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Horticultural Plant Journal 2020年第5期6卷 313-320页

作者： Yanxia Shi Zhiwen Jin Xianglong Meng Lixue Wang Xuewen Xie Ali Chai Baoju Li Institute of vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China

Pectobacterium carotovorum is the causal agent of bacterial soft rot in a wide range of vegetable host *** *** infects the plant,the spread of the disease is difficult to *** this study,a rapid and sensitive method based on loop-mediated isothermal amplification(LAMP)was developed for detecting *** in celery with soft rot using a primer set designed from the pmrA conserved sequence of *** specificity of the LAMP primer set for *** was extensively validated on both *** strains and nontarget *** sensitivity was 1 pg of *** genomic DNA,which demonstrated 10 times more sensitive than the conventional PCR *** was also used to detect *** in bacterial *** lowest detection concentration was 104 CFU·mL^−*** addition,a LAMP assay,in conjunction with a crude DNA extraction method,was successfully performed on ***-infected samples derived from both artificially and naturally infected *** summary,the LAMP assay established in this study constitutes a simple,sensitive,and rapid method for the detection of ***,and has potential application in the control of celery soft rot disease through early detection.

关键词： Bacterial soft rot P.carotovorum Loop-mediated isothermal amplification(LAMP) celery pmrA gene field detection

Influences of Genotype and Explant on Callus Induction and Shoot Regeneration in Sunflower （Helianthus annuus L.）

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Journal of Agricultural science and Technology 2011年第2期5卷 226-231页

作者： M. Bayraktaroglu N. Dagustu department of field crops Faculty of Agriculture Uludag University Gorukle Bursa 16059 Turkey

In vitro regeneration was performed with the aim of developing efficient callus and shoot regeneration from different explants of sunflower （Helianthus annuus L.）. The 6 genotypes （RHAI0, RHA 14, RHA15, PR6404, N Record 109/Sanay 3-5, N Record 109/Sera） were used as plant materials. The roots, hypocotyls and cotyledons were excised from 4 day-old seedlings and cultured on embryo induction medium （EIM） supplemented with Benzylaminopurine （BAP）, Naphthaleneacetic acid （NAA） and Gibberellic acid （GA3）. The experiments were kept in 18/6 hour light/dark photoperiod at 26± 2 ℃for four weeks. The rates of callus and root organ formation on explants were 67%-100% and 7%-31% respectively, depending on the genotype. Root explants produced statistically high callus formation （2.6 score） compared to cotyledon （2.0 score） and hypocotyl explants （2.5 score） at all sunflower genotypes used. The highest shoot regeneration was obtained from RHAI 5 （7%） while PR6404 （100%） produced the highest callus formation.

关键词： Sunflower (Helianthus annuus L.) genotype explant callus induction shoots regeneration.

Potential of fungal thermostable alpha amylase enzyme isolated from Hot springs of Central Anatolia(Turkey)in wheat bread quality

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Food Bioscience 2022年第1期45卷 214-222页

作者： Arzu Unal Asiye Seis Subasi Semra Malkoc Ijlal Ocak SElif Korcan Elif Yetilmezer Seyhun Yurdugül Hülya Yaman Turgay Sanal Alaettin Keceli Igdır University Faculty of AgricultureDepartment of Agricultural BiotechnologyI˘gdırTurkey Ministry of Agriculture and Forestry field crops Central Research Institute Department of Quality and TechnologyAnkaraTurkey Eskisehir Technical University Faculty of EngineeringDepartment of Environmental EngineeringEskisehirTurkey Afyon Kocatepe University Faculty of EducationDepartment of Mathematics and Science EducationDivision of Science EducationAfyonTurkey Usak University Vocational School of Health ServicesMedical Laboratory Techniques ProgramUsakTurkey Ministry of Agriculture and Forestry field crops Central Research Institute Department of BiotechnologyAnkaraTurkey Bolu Abant Izzet Baysal University Faculty of Arts and ScienceDepartment of BiologyBoluTurkey Bolu Abant Izzet Baysal University Vocational School of the Technical Sciences of BoluFood Technology ProgramBoluTurkey Pamukkale University School of Applied SciencesDepartment of Organic Farming Business ManagementDenizliTurkey

Many industrial processes may quickly proceed with biotechnological and enzymatic *** and production facilitator properties of enzymes have provided variable advantages for ***,enzyme utilization has become inevitable for industry and supplied financial advantages in case of the high energy requirement *** the study,the amylase enzymes were isolated from thermal spring sources,and the effect on bread quality was ***,fungal amylases were isolated from thermal spring sources(29-98◦C)in various places around *** determining the functional properties of amylase enzymes,the most active enzyme was used in bread production to examine their effect on bread *** maximum alpha-amylase activity(38.6 U/mg)has been detected in Aspergillus niger G 2-1 *** compared with the commercially available one,native alpha-amylase increased the bread volume.A significant difference(p<0.01)was found in the color properties and size of the bread between microbial produced in this study and commercially produced *** ppm alpha-amylase addition showed the optimum bread properties for dough processing.

