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Effects of symmetric and asymmetric salt conditions on a selective solid-state nanopore assay

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Nano Research 2022年第11期15卷 9936-9942页

作者： Ian D.Wadsworth Adam R.Hall virginia tech-wake forest university school of biomedical engineering and sciences Wake Forest School of MedicineWinston-SalemNC 27101USA Comprehensive Cancer Center Wake Forest School of MedicineWinston-SalemNC 27157USA

Conventional solid-state nanopore measurements sense all translocating entities,necessitating meticulous analysis to differentiate target *** address this,we have established a selective assay with the platform that has shown utility in quantifying several nucleic acid ***,limited detection efficiency and intrinsic noise have so far limited assay resolution to 10 *** in this value require manipulation of translocation ***,we report the effects of NaCl conditions on assay *** first investigate symmetric conditions,finding sensitivity increases with salt concentration but selectivity is maximized at 1.0 M *** then probe asymmetric conditions,showing a remarkable impact on assay sensitivity and selectivity when measurement buffer NaCl concentration in the reservoir with the translocating molecules is low and the opposite reservoir is *** optimum conditions,we demonstrate detection of target biomolecules down to a concentration of 100 pM which is an improvement of 2 orders of magnitude over past results.

关键词： solid-state nanopores biomarkers amplification-free detection salt gradient nucleic acids

BIOLUMINESCENCE TOMOGRAPHY:biomedical BACKGROUND,MATHEMATICAL THEORY,AND NUMERICAL APPROXIMATION

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Journal of Computational Mathematics 2008年第3期26卷 324-335页

作者： Weimin Han Ge Wang Department of Mathematics University of Iowa Iowa City IA 52242 USA Division of biomedical Imaging Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences Virginia Polytechnic Institute and State University Blacksburg Virginia 24061 USA

Over the last couple of years molecular imaging has been rapidly developed to study physiological and pathological processes in vivo at the cellular and molecular levels. Among molecular imaging modalities, optical imaging stands out for its unique advantages, especially performance and cost-effectiveness. Bioluminescence tomography （BLT） is an emerging optical imaging mode with promising biomedical advantages. In this survey paper, we explain the biomedical significance of BLT, summarize theoretical results on the analysis and numerical solution of a diffusion based BLT model, and comment on a few extensions for the study of BLT.

关键词： biomedical imaging Bioluminescence tomography (BLT) Inverse problem,Regularization Numerical approximation Error analysis

PGLYRP1 protein as a novel mediator of cellular dialogue in neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 1993-1994页

作者： Anup Bhusal Won-Ha Lee Kyoungho Suk Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea BK21 Plus KNU biomedical Convergence Program Department of Biomedical ScienceSchool of MedicineKyungpook National UniversityDaeguRepublic of Korea school of Life sciences BK21 plus KNU Creative BioResearch GroupKyungpook National UniversityDaeguRepublic of Korea Brain Science and engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Neuroinflammation has been identified as a crucial element in several neurological disorders. Glial cells play a critical role in directing neuroinflammation, both in deleterious and beneficial ways.

关键词： inflammation considerably

A functional tacrolimus-releasing nerve wrap for enhancing nerve regeneration following surgical nerve repair

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Neural Regeneration Research 2025年第1期20卷 291-304页

作者： Simeon C.Daeschler Katelyn J.W.So Konstantin Feinberg Marina Manoraj Jenny Cheung Jennifer Zhang Kaveh Mirmoeini JPaul Santerre Tessa Gordon Gregory HBorschel SickKids Research Institute Neuroscience and Mental Health ProgramTorontoONCanada Institute of Biomaterials and biomedical engineering University of TorontoTorontoONCanada Indiana university school of Medicine IndianapolisINUSA Division of Plastic and Reconstructive Surgery the Hospital for Sick ChildrenTorontoONCanada Translational Biology and engineering Program Ted Rogers Centre for Heart ResearchInstitute of Biomedical EngineeringFaculty of DentistryUniversity of TorontoTorontoONCanada

Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong ***,there are no FDA-approved therapies available to promote nerve *** accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve *** authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug *** is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 *** and drug loading are adjustable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible *** released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic *** its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.

