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Journal of Integrative Plant Biology 2010年第10期52卷 933-940页

作者： Neila Jellouli Asma Ben Salem Abdelwahed Ghorbel Hatem Ben Jouira Laboratoire de physiologie Moléculaire de la Vigne Centre de Biotechnologie de Borj Cédria

An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis （2-de） for recalcitrant plant species, in particular for grapevine （Vitis vinifera L.）. Trichloroacetic acid-acetone （TCA-acetone） and phenol extraction methods were evaluated for proteome analysis of leaves and roots from the Tunisian cultivar ＇Razegui＇. The phenol-based protocol proved to give a higher protein yield, a greater spot resolution, and a minimal streaking on 2-de gels for both leaf and root tissues compared with the TCA-based protocol. Furthermore, the highest numbers of detected proteins on 2-de gels were observed using the phenol extraction from leaves and roots as compared with TCA-acetone extraction.

关键词： Proteins Protein products Extraction Extracts Acetone Tca Separating Methods Colloids Roots

Evaluation of androgenic activity of Zingiber officinale and Pentadiplandra brazzeana in male rats

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Asian Journal of Andrology 2002年第4期4卷 299-301页

作者： PierreKamtchouing GisèleYolandeMbongueFandio ThéophileDimo Hermine Boukeng Jatsa Laboratoire de physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounLaboratoire de Physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounLaboratoire de Physiologie Animale Faculté des Sciences Université de Yaoundé I B.P.812 Yaoundé CamerounDépartement de Biologie et de Physiologie Animales Faculté des Sciences Université de Douala B.P. 24157 Douala Cameroun

Aim: Aqueous extracts of Zingiber officinale and Pentadiplandra brazzeana were tested for their possible androgenic activity in male Wistar rats. Methods: The aqueous extracts of the two plants were gavaged separately to 2 groups of rats at a similar dose of 600 ***^***-1 for 8 days. At the end of the treatment, the animals were killed and the blood, testis, epididymis, seminal vesicles and prostate were collected for biochemical analysis. Results: The aqueous extract of Z. officinale significantly increased in the relative weight of the testis, the serum testosterone level, testicular cholesterol level and epididymal α-glucosidase activity. The aqueous extract of P. brazzeana significantly increased the weights of the testis, seminal vesicles and prostate. It also significantly increased the serum and testicular testosterone level. The fructose, α-glucosidase and cholesterol levels in P. brazzeanatreated rats were increased by 28%, 35% and 114%, respectively. Conclusion: The aqueous extracts of both P. brazzeana and Z. officinale have an androgenic activity, which seems to be more potent with P. brazzeana than with Z. officinale. (Asian J Androl 2002 dec; 4: 299-301)

关键词： P. brazzeana Z. officinale testosterone androgenic activity

Pharmacological attenuation of blood pressure variability

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Acta Pharmacologica Sinica 2005年第11期26卷 1288-1289页

作者： Claude JULIEN France Laboratoire de physiologie Faculte de Pharmacie Lyon 69373 Universite Claude Bernard Lyon 1

Over the past few years, the research team of ProfessorDing-feng SU has reported an impressive quantity of experimental data about the relationships between blood pressurevariability (BPV) and end-organ damage, a topic of obviousclinical interest. This research work has been summarized ina paper that appeared in the August issue of the renownedjournal Trends in Pharmacological Sciences[1]. The studiesby Su et al provide convincing evidence that BPV is an independent cardiovascular risk factor that should be considered as such and, therefore, might become an important target for therapeutic interventions. Besides these exciting perspectives in the prevention and treatment of cardiovascular

关键词： BPV Pharmacological attenuation of blood pressure variability

Regulation of Sulfate Uptake and Assimilation——the Same or Not the Same？

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Molecular Plant 2010年第2期3卷 314-325页

作者： Jean-Claude Davidian Stanislav Kopriva unite mixte de recherche Biochimie et Physiologie Molculaire des Plantes Montpellier SupAgro/CNRS/INRA/UM2 2 place Pierre Viala 34060 MontpellierCedex 2 France department of Metabolic Biology John Innes Centre Norwich NR4 7UH UK

Plant take up the essential nutrient sulfur as sulfate from the soil, reduce it, and assimilate into bioorganic compounds, with cysteine being the first product. Both sulfate uptake and assimilation are highly regulated by the demand for the reduced sulfur, by availability of nutrients, and by environmental conditions. In the last decade, great prog- ress has been achieved in dissecting the regulation of sulfur metabolism. Sulfate uptake and reduction of activated sulfate, adenosine 5＇-phosphosulfate （APS）, to sulfite by APS reductase appear to be the key regulatory steps. Here, we review the current knowledge on regulation of these processes, with special attention given to similarities and differences.

