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Advances in Climate Change Research 2019年第3期10卷 181-191页

作者： Kayaba HARO Issoufou OUARMA Bernard NANA Antoine BERE Guy Christian TUBREOUMYA Sie Zacharie KAM Patricia LAVILLE Benjamin LOUBET Jean KOULIDIATI Laboratoire de Physique et de Chimie de I'Environnement Universite Joseph Kl-ZERBOOuagadougou03 BP 7021 Ouagadougou 03Burkina Faso INRA AgroParisTech VMR ECOSYSThiverval-GrignonF-78850France Institut des Sciences Ouagadougou01 BP 1757 Ouagadougou 01Burkina Faso

Methane (CH4) and carbon dioxide (C02) surface emissions from Polesgo's landfill (ouagadougou, Burkina Faso) were measured using the static chamber technique in 2017 and 2018. The Polesgo's landfill was composed of four zones: Phase I, II, Phase III, and SP. The surface of Phase I was fully covered and its conditions are better for surface emission measurements. As results concerning the Phase I zone, the geospatial means flux rates of CH4 (657 mg m-2 h l in 2017 and 1210 mg m 2 h_, in 2018, respectively) are measured higher than the tolerable value reported in literature. The emitted CH4 or C 02 have permitted to locate higher surface emissions which are related to the cover state. The calculated gas collection efficiency (27.4% in 2017 and 23.0% in 2018) is low compared to those reported for landfills integrating landfill gas (LFG) extraction system. The carbon footprint calculations (24,966 tC02-eq 2017 and 40,025 tC02-eq in 2018, respectively) shown that Polesgo's landfill is a significant source of greenhouse gases (GHG) and its important potential for organic recovery can contribute to reduce the carbon footprint.

关键词： Municipal solid waste landfilling CH4 C 02 Collection efficiency Static chamber method Carbon footprint

Pharmacological properties and related constituents of stem bark of Pterocarpus erinaceus Poir.(Fabaceae)

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Asian Pacific Journal of Tropical Medicine 2012年第1期5卷 46-51页

作者： Ouedraogo Noufou Sawadogo Richard Wamtinga Tibiri Andre Bayet Christine Lompo Marius Hay A Emmanuelle Koudou Jean Dijoux Marie-Genevieve Guissou Innocent Pierre departement de medecine-pharmacopee traditionnelle/pharmacie(IRSS/CNRST) 03 BP 7192 ouagadougou 03 Burkina Faso Laboratoire de pharmacologie et toxicologie UFR/SDSUniversite de Ouagadougou 03 BP 7021 Ouagadougou 03Burkina Faso universite de Lyon F-69622LyonFrance universite Lyon I Villeurbanne CNRS UMR 5557Ecologie MicrobienneISPB69373 LyonFrance Laboratoire de chimie des substances naturelles Faculte des SciencesUniversite de Bangui.BP908Bangui.Republique Centrafricaine departement de medecine-pharmacopee traditionnelle/pharmacie(IRSS/CNRST) 03 BP 7192 ouagadougou 03 Burkina Faso Laboratoire de pharmacologie et toxicologieUFR/SDSUniversite de Ouagadougou 03 BP 7021 Ouagadougou 03Burkina Faso

Objective:To sereen methanol and dichloromethane extracts of stem bark of Pterocarpus erinaceus for anti-inflammatory,*** vitro antioxidant activities and phytochemical ***:Anti-inflammatory activity was detcrmined by using carrageenan inducededema of mice paw and croton oil-induced edema of mice ear:analgesic effect was evaluated using acetic acid-induced *** screening of extracts was performed by thin layer *** chromatographic fractionation led to the isolation of main active components as *** and *** structures were established hy TLC and nuclear magnetic resonance ***:Both methanol and dichloromethane extracts, *** and epicatechin showed a significant anti—inflammatory effect using croton oil induced—ear ***,the action of dichloroinethane extract was more important. At the doses of 100 and 200 mg/*** methanol extract was able to reduce the carrageeuan induced-hind paw edema,while al the doses of 100.200 and 400 mg/*** showed an important analgesic effect against writhing induced by acetic acid injection of 38.8%.68.0%and 74.3%, *** properties of methanol extract and its dichloromethane and ethyl acetate fractions were assessed by using the 1,1—diphenyl—2—picrylhydrazyl *** methanol extract showed the stronger radical scavenging activity than dichloromethane and ethyl acetate fractions,with an antiradical power of 5.3.5 and 2 *** main components isolated from these extracts as friedelin,lupeol and epicathechin were responsible of these ***:The results suggest that the stem hark extracts of Pterocarpus erinaceus possessed important *** activities and strong antioxidant properties, therefore,they could he used as natural potential ingredients for pharma ceutical industry.

