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Molecular characterisation and phylogenetic analysis of Spirometra erinaceieuropaei from snakes in Hunan Province

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Molecular characterisation and phylogenetic analysis of Spir...

Cancer immunotherapy with envelopedself-amplifying mRNA CARG-2020 thatmodulates IL-12,IL-17 and PD-L1 pathways toprevent tumor recurrence

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： TF Gong LX Xie JL He F Li SY Chen WC Li XR Xie YS Liu YF Zhou W Liu research center for Parasites & vectors College of Veterinary MedicineHunan Agricultural University Orient Science & Technology College of Hunan Agriculture University

Sparganosis is a neglected zoonotic parasitic disease found *** play an important role in sparganosis transmission because they are the most common second intermediate hosts for Spirometra ***,the population genetics of Spirometra isolates from snakes is currently not well *** study obtained 49 Spirometra tapeworms from 15 geographical areas in Hunan Province,Central ***,18 S and 28 S ribosomal DNA(rDNA) fragments were amplified from the isolated parasites,and their sequences were analysed to assess their genetic *** analyses were performed using the maximum likelihood *** results showed that sequence variations amongst these isolates were 0%-2.3% and 0%-0.1% for 18 S and 28 S rDNA,*** analysis showed that all Spirometra isolates from Hunan Province were clustered into the same branch with *** isolated from different regions/hosts ***,phylogenetic trees revealed that Spirometra is closely related to Adenocephalus,Pyramicocephalus,Ligula,Dibothriocephalus,Schistocephalus and *** results provide reference values for the future taxonomic status of ***,and the exact phylogenetic position of Spirometra species should be further analysed with more sampling and more useful molecular markers.

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Acta Pharmaceutica Sinica B 2024年第1期14卷 335-349页

作者： Ju Chen Bhaskara Reddy Madina Elham Ahmadi Timur Olegovich Yarovinsky Marie Marthe Krady Eileen Victoria Meehan Isabella China Wang Xiaoyang Ye Elise Pitmon Xian-Yong Ma Bijan Almassian Valerian Nakaar Kepeng Wang Department of Immunology School of MedicineUniversity of Connecticut Health CenterFarmingtonCT 06030USA CaroGen Corporation FarmingtonCT 06030USA The Eighth Clinical Medical College of Guangzhou University of Chinese Medicine Foshan Hospital of Traditional Chinese MedicineFoshan 528000China The Loomis Chaffee School WindsorCT 06095USA

Targeting multiple immune mechanisms may overcome therapy resistance and further improve cancer immunotherapy for ***,we describe the application of virus-like vesicles(VLV)for delivery of three immunomodulators alone and in combination,as a promising approach for cancer *** vectors were designed to deliver single chain interleukin(IL)-12,shorthairpin RNA(shRNA)targeting programmed death ligand 1(PD-L1),and a dominant-negative form of IL-17 receptor A(dn-IL17RA)as a single payload or as a combination *** delivery of the VLV vector expressing IL-12 alone,as well as the trivalent vector(designated CARG-2020)eradicated large established ***,only CARG-2020 prevented tumor recurrence and provided long-term survival benefit to the tumor-bearing mice,indicating a benefit of the combined *** abscopal effects of CARG-2020 on the non-injected contralateral tumors,as well as protection from the tumor cell re-challenge,suggest immune-mediated mechanism of protection and establishment of immunological ***,CARG-2020 potently activates Th1 immune mechanisms and inhibits expression of genes related to T cell exhaustion and cancer-promoting *** ability of CARG-2020 to prevent tumor recurrence and to provide survival benefit makes it a promising candidate for its development for human cancer immunotherapy.

关键词： Virus-like vesicles Oncolytic virus IL-12 IL-17 PD-L1 T cell exhaustion Combination therapy Cancer immunotherapy

Understanding the role of transmembrane 9 superfamily member 1 in bladder cancer pathogenesis

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World Journal of Clinical Oncology 2024年第4期15卷 468-471页

作者： Venkata Krishna Vamsi Gade Budhi Singh Yadav Department of Radiotherapy&Oncology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Department of Radiotherapy&Oncology Regional Cancer CentrePostgraduate Institute of Medical Education and ResearchChandigarh 160012India

In this editorial we comment on the article by Wei et al,published in the recent issue of the World Journal of Clinical *** authors investigated the role of Transmembrane 9 superfamily member 1(TM9SF1)protein in bladder cancer(BC)*** vectors were used to achieve silencing or overexpression of TM9SF1 gene in three BC cell *** cell lines were then subject to cell counting kit 8,wound-healing assay,transwell assay,and flow ***,migration,and invasion of BC cells were increased in cell lines subjected to TM9SF1 ***9SF1 silencing inhibited proliferation,migration and invasion of BC *** authors conclude that TM9SF1 may be an oncogene in bladder cancer pathogenesis.

