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Journal of Integrative Agriculture 2015年第7期14卷 1251-1260页

作者： WU Lei JIA Yan-li WU Gang LU Chang-ming Oil crops research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

DNA sequences of fatty acid elongase 1 genes FAE1.1 （EA） and FAE1.2 （Ec） were isolated and characterized for 30 com- mercialized low erucic acid rapeseed （LEAR） cultivars in China. Four types of independent mutation leading to low erucic acid trait were found, i.e., a single-base transition （eAl）, a two-base deletion （ec2） and four-base deletion （eC4） as well as single-base transition with a four-base deletion （eA.）. Three genotypes, i.e., eA1eA1eC2eC2, eA1eA1eC4eC4 and ***4ec4 were responsible for LEA content in storage Iipids of different rapeseed cultivars. Most of the LEAR cultivars had a genotype of eA1eA1ec2ec2, which were descended from the first LEAR cultivar, Oro. Yeast expression analysis revealed that two-base-pair （AA） deletion （ec2） at the base sites of 1 422-1 423 in the C genome FAE1 gene resulted in the absence of the condensing enzyme and led to the failure to produce erucic acid. Coexpression of FAE1 and ketoacyI-CoA reductase （KCR） or enoyI-CoA reductase （ECR） was found in high erucic acid rapeseed （HEAR） but not in LEAR （eA1eA1ec2ec2oreA1eA1ec4ec4）. Moreover, KCR and ECR were still coordinately regulated in eA1eA1ec2ec2 or eA1eA1ec4ec4 genotypes, suggesting that the expression of two genes was tightly linked. In addition, specific detection methods were developed by high-resolution melting curve analysis in order to detect eA1 and ec4.

关键词： erucic acid fatty acid elongase 1 natural mutation Brassica napus L.

Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening

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horticultural Plant Journal 2020年第4期6卷 247-256页

作者： Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu Key laboratory of Biology and Genetic Improvement of horticultural crops(North China) Beijing Key Laboratory of Vegetable Germplasm ImprovementNational Engineering Research Center for VegetablesBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Watermelon fruit undergoes distinct development stages with dramatic changes during fruit *** date,the molecular mechanics of watermelon ripening remain *** and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit *** dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length ***-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening *** results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like ***069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening *** transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 ***,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit *** together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening.

关键词： watermelon ethylene response factor ERF transgenic tomato ripening

Transformation of Cabbage(Brassica oleracea ***) with Bt cry1Ba3 Gene for Control of Diamondback Moth

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Agricultural Sciences in China 2011年第11期10卷 1693-1700页

作者： YI Deng-xia CUI Lei LIU Yu-mei ZHUANG Mu ZHANG Yang-yong FANG Zhi-yuan YANG Li-mei Key laboratory of horticultural crops Genetic Improvement Ministry of Agriculture~Institute of Vegetables and Flowers Chinese Academyof Agricultural Sciences Beijing 100081 P.R.China

To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction （PCR） and Southern blot analyses confirmed that crylBa3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction （RT-PCR） demonstrated that crylBa3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by crylBa3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and CrylAc-resistant diamondback moth （DBM） larvae.

关键词： Bacillus thuringiensis crylBa3 cabbage diamondback moth resistance

Wild Brassica and Its Close Relatives in Turkey,the Genetic Treasures

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horticultural Plant Journal 2021年第2期7卷 97-107页

作者： Ali A.Donmez Zubeyde Ugurlu Aydın Xiaowu Wang Molecular Plant Systematic laboratory(MOBIS) Department of BiologyFaculty of ScienceHacettepe UniversityAnkara 06800Turkey Key laboratory of Biology and Genetic Improvement of horticultural crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China

Brassica taxa occur naturally and are also cultivated in *** to their economic importance,several cultivars have been extensively cultivated in certain regions of the *** extensive cultivation for vegetable production of the other species of the genus,Brassica juncea has very limited *** native species of Brassica are known from restricted locations in Turkey with only a few *** them,Brassica elongata is distributed all over the Central and Eastern parts of the country and it prefers unfertile soils on *** the current data about the Brassica taxa would lead to new initiatives for Brassica research dealing with both the genetic structure and the origin of the *** characters of the genera closely related to Brassica have been discussed under the relevant ***,an overview for the Turkish Brassiceae tribe,both native and cultivated,has been presented and the relevant identification keys have been supplied for updating.

关键词： Brassica Brassiceae cultivation native Turkey

Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.

