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Temperate Stutzerimonas Phage Encoding Toxin-Antitoxin System Genes Represents a Novel Genus

Journal of Ocean University of China 2024年第4期23卷 1087-1101页

作者： ZHANG Hong GUO Ruizhe GAO Chen ZHENG Kaiyang XIONG Yao DONG Yue LIU Yundan WANG Ziyue CHEN Ying WANG Hongmin SHAO Hongbing SUNG Yeong Yik MOK Wen Jye WONG Li Lian MCMINN Andrew WANG Min LIANG Yantao College of Marine Life Sciences Institute of Evolution and Marine BiodiversityMOE Laboratory of Evolution and Marine BiodiversityFrontiers Science Center for Deep Ocean Multispheres and Earth SystemCenter for Ocean Carbon NeutralityOcean University of ChinaQingdao 266003China umt-OUC Joint Center for Marine Studies Qingdao 266003China Institute of Marine Biotechnology Universiti Malaysia Terengganu21030 Kuala TerengganuMalaysia Institute for Marine and Antarctic Studies University of TasmaniaHobartTAS 7001Australia Haide College Ocean University of ChinaQingdao 266100China The Affiliated Hospital of Qingdao University Qingdao 266001China

Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological ***,research on its phages is currently *** this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri *** dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading *** phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate *** addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regulating host growth and *** phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank ***,it has a connection with enigmatic,uncultured ***,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 *** significant finding has resulted in the identification of a novel viral genus known as Metabovirus.

关键词： Stutzerimonas vB_SstM-PG1 Metabovirus temperate phage genomic and phylogenetic analysis

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DeepSVDNet:A Deep Learning-Based Approach for Detecting and Classifying Vision-Threatening Diabetic Retinopathy in Retinal Fundus Images

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Computer Systems Science & Engineering 2024年第2期48卷 511-528页

作者： Anas Bilal Azhar Imran Talha Imtiaz Baig Xiaowen Liu Haixia Long Abdulkareem Alzahrani Muhammad Shafiq College of Information Science and Technology Hainan Normal UniversityHaikou571158China Department of Creative Technologies Air UniversityIslamabadPakistan School of Life Science and Technology University of Electronic Science and Technology of China UESTCChengduChina School of Science and Technology University of Management and Technology UMTLahorePunjabPakistan Faculty of Computer Science&Information Technology Al Baha UniversityAl BahaSaudi Arabia School of Information Engineering Qujing Normal UniversityQujingChina

Artificial Intelligence(AI)is being increasingly used for diagnosing Vision-Threatening Diabetic Retinopathy(VTDR),which is a leading cause of visual impairment and blindness ***,previous automated VTDR detection methods have mainly relied on manual feature extraction and classification,leading to *** paper proposes a novel VTDR detection and classification model that combines different models through majority *** proposed methodology involves preprocessing,data augmentation,feature extraction,and classification *** use a hybrid convolutional neural network-singular value decomposition(CNN-SVD)model for feature extraction and selection and an improved SVM-RBF with a Decision Tree(DT)and K-Nearest Neighbor(KNN)for *** tested our model on the IDRiD dataset and achieved an accuracy of 98.06%,a sensitivity of 83.67%,and a specificity of 100%for DR detection and evaluation tests,*** proposed approach outperforms baseline techniques and provides a more robust and accurate method for VTDR detection.

关键词： Diabetic retinopathy(DR) fundus images(FIs) support vector machine(SVM) medical image analysis convolutional neural networks(CNN) singular value decomposition(SVD) classification

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Epidemiology of Polio virus infection in Pakistan and possible risk factors for its transmission

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Asian Pacific Journal of Tropical Medicine 2016年第11期9卷 1022-1025页

作者： Mahvish Kabir Muhammad Sohail Afzal Department of Chemistry School of Science.University of Management and Technology(UMT)

End Polio Pakistan program still has to overcome many hurdles;unfortunately on 8th February2016 first polio case of the year has surfaced in *** seems that battle against polio demands little bit more conviction and *** has set a goal of polio eradication in Pakistan till 2018,in order to evaluate the success of this target;polio eradication campaign in Pakistan has been analyzed in different *** analysis indicated that major obstacles in eradication are low literacy rate,poor health infrastructure,lack of planning,natural disaster,economic crisis,counter insurgencies and almost no protection for polio health *** has allocated new funds to tackle this problem,now there is a need to spend this money more effectively with proper planning and honest deployment of funds.