关键词： Alpha-amylase Thermal spring Quality of bread Commercial enzymes

Bio-capped and green synthesis of ZnO/g-C_(3)N_(4) nanocomposites and its improved antibiotic and photocatalytic activities: An exceptional approach towards environmental remediation

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Chinese Journal of Chemical Engineering 2023年第4期56卷 215-224页

作者： Iltaf Khan Chunjuan Wang Shoaib Khan Jinyin Chen Aftab Khan Sayyar Ali Shah Aihua Yuan Sohail Khan Mehwish KButt Humaira Asghar School of Environmental&Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China College of Agriculture Shihezi UniversityShihezi 832003China Key Laboratory of Biology and Genetic Improvement of Horticultural crops Northeast Agricultural UniversityHarbin 150030China Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits and vegetables College of AgronomyJiangxi Agricultural UniversityNanchang 330045China department of Pharmacy University of SwabiKhyber Pakhtunkhwa 94640Pakistan department of Physics University of Agriculture FaisalabadFaisalabad 38040Pakistan department of Chemistry Government College University FaisalabadFaisalabad 38000Pakistan

In this research study, we have synthesized the bio-capped ZnO/g-C_(3)N_(4) nanocomposites by employing lemon juice(Citrus limon) as a stabilizer and mediator. Fruitfully, lemon juice which contains various acidic functional groups and citric acid has the capability to block the surface of g-C_(3)N_(4) from chemical reactivity and activated the surface of g-C_(3)N_(4) for various reactions. Consequently, the agglomeration behavior and controlled shape of g-C_(3)N_(4) has also been achieved. Our experimental results i.e. XRD,TEM, HRTEM, PL, FS, XPS, and PEC have confirmed that the lemon juice mediated and green g-C_(3)N_(4)(L-CN) have good performances and remarkable visible light photocatalytic activities as compared to the chemically synthesized g-C_(3)N_(4)(CN). Furthermore, the small surface area and low charge separation of g-C_(3)N_(4) is upgraded by coupling with Zn O nanoparticles. It is proved that the coupling of Zn O worked as a facilitator and photoelectron modulator to enhance the charge separation of g-C_(3)N_(4). Compared to pristine lemon-mediated green g-C_(3)N_(4)(L-CN), the most active sample 5Zn O/L-CN showed ~ 5-fold improvement in activities for ciprofloxacin(CIP) and methylene blue(MB) degradation. More specifically,the mineralization process and degradation pathways, and the mineralization process of ciprofloxacin(CIP) and methylene blue(MB) are suggested. Finally, our present novel research work will provide new access to synthesize the eco-friendly and bio-caped green g-C_(3)N_(4)nanomaterials and their employment for pollutants degradation and environmental purification.

关键词： Bio-caped green g-C_(3)N_(4) Lemon juice mediators ZnO/g-C_(3)N_(4)nanocomposite Environmental remediation Degradation pathways

Identification of Genetically Distinct Cassava Clones from On-Farm Plantations to Widen the Thai Cassava Breeding Gene Pool

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American Journal of Plant sciences 2013年第8期4卷 1574-1583页

作者： Preeya P. Wangsomnuk Benjawan Ruttawat Prapit Wongtiem department of Biology Faculty of Science Khon Kaen University Khon Kaen Thailand Rayong field crops Research Center Department of Agriculture Rayong Thailand.

Cassava (Manihot esculenta Crantz) is one of the most important economic crops in Thailand. However, the Thai cassava breeding gene pool was genetically narrow with only 11 distinct landraces. An attempt was made here to characterize 266 cassava clones collected from 80 farms in eight provinces using 35 SSR markers. A total of 365 polymorphic alleles were detected in the assayed samples. The molecular analysis of variance revealed that a large SSR variance (19.8%) was present among the farm samples. The genetic relationships of the 266 farm samples revealed by the principal coordinate analysis confirmed the large SSR variation observed among the collected cassava samples. The average dissimilarity (AD) of a cassava sample against the other 265 samples was calculated and the AD values obtained ranged from 0.256 to 0.502 with a mean of 0.319. Based on these AD values, a set of 50 unique cassava samples with AD values of 0.346 or higher was assembled from the on-farm samples to widen the genetic base of the Thai cassava breeding gene pool.