关键词： Pterocarpus erinaceus Phytochemical Anti-inflammatory Analgesic Antioxidant

Identification of pests and assessment of their damage on Carapa procera and Lophira lanceolata in Burkina Faso,West Africa

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Journal of Forestry Research 2017年第3期28卷 585-591页

作者： Baslayi Tindano Olivier Gnankine Amade Ouedraogo Mamadou Traore Jφrgen Axelsen Anne Mette Lykke Unite de Formation et de Recherche en Sciences de la vie et de la Terre Laboratoire d'Entomologie Fondamentale et AppliqueeUniversite de Ouagadougou03 BP 7021 OuagadougouBurkina Faso Unite de Formation et de Recherche en Sciences de la vie etde la Terre Laboratoire de Biologie et Ecologie VegetalesUniversite de Ouagadougou03 BP 7021 OuagadougouBurkina Faso INERA Institut de l'Environnement et de Recherches Agricoles01 BP 476 Ouagadougou 01Burkina Faso department of Bioscience Aarhus UniversityAarhusDenmark

Nontimber forest products are a source of income for women in rural African communities. However, these products are frequently damaged by insect pests. The present study investigates the diversity and damage rates of insect pests that attack Carapa procera seeds and Lophira lanceolata fruits. The experiment was set up in western Burkina Faso and, for C. carapa, consisted of pests collected from seeds that had fallen to the ground and from stockpiled seeds. For L. lanceolata, pests were collected from fruits on the trees, and on the ground. The collected samples were sent to the laboratory to estimate the proportion of damaged seeds/fruits and rear the insects. The results showed that Ephestia spp., Tribolium castaneum, Oryzeaphilus spp., and Tenebroides mauritanicus were the pests of Carapa procera seeds and Lophira lanceolata fruits. Ephestia spp. was recorded as the main pest of both C. proeera and L. lanceolata, whereas T. castaneum was only detected from seeds of L. lanceolata. For C. procera, the stocks were the most infested （29 %） by Ephestia spp. The infestation rate of fruits of L. lanceolata by Ephestia spp, on trees （31.42 ± 3.75 %） was less than the rate of fruits by T. castaneum on the ground （44.00 ± 3.5 %）. The different body sizes of Ephestia spp. may indicate the occurrence of two putative species, one from C. procera and another one from L. lanceolata. This work provides important information that could contribute to the setting up of a local-scale sustainable management framework for oil tree pests in Burkina Faso and surrounding countries.

关键词： Insect diversity Carapa procera Lophiralanceolata Ephestia spp. Pest management

Enteric parasites prevalence at Saint Camille Medical Centre in ouagadougou,Burkina Faso

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Asian Pacific Journal of Tropical Medicine 2011年第5期4卷 401-403页

作者： Simplice Damintoti Karou Drissa Sanou Djeneba Ouermi Salvatore Pignatelli Virginio Pietra Remy Moret Jean Baptiste Nikiema Jacques Simpore Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA) OuagadougouBurkina Faso Ecole Superieure des Techniques Biologiques et Alimentaires(RSTBA-UL) Universite de LomeTogo Unite de Formation et de Recherche en Sciences de la Vie et de la Terre(UFR/SVT) Universite de OuagadougouBurkina Faso Centre medical saint Camille de ouagadougou Burkina Faso

