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Phenotyping Key Fruit Quality Traits in Olive Using RGB Images and Back Propagation Neural Networks

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Plant Phenomics 2023年第3期5卷 484-500页

作者： Giuseppe Montanaro Angelo Petrozza Laura Rustioni Francesco Cellini Vitale Nuzzo università degli studi della basilicata 85100 PotenzaItaly ALSIA Agenzia Lucana Sviluppo Innovazione in AgricolturaMetapontum Agrobios Research Center75010 MetapontoItaly Department of Biological and Environmental Sciences and Technologies University of SalentoLecceItaly

To predict oil and phenol concentrations in olive fruit,the combination of back propagation neural networks(BPNNs)and contact-less plant phenotyping techniques was employed to retrieve RGB image-based digital proxies of oil and phenol *** of cultivars(×3)differing in ripening time were sampled(~10-day interval,×2 years),pictured and analyzed for phenol and oil *** to this,fruit samples were pictured and images were segmented to extract the red(R),green(G),and blue(B)mean pixel values that were rearranged in 35 RGB-based colorimetric *** BPNNs were designed using as input variables(a)the original 35 RGB indexes,(b)the scores of principal components after a principal component analysis(PCA)pre-processing of those indexes,and(c)a reduced number(28)of the RGB indexes achieved after a sparse *** results show that the predictions reached the highest mean R^(2) values ranging from 0.87 to 0.95(oil)and from 0.81 to 0.90(phenols)across the *** addition to the R^(2),other performance metrics were calculated(root mean squared error and mean absolute error)and combined into a general performance indicator(GPI).The resulting rank of the GPI suggests that a BPNN with a specific topology might be designed for cultivars grouped according to their ripening *** present study documented that an RGB-based image phenotyping can effectively predict key quality traits in olive fruit supporting the developing olive sector within a digital agriculture domain.

关键词： Image image supporting

Biogenetic Linkage among Ecotopes within Matera-Sassi (Italy) Habitat

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Open Journal of Genetics 2021年第4期11卷 63-76页

作者： Giovanni Figliuolo Mario Nuzzi Dipartimento di Culture Europee e del Mediterraneo (DiCEM) Università degli Studi della Basilicata Matera Italy

In the management of UNESCO cultural sites of significant environmental importance, the erroneous assumption that the environment is independent of biological heritage often prevails. The mapping of phylogenetic resources in the ancient city Matera-Sassi (MS) and the multivariable analysis at the level of ecotope and habitat have made it possible to identify the consistency of the plant genetic heritage and the biogenetic associations between the different ecotopes through the species they host. The bioclimatic variables and geomorphology of MS define an ecological niche refuge for rare or absent species in the surrounding landscape (e.g., Campanula versicolor L., Centranthus ruber (L.) Dc., Capparis spinosa L., Cymbalaria muralis Hill, Crepis spp., Lavathera arborea L.) The total floristic capital in MS amounts to 190 species belonging to 59 different botanical families;80% are native species and only 4% are invasive. About half are Mediterranean with a moderate presence (17%) of cosmopolitan and sub-cosmopolitan;5% are endemic species. Autogenic ecotopes (Type I) represented by

关键词： Biodiversity Ecotope Habitat Heritage In Situ Conservation

A realized Tuber <i>magnatum</i>niche in the upper Sinni area (south Italy)

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Open Journal of Genetics 2013年第2期3卷 102-110页

作者： Giovanni Figliuolo Giovanna Trupo Stefania Mang Dipartimento delle Culture Europee e del Mediterraneo (DICEM) Università degli Studi della Basilicata Matera Italy

