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Effects of Moringa oleifera aqueous extract on the growth performance,blood characteristics,and histological features of gills and livers in Nile tilapia

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Aquaculture and Fisheries 2024年第1期9卷 85-92页

作者： Mahamoud A.Emam Ramy M.Shourbela Waleed N.El-Hawarry Seham Y.Abo-Kora Fatma Abdel-Monem Gad Ashraf M.Abd El-latif Mahmoud A.O.Dawood histology department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt department of Animal Husbandry and Animal Wealth Development Faculty of Veterinary MedicineAlexandria UniversityAlexandria22758Egypt Pharmacology department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Clinical Pathology department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt department of Aquatic Animal Diseases and Management Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Animal Production department Faculty of AgricultureKafrelsheikh UniversityKafr El-Sheikh33511Egypt The Center for Applied Research on the Environment and Sustainability The American University in CairoCairo11835Egypt

Moringa oleifera is well known as a highly nutritious plant and a water purifier in fish *** fish has many impressive qualities making them very suitable for ***,this study aimed to evaluate the effect of supplementation of Moringa oleifera aqueous extract(MOAE)at varying concentrations(0,100,200,and 400 mg/kg diet for 90 days)on growth performance,hematological,and some biochemical parameters of Nile ***,histological changes and immunohistochemical expression of caspase 3 in gills and livers were *** enhancements(P<0.05)in the final weight,weight gain,specific growth rate,red blood cells,hemoglobin,packed cell volume,white blood cells,and total serum protein were observed in fish fed 200 mg MOAE/kg diet compared with other ***,a significant reduction(P<0.05)of liver and kidney function tests and non-significant changes in lipid profile were *** the other hand,fish fed 400 mg MOAE/kg diet revealed disturbance in the corresponding *** histological degenerative changes were detected in the gills and liver of fish fed 400 mg MOAE/kg *** comparison,fish fed 200 mg MOAE/kg diet showed the best histo-architectures of these organs compared with other *** immune expressions of caspase 3 were determined where gills and liver of fish fed 400 mg MOAE/kg diet revealed extensive number of positive caspase 3 cells indicating severe apoptotic ***,a dietary MOAE at 200 mg/kg diet is recommended to improve the growth performance,hematological parameters,liver and kidney functions in Nile ***,caution must be considered when using MOAE at a concentration over 200 mg/kg diet.

关键词： Growth performance Liver Gills Moringa oleifera Nile tilapia

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Experimental models of high-risk bowel anastomosis in rats:A systematic review

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World Journal of Experimental medicine 2024年第2期14卷 114-131页

作者： Georgios Ntampakis Manousos-Georgios Pramateftakis Elissavet Anestiadou Stefanos Bitsianis Orestis Ioannidis Chryssa Bekiari George Koliakos Maria Karakota Anastasia Tsakona Angeliki Cheva Stamatios Angelopoulos Fourth department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece Laboratory of Anatomy and histology School of Veterinary MedicineFaculty of Health SciencesAristotle University of ThessalonikiThessaloniki 54124Greece Experimental and Research Center Papageorgiou General Hospital of ThessalonikiThessaloniki 56403Greece Laboratory of Biochemistry Faculty of MedicineAristotle University of ThessalonikiThessaloniki 54124Greece department of Pathology Faculty of MedicineAristotle University of ThessalonikiThessaloniki 54124Greece

BACKGROUND Anastomotic leaks remain one of the most dreaded complications in gastrointestinal surgery causing significant morbidity,that negatively affect the patients’quality of *** studies play an important role in understanding the pathophysiological background of anastomotic healing and there are still many fields that require further *** drawn from these studies can lead to interventions or techniques that can reduce the risk of anastomotic leak in patients with high-risk *** the advances in experimental protocols and techniques,designing a high-quality study is still challenging for the investigators as there is a plethora of different models *** To review current state of the art for experimental protocols in high-risk anastomosis in *** This systematic review was performed according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses *** identify eligible studies,a comprehensive literature search was performed in the electronic databases PubMed(MEDLINE)and Scopus,covering the period from conception until 18 October *** From our search strategy 102 studies were included and were categorized based on the mechanism used to create a high-risk *** of assessing anastomotic healing were extracted and were individually *** Anastomotic healing studies have evolved over the last decades,but the findings are yet to be translated into human *** is a need for high-quality,well-designed studies that will help to the better understanding of the pathophysiology of anastomotic healing and the effects of various interventions.