Objective:To assess the prevalence of parasitic infections among patients attending Saint Camille Medical Centre and to estimate co-parasitic infections ***:From January to december 2009,stool samples were collected from 11 728 persons,aged from five months to 72 years and suffering from *** macroscopic description,the stools were examined by light microscopy to search for the presence of ***:From the 11 728 analyzed stools,6 154(52.47%) were infected with at least one *** frequently encountered were:Giardia intestinalis(43.47%),Entamoeba histolytica/Entamoeba dispar(30.74%) and Trichomonas irUestinalis(21.72%),while Hymenolepis nana(2.25%) was the most common ***-infections occurred in 22.34%*** the multi-infected samples,dual and triple infections accounted for 71.18%and 20.00%,*** irUestinalis for protozoan and Hymenolepis nana for helminths were the most implicated ***: This study confirms that intestinal parasites are still a public health problem in Burkina *** reduce the incidence of parasitic infections,it is necessary to promote the education of people so that they practice the rules of individual and collective hygiene.

关键词： Gastroenteritis Giardia intestinalis Hymenolepis nana Helminths Burkina Faso

Seroprevalence of toxoplasmosis and rubella in pregnant women attending antenatal private clinic at ouagadougou,Burkina Faso

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Asian Pacific Journal of Tropical Medicine 2012年第10期5卷 810-813页

作者： Laure Stella Ghoma Linguissi Bolni Marius Nagalo Cyrille Bisseye Thrse S Kagon Mahamoudou Sanou Issoufou Tao Victoire Benao Jacques Simpor Bibiane Kon Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA/LABIOGENE) UFR/SVTUniversite de OuagadougouBurkina Faso departement de Biologie Universite des Sciences et Techniques de MasukuFrancevilleGabon Centre MURAZ Bobo-DioulassoBurkina Faso Centre National de Transfusion Sanguine(CNTS) Ouagadougou.Burkina Faso Clinique Moussa Kone OuagadougouBurkina Faso Centre Medical Saint Camille OuagadougouBurkina Faso

Objective:To evaluate the prevalence of toxoplasmosis and rubella among pregnant women at ouagadougou in Burkina ***:All patient sera were tested for rubella and toxoplasmosis anti-IgG using commercial ELISA kits(PlateliaTMRubella IgG and Platelia? Toxo IgG).The presence of anti-rubella and anti-toxoplasmosis tgM in serum samples was tested using commercial ELISA kits Platelia Rubella TgM and Platelia Toxo ***:Among all the pregnant women tested for toxoplasmosis and rubella,their prevalence were 20.3% and 77.0%,*** women in the age group of 18-25 years showed the highest frequency of anti-toxoplasmosis（34.5%） and anti-rubella IgG（84.6%）.The prevalence of anti-toxoplasma and anti-rubella IgG decreased between 2006 and 2008 from 32.7%to 12.1%and 84.6% to 65.0%,*** was no significant association between age and the mean titer of anti-toxoplasmosis IgG among pregnant ***:The diagnosis of toxoplasmosis and rubella is necessary in pregnant women in Burkina Faso because of the low immunization coverage rate of rubella and the high level of exposure to these two infections which can be harmful to the newborn if contracted by women before the third trimester of pregnancy.

关键词： IgG Toxoplasma gondii Rubella Seroprevalence Burkina Faso

Antimicrobial,antiplasmodial,haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants

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Asian Pacific Journal of Tropical Medicine 2011年第10期4卷 808-813页

作者： Simplice D Karou Tchadjobo Tchacondo Lassina Ouattara Kokou Anani Aly Savadogo Amegnona Agbonon Mossaclok Ben Attaia Comlan de Souza Mohsen Sakly Jacques Simpore Centre de Recherche et de Formation sur les Plantes Medicinales(CERFOPLAM) Universite de LomeTogo Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA/LABIOGENE) OuagadougouBurkina Faso Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles(CRSBAN)Universite de OuagadougouBurkina Faso Laboratoire de Biosurveillance de l'Environnement Facultes des Sciences de BizerteUniversite 7 Novembre a CarthageTunisTunisie

