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Genome-wide CRISPR screens identify CLC-2 as a drug target for anti-herpesvirus therapy:tackling herpesvirus drug resistance

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Science China(Life Sciences) 2025年第2期68卷 515-526页

作者： Fayu Yang Nan Wei Shuo Cai Jing Liu Qingping Lan Hao Zhang Lu Shang Bo Zheng Mi Wang Yingchun Liu Lifang Zhang Chenzhong Fei Wu Tong Changlong Liu Ersheng Kuang Guangzhi Tong Feng Gu Shanghai veterinary research institute Chinese Academy of Agricultural SciencesShanghai 200241China Key Laboratory of veterinary Chemical Drugs and Pharmaceutics Ministry of Agriculture and Rural AffairsShanghai 200241China State Key Laboratory for Animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China institute of Human Virology Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China School of Medicine Tsinghua UniversityBeijing 100084China

The emergence of drug resistance to virus(i.e.,acyclovir(ACV)to herpesviruses)has been termed one of the common clinical issues,emphasizing the discovery of new antiviral *** address it,a genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)screening was performed in mouse haploid embryonic stem cells infected with pseudorabies virus(PRV),anα-herpesvirus causing human and pig *** results demonstrated that type 2 voltage-gated chloride channels(CLC-2)encoded by one of the identified genes,CLCN2,is a potential drug target for anti-herpesvirus ***-2 inhibitors,omeprazole(OME)and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid(DIDS),can efficiently inhibit infection of multiple herpesviruses in cellulo(i.e.,PRV,HSV and EBV),and effectively treat murine herpes simplex encephalitis(HSE).Additionally,DIDS was found to inhibit HSV-1 replication by blocking the PI3K/Akt *** importantly,both DIDS and OME were able to inhibit ACV-resistant HSV-1 strain *** study's findings suggest that targeting host-cell factors such as CLC-2 may be a promising approach to tackling herpesvirus drug *** discovery of CLC-2 as a potential drug target for anti-herpesvirus therapy provides a new direction for the development of novel antiviral agents.

关键词： herpesviruses CRISPR host-directed therapy CLC-2 DIDS

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Deletion of Salmonella pathogenicity islands SPI-1, 2 and 3 induces substantial morphological and metabolic alternation and protective immune potential

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Journal of Integrative Agriculture 2025年第1期24卷 272-289页

作者： Gaosong Liu Xuelian Lü Qiufeng Tian Wanjiang Zhang Fei Yi Yueling Zhang Shenye Yu State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

The Salmonella pathogenicity islands(SPIs) play crucial roles in the progression of Salmonella infection. In this study, we constructed an improved λ Red homologous recombination system to prepare single and triple deletion mutants of 3 prominent SPIs(SPI-1, 2, and 3), aiming at the impact of deletion on morphology, carbon source metabolism, adhesion and invasion capacity, in vivo colonization, and immune efficacy in chicks. Our examination revealed that the surface of the single deletion mutants(SM6ΔSPI1, ΔSPI2, and ΔSPI3) exhibited a more rugged texture and appeared to be enveloped in a layer of transparent colloid, whereas the morphology of the triple deletion mutant(SM6ΔSPI1&2&3) remained unaltered when compared to the parent strain. The carbon metabolic spectrum of the SPI mutants underwent profound alterations, with a notable and statistically significant modification observed in 30 out of 95 carbon sources, primarily carbohydrates(17 out of 30). Furthermore, the adhesion capacity of the 4 mutants to Caco-2 cells was significantly reduced when compared to that of the parent strain. Moreover,the invasion capacity of mutants SM6ΔSPI1 and SM6ΔSPI1&2&3 exhibited a substantial decrease, while it was enhanced to varying degrees for SM6ΔSPI3 and SM6ΔSPI2. Importantly, none of the 4 mutants induced any clinical symptoms in the chicks. However, they did transiently colonize the spleen and liver. Notably, the SM6ΔSPI1&2&3mutant was rapidly cleared from both the spleen and liver within 8 days post-infection and no notable pathological changes were observed in the organs. Additionally, when challenged, the mutants immunized groups displayed a significant increase in antibody levels and alterations in the CD3+CD4+ and CD3+CD8+ subpopulations, and the levels of IL-4 and IFN-γ cytokines in the SM6ΔSPI1&2&3 immunized chicken serum surpassed those of other *** summary, the successful construction of the 4 SPI mutants lays the groundwork for further exploration into the p

