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Clinical outcomes of celiac artery coverage with vs. without revascularization in thoracic endovascular aortic repair

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中华医学杂志(英文版) 2024年第14期137卷 2024 Jun 25页

作者： Xie Xinsheng Zhang Wei Fu Weiguo Zhang Wayne W. Wang Lixin Department of vascular Surgery Xiamen Branch Zhongshan Hospital Fudan University Xiamen Fujian China Department of vascular Surgery Zhongshan Hospital Fudan University Shanghai China Department of Plastic surgery Plastic Surgery Hospital Peking Union Medical College Beijing China Department of Division of vascular and Endovascular Surgery Seattle VA Medical Center University of Washington Seattle WA USA

Integrin binding peptides facilitate growth and interconnected vascular-like network formation of rat primary cortical vascular endothelial cells in vitro

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Neural Regeneration Research 2023年第5期18卷 1052-1056页

作者： Ram Kuwar Xuejun Wen Ning Zhang Dong Sun Department of Anatomy and Neurobiology Medical College of Virginia CampusVirginia Commonwealth UniversityRichmondVAUSA Department of Chemical and Life Science Engineering College of EngineeringVirginia Commonwealth UniversityRichmondVAUSA Department of Biomedical Engineering College of EngineeringVirginia Commonwealth UniversityRichmondVAUSA

Neovascularization and angiogenesis in the brain are important physiological processes for normal brain development and repair/regeneration following insults. Integrins are cell surface adhesion receptors mediating important function of cells such as survival, growth and development during tissue organization, differentiation and organogenesis. In this study, we used an integrin-binding array platform to identify the important types of integrins and their binding peptides that facilitate adhesion, growth, development, and vascular-like network formation of rat primary brain microvascular endothelial cells. Brain microvascular endothelial cells were isolated from rat brain on post-natal day 7. Cells were cultured in a custom-designed integrin array system containing short synthetic peptides binding to 16 types of integrins commonly expressed on cells in vertebrates. After 7 days of culture, the brain microvascular endothelial cells were processed for immunostaining with markers for endothelial cells including von Willibrand factor and platelet endothelial cell adhesion molecule. 5-Bromo-2′-dexoyuridine was added to the culture at 48 hours prior to fixation to assess cell proliferation. Among 16 integrins tested, we found that α5β1, αvβ5 and αvβ8 greatly promoted proliferation of endothelial cells in culture. To investigate the effect of integrin-binding peptides in promoting neovascularization and angiogenesis, the binding peptides to the above three types of integrins were immobilized to our custom-designed hydrogel in three-dimensional(3 D) culture of brain microvascular endothelial cells with the addition of vascular endothelial growth factor. Following a 7-day 3 D culture, the culture was fixed and processed for double labeling of phalloidin with von Willibrand factor or platelet endothelial cell adhesion molecule and assessed under confocal microscopy. In the 3 D culture in hydrogels conjugated with the integrin-binding peptide, brain microvascular endothelial c

关键词： 3D culture angiogenesis brain microvascular endothelial cells hydrogel integrins platelet endothelial cell adhesion molecule(PECAM-1) vascular endothelial growth factor(VEGF) vascularization

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Unlocking antitumor immunity with adenosine receptorblockers

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Cancer Drug Resistance 2023年第4期6卷 748-767页

作者： Victoria A.Remley Joel Linden Todd W.Bauer Julien Dimastromatteo Department of Surgery University of VirginiaCharlottesvilleVA 22903USA Adovate CharlottesvilleVA 22901USA University of Virginia Comprehensive Cancer Center CharlottesvilleVA 22903USA

Tumors survive by creating a tumor microenvironment(TME)that suppresses antitumor *** TME suppresses the immune system by limiting antigen presentation,inhibiting lymphocyte and natural killer(NK)cell activation,and facilitating T cell *** inhibitors like anti-PD-1 and anti-CTLA4 are immunostimulatory antibodies,and their blockade extends the survival of some but not all cancer *** adenosine triphosphate(ATP)is abundant in inflamed tumors,and its metabolite,adenosine(ADO),is a driver of immunosuppression mediated by adenosine A2A receptors(A2AR)and adenosine A2B receptors(A2BR)found on tumor-associated lymphoid and myeloid *** review will focus on adenosine as a key checkpoint inhibitor-like immunosuppressive player in the TME and how reducing adenosine production or blocking A2AR and A2BR enhances antitumor immunity.