Objective:To investigate the antioxidant,antimicrobial,antiplasmodial,acute toxicity and haemolytic activities of methanolic extracts of three *** analysis to determine the phenolic contents was also carried ***:The 2,2-diphenyl-1- picryl-hydrazyl（DPPH） free radical scavenging,NCCLS broth microdilution and Plasmodium Lactate dehydrogenase（pLDH） assays were used to determine antioxidant,antimicrobial and antiplasmodial activities,*** assay was conducted on A+ human red blood cells and acute toxicity on male Swiss albino *** were quantitatively determined using spectrophotometric ***:The DPPH assay yielded interesting antioxidant activities of methanolic extract of Parinari curatellifolia（***） and Entada africana （***）(IC50 were 0.20±0.01μg/mL and 0.47±0.01μg/mL,respectively).This activity was highly correlated with phenolic contents of *** antimicrobial tests displayed minimal inhibitory concentrations（MICs） values ranging from 0.90 to 1.80 mg/mL for Serratia marcescens （***） the most susceptible bacterial *** value was 1.20 mg/mL for susceptible fungal strains including Mucor rouxi（***）,Fusarium oxyporum（***） and Rhizopus nigricans（***）.pLDH assay showed moderate antiplasmodial activity of Balanites aegyptiaca（***）(IC50= 24.56±3.45μg/mL),however this extract was highly haemolytic and toxic in mice(LD50= 625±128mg/kg).Conclusions:Our results support in part the use of the selected plants in the treatment of microbial *** addition the plant showed interesting antioxidant activity that could be useful in the management of oxidative stress.

关键词： Antioxidant Antimicrobial Phenolics Haemolytic

Efficiency of HAART in the prevention of mother to children HIV-1 transmission at Saint Camille medical centre in Burkina Faso,West Africa

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Asian Pacific Journal of Tropical Medicine 2012年第12期5卷 991-994页

作者： Laure Stella Ghoma Linguissi Cyrille Bisseye Tani Sagna Bolni Marius Nagalo Djeneba Ouermi Florencia W Djigma Salvatore Pignatelli Joseph D Sia Virginio Pietra Remy Moret Jean Baptiste Nikiema Jacques Simpore Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA)/LABIOGENE UFR/SVTUniversite de OuagadougouBurkina Faso department de Biologie Unirersite des Sciences et Techniques de Masuku(USTM) FrancerilleGabon Centre Medical Saint Camille OuagadougouBurkina Faso

Objective:To evaluate efficiency of HAART in the prevention of mother to child HIV ***:A longitudinal study was conducted on 1 300 women attending the antenatal service at Saint Camille Medical Centre from September 2010 to July *** HIV status of mothers was determined by rapid tests and *** results were confirmed by real-time *** was used to determine HIV status of children born from HIV-positive ***:Among 1 300 pregnant women tested for HIV,378 were *** were predominantly housewives(69.7%),and their mean age was(28.32±0.15) *** overall prevalence of HIV transmission from mother to child was 4.8%(18/378).This prevalence differed significantly from 0.0%(0/114) to 6.8%(18/264) in children born from mothers under HAART and those with mothers under New Prophylactic Protocol(ACT + 3TC + NVP),respectively(Pdetermined by PCR of children born from HIV- positive mother is necessary and recommended in the context of PMTCT in Burkina *** also found that PCR is an effective tool to confirm HIV status in pregnant women.

关键词： Pregnant Women Child HIV PCR DNA

In vitro antiplasmodial activity of crude extracts from Togolese medicinal plants

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Asian Pacific Journal of Tropical Medicine 2011年第2期4卷 129-132页

作者： Koffi Koudouvo Simplice D.Karou denise P.Ilboudo Kouami Kokou Kodjo Essien Kodjo Aklikokou Comlan de Souza Jacques Simpore Mensavi Gbeassor Centre de Recherche et de Formation sur les Plantes Medicinales(CERFOPLAM) Universite de LomeTogo Laboratoire de Botanique et d'Ecologie Vegetale Faculte des SciencesUniversite de LomeTogo Centre de recherche Biomoleculaire Pietro Annigoni(CERBA) OuagadougouBurkina Faso

Objective:To investigate the antimalarial effect of a few plants in Togo folk medicine. Methods:After ethnobotanical survey,Opilia celtidifolia,Pavetta corymbosa（***） and Tamarindus indica（***） were selected for *** vitro antimalarial tests were performed on crude extracts against fresh clinical isolates of Plasmodium falciparum using the semi ***:Different IC50,values of the extracts ranged from 2.042 to 100.000μg/mL. According to the results,the methanol extract of aerial part of *** followed by aqueous extract of fruit of *** were the most active(IC50 of 2.042 and 4.786μg/mL,respectively). Qualitative test revealed the presence of alkaloids in the leaves of *** that may be responsible for the activity of the ***:Our study provides scientific evidence for usage of plant in the folk medicine,and further studies are needed for identification and purification of the active principles.