A realized Tuber magnatum niche, in the upper Sinni area (Serrapotamo, south Italy), was studied using geographic information system, direct in situ survey and genetic diversity at DNA marker loci. Truffières were north facing, associated with a fine loamy-sandy soil bearing high carbonate content over a conglomerate bead-rock substrate. Target area is located in Pavari's cold and medium-cold Lauretum phithoclimatic belts. Ascoma genomic DNA was analyzed and ITS sequences, microsatellites (MA2, MA4 and MA7), along with M13 minisatellite loci, did show low heteozygosity (HE = 0.153). Serrapotamo genotypes did not express localized markers when compared with Piemonte (north Italy) genetic material. ITS marker, monomorphic within T. magnatum, was efficient at distinguishing T. borchii in a mixture of both. Serrapotamo niche represents a unique T. magnatum diversity spot in the southernmost side of the Mediterranean region. Maintaining Serrapotamo T. magnatum diversity by undertaking conservation measures is adviced.

关键词： Genetic Diversity Conservation Niche White Truffle

Addressing Biodiversity Conservation Methods with Fagus sylvatica Genetic Indicators

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Open Journal of Genetics 2014年第2期4卷 166-174页

作者： Giovanni Figliuolo Dipartimento delle Culture Europee e del Mediterraneo: Architettura Ambiente Patrimoni culturali (DiCEM) Università degli Studi della Basilicata Matera Italy

Species biological history revealed by genetic indicators can provide guidelines for long-term biodiversity conservation in Natura 2000 network. Fagus sylvatica is the keystone species which regulates in the Mediterranean Eco-Region ecosystem structure, function and composition. Six hundred fifty nine F. sylvatica individuals have been sampled across 20 sites of European interest in Southern Italy and analyzed at 5 microsatellite loci. For sites marked by both maximum heterozygosity (Ho) and minimum heterozygote deficit (Fis) (IT9210210, ITA070099, IT9210205 and IT9220075) it is suggested to avoid impacts by adopting very conservative measures. Promoting migration processes (pollen flow and seed flow) would be appropriate where it has been monitored low heterozygosity and high genetic disequilibrium. Margin effect due to dryness should be buffered with appropriate belts of thermophilus broad leaved tree species.

关键词： Fagus sylvatica Genetic Diversity Indicator Natura 2000

Box Behnken design-based optimized extraction of non-dioxin-like PCBs for GC-ECD and GC-MS analyses in milk samples

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Emerging Contaminants 2020年第1期6卷 303-311页

作者： Valeria Nardelli Valeria D’Amico Ines della Rovere Francesco Casamassima Wadir Mario Valentino Marchesiello Donatella Nardiello Maurizio Quinto Istituto Zooprofilattico Sperimentale della Puglia e della basilicata Via Manfredonia 20Foggia71121Italy Dipartimento di Scienze Agrarie degli Alimenti e dell’Ambiente-Universita degli Studi di FoggiaVia Napoli2571122FoggiaItaly Key Laboratory of Natural Resources of the Changbai Mountain and Functional Molecules Yanbian UniversityPark Road 977133002YanjiChina

A multivariate optimization process of the sample extraction procedure by Box-Behnken design through a global desirability function is described for the determination of six non-dioxin-like polychlorinated biphenyls(NDL-PCBs#28,52,101,153,138 and 180)in milk by GC-ECD and mass *** factors were involved in refining the extraction conditions:the acetone percentage in the extraction mixture,the sample/solvent ratio,and the extraction *** three-factor design required 26 experiments that were carried out in duplicate and in a randomized order to minimize the bias effects of uncontrolled *** optimized factors(acetone percentage:30%;sample-to-solvent ratio:0.11 g mL^-1;extraction time:45 min)ensured a low solvent consumption and a reduced extraction time,allowing a rapid and simultaneous preparation of multiple sample *** method was validated according to the European directives(Decision 657/2002/EC,SANTE 2017/11813/EC)through the evaluation of linearity,selectivity,LOD,LOQ,recovery,precision,and ruggedness.