关键词： Salmonella pathogenicity islands(SPls) morphology carbon source metabolism pathogenicity immunogenicity live attenuated vaccine

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Reproductive aging in farm animals: Impacts on fertility and strategies for mitigation: A narrative review

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Asian pacific Journal of Reproduction 2025年第1期14卷 1-6页

作者： Akhter Rasool Sachin Irtiqa Manzoor Akshata Patil Division of Animal Reproduction Gynecology and ObstetricsNational Dairy research InstituteKarnalIndia Division of veterinary Medicine G.B.Pant University of Agriculture and TechnologyUttarakhandIndia Department of Animal Genetics and Breeding National Dairy research InstituteKarnalIndia

The livestock sector is a vital component of India's agricultural economy,providing essential support to rural communities and ensuring food *** reproductive performance is critical to sustaining livestock productivity,yet age-related declines in both male and female fertility pose significant *** current review highlights that male reproductive efficiency is closely tied to semen quality,which deteriorates with age due to oxidative stress,leading to reduced fertilizing capacity and embryonic developmental ***,female reproductive longevity is predominantly influenced by ovarian health,which declines with age due to diminished oocyte quality and *** decline is exacerbated by oxidative stress and mitochondrial dysfunction,leading to reproductive senescence and reduced offspring *** the mechanisms of reproductive aging and developing strategies to mitigate its effects are crucial for enhancing livestock productivity and sustaining the agrarian economy.

关键词： Sperm Oocyte Oxidative stress Mitochondrial dysfunction Reactive oxygen species Anti-oxidants

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Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential

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World Journal of Stem Cells 2025年第1期17卷 40-52页

作者： Seoung-Woo Lee Young-Ju Lim Hee-Yeon Kim Wansoo Kim Wook-Tae Park Min-Jung Ma Junho Lee Min-Soo Seo Young In Kim Sangbum Park Seong-Kyoon Choi Gun Woo Lee Core Protein Resources Center Daegu-Gyeongbuk Institute of Science and TechnologyDaegu 42988South Korea Department of Orthopedic Surgery Yeungnam University Medical CenterYeungnam University College of MedicineDaegu 42415South Korea Department of veterinary Pathology College of Veterinary MedicineKyungpook National UniversityDaegu 41566South Korea School of Life Science BK21 FOUR KNU Creative BioResearch GroupKyungpook National UniversityDaegu 41566South Korea Laboratory of veterinary Tissue Engineering College of Veterinary MedicineKyungpook National UniversityDaegu 41566South Korea College of veterinary Medicine Kyungpook National UniversityDaegu 41566South Korea Department of veterinary Tissue Engineering Kyungpook National UniversityDaegu 41566South Korea Cellexobio. Ltd.Daegu 42415South Korea Michigan State University Institute for Quantitative Health Science&EngineeringEast LansingMI 48824United States Department of Orthopedic Surgery Yeungnam University College of MedicineDaegu 42415South Korea

BACKGROUND Mesenchymal stem cells(MSCs)are promising candidates for regenerative therapy due to their self-renewal capability,multilineage differentiation potential,and immunomodulatory *** molecular characteristics of MSCs are influenced by their ***,epidural fat(EF)and EF-derived MSCs(EF-MSCs)have garnered attention due to their potential benefits to the spinal microenvironment and their high expression of neural SC ***,their clinical applications are limited due to cell senescence and limited accessibility of *** many studies have attempted to establish an immortalized,stable SC line,the characteristics of immortalized EF-MSCs remain to be *** To establish and analyze stable immortalized *** The phenotypes of EF-MSCs were analyzed using optical *** immortalization was performed using lentiviral *** biomolecular characteristics of the cells were analyzed by immunoblotting,quantitative PCR,and *** The immortalized EF-MSCs demonstrated a significantly extended lifespan compared to the control group,with well-preserved adipogenic potential and SC surface marker *** of human telomerase reverse transcriptase genes markedly increased the lifespan of *** analysis revealed substantial increase in the expression of DNA replication pathway components in immortalized *** Immortalized EF-MSCs exhibited significantly enhanced proliferative capacity,retained adipogenic potential,and upregulated the expression of DNA replication pathway components.