关键词： High-risk anastomosis Rats Experimental models Bowel Colon Anastomotic leak Colon resection Inflammatory bowel disease Intra-abdominal sepsis Bursting pressure

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Dysprosium oxide,alumina,and graphene oxide reinforced polycaprolactone thin films:Thermal stability,morphology,and cell viability

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Journal of Rare Earths 2024年第7期42卷 1337-1343,I0005页

作者： Mohamed Tharwat Elabbasy Mohamed R.Atteya Mai A.Samak Mousa Abdelrahman Tahani AlMahmoud M.A.El-Morsy A.A.Menazea M.F.H.Abd El-Kader department of Public Health College of Public Health and Health InformaticsUniversity of Ha'ilHa'il2440Saudi Arabia Food Control department Faculty of Veterinary MedicineZagazig UniversityZagazig44519Egypt department of Physical Therapy College of Applied Medical SciencesUniversity of Ha'ilHa'ilSaudi Arabia department of histology and Cell Biology Faculty of MedicineZagazig UniversityZagazig44519Egypt College of Nursing University of Ha'ilHa'ilSaudi Arabia College of Science and Humanities in Al-Kharj Physics DepartmentPlasma Technology and Material Science UnitPrince Sattam Bin Abdulaziz UniversityAl-kharj11942Saudi Arabia Physics department Faculty of ScienceUniversity of DamiettaNew Damietta34517Egypt Spectroscopy department Physics Research InstituteNational Research CentreDokki12622GizaEgypt Biophysics department Faculty of ScienceCairo UniversityGizaEgypt

Developing a biomaterial for wound healing applications is still a ***,dysprosium oxide(Dy_(2)O_(3)),aluminum oxide(Al_(2)O_(3)),and graphene oxide(GO)were embedded in cast films based on polycaprolactone(PCL)to be examined for wound dressing *** techniques were used to characterize the fabricated films including X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),Raman,and scanning electron microscopy(SEM),besides their biological *** thermogravimetric analysis(TGA)exhibits high thermal stability as the scaffold weight decreases slightly to 98.6%after raising the temperature from room temperature to 280℃.The cell viability was investigated and it is shown that the viable cells grow up to approximately 93%at 75μg/***,the cell attachment shows excellent behavior as the cells attach on most of the surface of the modified PCL which shows high biocompatibility.

关键词： Wound healing Polycaprolactone Dy_(2)O_(3) Al_(2)O_(3) Biocompatible Rare earths

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Melatonin alleviates oxidative stress,inflammation,apoptosis,and DNA damage in acrylamide-induced nephrotoxicity in rats

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Asian Pacific Journal of Tropical Biomedicine 2023年第3期13卷 121-130页

作者： Fatmaİbaokurgil Hakan Aydin Serkan Yildirim Emin Sengul department of Physiology Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey department of Virology Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey department of Pathology Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey

Objective:To investigate the effects of melatonin on renal inflammation,oxidative stress,apoptosis,as well as DNA and tissue damage in acrylamide-induced nephrotoxicity in ***:Fifty male rats were randomly divided into five *** control group received distilled water by gastric lavage for 11days and the acrylamide group was administered acrylamide(50 mg/kg,i.g.)for 11 *** MEL10+ACR and MEL20+ACR groups received intraperitoneal melatonin 10 and 20 mg/kg,respectively,for 11 days,and acrylamide(50 mg/kg,i.g.)was administered 1h after melatonin *** MEL20 group was injected with melatonin(20 mg/kg)for 11 *** function tests were performed and biochemical and inflammatory parameters were *** addition,histopathological,immunohistochemical,and immunofluorescence examinations were carried ***:Melatonin significantly abated acrylamide-induced rise in serum urea and creatinine *** caused oxidative stress,inflammation,apoptosis,as well as DNA and tissue damage in the *** treatment alleviated acrylamide-induced renal damage by exhibiting antioxidant,anti-inflammatory,and antiapoptotic ***,melatonin significantly ameliorated acrylamide-caused histopathological changes in kidney ***:Melatonin attenuates acrylamide-induced renal oxidative stress,inflammation,apoptosis,and DNA damage in rats.

