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Gene expression changes in spleens of the wildlife reservoir species,Eurasian wild boar(Sus scrofa),naturally infected with Brucella suis biovar 2

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Journal of Genetics and Genomics 2010年第11期37卷 725-736页

作者： Ruth C.Galindo Pilar M.Muoz María J.de Miguel Clara M.Marin Javier Labairu Miguel Revilla José M.Blasco Christian Gortazar José de la Fuente Instituto de Investigación en Recursos Cinegéticos IREC(CSIC-UCLM-JCCM) unidad de sanidad animal Centro de Investigación y Tecnología Agroalimentaria(CITA) ITG Ganadero Avda. Serapio Huici 2231610 VillabaNavarraSpain departamento de Patología animal Facultad de VeterinariaUniversity of Zaragoza department of Veterinary Pathobiology Center for Veterinary Health SciencesOklahoma State University

Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. In this study, the differ- ential gene expression profile was characterized in spleens of Eurasian wild boar naturally infected with B. suis bv. 2. Of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly （fold change 〉 1.8; P 〈 0.01） upregulated and downregulated, respectively, in infected wild boar. The analysis was focused on genes that were over represented after conditional test for biological process gene ontology. Upregulated genes suggested that B. suis bv. 2 infection induced cell maturation, migration and/or proliferation in infected animals. The genes downregulated in infected wild boar impaired the activity of several important cellular metabolic pathways such as metabolism, cytoskeleton organization and biogenesis, immune response and lysosomal function and vesicle-mediated transport. In addition, the response to stress, sperm fertility, muscle development and apoptosis seemed to be also impaired in infected animals. These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen.

关键词： Brucella suis boar genomics microarray systems biology

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Antimicrobial Resistance Genes in animal Manure, Manure-Amended and Nonanthropogenically Impacted Soils in Spain

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Advances in Bioscience and Biotechnology 2018年第9期9卷 469-480页

作者： Fernando Esperón Carlos Sacristán Matilde Carballo Ana de la Torre Grupo de Epidemiología y sanidad Ambiental Centro de Investigación en Sanidad Animal (INIA-CISA) Valdeolmos Spain

Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended [n = 8] and nonmanure-amended [n = 8]). All samples were collected in central Spain. detection was based on 18 selected antimicrobial resistance genes (ARGs). The most commonly detected genes in animal manure were sul1 (16/16), sul2 (16/16), tet(A) (16/16), aadA (16/16), tet(B) (15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8) were more frequently detected in chicken manure, whereas pig slurry samples presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the four genes selected for their clinical relevance, three—blaCTX-M, vanA, and mecA—were detected in animal manure. The blaCTX-M (1/8) and vanA (5/8) genes were only identified in chicken manure. To our knowledge, this is the first report of direct detection of mecA gene in poultry manure and pig slurry. Eleven out of 18 ARGs were detected in amended soil, while only genes sul2 (3/8) and str (2/8) were found in nonanthropogenically impacted soils (NAIS), supporting the hypothesis that ARGs may serve as indicators of “anthropogenic impact” on the environment.

关键词： Antibiotic Resistance blaCTX-M mecA Pig Slurry vanA

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Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus

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Insect Science 2015年第2期22卷 178-190页

作者： Noemi Hernandez Jose A. Escudero Alvaro San Millan Bruno Gonzalez-Zorn Jorge M. Lobo Jose R. Verdu Monica Suarez department sanidad animal Facultad de Veterinaria Universidad Complutense de Madrid Avenida Puerta de Hierro sin Madrid CP28040 department Biogeografia y Cambio Global Museo Nacional de Ciencias Naturales CSIC Jose Gutierrez Abascal 2 Madrid28006 I.U.I. CIBIO (Centro Iberoamericano de la Biodiversidad) Universidad de Alicante Carretera de San Vicente del Raspeigsin Alicante 03080 Spain

Unlike other dung beetles, the Iberian geotrupid, Thorectes lusitanicus, exhibits polyphagous behavior; for example, it is able to eat acorns, fungi, fruits, and carrion in addition to the dung of different mammals. This adaptation to digest a wider diet has physiological and developmental advantages and requires key changes in the composition and diversity of the beetle＇s gut microbiota. In this study, we isolated aerobic, facultative anaerobic, and aerotolerant microbiota amenable to grow in culture from the gut contents of T. lusitanicus and resolved isolate identity to the species level by sequencing 16S rRNA gene fragments. Using BLAST similarity searches and maximum likelihood phylogenetic analyses, we were able to reveal that the analyzed fraction （culturable, aerobic, facultative anaerobic, and aerotolerant） of beetle gut microbiota is dominated by the phyla Pro- teobacteria, Firmicutes, and Actinobacteria. Among Proteobacteria, members of the order Enterobacteriales （Gammaproteobacteria） were the most abundant. The main functions associated with the bacteria found in the gut of T. lusitanicus would likely include nitrogen fixation, denitrification, detoxification, and diverse defensive roles against pathogens.