关键词： NDL-PCBs Box behnken experimental design Milk GC-ECD Extraction process optimization Method validation

Agro-Biodiversity Spatial Assessment and Genetic Reserve Delineation for the Pollino National Park (Italy)

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Natural Resources 2014年第7期5卷 308-321页

作者： Giovanni Figliuolo Domenico Cerbino Agenzia Lucana di Sviluppo e Innovazione in Agricoltura Rotonda Italy Dipartimento delle Culture Europee e del Mediterraneo: Architettura Ambiente Patrimoni Culturali Università degli Studi della Basilicata Matera Italy

Ancient fruit trees, grape vines, traditional crop and garden cultivars have been inventoried in Pollino National Park by adopting a spatial sampling grid which covers 23 different municipalities (~1500 Km2) and 190 surveyed sites. Each site is a circle with a visible radius of 200 - 250 m. The spatial analysis of the diversity of plant genetic resources has been conducted with pre-fixed landscape units (size: 4 Km × 4 Km). Overall, 49 different woody long cycle (455 ancient cultivars) and 53 short cycle species (102 ancient cultivars) have been scored. Long cycle species exhibit higher cultivar richness than short cycle species. The analysis has recognized that pre-Columbian indigenous cultivars had not been displaced by the American species introduced after the 16th century. In addition, it is confirmed that small-scale poly-production, under conditions of spatial niche variation, is associated to high species and cultivar richness. The mapped realized niche, for both pre- and post-Columbian genetic resources, includes the actual genetic reserve suitable for in situ conservation of plant agro-biodiversity. Agro-biodiversity models, drivers of genetic erosion, and realistic responses to genetic erosion are outlined.

关键词： Genetic Reserve Genetic Resource Realized Niche Richness Spatial Analysis

Halophile plant growth-promoting rhizobacteria induce salt tolerance traits in wheat seedlings(Triticum aestivum L.)

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Pedosphere 2020年第5期30卷 684-693页

作者： Maryam SAFDARIAN Hossein ASKARl Ghorbanali NEMATZADEH Adriano SOFO Sari Agricultural Sciences and Natural Resources university Mazandaran ProvinceSari ***(Iran) Department of Biotechnology Faculty of New Technologies and Energy EngineeringShahid Beheshti UniversityTehran 198396941(Iran) Department of European and Mediterranean Cultures:Architecture EnvironmentCultural Heritage(DiCEM)Universita degli Studi dello BasilicataVia Lanera 20Matera 75100(Italy)

Salinity is one of the most important growth-limiting factors for most crops in arid and semi-arid regions;however,the use of plant growth-promoting rhizobacteria isolated from saline soils could reduce the effects of saline stress in *** study aimed to evaluate the efficiency of plant growth-promoting rhizobacteria(PGPRs),isolated from the rhizosphere of halophile plants,for the growth,Na^+/K^+balance,ethylene emission,and gene expression of wheat seedlings{Triticum aestivum L.)grown under saline conditions(100 mmol L^-1 NaCl)for 14 d.A total of 118 isolates obtained from saline soils of the deserts of Iran were tested for their capacity as *** of the 118 isolates,17 could solubilize phosphate(Ca3(P〇4)2),5 could produce siderophores,and 16 could synthesize indole-3-acetic ***,PGPRs were also evaluated for aminocyclopropane-l-carboxylate deaminase activity.A pot experiment was conducted to evaluate the ability of 28 PGPR isolates to promote growth,regulate Na^+/K^+balance,and decrease ethylene emissions in *** most efficient PGPRs were Arthrobacter *** atrophaeus,Enterobacter ashuriae,and Pseudomonas *** expression analysis revealed the up-regulation of H^+-PPase,HKT1,NHX7,CAT,and APX expression in roots of Enterobacter-inocuVdied salt-stressed ***-tolerant rhizobacteria exhibiting plant growth-promoting traits can facilitate the growth of wheat plants under saline *** results indicate that the isolation of these bacteria may be useful for formulating new inoculants to improve wheat cropping systems in saline soils.