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Journal of Integrative Agriculture 2015年第9期14卷 1723-1734页

作者： ZOU Xi-ling ZENG Liu LU Guang-yuan CHENG Yong XU Jin-song ZHANG Xue-kun Key laboratory of Biology and Genetic Improvement of Oil crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academy of Agricultural Sciences

RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12 h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of waterlogging tolerance beyond natural variation. A total of 2 977 genes with similar expression patterns and 17 genes with opposite expression patterns were identiifed in the transcription proifles of ZS9 and GH01. An additional 1 438 genes in ZS9 and 1 861 genes in GH01 showed strain speciifc regulation. Analysis of the overlapped genes between ZS9 and GH01 revealed that waterlogging tolerance is determined by ability to regulate genes with similar expression patterns. Moreover, differences in both gene expression proifles and abscisic acid (ABA) contents between the two varieties suggest that ABA may play some role in waterlogging tolerance. This study identiifes a subset of candidate genes for further functional analysis.

关键词： rapeseed(Brassica napus L.) waterlogging digital gene expression(DGE) roots transcriptome comparative analysis

Modulation of Sex Expression in Four Forms of Watermelon by Gibberellin, Ethephone and Silver Nitrate

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horticultural Plant Journal 2017年第3期3卷 91-100页

作者： ZHANG Jie SHI Jianting JI Gaojie ZHANG Haiying GONG Guoyi GUO Shaogui REN Yi FAN Jianguang TIAN Shouwei XU Yong National Engineering research Center for Vegetables Beijing Academy of Agriculture and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Beijing Key Laboratory of Vegetable Germplasm Improvement

There has been no systematic research on the effect of plant growth regulators and silver nitrate treatments on the control of sex expression in watermelon. In this study, we tested sex expression responses of four watermelon forms(monoecism, gynoecism, andromonoecism,and hermaphrodite) to gibberellin, ethephon and silver nitrate treatments. Results have shown that, in monoecious plants, gibberellins(GA_3)and ethephon treatments reduced the percentage of female flowers and delayed the occurrence of the first female flower, while silver nitrate induced the formation of bisexual flowers. In gynoecious plants, both ethephon and silver nitrate treatments transformed some female flowers into bisexual flowers, and treatment with ethephon resulted in a mass of abnormal flowers, while no obvious effect of treatment with GA_3 was observed. In andromonoecious plants, ethephon and GA_3 treatments delayed the occurrence of the first bisexual flower, and GA_3 reduced the percentage of bisexual flowers, while no distinct effect for silver nitrate treatment was observed. In hermaphroditic plants, ethephon treatment induced the appearance of numerous abnormal flowers, while no obvious effects for GA_3 and silver nitrate treatments were *** analyzed the transcription levels of all the expressed aminocyclopropane-1-carboxylic acid synthase(ACS) homologues in two gynoecious mutants and their wild types. We also tested the gene expression of CitACS4 which had been recognized as the andromonoecious gene in all treatments. All these results suggested that the best masculinizing treatment for breeding of the gynoecious line is silver nitrate, which repressed the expression of CitACS4 and induced many bisexual flowers for use in self-fertilization subsequently.

关键词： watermelon sex expression gibberellins(GA3) ethephon silver nitrate

Genetic dissection of root morphological traits as related to potassium use efficiency in rapeseed under two contrasting potassium levels by hydroponics

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Science China(Life Sciences) 2019年第6期62卷 746-757页

作者： Xiaoling Dun Jiaqin Shi Huiping Liu Jie Wang Xinfa Wang Hanzhong Wang Oil crops research Institute of the Chinese Academy of Agricultural Sciences/Key laboratory of Biology and Genetic Improvement of Oil crops Ministry of Agriculture

To reveal the genetic basis of potassium use efficiency(KUE) in rapeseed, root morphology(RM), biomass and KUE-related traits were measured in a recombinant inbred line population with 175 F7 lines that were subjected to high-potassium(HK) and low-potassium(LK) treatments by hydroponics. A total of 109 significant QTLs were identified to be associated with the examined traits. Sixty-one of these QTLs were integrated into nine stable QTLs. The higher heritability for RM and biomass traits and lower heritability for KUE-related traits, as well as nine stable QTLs for RM traits and only two for KUE-related traits,suggested that regulating RM traits would be more effective than selecting KUE traits directly to improve KUE by markerassisted selection. Furthermore, the integration of stable QTLs identified in the HK, LK, high-nitrogen(HN) and low-nitrogen(LN) conditions gave 10 QTL clusters. Seven of these clusters were classified into major QTLs that explained 7.4%–23.7% of the total phenotypic variation. Five of the major QTL clusters were detected under all of the treated conditions, and four clusters were specifically detected under the LK and LN conditions. These common and specific QTL clusters may be useful for the simultaneous improvement of multiple traits by marker-assisted selection.