关键词： Immunotherapy adenosine adenosine receptors adenosine A2A receptors(A2AR) adenosine A2B receptors(A2BR) tumor cells immune cells tumor microenvironment

Adventures Beyond Amdahl's Law:How Power-Performance Measurement and Modeling at Scale Drive Server and Supercomputer Design

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Journal of Computer Science & Technology 2023年第1期38卷 80-86页

作者： Kirk W.Cameron Computer Science Virginia TechWashington D.C.VA 24061U.S.A.

Amdahl’s Law painted a bleak picture for large-scale *** implication was that parallelism was limited and therefore so was potential *** Amdahl’s contribution was seminal and important,it drove others vested in parallel processing to define more clearly why large-scale systems are critical to our future and how they fundamentally provide opportunities for speedup beyond Amdahl’s *** the early 2000s,much like Amdahl,we predicted dire consequences for large-scale systems due to power *** our early work was often dismissed,the implications were clear to some:power would ultimately limit *** this retrospective,we discuss how power-performance measurement and modeling at scale led to contributions that have driven server and supercomputer design for more than a *** the influence of these techniques is now indisputable,we discuss their connections,limits and additional research directions necessary to continue the performance gains our industry is accustomed to.

关键词： Amdahl’s Law speedup power-aware computing power modeling performance modeling performance prediction power measurement

Effects of contaminant metal ions on precipitation recovery of rare earth elements using oxalic acid

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Journal of Rare Earths 2022年第3期40卷 482-490,I0005页

作者： Wencai Zhang Aaron Noble Bin Ji Qi Li Department of Mining and Minerals Engineering Virginia Polytechnic Institute and State UniversityBlacksburgVA24061USA

Solution equilibrium calculations were performed in this study to understand the impact of contaminant metal ions on the precipitation efficiency of selected rare earth elements(Ce^(3+),Nd^(3+),and Y^(3+))using oxalic acid as a *** metal ions,Al^(3+) and Fe^(3+),are found to considerably affect the precipitation efficiency of *** Al^(3+) and Fe^(3+)concentrations are increased by 1×10^(−4) mol/L,in order to achieve an acceptable cerium recovery of 93%from solutions containing 1×10^(−4) mol/L Ce^(3+),oxalate dosage needs to increase by 1.2×10^(−4) and 1.68×10^(−4) mol/L,*** great impacts on the required oxalate dosage are also observed for Nd^(3+) and Y^(3+),which indicates that oxalic acid consumption and cost will be largely increased when the trivalent metal ions exist in REE-concentrated *** of the divalent metal ions on the oxalate dosage are ***,solution equilibrium calculation results show that the precipitation of Fe^(3+) and Ca^(2+)(e.g.,hematite and Ca(C_(2)O_(4))∙H_(2)O(s))likely occurs during the oxalate precipitation of REEs at relatively high pH(e.g.,pH 2.5),which will reduce rare earth oxalate product *** addition to the metal ions,anionic species,especially SO_(4)^(2-),are also found to negatively affect the precipitation recovery of *** example,when 0.1 mol/L SO_(4)^(2-) occurs in a solution containing 1×10^(−4) mol/L Ce^(3+) and 4×10^(−4) mol/L oxalate,the pH needs to be elevated from 2.0 to 3.3 to achieve the acceptable ***,findings from this study provide guidance for the obtainment of high-purity rare earth products from solutions containing a considerable amount of contaminant metal ions by means of oxalic acid precipitation.

