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Asian Pacific Journal of Tropical Biomedicine 2012年第8期2卷 602-607页

作者： M Govindarajan R Sivakumar Division of vector biology and phytochemistry Department of ZoologyAnnamalai University

Objective:To determine repellent activity of hexane,ethyl acetate,benzene,chloroform and methanol extract of Cardiospermum halicacabum,(***) against Culex quinque/ascialus(***),Aedes aegypti(***) and Anopheles slephensi(***). Methods:Evaluation was carried out in a net cage(45 cm×30 cm×25 cm) containing 100 blood starved female mosquitoes of three mosquito species and were assayed in the laboratory condition by using the protocol of WHO 2005;The plant leaf crude extracts of *** was applied at 1.0,2.5,and 5.0 me/cm^2 separately in the exposed area of the fore *** ethanol served as ***:In this observation,the plant crude extracts gave protection against mosquito bites without any allergic reaction to the test person,and also,the repellent activity was dependent on the strength of the plant *** tested plant crude extracts had exerted promising repellent against all the three ***:From the results it can be concluded the crude extract of *** was potential for controlling ***, *** and *** mosquitoes.

关键词： Repellency Cardiospermum halicacabum Culex quinquefasciatus Aedes aegypti Anopheles stephensi

Evaluation of Andrographis paniculata Burm.f.(***) extracts against Culex quinquefasciatus(Say.) and Aedes aegypti(Linn.)(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第3期4卷 176-181页

作者： Marimuthu Govindarajan Division of vector biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608 002Tamil NaduIndia

Objective:To investigate the larvicidal and ovicidal efficacy of different extracts of Andrographis paniculata（***） against Culex quinquefasciatus（***） Say and Aedes aegypti（*** ） L（Diptera:Culicidae）.Methods:Larvicidal efficacy of the crude leaf extracts of *** with five different solvents like benzene,hexane,ethyl acetate, methanol and chloroform was tested against the early third instar larvae of *** and *** ovicidal activity was determined against two mosquito species to various concentrations ranging from 50-300 ppm under the laboratory ***:The benzene, hexane,ethyl acetate,methanol and chloroform leaf extract of *** was found to be more effective against *** than *** LC50 values were 112.19,137.48, 118.67,102.05,91.20 ppm and 119.58,146.34,124.24,110.12,99.54 ppm *** five tested solvent,methanol and ethyl acetate crude extract was found to be most effective for ovicidal activity against two mosquito *** extract of methanol and ethyl acetate exerted 100%mortality at 200 ppm against *** and at 250 ppm against ***. Conclusions:From the results it can be concluded the crude extract of *** was a potential for controlling *** and *** mosquitoes.

关键词： Andrographis paniculata larvicidal activity Ovicidal activity Culex quinquefasciatus Aedes aegypti

Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第2期4卷 106-111页

作者： Marimuthu Govindarajan Division of vector biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608 002Tamil NaduIndia

Objective:To investigate the larvicidal and repellent properties of essential oils from various parts of four plant species Cymbopogan citrates,Cinnamomum zeylanicum,Rosmarinus of ficinalis and Zingiber of ficinale against Culex tritaeniorhynchus（***） and Anopheles subpictus（***）.Methods:Essential oils were obtained by hydro-distillation *** mosquitoes were reared in the vector control laboratory and twenty five late third ins tar larvae of *** and *** were exposed to based on the wide range and narrow range test,essential oil tested at various concentrations ranging from 25 to 250 *** larval mortality was observed after 24 h under the laboratory *** repellent efficacy was determined against two mosquito species at three concentrations viz., 1.0,2.5 and 5.0 mg/cm2 under laboratory ***:Results showed all the four plant essential oil produced significant larval mortality against two mosquito ***,the highest larvicidal activity was observed in the essential oil from Zingiber of ficinale against Cx. tritaeniorhynchus and *** with the LC50 and LC90 values as 98.83,57.98 ppm and 186.55, 104.23 ppm,*** the four essential oil shows significant repellency against Cx. tritaeniorhynchus than *** four essential oil tested the highest repellency was observed in Zingiber of ficinale,a higher concentration of 5.0 mg/cm2 provided 100%protection up to 150 and 180 min against *** and ***,***: In this work,it can be concluded that four essential oils which were distilled from Cymbopogan citrates,Cinnamomum zeylanicum,Rosmarinus of ficinalis and Zingiber of ficinale showed promising larvicidal and repellent agent against *** and ***.

