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To eat or get heat： Behavioral trade-offs between thermoregulation and feeding in gregarious necrophagous larvae

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Insect Science 2018年第5期25卷 883-893页

作者： Jana Podhorna Cindy Aubernon Marie Borkovcova Julien Boulay Valery Hedouin Damien Charabidze department of Zoology Fisheries Hydrobiology and Apiculture Mendel University in Brno Czech Repubfic Unite de Taphonomie Medico-Legale University of Lille CHU Lille EA France

The thermoregulation behavior ofLucilia sericata larvae （Diptera： Calliphori- dae）, a necrophagous species that feeds on vertebrate cadavers, was investigated. These larvae require high heat incomes to develop, and can elevate temperatures by forming large aggregates. We hypothesized that L. sericata larvae should continue to feed at temperatures up to 38 ℃, which can be reached inside larval masses. Thermal regulation behavior such as movement between a hot food spot and colder areas was also postulated. The hypotheses were tested by tracking for 1 h the activity of single, starved third instar larvae in a Petri dish containing 1 food spot （FS） that was heated to a constant temperature of 25 ℃, 34 ℃ or 38 ℃ with an ambient temperature of 25 ℃. The influence of previous conspecific activity in the food on larval behavior was also tested. The crops of larvae were dissected to monitor food content in the digestive systems. Based on relative crop measurements, larvae fed at all food temperatures, but temperature strongly affected larval behavior and kinematics. The total time spent by larvae in FS and the duration of each stay decreased at high FS temperature. Previous activity of conspecifics in the food slightly increased the time spent by larvae in FS and also decreased the average distance to FS. Therefore, necrophagous L. sericata larvae likely thermoregulate during normal feeding activities by adjusting to local fluctuations in temperature, particularly inside maggot masses. By maintaining a steady internal body temperature, larvae likely reduce their development time.

关键词： blowflies Calliphoridae forensic entomology foraging thermoregulation video tracking

Quality Assessment of Transcriptome Data Using Intrinsic Statistical Properties

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Genomics, Proteomics & Bioinformatics 2010年第1期8卷 57-71页

作者： Guillaume Brysbaert Franois-Xavier Pellay Sebastian Noth Arndt Benecke Institut des Hautes études Scientifiques & Institut de Recherche Interdisciplinaire(CNRS USR3078 Université de Lille1)91440 Bures-sur-YvetteFrance

In view of potential application to biomedical diagnosis, tight transcriptome data quality control is compulsory. Usually, quality control is achieved using labeling and hybridization controls added at different stages throughout the processing of the biologic RNA samples. These control measures, however, only reflect the performance of the individual technical manipulations during the entire process and have no bearing as to the continued integrity of the RNA sample itself. Here we demonstrate that intrinsic statistical properties of the resulting transcriptome data signal and signal-variance distributions and their invariance can be identified independently of the animal species studied and the labeling protocol used. From these invariant properties we have developed a data model, the pa- rameters of which can be estimatei：l from individual experiments and used to compute relative quality measures based on similarity with large reference datasets. These quality measures add supplementary, non-redundant in- formation to standard quality control estimates based on spike-in and hybridization controls, and are exploitable in data analysis. A software application for analyzing datasets as well as a reference dataset for AB 1700 arrays are provided. They should allow AB 1700 users to easily integrate this method into their analysis pipeline, and might instigate similar developments for other transcriptome platforms.

关键词： transcriptome microarray quality control distribution properties statistics software

Comparisons between numerical calculations and measurements in vaned diffuser of SHF impeller

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排灌机械工程学报 2013年第12期31卷 1013-1020页

作者： Annie-Claude Bayeul-Lainé Patrick Dupont Giovanna Cavazzini Patrick Cherdieu Antoine Dazin Gérard Bois Olivier Roussette Laboratoire de Mécanique de lille(UMR CNRS 8107) Arts et Métiers ParisTech Laboratoire de Mécanique de lille(UMR CNRS 8107) Ecole Centrale de LilleVilleneuve D'Ascq Cedex 59651France department of Industrial Engineering University of Padova

The paper presents analysis of the performance and the internal flow behaviour in the vaned diffuser of a radial flow pump using PIV(particle image velocimetry)and pressure probe *** measurements have already been performed at middle height inside one diffuser channel passage for a given speed of rotation and various mass flow *** results have been already presented in several previous *** experiments have been performed using a three-hole pressure probe traverses from hub to shroud diffuser width at different radial locations between the two diffuser geometrical *** simulations are also realized with the commercial codes Star CCM+7.02.011 and *** rotor and fully unsteady calculations of the whole pump have been *** between numerical results,previous experimental PIV results and new probe traverses one's are presented and discussed for one mass flow *** this respect,a first attempt to take into account fluid leakages between the rotating and fixed part of the pump has been checked since it may affects the real flow structure inside the diffuser.

