CHALLENGES

DESI for high resolution MS imaging and REIMS for rapid real-time analysis of biological samples

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DESI for high resolution MS imaging and REIMS for rapid real...

2018年中国质谱学术大会（CMSC2018）

作者： Wei rao Zhengwei Jia waters corporation

Ambient MS involves the mass spectrometric analysis of samples with little to no pre-treatment, allowing rapid and in-situ examination of biological systems to be made. Both desorption electrospray ionization(DESI) and rapid evaporative ionization MS(REIMS) are ambient MS sources offered by waters that push the boundaries of this rapidly expanding field. DESI is a spray based technique that is capable of high spatial resolution MS imaging for many biological experiments, including tumour biomarker discovery, metabolite distribution in tissues, drug localization, plant samples, and many more. REIMS uses a diathermy knife to cause rapid heating of a sample, which then creates plumes of biological material that can be rapidly analyzed within a mass spectrometer. Together with LiveID software, this real-time collection of MS information allows near instantaneous determination of sample type. Here we present some of the latest advancements for these two ambient MS modalities with respect to technical improvements, sample handling, and novel applications.

关键词： DESI REIMS Ambient MS Biological Real time analysis

Assessing Performance and Method Transfer of Monoclonal Antibody and Peptide Bioseparation Methods using a novel Biocompatible uhplc system

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Assessing Performance and Method Transfer of Monoclonal Anti...

第六届生物技术药物理化特性分析与质量研究技术研讨会

作者： Brooke M.Koshel Ximo Zhang Robert Birdsall Corey Reed Stephan Koza Zhimin Li Paula Hong waters corporation

＜正＞Introduction The inherent complexity of biotherapeutic drugs requires more analytical methods,and methods capable of handling significant molecular complexity and *** date,many of the methods used in development and quality control laboratories remain *** this may be sufficient in some cases,there are notable advantages for updating legacy systems and

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Native ion Mobility mass Spectrometry for the Characterization of Biotherapeutics

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Native ion Mobility mass Spectrometry for the Characterizati...

第六届生物技术药物理化特性分析与质量研究技术研讨会

作者： Dale A.Cooper-Shepherd Jakub Ujma Kevin Giles Nick Tomczyk Laetitia Denbigh waters corporation

＜正＞Overview Here we present methods to characterize biotherapeutics using native-ion mobility spectrometry-mass ***-exclusion chromatography coupled to the Vion IMS QTof is a high erformance solution for native-IMS-MS analysis of ***-native IMS-MS has the potential to compare 3D structures of antibody *** MS under reducing conditions allows determination of intact antibody and subunits in one experiment.

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AN Integrated LC-MS Workflow for Subunit Core-fucosylation Characterization and Monitoring

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AN Integrated LC-MS Workflow for Subunit Core-fucosylation C...

第六届生物技术药物理化特性分析与质量研究技术研讨会

作者： Nilini Ranbaduge Henry Shion Ying Qing Yu Weibin Chen waters corporation

＜正＞IntroductionCore—fucosylation of N—glycans is a CQA that impacts the potency of monoclonal antibodies（mAbs）and can be monitored at subunit level.1-2Here,we demonstrate the performance of a workflow-driven LC-HRMS based assay in monitoring core-fucosylation of *** integrated subunit workflow in UNIFI is equipped with data acquisition,processing,and reporting capabilities to

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Rapid Digestion and Reproducible LC-MS Quantification of Cytochrome C: A Potential Biomarker for Apoptosis

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Rapid Digestion and Reproducible LC-MS Quantification of Cyt...

2018年中国质谱学术大会（CMSC2018）

作者： Paula Orens Mary Lame Erin Chambers waters corporation

Cytochrome C, is a mitochondrial protein(～13 kDa) which plays important roles in oxidative phosphorylation and apoptosis, or programmed cell death. Elevated plasma concentrations(～2 μg/mL) of circulating cytochrome C have been reported in patients with conditions associated with mitochondrial damage1. Use of LC-MS analysis for protein quantification has become more popular in the past few years due to the many benefits it offers(e.g., multiplexing, improved specificity, broader linear dynamic range, and faster method development times). However, for protein quantification by LC-MS, these protein digestion workflows are complex and time consuming, with enzymatic digestions often taking upwards of 24 hours to achieve sensitive and accurate quantification from complex biological matrices. Thus, We developed a rapid digestion and reproducible LC-MS method for the accurate quantification of cytochrome C in plasma. 35 μL plasma sample were digested for 10 minutes using ProteinWorks e Xpress Direct Digest Kit. Post digestion purification of signature peptides was completed using the ProteinWorks μElution SPE Clean-up Kit(15 minute SPE). Four unique tryptic peptide were identified: TGPNLHGLFGR, MIFAGIK, EDLIAYLK, and GITWGEETLMEYLENPKK. Chromatographic separation of cytochrome C tryptic peptides was achieved using a CORTECS UPLC C18+, 1.6 μm, 2.1 mm x 50 mm Column(5 minute method), which provides excellent peak shape, narrow peak widths(<3 secs at base), and resolution from matrix interferences. This method achieved excellent linearity and single digit RSDs(relative standard deviation) for duplicate calibration curves. The limit of quantification for Four unique signature tryptic peptide was 2 μg/ml. For all four peptides, at all QC levels, samples demonstrated excellent accuracy and precision with an average %CV of 2.5%. This work demonstrates proof of concept for the rapid, accurate and precise quantification of the protein biomarker, cytochrome C. The combination of the

关键词： cytochrome C protein quantification ProteinWorks μElution SPE CORTECS UPLC C18+

Automated Preparation of Labeled N-Glycans With a Vision-Guided Pipetting Robot for High Sensitivity LC-MS Analysis

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Automated Preparation of Labeled N-Glycans With a Vision-Gui...

第六届生物技术药物理化特性分析与质量研究技术研讨会

作者： Corey Reed Stephan Koza Jennifer Fournier Nikolas Vamvoukas waters corporation

＜正＞Introduction The GlycoWorks N-glycan analysis kit with RapiFluor-MS（RFMS）（Figure 1） labeling can provide significant time savings over reductive amination labeling methods such as 2-AB and provides sensitive...

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GC/MS/MS分析复杂基体中痕量组分的优越性

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环境化学 2005年第6期24卷 739-740页

作者： Keith Worrall Anthony Newton waters corporation Atlas Park Simonsway Manchester Waters Corporation Atlas Park Simonsway Manchester

在现代实验室中,GC/MS用于痕量分析极为普遍,要求更低的检测限而被分析样品的基体越来越复杂的情况由来已久.……[第一段]

关键词： GC/MS/MS 复杂基体痕量组分痕量分析分析样品实验室检测限