关键词： Cymbopogan citrates Cinnamomum zeylanicum Rosmarinus officinalis Zingiber officinale Essential ois Larvicidal activity Repellent activity Mosquito vectors

Mosquito larvicidal and ovicidal properties of Eclipta alba(L.) Hassk(Asteraceae) against chikungunya vector,Aedes aegypti(Linn.)(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第1期4卷 24-28页

作者： M Govindarajan P Karuppannan Division of phytochemistry and vector biology Department of ZoologyAnnamalai UniversityAnnamalai Nagar-608 002India

Objective:The present study deals with the investigation of larvicidal and ovicidal activities of benzene,hexane,ethyl acetate,methanol and chloroform leaf extract of Eclipta alba（***） against dengue vector,Aedes aegypti（***）.Methods:Twenty five earlyⅢinstar larvae of *** was exposed to various concentrations（50-300 ppm） and was assayed in the laboratory by using the protocol of WHO 2005;the 24 h LC50 values of the *** leaf extract was determined by Probit *** ovicidal activity,slightly modified method of Su and Mulla was *** ovicidal activity was determined against *** to various concentrations ranging from 100-350 ppm under the laboratory *** egg hatch rates were assessed 48 h post ***:The LC50 values of benzene,hexane,ethyl acetate,methanol and chloroform extract of *** against early third instar larvae of *** were 151.38,165.10, 154.88,127.64 and 146.28 ppm,*** larvicidal activity was observed in the methanol extract followed by chloroform,benzene,ethyl acetate and hexane *** mortality was observed in *** five solvent tested the methanol extract was found to be most effective for ovicidal activity against *** methanol extracts exerted 100%mortality （zero hatchability） at 300 ***:From the results it can be concluded the crude extract of *** was an excellent potential for controlling *** mosquito.

关键词： Eclipta alba Aedes aegypti Larvicidal activity Ovicidal activity

Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) *** Benth (Rutaceae) against three mosquito species

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Asian Pacific Journal of Tropical Medicine 2010年第11期3卷 874-877页

作者： Marimuthu Govindarajan Division of phytochemistry and vector biology Department of ZoologyAnnamalai UniversityAnnamalai Nagar-608 002India

Objective:To determine the mosquito larvicidal activity of leaf essential oil and their chemical constituents from Clausena anisata（***）（Willd.） *** *** Culex quinquefasciatus,Aedes aegypti and Anopheles ***:Essential oil was obtained by hydro-distillation and the chemical composition of the leaf essential oil was analyzed using gas chromatography—mass *** mosquitoes were reared in the vector control laboratory and twenty lateⅢinstar larvae of three mosquito species were exposed to based on the wide range and narrow range tests,essential oil was tested at 50.100.150,200 and 250 ppm and each compound was tested at various concentration（5-75 ppm） and were assayed in the laboratory by using the protocol of WHO 2005:the 24 h LC50 values of the *** leaf essential oil and their major compounds were determined following Probit ***:The oil contained were mainlyβ-pinene（32.8%）,sabinene（28.3%）,germacrene-D（12.7%）,estragole （6.4%） and linalool（5.9%）.The essential oil from the leaves of *** exhibited significant larvicidal activity,with 24 h LC50 values of 140.96,130.19 and 119.59 *** five pure constituents extracted from the *** leaf essential oil were also tested individually against three mosquito *** LC50 values ofβ-pinene,sabinene,germacrene-D,estragole and linalool appeared to be most effective against Anopheles stephensi(LC50-23.17,19.67,16.95. 11.01,35.17 ppm) followed by Aedes aegypti(LC50-27.69,21.20,18.76,12.70,38.64 ppm) and Culex quinquefasciatus(LC50-32.23,25.***.21.28,14.01,42.28).Conclusions:The essential oil of C. anisata contains five major compounds and has remarkable larvicidal properties,which may be considered as a potent source for the production of natural larvicides.