关键词： actinobacteria firmicutes geotrupidae gut microbiota polyphagy,proteobacteria

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development of a chromatographic lateral flow immunoassay for detection of African swine fever virus antigen in blood

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animal Diseases 2022年第3期2卷 139-153页

作者： Daniel W.Madden Sun-Young Sunwoo Natasha N.Gaudreault Jessie D.Trujillo Igor Morozov Carmina Gallardo Juergen A.Richt department of Diagnostic Medicine/Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKSUSA Centro de Investigación en sanidad animal(CISA-INIA/CSIC) MadridSpain

African swine fever(ASF)is a highly lethal disease of domestic and wild swine caused by African swine fever virus(ASFV).The disease currently circulates in Africa,Europe,Asia and on the island of *** ongoing epizootics in Europe and Asia have produced millions of animal deaths and severe economic *** effective vaccine is available for ASF,making rapid and accurate detection of ASFV essential for disease mitigation *** available diagnostics for ASFV possess significant limitations related to assay performance,deployability,and/or turn-around time;therefore there is an unmet need for pen-side diagnostic tests with sufficient sensitivity and specificity.A chromatographic lateral flow immunoassay(LFIA)was developed for the detection of ASFV antigen in EDTA-treated whole blood using monoclonal antibodies targeting the viral p30 *** assay requires only water to perform and provides results in 25 min,making it well-suited for field *** LFIA was capable of detecting genotype I and genotype II strains of ASFV in EDTA blood from experimentally infected pigs at varying time-points after infection,though it was unable to detect a genotype X ASFV *** sensitivity correlated with clinical disease severity,body temperature,and viral DNA levels,and was over 90%in animals showing moderate to severe ASF-related symptoms after challenge with virulent genotype II *** LFIA also showed a robust diagnostic specificity of over 98%,which is essential to field testing for a high consequence to foregin animal *** LFIA targeting the viral p30 protein can reliably detect ASFV in whole blood from animals showing moderate to severe clinical signs of infection with virulent genotype I and II isolates,making it a promising candidate for use as a field-deployable antigen detection *** evaluation using field samples and different virus strains is required to further assess the utility of this rapid diagnostic test.

关键词： African swine fever Lateral flow immunoassay Point of care diagnostics

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Functional explanation of extreme hatching asynchrony: Male Manipulation Hypothesis

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Zoological Research 2022年第5期43卷 843-850页

作者： Manuel Soler Francisco Ruiz-Raya Lucia Sanchez-Perez Juan Diego Ibanez-Álamo Juan JoseSoler departamento de Zoologia Facultad de CienciasUniversidad de GranadaGranada E-18071Spain unidad Asociada Coevolucion:Cucos Hospedadores y Bacterias SimbiontesUniversidad de GranadaGranada E-18071Spain Centro de Investigacion Mariña Universidade de VigoGrupo de Ecoloxia AnimalVigo 36310Spain departamento de Ecologia Funcional y Evolutiva Estacion Experimental de ZonasÁridas(CSIC)Almeria E-4120Spain

Hatching asynchrony in birds is considered an adaptation to facilitate brood reduction because under conditions of food scarcity, the smallest nestling usually dies soon after hatching, thereby minimizing parental effort. However, in species with extreme hatching asynchrony, the last hatchlings paradoxically experience a very low probability of survival and death can take so long that it can hardly be considered an adaptation. Here, we propose and experimentally tested a new adaptive hypothesis explaining the brood reduction paradox, namely the“Male Manipulation Hypothesis”. Our hypothesis suggests that by inducing asynchronous hatching,females increase the feeding requirements of the brood, which will induce males to increase provisioning effort. In addition, females may extend the period of male manipulation by feeding the smallest nestling just enough to sustain life. Our study showed that male common blackbirds(Turdus merula) increased their effort(i.e., number of food items per hour) in experimental asynchronous broods compared to synchronous broods, while females reduced their contribution, as predicted by the hypothesis.