关键词： Immortalization Transfection Stem cell Epidural fat Proteomics

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Dietary supplementation with citrus peel extract in transition period improves rumen microbial composition and ameliorates energy metabolism and lactation performance of dairy cows

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Journal of Animal Science and Biotechnology 2025年第1期16卷 441-454页

作者： Lingxue Ju Qi Shao Zhiyuan Fang Erminio Trevisi Meng Chen Yuxiang Song Wenwen Gao Lin Lei Xinwei Li Guowen Liu Xiliang Du State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun 130062China Department of Animal Sciences Food and NutritionFaculty of AgricultureFood and Environmental ScienceUniversitàCattolica del Sacro CuorePiacenza 29122Italy

Background During the transition period,excessive negative energy balance(NEB)lead to metabolic disorders and reduced milk *** microbes are responsible for resolving plant material and producing volatile fatty acids(VFA),which are the primary energy source for *** this study,we aimed to investigate the effect of citrus peel extract(CPE)supplementation on rumen microbiota composition,energy metabolism and milk performance of peri-partum dairy *** Dairy cows were fed either a basal diet(CON group)or the same basal diet supplemented with CPE via intragastric administration(4 g/d,CPE group)for 6 weeks(3 weeks before and 3 weeks after calving;n=15 per group).Samples of serum,milk,rumen fluid,adipose tissue,and liver were collected to assess the effects of CPE on rumen microbiota composition,rumen fermentation parameters,milk performance,and energy metabolic status of dairy *** CPE supplementation led to an increase in milk yield,milk protein and lactose contents,and serum glucose levels,while reduced serum concentrations of non-esterified fatty acid,β-hydroxybutyric acid,insulin,aspartate aminotransferase,alanine aminotransferase,and haptoglobin during the first month of *** supplemen-tation also increased the content of ruminal *** to the CON group,the abundance of Prevotellaceae,Methanobacteriaceae,Bacteroidales_RF16_group,and Selenomonadaceae was found increased,while the abun-dance of Oscillospiraceae,F082,Ruminococcaceae,Christensenellaceae,Muribaculaceae UCG-011,Saccharimona-daceae,Hungateiclostridiaceae,and Spirochaetaceae in the CPE group was found *** adipose tissue,CPE supplementation decreased lipolysis,and inflammatory response,while increased insulin *** the liver,CPE supplementation decreased lipid accumulation,increased insulin sensitivity,and upregulated expression of genes involved in *** Our findings suggest that CPE supplementation during the peripartum period

关键词： Adipose tissue Citrus peel extract Energy metabolism Liver Rumen microbiota

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From mice to humans:a need for comparable results in mammalian neuroplasticity

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Neural Regeneration research 2025年第2期20卷 464-466页

作者： Marco Ghibaudi Enrica Boda Luca Bonfanti Neuroscience institute Cavalieri Ottolenghi OrbassanoItaly Department of veterinary Sciences University of TurinTurinItaly Department of Neuroscience Rita Levi-Montalcini University of TurinTurinItaly

Brain plasticity-A universal tool with many variations:The study of brain plasticity has been gaining interest since almost a century and has now reached a huge amount of information(>80,000 results in PubMed).Overall,different types of plasticity,including stem cell-driven genesis of new neurons(adult neurogenesis),cells in arrested maturation(dormant neurons),neuro-glial and synaptic plasticity,can coexist and contribute to grant plastic changes in the brain,from a cellular to system level(Benedetti and Couillard-Despres,2022;Bonfanti et al.,2023).

关键词： plasticity al. arrest

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On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay

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Journal of Integrative Agriculture 2025年第2期24卷 790-794页

作者： Kaikai Jin Junjie Zhao Huanxin Chen Zimo Zhang Zengguo Cao Zanheng Huang Hao Li Yongsai Liu Lisi Ai Yufei Liu Changqi Fan Yuanyuan Li Pei Huang Hualei Wang Haili Zhang State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis research of the Ministry of Education/institute of Zoonosis College of Veterinary Medicine Jilin University National/WOAH Reference Laboratory for Classical Swine Fever China Institute of Veterinary Drug Control Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of Virology Center for Biosafety Mega-Science Chinese Academy of Sciences Zhili College Tsinghua University

Highlights The RT-RAA-VF assay developed for the NiV P gene can perform rapid detection of NiV within 20 min at 42°C with high *** assay is capable of attaining sensitivity to a single copy of NiV RNA *** assay effectively avoids false positives caused by aerosol contamination with a sealed disposable nucleic acid visualization test paper device.