关键词： vaned diffuser radial flow pump unsteady flow numerical calculations particle image velocimetry(PIV) three-hole probe

Performance comparison of chlorinated chiral stationary phases in supercritical fluid chromatography for separation of selected pyrrolidone derivatives

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Journal of Pharmaceutical Analysis 2019年第4期9卷 248-253页

作者： Anca-Elena Dascalu Alina Ghinet Muriel Billamboz Emmanuelle Lipka Univ. lille Inserm U995 - LIRIC - Lille Inflammation Research International Center F-59000 Lille France Ecole des Hautes Etudes d'Ingenieur (HEI) Laboratoire de Pharmacochimie 13 rue de Toul F-59046 Lille France UFR Pharmacie Laboratoire de Chimie Analytique BP 83 F-59006 Lille France 'Alexandru Ioan Cuza’ university of Iasi Faculty of Chemistry Bd. Carol I nr. 11 700506 Iasi Romania

The effects of two chlorinated chiral stationary phases, namely, Lux Cellulose-2 and Lux i-Cellulose-5, flow-rate, percentage of co-solvent and chemical structures of the compounds on retention and resolution were studied within this article. In this work a backpressure of 150 bar, a temperature of 40 ℃ and 10% of methanol as co-solvent were chosen as operating conditions. The optimum flow-rate was 2 mL/min. The percentage of co-solvent was studied between 7.5% and 15%.We have observed that 15% of methanol gave the best results for most of the compounds. For all the derivatives, the Lux Cellulose-2 provided better resolutions going from 1.50 to 3.59 compared with Lux i-Cellulose-5.

关键词： Antibacterial compounds Chirality Enantiomers Flow-rate Particle size

Intrauterine insemination with donor sperm:only the number of motile spermatozoa inseminated influences both pregnancy and live-birth rates

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Asian Journal of Andrology 2022年第3期24卷 287-293页

作者： Marie Cardey-Lefort Berengere Ducrocq Audrey Uk Helen Behal Anne-Laure Barbotin Geoffroy Robin Reproductive Biology Institute Lille University HospitalLille F-59037France lille university Lille University HospitalULR 2694-METRICSLille F-59000France Endocrine Gynecology and Reproductive Medicine Unit Lille University HospitalLille F-59037France

Intrauterine insemination with donor sperm(IUI-D)is an assisted reproductive technology(ART)offered to couples with definitive male infertility or risk of genetic disease ***,we sought to evaluate our practice in IUI-D and identify factors that influenced the success *** performed a retrospective,single-center study of all IUI-D procedures performed at lille university Medical Center(lille,France)between January 1,2007,and december 31,*** and multivariate analyses with a mixed logistic model were used to identify factors associated with clinical pregnancies and live *** included 322 couples and 1179 IUI-D *** clinical pregnancy rate was 23.5%,and the live birth rate was 18.9%per *** a multivariate analysis,the women’s age was negatively associated with the live birth *** number of motile spermatozoa inseminated was the only factor associated with both clinical pregnancies and live births,with a chosen threshold of 0.75 *** clinical pregnancy and live birth rates were,respectively,17.3%and 13.0%below the number of motile spermatozoa inseminated threshold and 25.9%and 21.0%at or above the threshold(all P=0.005).The number of motile spermatozoa inseminated was the only factor that significantly influenced both pregnancies and live-birth rates after ***,below a threshold of 0.75 million motile spermatozoa inseminated,those rates were significantly *** of this number of motile spermatozoa inseminated threshold may help centers to allocate donations more effectively while maintaining reasonable waiting times for patients.

关键词： intrauterine insemination male infertility number of motile spermatozoa inseminated sperm donor

Rotational dynamics of bottom-heavy rods in turbulence from experiments and numerical simulations

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Theoretical & Applied Mechanics Letters 2021年第1期11卷 26-31页

作者： Linfeng Jiang Cheng Wang Shuang Liu Chao Sun Enrico Calzavarini Center for Combustion Energy Key Laboratory for Thermal Science and Power Engineering of Ministry of EducationDepartment of Energy and Power EngineeringTsinghua UniversityBeijing 100084China department of Engineering Mechanics School of Aerospace EngineeringTsinghua UniversityBeijing 100084China universitéde lille ULR 7512 Unitéde Mécanique de Lille-Joseph Boussinesq(UML)F 59000 LilleFrance

We successfully perform the three-dimensional tracking in a turbulent fluid flow of small axisymmetrical particles that are neutrally-buoyant and bottom-heavy,i.e.,they have a non-homogeneous mass distribu-tion along their symmetry *** experimentally show how a tiny mass inhomogeneity can affect the particle orientation along the preferred vertical direction and modify its tumbling *** experiment is complemented by a series of simulations based on realistic Navier-Stokes turbulence and on a point-like particle model that is capable to explore the full range of parameter space characterized by the gravi-tational torque stability number and by the particle aspect *** propose a theoretical perturbative prediction valid in the high bottom-heaviness regime that agrees well with the observed preferential ori-entation and tumbling rate of the *** also show that the heavy-tail shape of the probability distribution function of the tumbling rate is weakly affected by the bottom-heaviness of the particles.

