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普通小麦白粉病成株抗性的QTL分析

作物学报 2006年第2期32卷 197-202页

作者：刘慧远 Kazuhiro Suenaga 何中虎王竹林梁闪闪马均 Michel Bernard Pierre Sourdille 夏先春中国农业科学院作物科学研究所/国家小麦改良中心 Japan International research Center for Agricultural Sciences(JIRCAS) 西北农林科技大学四川农业大学水稻研究所 INRA Amélioration et Santé des Plantes Domaine de Crouelle 234 Avenue du Brézet 63100 Clermont-Ferrand Cedex2 France

以抗白粉病的日本小麦品种Fukuho-komugi和以色列小麦Oligoculm杂交F1的DH(doubled haploid)群体107个系为材料,利用313个SSR标记和37个RFLP标记,对Fukuho-komugi和Oligoculm的白粉病成株抗性进行QTL分析。试验材料于2003—2004年度种植在北京2、003—2004和2004—2005年度种植在安阳,调查白粉病发病情况。构建了由350个位点组成的遗传连锁图,覆盖小麦21个连锁群,全长3 101 cM。采用复合区间作图法进行白粉病成株抗性QTL分析,在1A、2B4、B和7D上发现4个抗白粉病QTL,分别解释13.6%、6.6%、8.9%和12.7%的表型变异。抗白粉病基因及其紧密连锁分子标记的发掘,将为小麦抗白粉病育种的分子标记辅助选择提供理论和技术支持。

关键词：普通小麦白粉病成株抗性 QTL

A domestication-associated gene, CsLH, encodes a phytochrome B protein that regulates hypocotyl elongation in cucumber

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Molecular Horticulture 2021年第1期1卷 178-182页

作者： Bin Liu Jinyang Weng Dailu Guan Yan Zhang Qingliang Niu Enrique López-Juez Yunsong Lai Jordi Garcia-Mas Danfeng Huang School of Agriculture and Biology Shanghai Jiao Tong UniversityKey Laboratory of Urban Agriculture(South)Ministry of AgricultureDongchuan RoadShanghai 200240China Centre for research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBCampus Universitat Autònoma de Barcelona08193 BellaterraSpain College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiP.R.China Department of Biological Sciences Royal Holloway University of LondonEgham TW200EXUK Institute of Pomology&Olericulture Sichuan Agricultural UniversityChengduP.R.China

Introduction Plant height is an important agronomic trait;tall plants are prone to collapse(lodging)and are unsuitable for high-density planting(Li et al.,2020).During the Green Revolution,a multitude of genes acting as core or peripheral regulators of plant height were identified and used in breeding(Eshed and Lippman,2019);however,most were reported in cereal crop plants(Eshed and Lippman,2019)and few have been characterized in the Cucurbitaceae,which are economically important horticultural plants cultivated ***,we describe LONG HYPOCOTYL(CsLH),encoding the photoreceptor phytochrome B(PHYB),which we show has been subjected to selection during cucumber(Cucumis sativus L.)domestication.

关键词： breeding planting cultivated

Rice Blast Resistance-associated Genes Based on Different RNA-seq Resources

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Agricultural Biotechnology 2021年第6期10卷 1-6页

作者： Tao ZHOU Yalong ZHANG Dewei TAI Xiaozhong ZHANG Xueshi LIU Wei ZHANG School of Life Sciences Anhui Agricultural UniversityHefei 230036China University of Washington SeattleWA 98195U.S.A. Rice research Institute Anhui Academy of Agricultural ScienceHefei 230031China

Rice is the main food crop in Asian countries, and rice blast causes an annual loss of rice yield of 30%-35%. Cultivating rice blast resistant varieties and discovering resistance genes are important means to prevent rice blast. By analyzing the changes in rice transcriptome expression levels before and after infection by Magnaporthe oryzae of different physiological races, we found that 5 transcripts were up-regulated together after infection, Os07 t0125500-01, Os07 t0129300-00, Os07 t0126301-01 and Os07 t0124900-01 belongs to the CAP superfamily, and Os10 t0409400-01 encodes a BURP domain containing protein.

关键词： Rice blast BURP CAP RNA-seq

Zea mays L. Pollen： An Approach to Its Quality Control

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Journal of Agricultural Science and Technology(B) 2015年第8期5卷 513-522页

作者： Maria Graca Campos Norma Almaraz-Abarca] Miguel Pires Matos Nelson Mortagua Gomesl VanildaAparecida Arruda Ortrud Monika Barth Alex Silva Freitas D6bora Cristina Amancio' Ligia Bicudo Almeida-Muradian Coimbra Chemistry Center Faculty of Sciences and Technology and Faculty of Pharmacy University of Coimbra Polo 111Azinhaga de Santa Comba Coimbra 3000-548 Portugal Interdisciplinary research Centre for Integral Regional Development-Durango National Polytechnic Institute Sigma 119 20 deNoviembre 11 Durango 34220 DGO Mexico Department of Food and Experimental Nutrition Pharmaceutical Science School University of S6o Paulo Av. Prof. Lineu Prestes580 Bloco 14 CEP055800-000 Sao Paulo Brazil Instituto Oswaldo Cruz Fundaao Oswaldo Cruz Avenida Brasi14365 Rio de Janeiro 21040-900 Brazil