关键词： Urinary bladder cancer Transmembrane 9 superfamily member 1 gene cell line Lentiviral vectors Wound healing assay Oncogene Proliferation Migration

Truth Discovery with Memory Network

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Tsinghua Science and Technology 2017年第6期22卷 609-618页

作者： Luyang Li Bing Qin Wenjing Ren Ting Liu research center for Social Computing and Information Retrieval Harbin Institute of Technology Harbin 150001 China

Truth discovery aims to resolve conflicts among multiple sources and find the truth. Conventional methods for truth discovery mainly investigate the mutual effect between the reliability of sources and the credibility of statements. These methods use real numbers, which have a lower representation capability than vectors to represent the reliability. In addition, neural networks have not been used for truth discovery. In this work, we propose memory-network-based models to address truth discovery. Our proposed models use feedforward and feedback memory networks to learn the representation of the credibility of statements. Specifically, our models adopt a memory mechanism to learn the reliability of sources for truth prediction. The proposed models use categorical and continuous data during model learning by automatically assigning different weights to the loss function on the basis of their own effects. Experimental results show that our proposed models outperform state-of-the-art methods for truth discovery.

关键词： truth discovery memory networks source reliability

Viewpoint Planning for Freeform Surface Inspection Using Plane Structured Light Scanners

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International Journal of Automation and computing 2016年第1期13卷 42-52页

作者： Qian Wu Wei Zou De Xu research center of Precision Sensing and Control Institute of Automation Chinese Academy of Sciences

This paper proposes an automatic model-based viewpoint planning method, which can achieve high precision and high efficiency for freeform surfaces inspection using plane structured light scanners. The surface model is utilized in stereolithography format, which is widely used as an industrial standard. The proposed method consists of 4 steps： topology reconstruction, mesh refinement, scan direction determination and viewpoint generation. In the first step, the topology structure of the surface model is reconstructed according to a designed data structure, based on which a neighborhood search algorithm is developed. In the second step, big facets in the surface model are segmented into several small ones, which are suitable for viewpoint planning. In the third step, an initial scan region of a viewpoint is grouped by the neighborhood search algorithm combining with total area and normal vector restrictions. Accordingly, the scan direction is determined by the normal vectors of facets in the initial scan region. In the fourth step, the position, the orientation, and the final scan region of the viewpoint are determined by 4 scan constraints, i.e., field of view, working distance range, view angle and overlap. Experimental results verify the effectiveness and advantages of the proposed method.

关键词： Freeform surface 3D measurement structured light scanner stereolithography (STL) viewpoint planning.

The genetic diversity of Spirometra tapeworm isolates from snakes in central China

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The genetic diversity of Spirometra tapeworm isolates from s...

Real-time traffic information extraction based on compressed video with interframe motion vector

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Tengfang Gong Fangfang Xu Fen Li Junlin He Shuyu Chen Wenchao Li Xinrui Xie Yisong Liu Xi Zhang Wei Liu research center for Parasites & vectors College of Veterinary MedicineHunan Agricultural University Department of Parasitology School of Basic Medical SciencesZhengzhou University

Sparganosis,which caused by the larval plerocercoid of Spirometra tapeworms,still poses a potential public health hazard in *** to Sparganosis,the snake is an important source of infection for human ***,sufficient studies on the population genetics of sparganum isolates from snakes have not occurred to *** study collected 67 sparganum isolates from snakes(Zaocys dhumnades,Elaphe carinata),and several definitive hosts(Panthera tigris,Prionailurus bengalensis,Felis silvestris,cat) from15 geographical locations in Hunan Province of *** isolates were identified using a multiplex polymerase chain reaction ***,genetic diversity was also explored based on mitochondrial cytb and cox1 *** result shows that Sparganum isolates collected in the Hunan Province belonged to one genotype of *** genetic diversity analysis disparately revealed no distinct genetic structure among Spirometra *** phylogenetic analysis supported the distinction between Polish and Chinese Spirometra ***,the taxonomy of Spirometra *** ambiguous,consequently more morphological and molecular studies are necessary to clarify the systematics of the genus of Spirometra.

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Journal of Harbin Institute of Technology(New Series) 2003年第3期10卷 284-289页

作者：黄庆明王聪 Joint R&D Lab Graduate School Chinese Academy of Sciences Beijing 100080 China School of Astronautics Harbin Institute of Technology Harbin 150001 Chinaxtraction of traffic information from image or video sequence is a hot research topic in intelligent transportation system and computer vision. A real-time traffic information extraction method based on compressed video with interframe motion vectors for speed density and flow detection has been proposed for extraction of traffic information under fixed camera setting and well-defined environment. The motion vectors are first separated from the compressed video streams and then filtered to eliminate incorrect and noisy vectors using the well-defined environmental knowledge. By applying the projective transform and using the filtered motion vectors speed can be calculated from motion vector statistics density can be estimated using the motion vector occupancy and flow can be detected using the combination of speed and density. The embodiment of a prototype system for sky camera traffic monitoring using the MPEG video has been implemented and experimental results proved the effectiveness of the method proposed.

