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Soil Microbial Activities in Beech Forests Under Natural Incubation Conditions as Affected by Global Warming

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Pedosphere 2014年第6期24卷 709-721页

作者： S.LU Q.WANG S.KATAHATA M.NARAMOTO H.MIZUNAGA Graduate School of Science and Technology Shizuoka University Graduate School of Agriculture Shizuoka University

Microbial activity in soil is known to be controlled by various factors. However, the operating mechanisms have not yet been clearly identified, particularly under climate change conditions, although they are crucial for understanding carbon dynamics in terrestrial ecosystems. In this study, a natural incubation experiment was carried out using intact soil cores transferred from high altitude(1 500 m) to low(900 m) altitude to mimic climate change scenarios in a typical cold-temperate mountainous area in Japan. Soil microbial activities, indicated by substrate-induced respiration(SIR) and metabolic quotient(q CO2), together with soil physicalchemical properties(abiotic factors) and soil functional enzyme and microbial properties(biotic factors), were investigated throughout the growing season in 2013. Results of principal component analysis(PCA) indicated that soil microbial biomass carbon(MBC) andβ-glucosidase activity were the most important factors characterizing the responses of soil microbes to global warming. Although there was a statistical difference of 2.82 ℃ between the two altitudes, such variations in soil physical-chemical properties did not show any remarkable effect on soil microbial activities, suggesting that they might indirectly impact carbon dynamics through biotic factors such as soil functional enzymes. It was also found that the biotic factors mainly controlled soil microbial activities at elevated temperature,which might trigger the inner soil dynamics to respond to the changing environment. Future studies should hence take more biotic variables into account for accurately projecting the responses of soil metabolic activities to climate change.

关键词： biotic factors carbon dynamics metabolic quotient microbial biomass soil enzymes soil respiration

The Theaflavin Monomers Inhibit the Cancer Cells Growth in Vitro

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 508-512页

作者： Naoharu WATANABE Department of Applied Biological Chemistry 836 Ohya shizuoka 422-8529 shizuoka university Japan

The inhibition effects of tea theaflavins complex (TFs), theaflavin-3-3’-digallate (TFDG), theaflavin-3’-gallate (TF2B), and an unidentified compound (UC) on the growth of human liver cancer BEL-7402 cells, gastric cancer MKN-28 cells and acute promyelocytic leukemia LH-60 cells were investigated. TFs was obtained through the catalysis of catechins with immobilized polyphenols oxidase. TFDG, TF2B and UC were isolated from TFs with high speed countercurrent chromatography (HSCCC). The results showed that TF2B significantly inhibited the growth of all three kinds of cancer cells, TFs, TFDG and UC had some effect on BEL-7402 and MKN-28, but little activity on LH-60. The inhibition effects of TF2B, TFDG, and UC on BEL-7402 and MKN-28 were stronger than TFs. The relationship coefficients between monomer concentration and its inhibition rate against MKN-28 and BEL-7402 were 0.87 and 0.98 for TF2B, 0.96 and 0.98 for UC, respectively. The IC50 values of TFs, TF2B, and TFDG were 0.18, 0.11, and 0.16 mM on BEL-7402 cells, and 1.11, 0.22, and 0.25 mM on MKN-28 cells respectively.

关键词： theaflavin monomer separation cancer cells inhibition

Measurement of modulation induced by interaction between bubble motion and liquid-phase motion in the decaying turbulence formed by an oscillating-grid

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Particuology 2013年第2期11卷 158-169页

作者： Yasuyuki Nagami Takayuki Saito Graduate School of Engineering Shizuoka University Graduate School of Science and Technology Shizuoka University

In multiphase flows, dynamical gas-liquid interactions are essential for in-depth understanding of their multi-scale phenomena and complicated structures. The purpose of the present study is to clearly extract the modulation in bubble motion and liquid motion induced by bubble-liquid interaction and to discuss the relations between bubble motion and liquid-phase motion. For this particular purpose, the decaying turbulence formed in a cylindrical acrylic pipe （diameter 149 mm, height 600 mm） by using an oscillating- grid was employed. Uniform single bubbles were launched from an in-house bubble launching device into the decaying turbulence. By comparing the bubble motion in the stagnant water with that in the oscillating-grid decaying turbulence, the transition of the 2D bubble motion （i.e., zigzagging motion） to 3D motion was enhanced in the latter. In addition, the initial conditions of the bubble motion that was not influenced by the ambient turbulence were carefully confirmed. In the area where the bubble motion started to translate from 2D motion into 3D motion, the modulation of ambient liquid-phase motion was obtained by PIV/LIF measurement. By combining these results, we quantitatively discussed the modulation of the bubble motion and ambient liquid-phase motion and considered the dominant factor for the enhancement to be the bubble-liquid interaction.