关键词： Brood reduction Food allocation Hatchingasynchrony Malemanipulation hypothesis Sexual differences in food allocation Turdus merula

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Epidemiological Aspects and Clinicopathological Findings in Cats Naturally Infected with Feline Leukemia Virus (FeLV) and/or Feline Immunodeficiency Virus (FIV)

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Open Journal of Veterinary Medicine 2012年第1期2卷 13-20页

作者： Victorio M. Collado Ana Domenech Guadalupe Miró Sonsoles Martin Elena Escolar Esperanza Gomez-Lucia departamento Medicina y Cirugía animal Facultad de Veterinaria Universidad Complutense Madrid Spain departamento sanidad animal Facultad de Veterinaria Universidad Complutense Madrid Spain

Infections produced by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), two of the most prevalent pathogens in cats, range from passing unnoticed to presenting a wide variety of clinical signs. Different epidemiological, clinical, hematological and virological parameters were analyzed in 78 FIV- and/or FeLV-infected cats. FeLV-infected (FeLV+) cats were considerably younger than FIV-infected (FIV+) cats, and in general were seen to have a more severe disease than FIV+ cats. Around one third of the cats presented anemia, and neutropenia was also frequently observed. Though a higher percentage of FIV+ than FeLV+ cats had altered leukocyte counts, FeLV+ cats had altered counts of both neutrophils and lymphocytes more frequently than FIV+, which usually presented only either altered neutrophils or lymphocyte counts. Virological markers were only detected in FeLV+cats, either as mono- or dual-infection, and correlated with the severity of the disease, but not in FIV+ cats. In conclusion, these results suggest that FeLV affects more blood cell types and provokes death of affected animals at a much earlier age than FIV and that the severity of the disease seemed to depend on the viral status of the cat.

关键词： FIV FeLV Epidemiology Hemogram CD4:CD8 Ratio

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Global stability for multi-species Lotka-Volterra cooperative systems： One hyper-connected mutualistic-species

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International Journal of Biomathematics 2015年第3期8卷 215-223页

作者： Cruz Vargas-de-Leon unidad de Medicina Experimental Hospital General de Mexico"Dr. Eduardo Liceaga" DF 06726 Mexico

We propose two models of one hyper-connected mutualistic-species described by delay differential equations of Lotka-Volterra type. An hyper-connected model comprises a central species interacting with a number of peripheral species around it, that is to say, one animal species （pollinators or dispersers） that interacts with several plant species （flowering plants or fruit trees）, or several animal species that interact with one plant species. We derive a necessary and sufficient condition for the global asymptotic stability of the unique coexisting steady state of hyper-connected systems by means of novel Lyapunov functionals.

关键词： Lotka Volterra cooperative system discrete delays distributed delays Lyapunov functional global stability.

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The Relationship between Shell Morphology and Crevice Size Affecting Retreat Selection of the Keeled Box Turtle(Cuora mouhotii)

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Asian Herpetological Research 2020年第4期11卷 342-349页

作者： Fanrong XIAO Zhen HONG Jichao WANG Taggert BUTTERFIELD Haitao SHI Key Laboratory for Ecology of Tropical Islands of Ministry of Education Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikou 571158HainanChina Escuela Nacional de Estudios Superiores unidad Morelia Universidad Nacional Autonoma de MexicoMorelia 58190Mexico

Rock crevices are important retreat sites for many *** lizards and snakes,crevicedwelling turtles have rigid shells which severely limit the size and shape of crevices they are able to *** studies have addressed the relationship between turtle's shell morphology,behavior,and crevice ***,we investigate the relationship between morphology and crevice size selection in the Keeled box turtle(Cuora mouhotii).We radio tracked turtles in the field to document crevice dimensions and turtle behavior associated with crevices,and we conducted a controlled experiment in the lab to understand how a turtle's morphology influences its decision to select crevices with different *** laboratory and field experiments demonstrate that turtles select deep and moderately wide crevices that are low enough to barely accommodate the *** used by turtles tend to be about 2 times as wide,1.2 times as high,and 3 times longer than the turtle's ***,turtles tended to prefer crevices with horizontal openings in the field and position themselves head first in the *** conclude that morphology,especially carapace height,influences the Keeled box turtles' decision to select specific sizes of *** argue that the tendency for turtles to select crevices that come into contact with their carapace contributes to successful defense against predators,as it would be more difficult for a predator to remove a turtle when the crevice is nearly touching the turtles shell.

