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A ferroptosis-inducing iridium(III) complex

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Science China Chemistry 2020年第1期63卷 65-72页

作者： Xiuxiu Wang Feng Chen Jingyi Zhang Jianxuan Sun Xinyang Zhao Yuelu Zhu Wei Wei Jing Zhao Zijian Guo State Key Laboratory of Coordination Chemistry Institute of Chemistry and Biomedical SciencesSchool of Life SciencesNanjing UniversityNanjing 210023China School of Chemistry and chemical Engineering Nanjing UniversityNanjing 210093China

Ferroptosis is a recently emerging non-apoptotic mode of cell death involving the production of iron-dependent reactive oxygen species(ROS).Here we described a mitochondria-targeted iridium(III)complex Ir FN that exhibited potent antiproliferative activity against a variety of cancer cells,especially the A2780 human ovarian cancer cells,through the ferroptosis *** studies by label-free quantitative proteomics profiling indicated that heme oxygenase 1(HMOX1)-mediated ferroptosis process was activated by Ir *** study on iron-dependent cell death,ROS accumulation,lipid peroxidation,and over released iron further confirmed the ferroptosis processes.m RNA transcription quantification,in vitro over-expression of HMOX1,and RNAi-mediated knock-down experiments suggested that Ir FN activated the over-expression of *** report revealed the first case of anticancer iridium complex leading to ferroptosis,highlighting ferroptosis as a promising approach in future design of metallodrugs.

关键词： iridium proteome ferroptosis heme oxygenase 1

Metallomics:An integrated biometal science

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Science China Chemistry 2009年第12期52卷 2055-2070页

作者： GE RuiGuang1,2 & SUN HongZhe1 1 Department of Chemistry and Open Laboratory of chemical Biology,The University of Hong Kong,Hong Kong,China 2 Laboratory of Integrative Biology,College of Life Sciences,Sun Yat-Sen University,Guangzhou 510006,China Department of Chemistry and Open Laboratory of chemical Biology The University of Hong Kong Hong Kong China Laboratory of Integrative Biology College of Life Sciences Sun Yat-Sen University Guangzhou China

Metallomics is an emerging scientific area integrating the research fields related to the understanding of the molecular mechanisms of metal-associated life processes and the entirety of metal and metalloid species within a cell or tissue type. In metallomics,metalloproteins,metalloenzymes and other metal-containing biomolecules in a biological system are referred to as metallomes,similar to genomes and proteomes in genomics and proteomics,respectively. This review discusses the concept of metallomics with a focus on analytical techniques and methods,particularly the so-called hyphenated techniques which combine a high-resolution separation technique (gel electrophoresis/laser ablation,chromatography or capillary electrophoresis) with a highly sensitive detection method such as elemental (inductively coupled plasma,ICP) or molecular (electron spray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI)) mass spectrometry,or nuclear X-ray fluorescence/absorption spectrometry. The applications of these advanced analytical methods in the identification of metallo-/phospho-/seleno-proteins,probing of relationships between structure and function of metal-loproteins,and study of clinically used metallodrugs will be selectively outlined,along with their advantages and limitations.

关键词： metallomics metallome metallodrug metalloprotein chemical speciation

An Analysis of Protein Bioinformatics Datasets Related to LewyBodies of Parkinson's Disease

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An Analysis of Protein Bioinformatics Datasets Related to Le...

Molecularly Imprinted Polymer Based on GO-QDs as Enhanced Fluorescent Nanoscale Device for Specific Recognition of Target Protein

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Xing-An LI Lin-Sen HU Key Laboratory for Honeybee Genetics and (Queen) Breeding Jilin Provincial Institute of Apicultural Science Laboratory for proteomics Department of Neurology First Affiliated Hospital Jilin University