关键词： Multiphase flow Gas-liquid interaction Oscillating-grid decaying turbulence Bubble PIV/LIF LIF/HPTS

Species-dependent effects of the phenolic herbicide ioxynil with potential thyroid hormone disrupting activity: modulation of its cellular uptake and activity by interaction with serum thyroid hormone-binding proteins

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Journal of Environmental Sciences 2012年第5期24卷 949-955页

作者： Sakura Akiyoshi Gobun Sai Kiyoshi Yamauchi Department of Biological Science Faculty of Science Shizuoka University

Ioxynil, a phenolic herbicide, is known to exert thyroid hormone （TH） disrupting activity by interfering with TH-binding to plasma proteins and a step of the cellular TH-signaling pathway in restricted animal species. However, comparative studies are still lacking on the TH disruption. We investigated the interaction of [125I] ioxynil with serum proteins from rainbow trout, bullfrog, chicken, pig, rat, and mouse, using native polyacrylamide gel electrophoresis. Candidate ioxynil-binding proteins, which included lipoproteins, albumin and transthyretin （TTR）, differed among the vertebrates tested. Rainbow trout and bullfrog tadpole serum had the lowest binding activity for ioxynil, whereas the eutherian serum had the highest binding activity. The cellular uptake of, and response to, ioxynil were suppressed by rat serum greater than by tadpole serum. The cellular uptake of [125I]ioxynil competed strongly with phenols with a single ring, but not with THs. Our results suggested that ioxynil interferes with TH homeostasis in plasma and with a step of cellular TH-signaling pathway other than TH-uptake system, in a species-specific manner.

关键词： endocrine disruption ioxynil thyroid hormone serum transthyretin uptake

Surface modification of stratum corneum for drug delivery and skin care by microplasma discharge treatment

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Plasma Science and Technology 2019年第6期21卷 1-8页

作者： J KRISTOF T AOSHIMA M BLAJAN K SHIMIZU Graduate School of Science and Technology Shizuoka University 432-8561 Hamamatsu Japan Department of Electrical and Electronic Engineering Shizuoka University 432-8561 Hamamatsu Japan Organization for Innovation and Social Collaboration Shizuoka University 432-8561 Hamamatsu Japan

Human skin is the largest organ and also the main barrier that prevents foreign substances from entering the *** surface properties of the skin are relevant for transdermal drug delivery and *** micropig skin is used as a substitute for human skin.A microplasma electrode is used for surface modification of the skin epidermal layer of the Yucatan *** dielectric barrier discharge has a thin dielectric as a barrier (~50 μm) and a frequency of 25 *** surface properties of the epidermal layer were characterized by the measurement of the contact angle of the water *** effects of different gases such as air,nitrogen,oxygen,helium or argon were *** change of the contact angle is temporal and it is returned to its initial state after several *** the gases used for plasma ignition,oxygen and argon were the most effective for skin *** distance of the skin from the electrode and the treatment time played a crucial roles in the increasing water contact *** of surface atomic concentration were determined by x-ray photoelectron *** microplasma treatment,the oxygen and nitrogen concentration increased at the skin surface.

关键词： microplasma contact angle skin stratum corneum XPS

A rice mutant displaying a heterochronically elongated internode carries a 100 kb deletion

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Journal of Genetics and Genomics 2011年第3期38卷 123-128页

作者： Mika Hayashi-Tsugane Masahiko Maekawa Qian Qian Hirokazu Kobayashi Shigeru Iida Kazuo Tsugane National Institute for Basic Biology Okazaki 444-8585 Japan Graduate School of Nutritional and Environmental Sciences University of Shizuoka Shizuoka 422-8526 Japan Institute of Plant Science and Resources Okayama University Kurashiki 710-0046 Japan Global Center of Excellence Program University of Shizuoka Shizuoka 422-8526 Japan Graduate School of Pharmaceutical Sciences University of Shizuoka Shizuoka 422-8526 Japan The Graduate university for Advanced Studies (Sokendai) Okazaki 444-8585 Japan

We have isolated a recessive rice mutant, designated as indeterminate growth （ing）, which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion, which corresponds to a 103 kb region in the Nipponbare genome, containing nine annotated genes on chromosome 3. Of these annotated genes, the SLR1 gene encoding a DELLA protein is the only one that is well characterized in its function, and its null mutation, which is caused by a single base deletion in the middle of the intronless SLR1 gene, confers a slender phenotype that bears close resemblance to the ing mutant phenotype. The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene, and the ing mutant appears to be the first characterized mutant having the entire SLR1 sequence deleted. Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene, whose dysfunction must result in a lethal phenotype.