关键词： Mosquito vectors Clausena anisata Essential oil GC-MS analysis Larvicidal activity

Larvicidal and repellent activities of Sida acuta Burm.F.(Family:Malvaceae) against three important vector mosquitoes

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Asian Pacific Journal of Tropical Medicine 2010年第9期3卷 691-695页

作者： Marimuthu Govindarajan Division of phytochemistry and vector biology Department of ZoologyAnnamalai UniversityAnnamalai Nagar-608 002India

Objective:To determine the larvicidal and repellent activities of Sida acuta Burm.F.（Family: Malvaceae）extract against Culex quinquefasciatus,Aedes aegypti and Anopheles stephensi. Methods:Twenty five late III instar larve of three mosquito species were exposed to various concentrations（15-90 mg/L） and were assayed in the laboratory by using the protocol of WHO 2005:the 24 h LC50 values of the Sida acuta leaf extract was determined following Probit analysis. The repellent efficacy was determined against three mosquito species at three concentrations viz.,1.0,2.5 and 5.0 mg/cm2 under the laboratory ***:Results showed varying degree of larvicidal activity of crude extract of Sida acuta against three important mosquitoes with LC50 values ranging between 38 to 48 mg/*** crude extract had strong repellent action against three species of mosquitoes as it provided 100%protection against Anopheles stephensi for 180 min followed by Aedes aegypti（150 min） and Culex quinquefasciatus（120 min）.Conclusions: From the results it can be concluded the crude extract of Sida acuta was an excellent potential for controlling Culex quinquefasciatus,Aedes aegypti and Anopleles stephensi mosquitoes.

关键词： Sida acuta Mosquito vectors Larvicidal activity Repellent activity

Mosquito adulticidal and repellent activities of botanical extracts against malarial vector,Anopheles stephensi Liston(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2011年第12期4卷 941-947页

作者： Marimuthu Govindarajan Rajamohan Sivakumar Division of vector biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalai Nagar - 608 002Tamil NaduIndia

To determine the adulticidal and repellent activities of different solvent leaf extracts of Eclipta alba（***） and Andrographis paniculata（***） against malarial vector, Anopheles stephensi（***）Mathods:Adultieidal efficacy of the crude leaf extracts of *** *** with five different solvents like benzene,*** acetate. methanol and chloroform was tested against the five to six dav old adult female mosquitoes of *** adult mortality was observed after 24 h under the laboratory *** repellent efficacy was determined against An,stephensi mosquito species at three concentrations viz.,1.0,2.5 and 5.0 mg/cm2 under laboratory ***:Among the tested solvents the maximum efficacy was observed in the methanol *** LC50 and LC90 values of *** and *** against adults of *** were 150.***.130.19 ppm and 285.***.244.16 ppm. *** mortality was obsened in *** chi-square values were significant at P<0.05 lev *** extract of *** and *** was produce maximum repellency against ***:From the results it can be concluded the crude extract of E. nihil and *** was an excellent potential for controlling *** mosquitoes.