关键词： ethylene emission gene expression growth-promoting trait indole-3-acetic acid phosphate solubility saline soil siderophore

Compost and Compost Tea Management of Mini Watermelon Cultivations Affects the Chemical, Physical and Sensory Assessment of the Fruits

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Agricultural Sciences 2015年第1期6卷 117-125页

作者： Loredana Liguori Catello Pane Donatella Albanese Giuseppe Celano Massimo Zaccardelli Marisa Di Matteo Dipartimento di Ingegneria Industriale Università di Salerno Fisciano Italy Consiglio per la Ricerca e la Sperimentazione in Agricoltura Centro di Ricerca per l’Orticoltura Pontecagnano Italy Dipartimento delle Culture Europee e del Mediterraneo: Architettura Ambiente Patrimoni Culturali (DICEM) Università degli Studi della Basilicata Matera Italy

Compost, rich in plant nutrients, is a readily available fertilizer with beneficial effects on physical, chemical, biochemical and biological properties of the soils. Moreover compost-based treatments can exert protective effects against plant diseases occurrence and/or stimulate an enhanced plant physiological status with improvements in quantity and quality of crop productions. In this study the effects of three different compost-based cropping managements on the productive response and main quality parameters of watermelon fruits were investigated. Treatments, in comparison with the conventional cultivation method, were: soil amendment with an agricultural waste compost (AWC), a municipal waste compost (MWC) and a foliar treatment with a compost tea blend (CTB). The productive responses and colour parameters related to compost treatments did not show significant differences compared to control ones, which reached a total yield of about 10.22 kg·m-2 with a mean weight of 2.74 kg. AWC caused a higher ascorbic acid content with an increase of 50% than conventional treatment, while fruits obtained by CTB showed higher values in firmness and Quality Index than control samples. The analysis of main sugars highlighted that the application of compost as biofertilizer influenced the ratio among fructose, glucose and sucrose with respect to those observed for control fruits.

关键词： Biostimulants Compost Quality Watermelon

In Vivo Phenotyping for the Early Detection of Drought Stress in Tomato

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Plant Phenomics 2019年第1期1卷 142-151页

作者： Michela Janni Nicola Coppede Manuele Bettelli Nunzio Briglia Angelo Petrozza Stephan Summerer Filippo Vurro Donatella Danzi Francesco Cellini Nelson Marmiroli Domenico Pignone Salvatore Iannotta Andrea Zappettini Institute of Materials for Electronics and Magnetism(IMEM) National Research Council(CNR)Parco Area delle Scienze 37/A43124 ParmaItaly Institute of Bioscience and Bioresources(IBBR) National Research Council(CNR)Via Amendola 165/A70126 BariItaly universitàdegli studi della basilicata Dipartimento delle Culture Europee e del Mediterraneo:ArchitetturaAmbientePatrimoni Culturali(DICEM)Via S.RoccoI-75100 MateraItaly ALSIA Centro Ricerche Metapontum Agrobios s.s.Jonica 106km 4482MetapontoMT 75010It aly 5Department of Chemistry Life Sciences and Environmental SustainabilityUniversity of ParmaParco Area delle Scienze11/A43124 ParmaItaly

Drought stress imposes a major constraint over a crop yield and can be expected to grow in importance if the climate change predicted comes *** methods are needed to facilitate crop management via the prompt detection of the onset of ***,we report the use of an in vivo OECT(organic electrochemical transistor)sensor,termed as bioristor,in the context of the drought response of the tomato *** device was integrated within the plant’s stem,thereby allowing for the continuous monitoring of the plant’s physiological status throughout its life *** was able to detect changes of ion concentration in the sap upon drought,in particular,those dissolved and transported through the transpiration stream,thus efficiently detecting the occurrence of drought stress immediately after the priming of the defence *** bioristor’s acquired data were coupled with those obtained in a high-throughput phenotyping platform revealing the extreme complementarity of these methods to investigate the mechanisms triggered by the plant during the drought stress event.

关键词： drought thereby prompt