关键词： Metabolic regulation nutrient and metal transport nutrition primary metabolism sulfate uptake sulfur metabolism.

Wastewater Treatment Potentials of Vegetated Beds with Brillantaisia *** Hutch&Dalz and Polygonum salicifolium Brouss ex Wild in the Western Highlands of Cameroon

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Journal of Environmental Protection 2019年第3期10卷 389-412页

作者： Julius Kajoh Boyah Theophile Fonkou Telesphore Benoit Nguelefack Victor Francois Nguetsop Martin Lekeufack unite de Recherche de Botanique Appliquee(URBOA) Faculty of ScienceUniversity of DschangDschangCameroon unite de Recherche de physiologie Animales et Phytopharmacologie(URPHYPHA) Faculty of ScienceUniversity of DschangDschangCameroon

The objective of this study was to evaluate the potentials of beds vegetated with medicinal species (Brillantaisia bauchiensis and Polygonum salicifolium) in a constructed wetland for domestic wastewater treatment in the Western Highlands of Cameroon. The study was carried out between March and September 2017 on plants collected from a natural wetland in Penka-Michel. The two plants species selected based on their ethnobotanical importance were transplanted and allowed to grow to maturity in a prepared natural wetland at Penka-Michel and a constructed wetland for domestic wastewater treatment on the campus of the University of Dschang. Growth parameters were followed for the two plants species in both wetlands. The physicochemical parameters and faecal bacteria concentrations were measured only for the vegetated and non-vegetated/control beds in the constructed wetland. Overall, the two plants species showed increased growth in height, diameter, leaf number and plants density. The change in diameter and density were very significantly influenced by species type in the constructed wetland than in the natural wetland. Generally, plant growth in height, diameter and density were higher with B. bauchiensis in the constructed wetland than with P. salicifolium in both wetlands. The mean faecal bacteria removal was higher in the vegetated beds for some bacteria than in the non-vegetated/control bed. There was a significant difference in the reduction efficiency of TSS, turbidity, BOD, Faecal streptococci and Total coliforms bacteria between the inflow and the outflow of some treatment beds especially the bed vegetated with Brillantaisia bauchiensis. There were correlations between the two plants species as concerns increased plants height, diameter, leave number, shoot number and nutrients uptake in the constructed wetland beds compared with the natural wetland.

关键词： Wastewater Vegetated Beds Medicinal Macrophytes Brillantaisia bauchiensis and Polygonum salicifolium

Effects of Cadmium on Polar Lipid Composition and Unsaturation in Maize(Zea mays) in Hydroponic Culture

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Journal of Integrative Plant Biology 2007年第12期49卷 1693-1702页

作者： Radhouane Chaffai Tinni Nouhou Seybou Brahim Marzouk Ezzedine EI Ferjani Laboratoire de Bio-physiologie Cellulaires Vegetales Faculte des Sciences de Bizerte 7021 Zarzouna-BizerteTunisie Laboratoire ERTAC physiologie et Genetique Vegetales 63177 AubiereClermont FerrandFrance Laboratoire d'Adaptation et d'Amelioration des Plantes Institut National de la Recherche Scientifique et Technique(INRST)BP 95-2050 Hammam-LifTunisie