关键词： Heterochronic Elongated intemode Large deletion Map-based cloning Rice genome

Comparison of polycyclic aromatic hydrocarbon pollution in Chinese and Japanese residential air

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Journal of Environmental Sciences 2011年第9期23卷 1512-1517页

作者： Hao Lu Takashi Amagai Takeshi Ohura School of Environmental Science and Engineering Zhejiang Gongshang University Hangzhou Zhejiang 310012 China Institute for Environmental Sciences University of Shizuoka 52-1 Yada Shizuoka 422-8526 Japan

Comparative studies on polycyclic aromatic hydrocarbon （PAH） pollution in residential air of Hangzhou （China） and shizuoka （Japan） were conducted in summer （August,2006） and winter （January,2007）.Total concentrations of 8 PAHs ranged from 7.1 to 320 ng/m 3 and 0.15 to 35 ng/m 3 in residential air of Hangzhou and shizuoka,*** PAH concentrations in smoking houses were higher than that in nonsmoking *** nonsmoking houses,mothball emission and cooking practice were the emission sources of 2and 3-ring PAHs in Hangzhou,*** 2and 3-ring PAHs were from use of insect repellent,kerosene heating and outdoor environment in nonsmoking houses in *** 5and 6-ring PAHs in residential air were mainly from outdoor environment in both *** potencies of PAHs in residential air of Hangzhou were much higher than that in shizuoka.

关键词： polycyclic aromatic hydrocarbons residential air emission source

Appropriate Heart Rate in a Patient with Repaired Tetralogy of Fallot

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Congenital Heart Disease 2022年第6期17卷 647-652页

作者： Aya Miyazaki Hideki Uemura Yasuyo Takeuchi Junya Tomida Yasuo Ono Yoshifumi Fujimoto Norie Mitsushita Akio Ikai Department of Transitional Medicine Division of Congenital Heart DiseaseShizuoka General HospitalShizuokaJapan Congenital Heart Disease Center Nara Medical UniversityKashiharaJapan Department of Cardiology Shizuoka General HospitalShizuokaJapan Department of Clinical Engineering Shizuoka General HospitalShizuokaJapan

Appropriate heart rate in a failing pulmonary ventricle remains unknown, particularly in congenital heart diseasewith unique hemodynamics. A 71-year-old male with repaired tetralogy of Fallot and a pacemaker for a sinusnode dysfunction suffered from heart failure symptoms with preserved left ventricular function. Simply changingthe pacemaker’s lower rate from 60 to 75 bpm, New York Heart Association classification improved from III to II,and hemodynamic parameters drastically improved. We regarded this case as informative. Appropriate heart ratecould be higher in congenital patients with failing right and non-failing left ventricles than in adults with malfunctioningLV.

关键词： Heart rate repaired tetralogy of Fallot failing right ventricle pacemaker lower rate the right-left ventricular interaction

New Early Cretaceous paleomagnetic results from Qilian orogenic belt and its tectonic implications

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Science China Earth Sciences 2002年第6期45卷 565-576页

作者：杨天水林爱明杨振宇孙知明 Institute of Geomechanics Chinese Academy of Geological Sciences Beijing China Department of Earth and Planetary Sciences Shizuoka University Shizuoka Japan

Lower Cretaceous red sedimentary rocks from the depositional basin of East Qilian fold belt have been collected for a paleomagnetic study. Stepwise thermal demagnetization reveals two or three components of magnetization from dark red sandstones. Low-temperature magnetic component is consistent with the present Earth Field direction in geographic coordinates. High-temperature magnetic components are mainly carried by hematite. The mean pole of 19 sites for high-temperature magnetic components after tilt-correction is λ=62.2°N, φ=193.4°E, A95=3.2°, and it passes fold tests at 99% confidence level and reversal tests at 95% confidence level. The paleopole is insignificantly different from that of Halim et al. (1998) from the same sampling area at the 95% confidence level. Compared with paleomagnetic results for North China, South China, and Eurasia, our results suggest that no significant relative latitudinal displacement has taken place between Lanzhou region and these blocks since Cretaceous time. Remarkably, the pole of Lanzhou shows a 20° clockwise rotation with respect to those of North China, South China, and Eurasia. Geological information indicates that the crustal shortening in the western part of Qilian is greater than that in eastern part. In this case, the clockwise rotation of sampling area was related to India/Eurasia collision, and this collision resulted in a left-lateral strike-slip motion of the Altun fault in north Tibetan Plateau after the Cretaceous.

关键词： Qilian Mountain, paleomagnetism, Early Cretaceous, Hekou group.