关键词： Adulticidal activity Repellent activity Anopheles stephensi Eclipta alba Andrographis paniculata

Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus,Aedes aegypti and Anopheles stephensi(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 43-48页

作者： Govindarajan M Mathivanan T Elumalai K Krishnappa K Anandan A Division of vector biology and phytochemistry Department of ZoologyAnnamalai University unit of Entomotoxicity Department of ZoologyGovernment Thirumagal Mills College

Objective:To determine the ovicidal and repellent activities of methanol leaf extract of Ervatamia coronaria(***) and Caeslpinia pulckerrima(***) against Culex quinquefasciatus(***),Aedes aegypti(***) and Anopheles stephensi(An. stephensi).Methods:The ovicidal activity was determined against three mosquito species at various concentrations ranging from 50-450 ppm under the laboratory *** hatch rates were assessed 48 h after *** repellent efficacy was determined against three mosquito species at three concentrations viz.,1.0,2.5 and 5.0 mg/cm under the laboratory conditions. Results:The crude extract of *** exerted zero hatchability(100%mortality) at 250.200 and 150 ppm for ***,*** and ***,*** crude extract of *** exerted zero hatchability(100%mortality) at 375.300 and 225 ppm for ***,*** and ***,*** methanol extract of E. coronaria found to be more repellenct than *** extract.A higher concentration of 5.0 mg/cm^2 provided 100%protection up to 150.180 and 210 min against ***,Ae. aegypti and ***,*** results clearly showed that repellent activity was dose ***:From the results it can be concluded the crude extracts of *** and *** are an excellent potential for controlling ***,*** and *** mosquitoes.

关键词： Ervatamia coronaria Caesalpinia pulcherrima Ovicidal activity Repellent activity Culex quinquefasciatus Aedes aegypti Anopheles stephensi Mosquito Leaf extract Mortality

Mosquito larvicidal properties of Impatiens balsamina(Balsaminaceae)against Anopheles stephensi,Aedes aegypti and Culex quinquefasciatus(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2014年第3期 222-224页

作者： Marimuthu Govindarajan Mohan Rajeswary unit of vector biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagarIndia

Objective:To assess the larvicidal potential of the crude benzene,chloroform,ethyl acetate and methanol solvent extracts of the medicinal plant Impatiens balsamina against Anopheles stephensi(***),Aedes aegypti(***)and Culex quinquefasciatus(***).Methods:Twenty five third instar larvae of ***,*** and *** were exposed to various concentrations and were assayed in the laboratory by using the protocol recommended by *** larval mortality was observed after 24 h of ***:Among extracts tested,the highest larvicidal activity was observed in leaf methanol extract of Impatiens balsamina against ***,*** and *** with the LC_(50)and LC_(90)values 98.04,119.68,125.06 and 172.93,210.14,220.60 mg/L,***:From the results it can be concluded that the larvicidal effect of Impatiens balsamina against ***,*** and *** make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.

关键词： Impatiens balsamina Larvicidal activity Anopheles stephensi Aedes aegypti Culex quinquefasciatus

Mosquito repellent potential of Pithecellobium dulce leaf and seed against malaria vector Anopheles stephensi(Diptera:Culicidae)

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Journal of Coastal Life Medicine 2016年第3期4卷 236-239页

作者： Mohan Rajeswary Marimuthu Govindarajan unit of vector biology and phytochemistry Department of ZoologyAnnamalai UniversityAnnamalainagar-608002TamilnaduIndia

Objective:To determine the repellent properties of hexane,benzene,ethyl acetate,chloroform and methanol extract of Pithecellobium dulce(***)leaf and seed against Anopheles stephensi(***).Methods:Repellent activity assay was carried out in a net cage(45 cm×30 cm×25 cm)containing 100 blood starved female mosquitoes of *** assay was carried out in the laboratory conditions according to the WHO 2009 *** crude extracts of *** were applied at 1.0,2.5,and 5.0 mg/cm^(2) separately in the exposed fore arm of study *** was used as the sole ***:In this study,the applied plant crude extracts were observed to protect against mosquito *** were no allergic reactions experienced by the study *** repellent activity of the extract was dependent on the concentration of the *** the tested solvents,the leaf and seed methanol extract showed the maximum *** highest concentration of 5.0 mg/cm^(2) leaf and seed methanol extract of *** provided over 180 min and 150 min protection,***:Crude extracts of *** exhibit the potential for controlling malaria vector mosquito ***.

关键词： Repellent activity Pithecellobium dulce Leaf Seed Anopheles stephensi