关键词： Rare earth elements Oxalic acid Precipitation Contaminant metal ions

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Predicting the outcome of closed-loop small bowel obstruction by preoperative characteristics

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World Journal of Gastrointestinal Surgery 2022年第6期14卷 556-566页

作者： Masja K Toneman Bente M de Kok Frank M Zijta Stanley Oei Gijs J D van Acker Marinke Westerterp Anne E M van der Pool Department of Surgery Haaglanden Medical CentreThe Hague 2512 VANetherlands Department of Radiology Haaglanden Medical CentreThe Hague 2512 VANetherlands

BACKGROUND Closed-loop small bowel obstruction(CL-SBO)can threaten the viability of the intestine by obstructing a bowel segment at two adjacent *** recognition and surgery are *** To analyze the outcomes of patients who underwent surgery for CL-SBO and to evaluate clinical *** Patients who underwent surgery for suspected CL-BSO on computed tomography(CT)at a single center between 2013 and 2019 were evaluated *** were divided into three groups by perioperative outcome,including viable bowel,reversible ischemia,and irreversible *** and laboratorial variables at presentation were compared and postoperative outcomes were *** Of 148 patients with CL-SBO,28(19%)had a perioperative viable small bowel,86(58%)had reversible ischemia,and 34(23%)had irreversible *** with a higher age had higher risk for perioperative irreversible ischemia[odds ratio(OR):1.03,95%confidence interval(CI):0.99-1.06].Patients with American Society of Anaesthesiologists(ASA)classification≥3 had higher risk of perioperative irreversible ischemia compared to lower ASA classifications(OR:3.76,95%CI:1.31-10.81).Eighty-six patients(58%)did not have elevated C-reactive protein(>10 mg/L),and between-group differences were *** in-hospital stay was significantly longer for patients with irreversible ischemia(median 8 d,P=0.001)than for those with reversible ischemia(median 6 d)or a viable bowel(median 5 d).Postoperative morbidity was significantly higher in patients with perioperative irreversible ischemia(45%,P=0.043)compared with reversible ischemia(20%)and viable bowel(4%).CONCLUSION Older patients or those with higher ASA classification had an increased risk of irreversible ischemia in case of *** irreversible ischemia,postoperative morbidity was increased.

关键词： General surgery Laparoscopy Laparotomy Critical care Intestinal obstruction Morbidity

Robust identification of regulatory variants(eQTLs)using a differential expression framework developed for RNA‑sequencing

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畜牧与生物技术杂志:英文版 2023年第5期14卷 1869-1879页

作者： Mackenzie A.Marrella Fernando H.Biase School of Animal Sciences Virginia Polytechnic Institute and State UniversityBlacksburgVAUSA

Background A gap currently exists between genetic variants and the underlying cell and tissue biology of a trait,and expression quantitative trait loci(eQTL)studies provide important information to help close that ***,two concerns that arise with eQTL analyses using RNA-sequencing data are normalization of data across samples and the data not following a normal *** pipelines have been suggested to address *** instance,the most recent analysis of the human and farm Genotype-Tissue Expression(GTEx)project proposes using trimmed means of M-values(TMM)to normalize the data followed by an inverse normal *** In this study,we reasoned that eQTL analysis could be carried out using the same framework used for dif-ferential gene expression(DGE),which uses a negative binomial model,a statistical test feasible for count *** the GTEx framework,we identified 35 significant eQTLs(Pva model and 39 significant eQTLs(Pvalent to the ANOva and additive model,*** we compared the two approaches,there was no overlap of significant eQTLs between the two *** we defined specific contrasts,we identified trans eQTLs that more closely resembled what we expect from genetic variants showing complete dominance between ***,these were not identified by the GTEx *** Our results show that transforming RNA-sequencing data to fit a normal distribution prior to eQTL analysis is not required when the DGE framework is *** proposed approach detected biologically relevant variants that otherwise would not have been identified due to data transformation to fit a normal distribution.