关键词： biodegradable local drug delivery nerve injury nerve regeneration nerve wrap tacrolimus

Regulation of sleep by astrocytes in the hypothalamic ventrolateral preoptic nucleus

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Neural Regeneration Research 2025年第4期20卷 1098-1100页

作者： Jae-Hong Kim Ruqayya Afridi Il-Sung Jang Maan Gee Lee Kyoungho Suk Brain Korea 21 four KNU Convergence Educational Program of biomedical sciences for Creative Future Talents School of MedicineKyungpook National UniversityDaeguRepublic of Korea Department of Pharmacology School of MedicineKyungpook National UniversityDaeguRepublic of Korea Department of Pharmacology School of DentistryKyungpook National UniversityDaeguRepublic of Korea Brain Science&engineering Institute Kyungpook National UniversityDaeguRepublic of Korea

Astrocytes are functionally dynamic cells that support neurons in multiple ways throughout an organism’s *** astrocytic regulation of neuronal activity has been increasingly recognized in recent *** are now recognized as playing a more complex role than mere bystanders in the central nervous ***,their role in regulating the sleep neurocircuitry is not well *** this perspective,we highlight the role of astrocytes in sleep modulation,with a particular focus on regulatory mechanisms related to the ventrolateral preoptic nucleus(VLPO)of the *** briefly discuss recent literature reporting the role of VLPO astrocytes in regulating sleep-associated behaviors.

关键词： sleep ventrolateral astrocytes

engineering of Fe d-band center in Fe_(3)O_(4)/CeO_(2)hetero-nanoparticles via orbital coupling for high-efficiency oxygen reduction electrocatalysis

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Nano Research 2025年第1期18卷 129-139页

作者： Jiayi Liu Jingwen Yin Yingzi Lin Mingxin Pang Huan Pang Songtao Zhang Lin Xu Jun Yang Yawen Tang Jiangsu Key Laboratory of New Power Batteries Jiangsu Collaborative Innovation Center of Biomedical Functional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023Chin a 2 State Key Laboratory of Multiphase Complex Systems Center of MesoscienceInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China school of Chemistry and Chemical engineering Yangzhou UniversityYangzhou 225009China Testing Center Yangzhou UniversityYangzhou 225009China

The deliberate engineering of the d-band center of metal site represents an effective strategy to boost the intrinsic electrocatalytic performance toward the oxygen reduction reaction(ORR).Herein,following a heterointerfaceinduced orbital coupling rationale,we report a judicious design of an efficient ORR electrocatalyst consisting of Fe_(3)O_(4)/CeO_(2)hetero-nanoparticles in-situ encased into N-doped carbon nanofibers(abbreviated as Fe_(3)O_(4)/CeO_(2)@N-CNFs hereafter).The theoretic calculations uncover that the Fe_(3)O_(4)/CeO_(2)heterointerface-triggered orbital coupling can cause the down shift of the d-band center positions of Fe sites,which leads to the weakened chemisorption of oxygenated groups and lowered energy barrier for the potential-determining step,ultimately dramatically boosting the ORR intrinsic *** a consequence,the well-designed Fe_(3)O_(4)/CeO_(2)@N-CNFs display admirable ORR activity with a half-wave potential of 0.84 V and outstanding structural/electrochemical stability in an alkaline electrolyte,surpassing the commercial Pt/C benchmark and a majority of recently reported Fe_(3)O_(4)-based *** encouragingly,the Fe_(3)O_(4)/CeO_(2)@N-CNFsincorporated Zn-air battery outperforms the Pt/C-assembled counterpart with higher power density,larger energy density,and excellent cycling stability,serving as a competent candidate for ORR-involved renewable energy *** study offers an innovative approach for the rational manipulation of the d-band center and interfacial electron behavior of active sites toward the optimization of electrocatalytic performance.

关键词： hetero-nanoparticles electrospinning interfacial engineering orbital coupling oxygen reduction reaction

Characterization of Ablation Thresholds for 3D-Cultured Patient-Derived Glioma Stem Cells in Response to High-Frequency Irreversible Electroporation

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Research 2019年第1期2019卷 1005-1018页

作者： J.W.Ivey E.M.Wasson N.Alinezhadbalalami A.Kanitkar W.Debinski Z.Sheng R.V.Davalos S.S.Verbridge school of biomedical engineering and sciences Virginia Tech-Wake Forest UniversityBlacksburgVA 24061USA Department of Mechanical engineering Virginia TechBlacksburgVA 24061USA Brain Tumor Center of Excellence Comprehensive Cancer CenterWake Forest Baptist Medical CenterWinston-SalemNC 27157USA virginia tech Carilion Research Institute RoanokeVA 24061USA Department of Internal Medicine Virginia Tech Carilion School of MedicineRoanokeVA 24016USA Faculty of Health Science Virginia TechBlacksburgVA 24061USA