关键词： dipstick visual detection assay virus with aided amplification combining lateral-flow

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An ionic liquid assisted hydrogel functionalized silica stationary phase for mixed-mode liquid chromatography

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Chinese Chemical Letters 2025年第1期36卷 491-495页

作者： Tong Zhang Xiaojing Liang Licheng Wang Shuai Wang Xiaoxiao Liu Yong Guo Lanzhou institute of Chemical Physics Chinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China Lanzhou institute for Food and Drug Control Key Laboratory of Pesticide and Veterinary Drug Monitoring for State Market RegulationLanzhou 730050China

An ionic liquid assisted hydrogel modified silica was synthesized using a one-pot polymerization and physical coating technique and subsequently applied to mixed-mode liquid *** techniques,including Fourier transform infrared spectroscopy(FT-IR),X-ray photoelectron spectroscopy(XPS),and elemental analysis,etc.,confirmed the successful prepared of this innovative stationary *** unique combination of amide,long alkyl chain,and imidazole ring in the hydrogel coating enables the stationary phase to function effectively in hydrophilic/reversed-phase/ion exchange liquid ***,the stationary phase exhibited superior separation performance owing to the synergistic effect of the ionic liquid and *** was particularly evident when analyzing various analytes such as organic acids,nucleosides/bases,polycyclic aromatic hydrocarbons(PAHs)and ***,under our operating conditions,an excellent column efficiency of 53,642.9 plates/m was achieved for *** summary,we have proposed a straightforward strategy to enhance the separation performance of hydrogel coatings in liquid chromatography,thereby broadening the potential applications of hydrogels in the field of separation.

关键词： Ionic liquid Hydrogel Functionalized silica Synergistic effect Stationary phase

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Physiological and agronomic effects of regulated-deficit irrigation on soybean grown under arid climatic conditions

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The Crop Journal 2025年第1期13卷 281-291页

作者： Bouthayna El Amine Fatema Mosseddaq Abdelhadi Ait Houssa Ahmed Bouaziz Lhoussaine Moughli Abdallah Oukarroum Hassan II institute of Agronomy and veterinary Medicine Department of Crop ProductionProtection and BiotechnologyRabat 10100Morocco Mohammed VI Polytechnic University Plant Stress Physiology LaboratoryAgrobiosciencesBenguerir 43150Morocco Providence Verte Company Agricultural Training and Research CenterRabat 10020Morocco

Drought is one of the most severe environmental stresses affecting soybean growth and development,especially in arid and semi-arid *** aim of this experiment is to evaluate the effect of regulated deficit irrigation during the vegetative stages on soybean plants and determine the amount irrigation water can be reduced without affecting the physiological parameters,the crop phenology,and the yield of the soybean *** field experiments were conducted during two irrigation crop seasons(2021 and 2022)in Louata,*** results showed that regulated deficit irrigation regimes during the vegetative stages was combined with high temperatures and low air humidities during the beginning of flowering and the pod filling stage during 2021 in comparison with 2022,especially for 25%CWR(crop water requirements).Regulated deficit irrigation regimes reduced the stomatal conductance by 46%and 52%respectively during the first and second growing seasons by limiting CO2 intake for the Calvin *** stomata closure increased the leaf temperature and affected the functioning of the photosynthetic apparatus by damaging the chlorophyll pigments and impairment of electron transport chains in *** transition from regulated deficit irrigation to 100%CWR at the beginning of flowering(R1)compensated for the photosynthetic loss,improved the growth and development of soybean plants and enhanced the yield and its components for 50%and 75%*** adaptative mechanism such as the remobilization of the carbon reserved in the stems and leaves(vegetative tissues)to the grains improved the grain yield by 36.7%during 2021 and by 32.2%during 2022 *** consequently improved the water use efficiency,the water productivity of soybean for 50%and 75%CWR and contributed to water saving with an average of 60 mm per growing season.

关键词： Soybean Regulated-deficit irrigation Vegetative phase Soil water content Plant physiology and yield

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A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells

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无机化学学报 2025年第3期41卷 540-548页

作者： ZHANG Linfang YIN Wenzhu YIN Gui School of Chemistry and Chemical Engineering Yili Normal UniversityYiningXinjiang 835000China institute of veterinary Immunology&Engineering National Research Center of Engineering and Technology for Veterinary BiologicalsJiangsu Academy of Agricultural SciencesNanjing 210014China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran(TCF)as a near-infrared fluorescent chromophore,we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H_(2)*** probe TCF-NS displayed a rapid-response fluorescent against H_(2)S with high sensitivity and selection but had no significant fluorescence response to other ***,TCF-NS was applied to sense H_(2)S in living cells successfully with minimized cytotoxicity and a large Stokes shift.

关键词： hydrogen sulfide near⁃infrared fluorescence probe cell imaging

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