Lewy bodies(LBs) in dopaminergic neurons in substantia nigra pars compacta(SNpc) arethe cardinal pathological hallmark of Parkinson’ disease(PD), while LBs and/ or Lewy neuritesare also seen in non-dopaminergic neurons in other brain regions of patients with PD,such as noradrenergic neurons in locus coeruleus(LC), and cholinergic neurons in prefrontalcortex(PFC) and temporal cortex(TC). To explicit protein contents of LBs in PD,here is an article that demonstrates five aspects of protein constitution of LBs by analyzingprotein bioinformatics datasets related to LBs in PD: 1, LBs is filled with 2 types of alpha-synuclein(α-SYN) positive fibrilar aggregates and/or 6 types of α-SYN positive nonfibrilaraggregates(described frequently in literature as oligomers);2, modification formsof pathological α-SYN in LBs are often associated with 5 species of chemical modifiers;3,19 α-SYN-associated proteins rich in LBs are respectively colocalized with α-SYN;4, 117 previously reported proteins in LBs in immunochemistry study are at least classified into 10 different groups of functional proteins with close relationship;5, proteomics database asprotein candidate resource for LBs contain 84, 124 and 120 proteins identified respectivelyfrom raw tissue samples of LC, SNpc and PFC regions, and 108 proteins identified from acellular separation of analyte captured from TC region, and 29 proteins identified from asub-cellular fraction of analyte isolated from TC region. It is suggested that the aforementionedaspects comprehensively and fundamentally reflect protein composition of LBs inPD.

关键词： Parkinson’ disease Lewy bodies proteins bioinformatics

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Journal of Donghua University(English Edition) 2014年第5期31卷 603-608页

作者： DING Zhao-qiang GONG Xiao TAO Lei QUAN Jing NIE Hua-li ZHU Li-min College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China National Engineering Research Center for Dyeing and Finishing of Textiles Donghua UniveLity Shanghai 201620 China

In this work the enhanced molecularly imprinted optosensing material based on graphene oxide-quantum dots （ GO- QDs） was synthesized for highly selective and sensitive specific recognition of the target protein, bovine serum albumin （BSA）. Here, GO was introduced to enhance the efficiency of mass-transfer in recognition of target protein. Molecularly imprinted polymer coated GO-QDs using BSA as template （BMIP-coated GO-QDs ） exhibited a fast mass-transfer speed, which could be ascribed to the high volume of efficient surface area and high target recognition efficiency of the synthesized nanoscale device. Under optimal conditions, it was found that the BSA as target protein could remarkably quench the relative fluorescence intensity of BMIP- coated GO-QDs linearly in a concentration-dependent manner that was best described by a Stern-Volmer equation. The Ksv （Stern- Volmer constant） for template BSA was much higher than bovine hemoglobin （BHb） and lysozyme （Lyz）, implying a highly selective recognition ability of the BMIP-coated GO-QDs to BSA. This enhanced fluorescent nanoscale device may provide opportunities to develop a system that is efficient and effective and has potential in the design of highly effective fluorescent receptor for recognition of target protein in Droteomics studies.

关键词： molecularly imprinted polymer (MIP) graphene oxide(GO) quantum dots (QDs) protein: fluorescent nanoscale device

Rhodamine B doped silica nanoparticle labels for protein microarray detection

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Science China Chemistry 2010年第4期53卷 747-751页

作者： WANG Ying1, LI ZhongHui1, ZHONG WenYing2, LI Hui2, XU DanKe1 & CHEN HongYuan1 1Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education School of Chemistry and chemical Engineering, Nanjing University, Nanjing 210093, China 2Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China Key Laboratory of Analytical Chemistry for Life Science Ministry of Education School of Chemistry and chemical Engineering Nanjing University Nanjing China Department of Analytical Chemistry China Pharmaceutical University Nanjing China

A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule. The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays, in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanoparticles were sequentially conjugated. The calibration curve is linear over the range from 800 fg to 500 pg and the limit of detection is 100 fg, which is 8 times lower than that of streptavidin-labeled Cy3 fluorescent dyes. The dyedoped SiO2 nanoparticles show potentials for the protein array detection.

关键词： Rhodamine B SiO2 nanoparticles protein detection protein microarrays

Facile synthesis of thiazole cation functionalized core/shell magnetic microspheres for isolation of hemoglobin

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Facile synthesis of thiazole cation functionalized core/shel...