关键词： Cassava Distinctness SSR Ex Situ Conservation Core Collection

OsPDR20 is an ABCG metal transporter regulating cadmium accumulation in rice

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Journal of Environmental sciences 2024年第2期136卷 21-34页

作者： He Li Chao Li Di Sun Zhi Min Yang department of Biochemistry and Molecular Biology College of Life SciencesNanjing Agricultural UniversityNanjing 210095China

Cadmium(Cd)is a non-essential toxic heavy metal,seriously posing high environmental risks to human *** genetic resources relevant to functional genes is important for understanding the metal absorption and accumulation in crops and bioremediation of Cd-polluted *** study investigated a functionally uncharacterized ATP binding cassette transporter G family(ABCG)gene encoding a Pleiotropic Drug Resistance 20(PDR20)type metal transporter which is localized to the plasma membrane of ***20 was transcriptionally expressed in almost all tissues and organs in lifespan and was strongly induced in roots and shoots of young rice under Cd *** expression of OsPDR20 in a yeast mutant ycf1 sensitive to Cd conferred cellular tolerance with less Cd *** of OsPDR20 by RNA interference(RNAi)moderately attenuated root/shoot elongation and biomass,with reduced chlorophylls in rice grown under hydroponic medium with 2 and 10μmol/L Cd,but led to more Cd accumulation.A field trial of rice grown in a realistic Cd-contaminated soil(0.40 mg/kg)showed that RNAi plants growth and development were also compromised compared to wild-type(WT),with smaller panicles and lower spikelet fertility but little effect on yield of ***,OsPDR20 suppression resulted in unexpectedly higher levels of Cd accumulation in rice straw including lower leaves and culm and *** results suggest that OsPDR20 is actively involved in Cd accumulation and homeostasis in rice *** increased Cd accumulation in the RNAi plants has the potential application in phytoremediation of Cd-polluted wetland soils.

关键词： Rice ABCG transporter Cadmium accumulation Phytoremediation

Estimation of Some Plant Secondary Products in Urtica dioica L., Viola odorata L. and Melissa officinalis L. Naturally Grown in Hawraman-Kurdistan Region of Iraq

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Journal of Agricultural science and Technology(B) 2013年第7期3卷 480-486页

作者： Abdulsalam Abdulrahman Rasool Kadhm Abdullah Muhammad field crops department Faculty of Agricultural Sciences University of Sulaimani Sulaimani Kurdistan Region Iraq

The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations： Biare 1,090 masl （meters above sea level）, Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations： Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components （fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components （violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils （pinene, linalool, citronellol, geraniol and rosmarinic acid）. Their quantitative evaluations were influenced by altitudes indic

关键词： Urtica dioica L. Viola odorata L. Melissa officinalis L. altitudes secondary products medicinal plants

The Effect of Zinc （Zn） Fertilization on Alleviating Cd Accumulation in Durum Wheat Grain

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Journal of Agricultural science and Technology(B) 2018年第4期8卷 203-208页

作者： Faruk Ozkutlu Sevket Metin Kara department of Soil science and Plant Nutrition Faculty of Agriculture Ordu University Ordu 52200 Turkey department of field crops Faculty of Agriculture Ordu University Ordu 52200 Turkey

Cadmium （Cd） contamination of agricultural soils may pose severe risks and hazards for humans through food chain, as crop plants accumulate Cd in their edible tissues. Cd translocation from soil to plant is largely dependent on soil and plant type. Cd accumulation occurs much more in crop plants grown in soils with severe zinc （Zn） deficiency and durum wheat tends to accumulate more Cd in grain than the other cereals. The objective of this study was to evaluate the alleviating effect of Zn fertilization on Cd accumulation in durum wheat grain. A pot experiment including foliar Zn application of 0.3% w/v ZnSO4 and soil Cd applications of 0, 0.2 and 1.0 mg/kg was carried out in a completely randomized design using a Zn-deficient soil. Grain Cd concentration of plants receiving 0 mg/kg Cd was 31 μg/kg, whereas with 0.2 mg/kg and 1.0 mg/kg Cd applications it increased to the levels of 215 μg/kg and 1,489 μg/kg, respectively. Along with 0.3% ZnSO4 leaf applications, grain Cd concentrations decreased to 171 μg/kg and 754 μg/kg, by a reduction of 20.5% and 49.3%, respectively. In conclusion, it was determined that leaf applied Zn fertilizer might alleviate Cd accumulation in durum wheat grain in Zn deficiency conditions.

关键词： Cadmium Triticum turgidum L. durum Zn deficiency Zn-deficient soil.