关键词： Medicinal plants In vitro Screening Plasmodium falciparum

determination of Fusarium Species Associated with Onion Plants(Allium cepa)in Field in Burkina Faso Causing Damping-Off and Bulb Rots

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American Journal of Plant Sciences 2020年第1期11卷 64-79页

作者： Konwende Raissa Kintega P.Elisabeth Zida Romain Soalla Vianney W.Tarpaga Philippe Sankara Paco Sereme Laboratoire de phytopathologie Institute de l’Environnement et de Recherches Agricoles(INERA) OuagadougouBurkina Faso universite Joseph Ki-ZERBO OuagadougouBurkina Faso

Onion (Allium cepa L.) is the second most important vegetable crop in Burkina Faso and provides an important source of income for those involved in the sector. However, producers are facing significant pre-harvest and post-harvest losses. To date, knowledge of major diseases of the crop is limited, limiting the development of effective control strategies. The objective of this study was to test the pathogenicity of some species of Fusarium. To this end, 33 fungal isolates collected from onion plants in 17 localities and belonging to five Fusarium species were used to inoculate onion seeds and bulbs to determine the pathogenic species responsible for damping-off on seedlings and basal bulb rot in Burkina Faso. The virulence of pathogenic isolates was determined according to the percentages of seedling damping-off evaluated 28 Days After Sowing, and the extent of rot in millimetres on inoculated bulbs. The evaluation of isolates on seedlings revealed that the most pathogenic isolates belong to the species F. proliferatum (I29, I21, I37, I33, I31), F. thapsinum (I35) and F. solani (I38) which resulted in 58.33% - 70.83% of seedling damping-off. The most pathogenic isolates on bulbs belong to the species F. proliferatum (I4, I29, I32) and F. oxysporum (I52, I50, I16) which caused 21.67 to 25 mm of rot on bulbs. Isolate I29 was very virulent on both seedlings and bulbs. The isolates of F. fujikuroi species were all low pathogenic on seedlings but one of them, (I27), expressed average pathogenicity on bulbs.

关键词： Fungal Pathogen Violet de Galmi Fusarium Seedling Damping-Off Bulb Rots

Identification of Fusarium Species Associated with Onion(Allium cepa L.)Plants in Field in Burkina Faso

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Advances in Bioscience and Biotechnology 2020年第3期11卷 94-110页

作者： Konwende Raissa Kintega P.Elisabeth Zida Vianney W.Tarpaga Philippe Sankara Paco Sereme Laboratoire de Phytopathologie Institute de l’Environnement et de Recherches Agricoles(INERA) OuagadougouBurkina Faso universite Joseph Ki-ZERBO OuagadougouBurkina Faso

Many fungi limit onion production in Burkina Faso. This study aims to identify the main Fusarium species associated with onion plant in field in order to determine those involved in seedling damping-off and bulb rot, and develop adequate management strategies of these diseases. For this purpose, 36 isolates of Fusarium were isolated from onion plants in 17 sites and subjected to molecular analysis and biometric characterization. The results revealed that the isolates belong to five Fusarium species: Fusarium oxysporum (44.44% of the isolates), Fusarium proliferatum (41.66%), Fusarium solani (5.55%), Fusarium fujikuroi (5.55%) and Fusarium thapsinum (2.77%). Fusarium oxysporum, F. proliferatum, F. solani and F. fujikuroi had faster mycelial development, with a growth rate of 7.72 - 8.27 mm/d, than F. thapsinum (6.52 mm/d). Conidia of F. oxysporum, F. proliferatum and F. solani were longer (4.74 - 5.96 μm) than those of F. fujikuroi and F. thapsinum (3.20 - 4.04 μm). Fusarium solani and F. oxysporum, respectively, had the largest and most partitioned conidia.

关键词： Allium cepa Fusarium Molecular Identification Biometric Characterization Fungal Rot