Extraction of traffic information from image or video sequence is a hot research topic in intelligenttransportation system and computer vision. A real-time traffic information extraction method based on com-pressed video with interframe motion vectors for speed, density and flow detection, has been proposed for ex-traction of traffic information under fixed camera setting and well-defined environment. The motion vectors arefirst separated from the compressed video streams, and then filtered to eliminate incorrect and noisy vectors u-sing the well-defined environmental knowledge. By applying the projective transform and using the filtered mo-tion vectors, speed can be calculated from motion vector statistics, density can be estimated using the motionvector occupancy, and flow can be detected using the combination of speed and density. The embodiment of aprototype system for sky camera traffic monitoring using the MPEG video has been implemented, and experi-mental results proved the effectiveness of the method proposed.

关键词： extraction of traffic information Interframe motion vector compressed video stream

Modularized viromimetic polymer nanoparticle vaccines(VPNVaxs) to elicit durable and effective humoral immune responses

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National Science Review 2024年第03期11卷 200-214页

作者： Zichao Huang Xinyu Zhuang Liping Liu Jiayu Zhao Sheng Ma Xinghui Si Zhenyi Zhu Fan Wu Ningyi Jin Mingyao Tian Wantong Song Xuesi Chen Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Sciences School of Applied Chemistry and Engineering University of Science and Technology of China Changchun Veterinary research Institute Chinese Academy of Agricultural Sciences Jilin Biomedical Polymers Engineering Laboratory State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

Virus-like particle(VLP) vaccines had shown great potential during the COVID-19 pandemic, and was thought to be the next generation of antiviral vaccine technology due to viromimetic structures. However,the time-consuming and complicated processes in establishing a current recombinant-protein–based VLP vaccine has limited its quick launch to the out-bursting pandemic. To simplify and optimize VLP vaccine design, we herein report a kind of viromimetic polymer nanoparticle vaccine(VPNVax), with subunit receptor-binding domain(RBD) proteins conjugated to the surface of polyethylene glycol-b-polylactic acid(PEG-b-PLA) nanoparticles for vaccination against SARS-CoV-2. The preparation of VPNVax based on synthetic polymer particle and chemical post-conjugation makes it possible to rapidly replace the antigens and construct matched vaccines at the emergence of different viruses. Using this modular preparation system, we identified that VPNVax with surface protein coverage of 20%–25% had the best immunostimulatory activity, which could keep high levels of specific antibody titers over 5 months and induce virus neutralizing activity when combined with an aluminum adjuvant. Moreover, the polymer nano-vectors could be armed with more immune-adjuvant functions by loading immunostimulant agents or chemical chirality design. This VPNVax platform provides a novel kind of rapidly producing and efficient vaccine against different variants of SARS-CoV-2 as well as other viral pandemics.

关键词： vaccine polymer nanomaterials SARS-CoV-2

Viral vectors as a novel tool for clinical and neuropsychiatric research applications

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General Psychiatry 2018年第5期31卷 100-108页

作者： Yao Wang Zhiwei Hu Peijun Ju Shan Yin Fujie Wang Oudong Pan Jinghong Chen research and development department OBiO Technology (Shanghai) Corp. Ltd. Marketing department OBiO Technology (Shanghai) Corp. Ltd. Shanghai Mental Health center Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Psychotic Disorders

Background A viral vector is a genetically modified vector produced by genetic engineering. As pathogenic genes in the virus are completely or largely eliminated, it is safe to be widely used in multidisciplinary research fields for expressing genes, such as neuroscience, metabolism, oncology and so on. Neuroscience and psychiatry are the most closely related disciplines in either basic research or clinical research, but the application of viral vectors in neuropsychiatry has not received much attention or not been widely *** This article will focus on the application of viral vectors in basic and clinical neuropsychiatric *** By using viral vectors, scientists can perform neurological labelling, gene expression regulation and physiological manipulation for investigating phenomenon from molecular mechanisms to behaviours. At the same time, to treat mental or neurological disorders, viral vectors can be designed for gene therapy, which alter gene expression levels or repair mutated genes in the brains of *** Viral vectors play an important role in basic research and clinical applications. To further understand brain function and prevent mental and neurological diseases, we hypothesize that viral vectors could be used along with various advanced technologies, such as sequencing and high-throughput expression analysis in the neuroscience research field.

关键词： Methods By using viral vectors Neuroscience and psychiatry