关键词： antipredation burrow microhabitat rock crevice shelter

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Mesenchymal stem cell-derived exosomes promote neurogenesis and cognitive function recovery in a mouse model of Alzheimer’s disease

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Neural Regeneration Research 2019年第9期14卷 1626-1634页

作者： Edwin E. Reza-Zaldivar Mercedes A. Hernández-Sapiéns Yanet K. Gutiérrez-Mercado Sergio Sandoval-ávila Ulises Gomez-Pinedo Ana L. Márquez-Aguirre Estefanía Vázquez-Méndez Eduardo Padilla-Camberos Alejandro A. Canales-Aguirre unidad de Evaluation Preclinica Unidad de Biotecnologia Medica y FarmaceuticaCentro de Investigation y Asistencia en Tecnologia y Diseno del Estado de JaliscoGuadalajaraMexico Regenerative Medicine Unit Neuroscience InstituteDepartment of Neurosurgery and NeurologyIdlSSC Health Research Institute of the Hospital Clinico San CarlosMadridSpain

Studies have shown that mesenchymal stem cell-derived exosomes can enhance neural plasticity and improve cognitive *** purpose of this study was to investigate the effects of mesenchymal stem cell-derived exosomes on neurogenesis and cognitive capacity in a mouse model of Alzheimer’s ***’s disease mouse models were established by injection of beta amyloid 1?42 aggregates into dentate gyrus *** water maze and novel object recognition tests were performed to evaluate mouse cognitive deficits at 14 and 28 days after ***,neurogenesis in the subventricular zone was determined by immunofluorescence using doublecortin and PSA-NCAM *** showed that mesenchymal stem cells-derived exosomes stimulated neurogenesis in the subventricular zone and alleviated beta amyloid 1?42-induced cognitive impairment,and these effects are similar to those shown in the mesenchymal stem *** findings provide evidence to validate the possibility of developing cell-free therapeutic strategies for Alzheimer’s *** procedures and experiments were approved by Institutional animal Care and Use Committee(CICUAL)(approval *** 2016-011)on April 25,2016.

关键词： Alzheimer’s disease neurodegenerative disease cognitive impairment memory Alzheimer’s disease mouse model mesenchymal stem cell exosomes neurogenesis cognitive improvement cell-free therapy neural regeneration

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Preclinical models of idiosyncratic drug-induced liver injury(iDILI):Moving towards prediction

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Acta Pharmaceutica Sinica B 2021年第12期11卷 3685-3726页

作者： Antonio Segovia-Zafra Daniel E.Di Zeo-Sanchez Carlos Lopez-Gomez Zeus Perez-Valdes Eduardo Garcıa-Fuentes Raul J.Andrade M.Isabel Lucena Marina Villanueva-Paz unidad de Gestio´n Clı´nica de Gastroenterologı´a Servicio de Farmacologıa ClınicaInstituto de Investigacio´n Biomedica de Malaga-IBIMAHospital Universitario Virgen de la VictoriaUniversidad de MalagaMalaga 29071Spain Centro de Investigacion Biomedica en Red en el Area Tematica de Enfermedades Hepaticas y Digestivas(CIBERehd) Madrid 28029Spain Platform ISCIII de Ensayos Clınicos UICEC-IBIMAMalaga 29071Spain unidad de Gestion Clınica de Aparato Digestivo Instituto de Investigacion Biomedica de Malaga(IBIMA)Hospital Universitario Virgen de la VictoriaMalaga 29010Spai

Idiosyncratic drug-induced liver injury(iDILI) encompasses the unexpected harms that prescription and non-prescription drugs,herbal and dietary supplements can cause to the *** remains a major public health problem and a major cause of drug *** the lack of biomarkers for iDILI prediction,diagnosis and prognosis,searching new models to predict and study mechanisms of iDILI is *** of the major limitations of iDILI preclinical assessment has been the lack of correlation between the markers of hepatotoxicity in animal toxicological studies and clinically significant ***,major advances in the understanding of iDILI susceptibility and pathogenesis have come from the study of well-phenotyped iDILI ***,there are many gaps for explaining all the complexity of iDILI susceptibility and ***,there is a need to optimize preclinical human in vitro models to reduce the risk of iDILI during drug ***,the current experimental models and the future directions in iDILI modelling are thoroughly discussed,focusing on the human cellular models available to study the pathophysiological mechanisms of the disease and the most used in vivo animal iDILI *** also comment about in silico approaches and the increasing relevance of patient-derived cellular models.

关键词： Drug-induced liver injury Preclinical models Mechanisms Oxidative stress Mitochondrial damage Immune response Personalized medicine

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