Mycorrhiza and Phosphate Solubilizing Bacteria:Potential Bioagents for Sustainable Phosphorus Management in Agriculture

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Phyton-International Journal of Experimental Botany 2022年第2期91卷 257-278页

作者： Fazli Wahid Muhammad Sharif Shah Fahad Amjad Ali Muhammad Adnan Rafiullah Shah Saud Subhan Danish Muhammad Arif Ali Niaz Ahmed Hüseyin Arslan Doğan Arslan Murat Erman Ayman EL Sabagh Fatemeh Gholizadeh Rahul Datta department of Agriculture University of SwabiSwabi23561Pakistan department of Soil and Environmental sciences The University of AgriculturePeshawar25130Pakistan department of Agronomy The University of HaripurHaripur22620Pakistan School of Environmental and Municipal Engineering Xi’an University of Architecture and TechnologyXi’an710055China department of Horticulture Northeast Agricultural UniversityHarbin150030China department of Soil science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultan60800Pakistan department of field crops Faculty of AgricultureHarranÜniversityOsmanbey CampusŞanliurfa63050Turkey department of field crops Faculty of AgricultureSiirt UniversitySiirt56100Turkey department of Agronomy Faculty of AgricultureKafrelsheikh UniversityKafrelsheikh33156Egypt department of Plant Production and Genetics Faculty of AgricultureUniversity of KurdistanSanandaj66177-15175Iran department of Geology and Pedology Faculty of Forestry and Wood TechnologyMendel University in BrnoBrno61300Czech Republic

Phosphorus(P)is a critical nutrient that plays an essential role in improving soil fertility for optimum plant growth and *** is one of the most deficient macro-nutrients in agricultural soils after nitrogen and is considered inadequate for plant growth and *** P availability in soils,the farmers are applying huge amounts of synthetic P fertilizers that adversely affect the wider environment,groundwater,soil fertility and microbial *** beneficial microbes are known to release and supply soluble P for improving growth and yield of a variety of plants in a sustainable manner in P deficient ***,inoculation of these microbes,including arbuscular mycorrhizal fungi(AMF)and phosphate solubilizing bacteria(PSB)to soil to enhance crop production without harming the environment,is an alternative approach to chemical *** combined role of AMF and PSB in P solubilization is not well understood and the application and mode of action of these microbial groups are often naive due to variation in the ***,the current review article would develop a better understanding of the interactive role and mechanisms of AMF and PSB in improving P availability from both organic and inorganic sources in a sustainable crop production ***,the current review would loop out further avenues for researchers interested to commercially produce effective AMF and PSB-based biofertilizers for sustainable management of phosphorus over a wide range of agricultural crops worldwide.

关键词： Beneficial microbes soil inoculation phosphorus mineralization crop production

Responses of Antioxidant Enzymes to Chilling Stress in Tobacco Seedlings

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Agricultural sciences in China 2010年第11期9卷 1594-1601页

作者： XU Sheng-chun LI Yong-ping HU Jin GUAN Ya-jing MA Wen-guang ZHENG Yun-ye ZHU Shui-jin department of Agronomy Zhejiang University Hangzhou 310029 P.R.China Tobacco Agricultural science Research Academy of Yunan Province Yuxi 653100 P.R. China Institute of vegetables Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R. China

Chilling is one of the major abiotic stresses limiting yield and quality of many important crops. For better understanding of chilling stress responses in tobacco （Nicotiana tabacum）, growth rate and antioxidant enzymes of seedlings in 2 tobacco cultivars, viz., MSk326 （chilling sensitive variety） and Honghuadajinyuan （HHDJY, chilling tolerant variety） at chilling temperature （5℃） were studied. The results showed that the relative growth rate in chilling period to that in recovery period was significantly higher in roots than that in shoots for both cultivars, suggesting that shoots growth was more easily affected by chilling stress. Chilling stress increased peroxidase （POD） activity and reduced superoxide dismutase （SOD） activity in shoots of HHDJY, and catalase （CAT） activity was little affected. In the roots of HHDJY, chilling stress increased SOD and CAT activities, and had little effect on POD activity. For MSk326, chilling treatment increased SOD activity in shoots and declined CAT activity in roots. MDA concentration in both varieties was increased under the chilling stress, while it was decreased after seedlings were recovered growth for 4 d at normal temperature （25℃）. It showed that tobacco seedlings might have the capacity of recovering from chilling injury for a short term, The relationship between the growth rate and antioxidant enzyme activity was analyzed by stepwise regression. It was found that there was a close relationship between relative growth rate of tobacco seedlings and CAT activity under chilling stress condition and regression equations containing CAT could be used in predicting seedling growth rate of tobacco under chilling stress condition.

关键词： antioxidant enzymes chilling stress stepwise regression tobacco