Recent advances in proteolysis and peptide/protein separation by chromatographic strategies

中国化学会第十九届全国有机分析及生物分析学术研讨会

作者： Juanqiang Wang Binghai Wang Quan Li Yun Wei State Key Laboratory of chemical Resource Engineering Beijing University of Chemical Technology

＜正＞The selective separation of protein targets from complex biological samples is currently a hot topic in key life science disciplines（e.g.,diagnostics,proteomics,and protein purification）.Among these existing protocols,magnetic composite micro-（nano）spheres have attracted extensive attention due to their unique magnetic

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Science China Chemistry 2010年第4期53卷 685-694页

作者： ZHANG XiangMin1, LIU BaoHong1, ZHANG LiHua2, ZOU HanFa2, CAO Jing1, GAO MingXia1, TANG Jia1, LIU Yun1, YANG PengYuan1 & ZHANG YuKui2 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China 2Dalian Institute of chemical Physics, Chinese Academy of Sciences, Dalian 116023, China 1Department of Chemistry and Institutes of Biomedical Sciences Fudan University Shanghai 200433 China. 2Dalian Institute of chemical Physics Chinese Academy of Sciences Dalian 116023 China.

This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.

关键词： proteolysis enzymatic digestion chromatographic separation peptide enrichment mass spectrometry

A comprehensive review of edible bird nests and swiftlet farming

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Journal of Integrative Medicine 2016年第6期14卷 415-428页

作者： Lee Suan Chua Siti Najihah Zukefli Metabolites Profiling Laboratory Institute of Bioproduct DevelopmentUniversiti Teknologi Malaysia Department of Bioprocess and Polymer Engineering Faculty of Chemical and Energy EngineeringUniversiti Teknologi Malaysia

Edible bird＇s nest （EBN） is currently widely consumed by the Chinese community as tonic food and functional food, which is believed to have many medicinal benefits. Some studies have reported the biochemical compositions of EBN, graded on the basis of colour, nitrate and nitrite contents. Other studies have shown significant biological effects, while ongoing research is in progress to explore potential pharmacological applications. The high demand for EBNs in the global market has forced the local regulatory bodies to monitor swiftlet farming activities, including the EBN cleaning process. Furthermore, numerous techniques have been developed to authenticate EBN; proteomics is likely to be the most promising of these methods. However, there are limited numbers of relevant protein sequences deposited at the database. More research is needed at the molecular level to explore the mechanisms behind the biological functions, such as bone strength improvement, skin rejuvenation, epidermal growth factor activity and cell *** current and future prospects of EBN and swiftlet farming are critically reviewed in this article.

关键词： edible bird's nest swiftlet farming proteomics authentication Aerodramus

Fully automated sample treatment method for high throughput proteome analysis

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Science China Chemistry 2021年第2期64卷 313-321页

作者： Huiming Yuan Zhongpeng Dai Xiaodan Zhang Baofeng Zhao Hongwei Chu Lihua Zhang Yukui Zhang CAS Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic R&A CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Zhang Dayu School Dalian University of TechnologyDalian116024China

The bottom-up strategy for proteome analysis typically employs a multistep sample preparation workflow that suffers from being time-consuming and sample loss or contamination caused by the off-line manual ***,we developed a hollow fibre membrane(HFM)-aided fully automated sample treatment(FAST)*** to the confinement effects of HFMs and the immobilized enzymatic reactor,the proteome samples could be denatured,reduced,desalted and digested within 8–20 min via the one-stop *** method also showed superiority in trace sample *** one and half hours,we could identify about 1,600 protein groups for 500 HeLa cells as the starting materials,1.5–8 times more than those obtained by previously reported *** the on-line combination of FAST with nano-liquid chromatography-electrospray ionization tandem mass spectrometry(nanoLC-ESI-MS/MS),we further established a fully integrated platform for label-free quantification of proteome with high reproducibility and ***,FAST presented here represents a major advance in the high throughput sample treatment and quantitative analysis of proteomes.

关键词： proteomics fully automated sample treatment hollow fibre membrane immobilized enzymatic reactor label-free quantification