关键词： Brassica napus L. root traits potassium efficiency QTL mapping QTL cluster

Genome-wide Identification and Expression of ARF Gene Family during Adventitious Root Development in Hot Pepper(Capsicum annuum)

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horticultural Plant Journal 2017年第4期3卷 151-164页

作者： ZHANG Huanxin CAO Ning DONG Chunjuan SHANG Qingmao Key laboratory of Biology and Genetic Improvement of horticultural crops of the Ministry of Agriculture Institute of Vegetables and FlowersChinese Academy of Agricultural Sciences

Auxin response factors(ARFs) are transcription factors that activate or repress the expression of primary/early auxin response genes by binding to auxin-responsive elements(Aux REs) in their promoter regions. The ARFs play important roles in diverse developmental *** explore the ARF gene family in hot pepper(Capsicum annuum L.), we performed a genome-wide identification and expression analysis. In this study, 19 pepper ARF genes(Ca ARFs) clustered into three phylogenetic groups(I, II, and III) were comprehensively analyzed. Conserved domain analysis showed that all Ca ARFs contained a B3 DNA-binding domain and a middle domain, but two members lacked the carboxyterminal dimerization(CTD) domain. The number of introns in Ca ARF genes ranged from 1 to 13 and the gene structure was similar among genes in the same phylogenetic group. Additionally, prediction of Ca ARFs promoter elements and putative targets for micro RNAs suggested that the regulation of Ca ARFs may occur at both transcriptional and posttranscriptional levels. Most Ca ARFs were expressed in more than one tested tissue, and most Ca ARFs were identified as being responsive to exogenous auxin. Moreover, time-course transcription profiles of Ca ARFs revealed their roles in adventitious rooting of hypocotyl cuttings from pepper seedlings. Therefore, our results will provide a foundation for better understanding the regulatory mechanisms and molecular functions of Ca ARFs in hot pepper.

关键词： hot pepper auxin response factor phylogenetic analysis gene structure promoter element adventitious root

Spatiotemporal profiles of gene activity in stamen delineate nucleo-cytoplasmic interaction in a male-sterile somatic cybrid citrus

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Horticulture research 2023年第7期10卷 138-151页

作者： Nan Jiang Meng-Qi Feng Lai-Chao Cheng Li-Hua Kuang Chao-Chao Li Zhao-Ping Yin Rong Wang Kai-Dong Xie Wen-Wu Guo Xiao-Meng Wu National Key laboratory for Germplasm Innovation&Utilization of horticultural crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan430070China Hubei Hongshan laboratory Wuhan 430070China

Cytoplasmic male sterility(CMS)has long been used to produce seedless fruits in perennial woody crops like citrus.A male-sterile somatic cybrid citrus(G1+HBP)was generated by protoplast fusion between a CMS callus parent‘Guoqing No.1’Satsuma mandarin(Citrus unshiu,G1)and a fertile mesophyll parent Hirado Buntan pummelo(Citrus grandis,HBP).To uncover the male-sterile mechanism of G1+HBP,we compared the transcriptome profiles of stamen organ and cell types at five stages between G1+HBP and HBP,including the initial stamen primordia,enlarged stamen primordia,pollen mother cells,tetrads,and microspores captured by laser *** stamen organ and cell types showed distinct gene expression profiles.A majority of genes involved in stamen development were differentially expressed,especially CgAP3.2,which was downregulated in enlarged stamen primordia and upregulated in tetrads of G1+HBP compared with *** acid-and auxin-related biological processes were enriched among the differentially expressed genes of stamen primordia,and the content of jasmonic acid biosynthesis metabolites was higher in flower buds and anthers of G1+*** contrast,the content of auxin biosynthesis metabolites was lower in G1+*** mitochondrial tricarboxylic acid cycle and oxidative phosphorylation processes were enriched among the differentially expressed genes in stamen primordia,meiocytes,and microspores,indicating the dysfunction of mitochondria in stamen organ and cell types of G1+*** together,the results indicate that malfunction of mitochondria-nuclear interaction might cause disorder in stamen development,and thus lead to male sterility in the citrus cybrid.

关键词： citrus sterile interaction

Fine-tuning the spatial allocation of phytohormones for smart canopy, smart breeding, and smart agriculture

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Science China Life Sciences 2025年第1期 309-310页

作者： Tao Zhou Lin Li National Key laboratory of Crop Genetic Improvement Huazhong Agricultural University HuBei HongShan laboratory

The dual challenges of global climate change and population growth have increased demand for higher crop yields per unit area. The innovative technology of smart agriculture aims to synergize breeding, cultivation, and production through the application of biological and informatics technologies. Historically, the “Green Revolution” introduced semidwarf varieties of certain key crops by utilizing mutations affecting gibberellin pathways, which significantly increased planting densities and yields.

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