关键词： Acrylamide Apoptosis Inflammation Melatonin Nephrotoxicity Oxidative stress Rat

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Naringin attenuates oxidative stress,inflammation,apoptosis,and oxidative DNA damage in acrylamide-induced nephrotoxicity in rats

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Asian Pacific Journal of Tropical Biomedicine 2022年第5期12卷 223-232页

作者： Volkan Gelen Serkan Yıldırım EminŞengül AliÇınar FikretÇelebi Merve Küçükkalem Melahat Gök department of Physiology Faculty of Veterinary MedicineKafkas UniversityKarsTurkey department of Pathology Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey department of Physiology Faculty of Veterinary MedicineAtatürk UniversityErzurumTurkey

Objective:To explore the possible effects of naringin on acrylamide-induced nephrotoxicity in ***:Sprague-Dawley rats weighing 200-250 g were randomly divided into five *** control group was given intragastric(i.g.)saline(1 mL)for 10 *** acrylamide group was given *** in saline(38.27 mg/kg titrated to 1 mL)for 10 *** treatment groups were administered with naringin in saline(50 and 100 mg/kg,respectively)for 10 d and given ***(38.27 mg/kg)1 h after naringin *** naringin group was given ***(100 mg/kg)alone for 10 *** day 11,intracardiac blood samples were obtained from the rats when they were under anesthesia,after which they were *** and creatinine concentrations of blood serum samples were analyzed with an ***-linked immunosorbent assay was used to quantify malondialdehyde,superoxide dismutase,glutathione,glutathione peroxidase,catalase,tumor necrosis factor-α,nuclear factor-κB,interleukin(IL)-33,IL-6,IL-1β,cyclooxygenase-2,kidney injury molecule-1,mitogen-activated protein kinase-1,and caspase-3 in kidney *** tissues were also evaluated by histopathological and immunohistochemical examinations for 8-OHdG and ***:Naringin attenuated acrylamide-induced nephrotoxicity by significantly decreasing serum urea and creatinine *** increased superoxide dismutase,glutathione,glutathione peroxidase,and catalase activities and decreased malondialdehyde levels in kidney *** addition,naringin reduced the levels of inflammatory and apoptotic parameters in kidney *** histopathological assay showed that acrylamide caused histopathological changes and DNA damage,which were ameliorated by ***:Naringin attenuated inflammation,apoptosis,oxidative stress,and oxidative DNA damage in acrylamide-induced nephrotoxicity in rats.

关键词： Acrylamide Nephrotoxicity Apoptosis Inflammation Naringin Oxidative stress Oxidative DNA damage

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The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 647-653页

作者： Volkan Gelen Emin Sengül Semin Gedikli Gozde Atila Hamit Uslu Mustafa Makav department of Physiology Veterinary FacultyKafkas UniversityKarsTurkey department of Physiology Veterinary FacultyAtatürk UniversityErzurumTurkey department of histology-Embryology Veterinary FacultyAtatürk UniversityErzurumTurkey department of Health Care Services Atatürk Health Vocational SchoolKafkas UniversityKarsTurkey

Objective: To investigate the effects of quercetin(Q) and rutin on 5-fluorouracil(5-FU)-induced ***: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU(50 mg/kg) in the 18th day. The group rutin 100 was rutin(100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU(50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin(100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized ***: The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU ***: In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity.