This study aimed to evaluate the effect of Cd exposure （100 μmol/L） on polar lipid composition, and to examine the level of fatty acid unsaturation in maize （Zea mays L.）. In roots, the level of 16：0 and monounsaturated fatty acids （16：1 ＋ 18：1） decreased in phosphatidylcholine （PC） and phosphatidylethanolamine （PE）. In contrast, the proportion of unsaturated 18-C fatty acid species showed an opposite response to Cd. The content, on the other hand, of PC, PE, digalactosyldiacylglycerol （DGDG）, and steryl lipids increased in roots （2.9-, 1.6-, 5.3-, and 1.7-fold increase, respectively）. These results suggest that a more unsaturated fatty acid composition than found in control plants with a concomitant increase in polar lipids may favor seedling growth during Cd exposure. However, the observed increase in the steryl lipid （SL）： phospholipid （PL） ratio （twofold）, the decrease in monogalactosyldiacylglycerol （MGDG）： DGDG ratio, as well as the induction of lipid peroxidation in roots may represent symptoms of membrane injury. In shoots, the unsaturation level was markedly decreased in PC and phosphatidylglycerol （PG） after Cd exposure, but showed a significant increase in sulfoquinovosyldiacylglycerol （SQDG）, MGDG and DGDG. The content of PG and MGDG was decreased by about 65%, while PC accumulated to higher levels （4.4-fold increase）. Taken together, these changes in the polar lipid unsaturation and composition are likely to be due to alterations in the glycerolipid pathway. These results also support the idea that the increase in overall unsaturation plays some role in enabling the plant to withstand the metal exposure.

关键词： cadmium fatty acids heavy metal stress lipid peroxidation polar lipids unsaturation level Zea mays.

Why implantation of bio-loggers may improve our understanding of how animals cope within their natural environment

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Integrative Zoology 2019年第1期14卷 48-64页

作者： Marie-Amélie FORIN-WIART Manfred R.ENSTIPP Yvon LE MAHO Yves HANDRICH Universitéde Strasbourg CNRSIPHCDépartement EcologiePhysiologie et EthologieUMR 7178StrasbourgFrance Centre d’Etudes Biologiques de Chizé CNRSUMR 7372Villiers en BoisFrance Centre Scientifique de Monaco Département de Biologie PolaireMonaco

Bio-loggers are miniaturized autonomous devices that record quantitative data on the state of free-ranging ani-mals(***,position and physiology)and their natural *** is especially relevant for spe-cies where direct visual observation is difficult or ***,ongoing technical development allows the monitoring of numerous parameters in an increasing range of species over extended ***,the ex-ternal attachment of devices might affect various aspects of animal performance(energetics,thermoregulation,foraging as well as social and reproductive behavior),which ultimately affect *** attachment might also increase entanglement risk and the conspicuousness of animals,leaving them more vulnerable to *** contrast,implantation of devices can mitigate many of these undesirable effects and might be prefera-ble,especially for long-term studies,provided that the many challenges associated with surgical procedures can be *** may then allow us to gather data that would be impossible to obtain otherwise and thereby may provide new and ecologically relevant insights into the life of wild ***,we:(i)discuss the pros and cons of attachment methods;(ii)highlight recent field studies that used implanted bio-loggers to address eco-physiological questions in a wide range of species;and(iii)discuss logger implantation in light of ethical considerations.

关键词： behavior bio-logging device implantation eco-physiology long-term deployment

Novel role for transcranial magnetic stimulation to study post-traumatic respiratory neuroplasticity

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Neural Regeneration Research 2016年第7期11卷 1073-1074页

作者： Stéphane Vinit Michel Petitjean Universite de Versailles Saint-Quentin-en-Yvelines UFR des Sciences de la Sante-Simone Veil Montigny-le-Bretonneux France U1179 INSERM End:icap Laboratoire de Physiologie TITAN UFR des Sciences de la Sante-Simone Veil Montigny-le-Bretonneux France Service de physiologie-Explorations Fonctionnelles Hopital Ambroise Pare Assistance Publique-Hopitaux de Paris Groupe HospitalierParis-Ile de France Ouest Boulogne-BiUancourt France

Transcranial magnetic stimulation-a tool used in humans：Transcranial magnetic stimulation（TMS）is a non-invasive widespread clinical tool used to stimulate cortical areas in human *** technique utilizes a brief,high... 详细信息

关键词： stimulation transcranial traumatic cortical Weber cortex reorganization brief injured neuronal