关键词： Differential gene expression eQTL analysis Gene expression RNA-sequencing Single nucleotide polymorphism

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The Impact of Psychosocial Influences on Chronic Wound Healing

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Open Journal of Medical Psychology 2024年第3期13卷 39-57页

作者： Kelly Frasier Vivian Li Sara Christoforides Kathleen Daly Alexandra Loperfito Karina Stech Milena Dragovic Nuvance Health/vassar Brothers Medical Center Poughkeepsie NY USA Lake Erie College of Osteopathic Medicine New York NY USA Medical College of Georgia Augusta University Augusta GA USA Edward Via College of Osteopathic Medicine Blacksburg VA USA Lewis Katz School of Medicine Temple University Philadelphia PA USA College of Osteopathic Medicine California Health Sciences University Clovis CA USA

This comprehensive review explores the intricate dynamics between psychosocial factors and chronic wound healing processes, specifically focusing on prevalent conditions such as pressure ulcers, diabetic foot ulcers, and venous leg ulcers. By examining the roles of psychiatric conditions, including depression, anxiety, and post-traumatic stress disorder (PTSD), this paper illuminates how these factors intricately influence wound healing dynamics, including mechanisms of pain perception and inflammatory responses. Furthermore, we evaluate the effectiveness of integrated biopsychosocial interventions, which encompass a holistic approach to wound care, thereby enhancing healing outcomes for dermatology patients. Future studies should focus on investigating the specific psychosocial determinants that significantly influence wound healing, exploring novel therapeutic strategies, and implementing personalized interventions to meet the unique needs of each patient. Such endeavors hold promise in advancing the fields of psychodermatology and wound management, fostering a deeper understanding and application of psychosocial considerations in dermatological care.

关键词： Psychosocial Factors Chronic Wound Healing Biopsychosocial Interventions Psychiatric Conditions Dermatology Patients

Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver

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Liver Research 2023年第3期7卷 263-271页

作者： Grayson W.Way Hongkun Lu Xuan Wang Derrick Zhao Carmen Camarena Devanand Sarkar Rebecca K.Martin Huiping Zhou C.Kenneth and Dianne Wright Center for Clinical and Translational Research Virginia Commonwealth UniversityRichmondVAUSA Department of Microbiology and Immunology Virginia Commonwealth University School of MedicineRichmondVAUSA Department of Human and Molecular Genetics Massey Cancer CenterVCU Institute of Molecular Medicine(VIMM)Virginia Commonwealth UniversityRichmondVAUSA Massey Cancer Center Virginia Commonwealth UniversityRichmondVAUSA VCU Institute of Molecular Medicine Virginia Commonwealth UniversityRichmondVAUSA Central Virginia Veterans Healthcare System RichmondVAUSA

The liver plays an important role in both metabolism and *** of the hepatic immune microenvironment is closely associated with various liver *** gain a better understanding of how different types of immune cells contribute to the progression of liver diseases,it is crucial to thoroughly characterize hepatic immune *** direct digestion of liver tissue is a relatively simple method for isolating immune cells,it often induces excessive hepatocyte death,which causes a release of intracellular components that leads to the activation of stress responses and injury in the surrounding *** injury can lead to excessive death in the hepatic immune cells,making isolation and accurate characterization of the immune profile challenging,especially in diseased *** method described here addresses these challenges by utilizing Phosphate buffered saline(PBS)and digestion buffer perfusions to eliminate contaminating blood cells,ensure a pure hepatic immune population,and minimize hepatic immune cell *** ex vivo digestion of the liver enables the isolation of the immune cells from the hepatic tissues and the generation of a single-cell suspension that can be stained for spectral flow *** enhance intracellular cytokine detection and maintain signaling under different physiological and pathological conditions,this protocol uses an in vivo administration of Brefeldin A,a less toxic inhibitor of cytokine *** in vivo administration of Brefeldin A allows for a more accurate representation of the immune cell function and cytokine expression compared to the traditionally used ex vivo Brefeldin A administration.A comprehensive spectral flow cytometry panel,comprising extracellular and intracellular staining,is used for deep immunophenotyping and immune cell effector function *** this protocol is specifically designed for liver digestion of Mdr2 knockout mice(a model for primary sclerosing cholangitis)and f

关键词： metabolism flow utilizing