关键词： Rat 5-Fluorouracil Quercetin Rutin Hepatotoxicity

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In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft

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Journal of Zhejiang university-Science B(Biomedicine & Biotechnology) 2013年第11期14卷 993-1003页

作者： D. Burcu HAZER Ercan BAL Gülay NURLU Kemal BENLI Serdar BALCI Feral OZTrk Baki HAZER department of Neurosurgery Faculty of MedicineMugla Sitki Kocman University atatürk Research and Medical Center Neurosurgery ClinicMinistry of Health of the Republic of Turkey department of Neurology Faculty of MedicineSchool of MedicineHacettepe University department of Neurosurgery Faculty of MedicineSchool of MedicineHacettepe University atatürk Research and Medical Center Department of PathologyYilditim Beyazit University department of histology and Embryology Faculty of MedicineMugla Sitki Kocman University department of Chemistry Bülent Ecevit University

Objective:This study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube,poly-3-hydroxyoctanoate(PHO)in nerve gap ***:Forty Wistar Albino male rats were randomized into two groups:autologous nerve gap repair group and PHO tube repair *** each group,a 10-mm right sciatic nerve defect was created and reconstructed *** was studied by sciatic function index(SFI),electromyography,and immunohistochemical studies on Days 7,21,45 and 60 of *** was analyzed by the capsule formation around the *** was analyzed by the molecular weight loss in ***:Electrophysiological and histomorphometric assessments demonstrated neuroregeneration in both groups over *** the experimental group,a straight alignment of the Schwann cells parallel to the axons was ***,autologous nerve graft seems to have a superior neuroregeneration compared to PHO *** biodegradation was observed in PHO conduit at the end of 60 ***:Although neuroregeneration is detected in PHO grafts with minor degradation in 60 d,autologous nerve graft is found to be superior in axonal regeneration compared to PHO nerve tube *** conduits were found to create minor inflammatory reaction in vivo,resulting in good soft tissue response.

关键词： Axonal spurting Biodegradable polymer Neuroregeneration Nerve grafting

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Nutrient restriction-activated Fra-2 promotes tumor progression via IGF1R in miR-15a downmodulated pancreatic ductal adenocarcinoma

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1132-1146页

作者： Gian Luca Rampioni Vinciguerra Marina Capece Luca Reggiani Bonetti Giovanni Nigita Federica Calore Sydney Rentsch Paolo Magistri Roberto Ballarin Fabrizio Di Benedetto Rosario Distefano Roberto Cirombella Andrea Vecchione Barbara Belletti Gustavo Baldassarre Francesca Lovat Carlo M.Croce department of Cancer Biology and Genetics and Comprehensive Cancer Center The Ohio State UniversityColumbus 43210 OHUSA department of Clinical and Molecular medicine Faculty of Medicine and PsychologySant’Andrea HospitalUniversity of Rome“Sapienza”Rome 00189Italy department of Diagnostic Clinic and Public Health MedicineUniversity of Modena and Reggio EmiliaModena 41100Italy Hepato-pancreato-biliary Surgery and Liver Transplantation Unit University of Modena and Reggio EmiliaModena 41100Italy Division of Molecular Oncology Centro di Riferimento Oncologico di Aviano(CRO)Istituto di Ricovero e Cura a Carattere Scientifico(IRCCS)National Cancer InstituteAviano 33081Italy

Pancreatic ductal adenocarcinoma(PDAC)is a lethal disease,characterized by an intense desmoplastic reaction that compresses blood vessels and limits nutrient *** aggressiveness largely relies on its extraordinary capability to thrive and progress in a challenging tumor *** of the onco-suppressor miR-15a has been extensively documented in ***,we identified the transcription factor Fos-related antigen-2(Fra-2)as a miR-15a target mediating the adaptive mechanism of PDAC to nutrient *** report that the IGF1 signaling pathway was enhanced in nutrient deprived PDAC cells and that Fra-2 and IGF1R were significantly overexpressed in miR-15a downmodulated PDAC ***,we discovered that miR-15a repressed IGF1R expression via Fra-2 *** miR-15a-low context,IGF1R hyperactivated mTOR,modulated the autophagic flux and sustained PDAC growth in nutrient *** a genetic mouse model,Mir15aKO PDAC showed Fra-2 and Igf1r upregulation and mTOR activation in response to diet ***,nutrient restriction improved the efficacy of IGF1R inhibition in a Fra-2 dependent ***,our results point to a crucial role of Fra-2 in the cellular stress response due to nutrient restriction typical of pancreatic cancer and support IGF1R as a promising and vulnerable target in miR-15a downmodulated PDAC.