Zea mays L. is one of the biggest cropping systems among the sustainable development agronomy. Pollen from this crop source is unexplored and apiculture can be a good partner adding value to the product and creating new jobs helping to solve some social issues as unemployment. However, food safety is crucial, thus the aim of this study was to explore the flavonoid/phenolic profiles from Z. mays L. pollen as a fingerprint for this plant identification and also to demonstrate how the method of bee pollen samples （honeybee collected pollen） is applied. For this purpose, several sources ofZ. mays L. pollen were analyzed, including corn hybrids and genetic modified samples collected at the breeding fields. For this work, samples were taken at several years from 2000 to 2012 and collected from different countries and locations, such as Portugal, Mexico and Brazil. Results showed, for the first time, that the fingerprint （flavonoid/phenolic profile） for Z. mays L. pollen does not change over the time of sampling neither with the region of harvesting. The high performance liquid chromatography-diode array detector （HPLC/DAD） fingerprints of phenolic/flavonoid extract from Z. mays remain unchanged for all samples analyzed from different countries, hybrids and/or genetic modified plants. This is also the first study reporting these phenolic compounds not only in pollen collected directly from hybrid plants, but also in Z. mays bee pollen. The described fingerprinting method is easy, fast and accurate for the characterization of Z. mays L. pollen samples and complete microscopic analysis because it is species-specific.

关键词： Flavonoids phenols Zea mays pollen bee pollen food safely phenolic fingerprinting palynological analysis oxygenradical absorbance capacity (ORAC).

Advances in research on Drought Resistance in Rice

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Agricultural Biotechnology 2022年第5期11卷 26-33页

作者： Yueming YI Shimei WANG Xinxin CHENG Qian ZHU Yiru LI Fengbin WANG Minghua ZHANG Anhui Science And Technology University Fengyang 233100China Rice research Institute Anhui Academy of Agricultural SciencesHefei 230000China Institute of Food crops Xinjiang Academy of Agricultural SciencesUrumqi 830000China South China Agricultural University Guangzhou 510000China

Rice is the main grain crop in China. With global warming, drought and water shortage have become one of the important factors limiting rice production. Improving the drought resistance of rice can effectively alleviate the loss of rice yield caused by drought. In this paper, the research progress on rice drought resistance and its evaluation methods, mapping of QTLs for drought resistance-related traits, and mining for drought resistance genes were reviewed, and molecular breeding for drought resistance in rice was prospected.

关键词： Rice Drought resistance Methods of identification and evaluation QTL mapping

Effects of Different Sample Parts and Sterilization Methods on Contamination and Survival Rates of Shoots with Buds in Chinese Chestnut

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Agricultural Biotechnology 2023年第2期12卷 20-25页

作者： Jingjing DU Meng WANG Jing LIU Xuan WANG Haie ZHANG Jingzheng ZHANG College of Horticulture Science and Technology Hebei Normal University of Science and TechnologyChangli 066600China Engineering research Center of Chestnut Industry Technology Ministry of EducationQinhuangdao 066004China Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization Qinhuangdao 066004China Hebei Collaborative Innovation Center of Chestnut Industry Qinhuangdao 066004China

In this study,we investigated the preferable explant types and sterilization method for tissue culture in Chinese chestnut,in order to provide a technical support for the asexual reproduction of Chinese *** base,middle and apex parts of annual shoots with buds in Chinese chestnut were sampled and cut to 2-3 cm stem segments each with one bud,then sterilized orderly with different duration in 2%NaClO plus 0.1%HgCl_(2)*** results indicated that the duration of 2%NaClO for 20 min+0.1%HgCl_(2)for 15 min exerted an effective disinfection property on the middle parts of annual shoots with buds,and achieved a contamination rate under 5%and a survival rate over 90%.

关键词： Chinese chestnut Sterilization method Explant Contamination rate

基于经济型低空无人机对小麦重要产量表型性状的多生育时期获取和自动化分析

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农业大数据学报 2019年第2期1卷 19-31页

作者：丁国辉许昊温明星陈佳玮王秀娥周济南京农业大学作物表型交叉研究中心中英植物表型组学联合研究中心南京210095 江苏丘陵地区镇江农业科学研究所句容212400 南京农业大学作物遗传与种质创新国家重点实验室南京210095 英国厄尔汉姆研究中心(Earlham Institute) 诺维奇科研院(Norwich Research Park)诺维奇英国NR47UZ

多尺度表型采集技术通过多种手段获取植物图像和光谱数据,进而基于各类计算机分析算法(如,计算机视觉和机器学习)进行表型分析,得到与产量、品质和抗逆等相关的性状信息,为作物遗传育种、栽培和农业生产提供高通量、大数据的技术支撑。... 详细信息

多尺度表型采集技术通过多种手段获取植物图像和光谱数据,进而基于各类计算机分析算法(如,计算机视觉和机器学习)进行表型分析,得到与产量、品质和抗逆等相关的性状信息,为作物遗传育种、栽培和农业生产提供高通量、大数据的技术支撑。小麦作为我国重要的粮食作物,其关键产量性状的全生育期量化分析有重要意义。本文详细介绍了部分重要的小麦产量相关性状,并通过使用经济型低空无人机对不同关键生育时期中的一些共同的产量性状进行了规模化采集。然后,基于无人机获取的可见光图像,通过第三方专业软件Pix4D完成了全试验田的拼接和三维点云重建,并通过自主开发的性状分析算法对一些重要产量性状和植被指数等完成了自动化分析。同时,针对18个不同的小麦基因型完成了关键生育时期的株高、植被指数、叶面积指数的提取。通过实例验证了基于经济型低空无人机开展小麦产量性状采集的有效方法和高通量分析技术。本研究对降低田间作物表型研究的门槛,促进我国各研究团队采用标准化表型数据采集,统一作物表型数据规范,以及推广使用开源软件自主开发自动化分析技术平台有重要意义。

关键词：小麦产量性状无人机表型技术高通量性状分析图像处理表型植物表型组学

Near‐infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia

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