Post-Translational Regulation of AtFER2 Ferritin in Response to Intracellular Iron Trafficking during Fruit development in Arabidopsis

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Molecular Plant 2009年第5期2卷 1095-1106页

作者： Karl Ravet Brigitte Touraine Sun A. Kim Francoise Cellier Sebastien Thomine Mary Lou Guerinot Jean-Francois Briat Frederic Gaymard Biochimie et physiologie Moleculaire des Plantes (B&PMP) Unite Mixte de Recherche CNRS INRA Universite Montpellier 2 SupAgro Place Viala Bat. 7 F-34060 Montpellier France department of Biological Sciences Dartmouth College 6044 Gilman Hall Hanover NH 03755 USA Institut des Sciences du Vegetal CNRS Bat 23 Avenue de la Terrasse 91198 Gif-sur-Yvette France

Ferritins are major players in plant iron homeostasis. Surprisingly, their overexpression in transgenic plants led only to a moderate increase in seed iron content, suggesting the existence of control checkpoints for iron loading and storage in seeds. This work reports the identification of two of these checkpoints. First, measurement of seed metal content during fruit development in Arabidopsis thaliana reveals a similar dynamic of loading for Fe, Mn, Cu, and Zn. The step controlling metal loading into the seed occurs by the regulation of transport from the hull to the seed. Second, metal loading and ferritin abundance were monitored in different genetic backgrounds affected in vacuolar iron transport （AtVIT1, AtNRAMP3, AtNRAMP4） or plastid iron storage （AtFER1 to 4）. This approach revealed （1） a post-translational reg- ulation of ferritin accumulation in seeds, and （2） that ferritin stability depends on the balance of iron allocation between vacuoles and plastids. Thus, the success of ferritin overexpression strategies for iron biofortification, a promising approach to reduce iron-deficiency anemia in developing countries, would strongly benefit from the identification and engineering of mechanisms enabling the translocation of high amounts of iron into seed plastids.

关键词： Nutrient and metal transport iron transport ferritin post-transcriptional control biofortification Arabidopsis.

Gαq,Gγ1 and Plc21C Control Drosophila Body Fat Storage

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Journal of Genetics and Genomics 2014年第5期41卷 283-292页

作者： Jens Baumbach Yanjun Xu Philip Hehlert Ronald P.Khnlein Abteilung Molekulare Entwicklungsbiologie Forschungsgruppe Molekulare Physiologie Max-Planck-Institut fr biophysikalische Chemie Abteilung Molekulare Entwicklungsbiologie Forschungsgruppe Molekulare PhysiologieMax-Planck-Institut fr biophysikalische Chemie

mobilization of body fat is essential for energy homeostasis in animals. In insects, the adipokinetic hormone （Akh） systemically controls body fat mobilization. Biochemical evidence supports that Akh signals via a G protein-coupled receptor （GPCR） called Akh receptor （AkhR） using cyclic-AMP （cAMP） and Ca2＋ second messengers to induce storage lipid release from fat body cells. Recently, we provided genetic evidence that the intracellular calcium （iCa2＋） level in fat storage cells controls adiposity in the fruit fly Drosophila melanogaster. However, little is known about the genes, which mediate Akh signalling downstream of the AkhR to regulate changes in iCa2＋. Here, we used thermogenetics to provide in vivo evidence that the GPCR signal transducers G protein α q subunit （Gαq）, G protein γ1 （Gγ1） and Phospholipase C at 21C （Plc21C） control cellular and organismal fat storage in Drosophila. Transgenic modulation of Gαq, Gγ1 and Plc21C affected the iCa2＋ of fat body cells and the expression profile of the lipid metabolism effector genes midway and brummer, which results in severely obese or lean flies. Moreover, functional impairment of Gαq, Gγ1 and Plc21C antagonised Akh-induced fat depletion. This study characterizes Gαq, Gγ1 and Plc21C as anti-obesity genes and supports the model that Akh employs the Gαq/Gγ1/Plc21C module of iCa2＋ control to regulate lipid mobilization in adult Drosophila.

关键词： Plc21C Gαq Gγ1 Calcium Fat storage Adipokinetic hormone