关键词： IGF1R Fra miR

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Comparison of pre-treatment and post-treatment use of selenium in retinal ischemia reperfusion injury

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 263-268页

作者： Alper Yazici Hasan Aksit Esin Sogutlu Sari Arzu Yay Haydar Ali Erken Dilek Aksit Harun Cakmak Kamil Seyrek Sitki Samet Ermis department of Ophthalmology Faculty of MedicineBalikesir University department of Biochemistry Faculty of VeterinaryBalikesir University department of histology and Embryology Faculty of Medicine Erciyes University department of Physiology Faculty of Medicine Balikesir University department of Pharmacology Faculty of VeterinaryBalikesir University department of Ophthalmology Faculty of Medicine Adnan Menderes University department of Biochemistry Faculty of Medicine Balikesir University

AIM: To investigate the effects of selenium in rat retinal ischemia reperfusion(IR) model and compare pretreatment and post-treatment ***: Selenium pre-treatment group(n =8) was treated with intraperitoneal(i.p.) selenium 0.5 mg/kg for7 d and terminated 24 h after the IR injury. Selenium posttreatment group( n = 8) was treated with i. p. selenium0.5 mg/kg for 7d after the IR injury with termination at the end of the 7d period. Sham group(n =8) received *** injections identical to the selenium volume for 7d with termination 24 h after the IR injury. Control group(n =8) received no intervention. Main outcome measures were retina superoxide dismutase(SOD), glutathione(GSH),total antioxidant status(TAS), malondialdehyde(MDA),DNA fragmentation levels, and immunohistological apoptosis ***: Compared to the Sham group, selenium pre-treatment had a statistical difference in all parameters except SOD. Post-treatment selenium also resulted in statistical differences in all parameters except the MDA levels. When comparing selenium groups, the pre-treatment selenium group had a statistically higher success in reduction of markers of cell damage such as MDA and DNA fragmentation. In contrast, the post-selenium treatment group had resulted in statisticallyhigher levels of GSH. Histologically both selenium groups succeeded to limit retinal thickening and apoptosis. Pre-treatment use was statistically more successful in decreasing apoptosis in ganglion cell layer compared to post-treatment ***: Selenium was successful in retinal protection in IR injuries. Pre-treatment efficacy was superior in terms of prevention of tissue damage and apoptosis.

关键词： retina selenium ischemia-reperfusion apoptosis pre-treatment post-treatment oxidative stress

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Rescue effects of aqueous seed extracts of Foeniculum vulgare and Carum carvi against cadmium-induced hepatic, renal and gonadal damage in female albino rats

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Asian Pacific Journal of Tropical medicine 2017年第12期10卷 1123-1133页

作者： Ahmed Abdel-Wahab Abdel-Razik Hashem Abdel-Razik Rabie Lotfy Abdel Aziz Physiology department Faculty of Veterinary MedicineMinia University61519 El-MiniaEgypt histology department Faculty of Veterinary MedicineBeni-Suef University62512 Beni-SuefEgypt department of Theriogenology Faculty of Veterinary MedicineBeni-Suef University62512 Beni-SuefEgypt

Objective: To investigate the protective effects of aqueous seed extracts of fennel"Foeniculum vulgare"(FVE) and caraway "Carum carvi"(CCE) on liver, kidney and reproductive organs in female rats against cadmium chloride(CC) ***: A total of 36 adult female rats were divided into six groups, six in each *** group(fed normal diet), CC-treated group(50 mg CC/kg diet), CCE-treated group(150 mg CCE/kg diet), CCE + CC group, FVE(150 mg/kg diet) and FVE + CC. One month later, all rats were sacrificed and all samples were collected at proestrus ***: The toxic effects of CC were confirmed biochemically by significant increase of serum concentration of liver enzymes(P rked pathological changes in renal, hepatic, ovarian and uterine tissues. All toxic findings observed in liver and kidney with CC treatment were found to be ameliorated markedly after co-treatments with FVE or CCE. Furthermore, co-treatment of FVE with CC improved significantly all studied reproductive parameters(P < 0.01).Conclusions: Both FVE and CCE could be used as efficient treatments for liver and kidney against CC intoxication. Moreover, FVE could be utilized as a potent treatment to protect and improve female fertility from cadmium intoxication.

关键词： Cadmium Carum carvi Foeniculum vulgare Liver Kidney Fertility

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