High-frequency irreversible electroporation(H-FIRE)is a technique that uses pulsed electric felds that have been shown to ablate malignant *** order to evaluate the clinical potential of H-FIRE to treat glioblastoma(GBM),a primary brain tumor,we have studied the efects of high-frequency waveforms on therapy-resistant glioma stem-like cell(GSC)*** demonstrate that patient-derived GSCs are more susceptible to H-FIRE damage than primary normal *** selectivity presents an opportunity for a degree of malignant cell targeting as bulk tumor cells and tumor stem cells are seen to exhibit similar lethal electric feld thresholds,signifcantly lower than that of healthy ***,neural stem cell(NSC)populations also exhibit a similar sensitivity to these *** observation may suggest that diferent considerations be taken when applying these therapies in younger versus older patients,where the importance of preserving NSC populations may impose diferent restrictions on *** also demonstrate variability in threshold among the three patient-derived GSC lines studied,suggesting the need for personalized cell-specifc characterization in the development of potential clinical *** work may provide further useful insights regarding this patient-dependent variability observed that could inform targeted and personalized treatment.

关键词： clinical younger irreversible

biomedical X-ray Imaging Enabled by Carbon Nanotube X-ray Sources

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Chinese Journal of Chemical Physics 2018年第4期31卷 529-536,615页

作者： Guohua Cao virginia tech-wake forest university school of biomedical engineering and sciences Virginia Poly.technic Institute and State University（Virginia Tech）BlacksburgVA 24061USA

Although discovered more than 100 years ago, X-ray source technology has evolved rather slowly. The recent invention of the carbon nanotube （CNT） X-ray source technology holds great promise to revolutionize the field of biomedical X-ray imaging. CNT X-ray sources have been successfully adapted to several biomedical imaging applications including dynamic rnicro-CT of small animals and stationary breast tomosynthesis of breast cancers. Yet their more irnportant biomedical imaging applications still lie ahead in the future, with the devel- oprnent of stationary rnulti-source CT as a noteworthy exarnple.

关键词： biomedical imaging X-ray imaging X-ray source Carbon nanotube X-raysource Carbon nanotube Field emission Cornputed tornography Breast tornosynthesis

Heat shock protein 90 inhibition by 17-DMAG lessens disease in the MRL/Ipr mouse model of systemic lupus erythematosus

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Cellular & Molecular Immunology 2012年第3期9卷 255-266页

作者： Samuel K Shimp III Cristen B Chafin Nicole L Regna Sarah E Hammond Molly A Read David L Caudell Marissa Nichole Rylander Christopher M Reilly virginia tech-wake forest school of biomedical engineering and Science Virginia Polytechnic Institute and State University Blacksburg VA USA Department of biomedical sciences and Pathobiology Virginia-Maryland Regional College of Veterinary Medicine Blacksburg VA USA Edward Via College of Osteopathic Medicine Blacksburg VA USA Department of Mechanical engineering Virginia Polytechinc Institue and State University Blacksburg VA USA

Elevated expression of heat shock protein 90 （HSP90） has been found in kidneys and serum of systemic lupus erythematosus （SLE） patients and MRLIMp-FasIprIFasJpr（MRLIIpr） autoimmune mice. We investigated if inhibition of HSP90 would reduce disease in MRL/ Ipr mice. In vitro, pretreatment of mesangial cells with HSP90 inhibitor Geldanamycin prior to immune-stimulation showed reduced expression of IL-6, IL-12 and NO. In vivo, we found HSP90 expression was elevated in MRL/Ipr kidneys when compared to C57BL/6 mice and MRIJIpr mice treated with HSP90 inhibitor 17-DMAG. MRIJIpr mice treated with 17-DMAG showed decreased proteinuria and reduced serum anti-dsDNA antibody production. Glomerulonephritis and glomerular IgG and C3 were not significantly affected by administration of 17-DMAG in MRIJIpr. 17-DMAG increased CD8＋ T cells, reduced double-negative T cells, decreased the CD4/CD8 ratio and reduced follicular B cells. These studies suggest that HSP90 may play a role in regulating T-cell differentiation and activation and that HSP90 inhibition may reduce inflammation in lupus.

关键词： Geldanamycin heat shock protein 90 MRUIpr systemic lupus erythematosus 17-DMAG