关键词： Particle-laden flows Turbulent flows Direct numerical simulations Particle tracking

A TDG/CBP/RARα Ternary Complex Mediates the Retinoic Acid-dependent Expression of DNA Methylation-sensitive Genes

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Genomics, Proteomics & Bioinformatics 2014年第1期12卷 8-18页

作者： Hélène Léger Caroline Smet-Nocca Amel Attmane-Elakeb Sara Morley-Fletcher Arndt G.Benecke Sebastian Eilebrecht Vaccine Research Institute INSERM U955Institut Mondor de Recherche Biomédicale Institut des Hautes tudes Scientifiques Unité de Glycobiologie Structurale et Fonctionnelle–CNRS UMR 8576 Group of NMR and Structural BiologyUniversité Lille 1 Unité de Glycobiologie Structurale et Fonctionnelle–CNRS UMR 8576 Neuroplasticity TeamUniversité Lille 1 CNRS UMR 7224 Université Pierre et Marie Curie

The thymine DNA glycosylase （TDG） is a multifunctional enzyme,which is essential for embryonic *** mediates the base excision repair （BER） of G：T and G：U DNA mismatches arising from the deamination of 5-methyl cytosine （5-MeC） and cytosine,*** studies have pointed at a role of TDG during the active demethylation of 5-MeC within CpG *** interacts with the histone acetylase CREB-binding protein （CBP） to activate CBP-dependent *** addition,TDG also interacts with the retinoic acid receptor α （RARα）,resulting in the activation of RARα target *** we provide evidence for the existence of a functional ternary complex containing TDG,CBP and activated RARα.Using global transcriptome profiling,we uncover a coupling of de novo methylation-sensitive and RA-dependent transcription,which coincides with a significant subset of CBP target *** introduction of a point mutation in TDG,which neither affects overall protein structure nor BER activity,leads to a significant loss in ternary complex stability,resulting in the deregulation of RA targets involved in cellular networks associated with DNA replication,recombination and *** thus demonstrate for the first time a direct coupling of TDG＇s epigenomic and transcription regulatory function through ternary complexes with CBP and RARα.

关键词： CREB-binding protein Thymine DNA glycosylase Retinoic acid receptor α Transcription regulation Cytosine DNA methylation Epigenomics

HCV-specific immune responses induced by CIGB-230 in combination with IFN-α plus ribavirin

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World Journal of Gastroenterology 2014年第1期20卷 148-162页

作者： Yalena Amador-Caizares Gillian Martínez-Donato Liz lvarez-Lajonchere Claudia Vasallo Mariacarla Dausá Daylen Aguilar-Noriega Carmen Valenzuela Ivette Raíces Jean Dubuisson Czeslaw Wychowski Zurina Cinza-Estévez Marlén Castellanos Magdalys Núez Anny Armas Yaimé González Ismariley Revé Ivis Guerra ngel Pérez Aguiar Santiago Dueas-Carrera Centro de Ingeniería Genética y Biotecnología Playa 10600HavanaCuba Center for Infection and Immunity of lille (CIIL) Inserm U1019CNRS UMR8204Institut Pasteur de LilleUniv Lille Nord de FranceF-59000 LilleFrance Instituto de Gastroenterología Vedado 10400HavanaCuba Hospital Clínico Quirúrgico "Hermanos Amejeiras" Havana 10200Cuba Centro de Inmunoensayos Havana 11300Cuba

AIM: To analyze hepatitis C virus (HCV)-specific immune responses in chronically infected patients under triple therapy with interferon-α (IFN-α) plus ribavirin and CIGB-230.

关键词： Clinical trial DNA vaccine Enzyme-linked immunospot Hepatitis C virus Leukopenia

A frequency and velocity-dependent impedance method for prediction of rail/foundation dynamics

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Earthquake Engineering and Engineering Vibration 2021年第1期20卷 101-111页

作者： Reda Mezeh Marwan Sadek Fadi Hage Chehade Isam Shahrour Laboratory of Civil Engineering and GeoEnvironment University of LilleSciences and TechnologiesPolytech’LilleVilleneuve d’AscqHauts-de-France 59655France Doctoral School of Science and Technology Lebanese UniversityBeirut 0000Lebanon

This paper presents an efficient numerical tool for the prediction of railway dynamic response.A behavior calibration of the infinite Euler-Bernoulli beam resting on continuous viscoelastic foundation is *** laws of the discrete elements are determined for a rectilinear ballasted track.A three-dimensional model coupled with an adaptive meshing scheme is employed to calibrate the beam model impedances by finding the similarity between the output signals using the genetic *** model shows an important performance with significant reduction in computational *** study emphasizes the major impact of the excitation characteristics on the parameters of the discrete models.

关键词： moving loads rail vibrations rail/foundation interaction dynamic impedances genetic algorithm