关键词： Eradication Malnutrition Counter insurgencies Polio worker Hurdles in polio Pakistan

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Are efforts up to the mark? A cirrhotic state and knowledge about HCV prevalence in general population of Pakistan

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Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 597-598页

作者： Muhammad Sohail Afzal Department of Chemistry School of Science University of Management and Technology (UMT)

Pakistan is declared a cirrhotic state by WHO because of high prevalence of Hepatitis C virus(HCV). It is proposed that there are more than 10 million infected individuals in Pakistan. Keeping in mind the scenario, one should expect to have proper surveillance system for HCV monitoring across the country not only for high risk groups but also for general population as well. Monitoring the general population is of considerable help to identify the hot spots for infection spread and will help in designing effective strategies. In this study the current available data from last 6 years is analyzed. The results showed that the range of HCV seroprevalence in general population in Pakistan varied from 3.13%-23.83%. Similarly year wise HCV prevalence showed great variation and the prevalence was 9.57%, 6.04%, 7.46%, 4.9% and 11.04% in 2010, 2011, 2012, 2013 and 2014 respectively. The current analysis declared that available data is not sufficient and is not presenting the original picture of the situation. On these basis, a proper surveillance system is proposed across the country to get an actual picture of the infection for better management in future and massive community education programmes should be warranted in identified hot spots across the country.

关键词： HCV General population Prevalence Community education Pakistan

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Role of Nd_2O_3 nanoparticles addition on microstructural and superconducting properties of YBa_2Cu_3O_(7–δ) ceramics

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Journal of Rare Earths 2016年第9期34卷 895-900页

作者： Aima Ramli Abdul Halim Shaari Hussein Baqiah Chen Soo Kean Mohd Mustafa Awang Kechik Zainal Abidin Talib Superconductivity and Thin Films Laboratory Department of Physics Universiti Putra Malaysia 43400 Serdang Selangor Malaysia Department of Physics School of Fundamental Science Universiti Malaysia Terengganu (UMT) 21030 Kuala Terengganu Terengganu Malaysia

The effects of Nd2O3 nanoparticles addition on microstructure, transport and AC susceptibility properties of YBa2Cu3O7–δ（Y123） superconductors were systematically investigated using X-ray diffraction （XRD）, scanning electron micrograph （SEM）, four point probe measurement and AC spectrometer. It was found that the added samples were predominant by Y-123 phase beside small amount of Y-211 and unreacted Nd2O3secondary phases. All added samples preserved the orthorhombic structure similar to the pure sample and no orthorhombic-to-tetragonal transition occurred. The samples became more porous and their grain size significantly de-creased with addition of Nd2O3. The addition of nano-Nd2O3 disturbed the grain growth of Y123, thus resulting in the degradation of superconducting properties of the samples. The superconducting transition temperature （Tc onset） of samples decreased from 92 K for x=0.0 to 78 K forx=1.0 wt.%, which could be attributable to oxygen vacancy disorder. From AC susceptibility result, the inter- and intra-granular loss peaks became wider and broader with increase of Nd2O3 addition due to the weakening of grains coupling. On the other hand, the inter-granular critical current density,Jcm, was found to increase with Nd2O3 addition and had the highest value at x=0.6, confirming that Nd2O3 nanoparticles acted as pinning centers in Y123 matrix.

关键词： YBCO critical temperature co-precipitation AC susceptibility critical current density rare earths

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Household Income and Expenditure Relationships： A Simultaneous Equation Approach

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Chinese Business Review 2011年第6期10卷 395-405页

作者： Nik Hashim Nik Mustapha Noorhaslinda Kulub Abd. Rashid Aslina Nasir University of Malaysia Terengganu （umt） Kuala Terengganu Malaysia

Income and expenditure are highlighted in this study as they currently affect the quality of life of the Malaysian society. In the Ninth Malaysia Plan （2006-2010） poverty eradication was emphasized as one of the socio-economic targets to uplift those poor households from the fast moving modern sectors of the economy. The primary objective of this paper is therefore to identify income and expenditure relationships among the households of the east coast of Peninsular Malaysia during the current economic downturn. Attempts to analyze household consumption as a share of total income and to identify factors contributing to household income and expenditure have become a topic of interest in literature. A substantial portion of household income devoted to productive loans characterizes the majority of Malaysians as the economy progresses to a more advance stage. A significant and strong relationship between income and types of household expenditure is explored from the socio-economic perspectives. Using primary data a system of simultaneous equations, namely the two stage least squares （2SLS） was applied. This paper identifies crucial variables associated with the income and expenditure. Some of these variables showing high correlation with the total expenditure are the housing loan, automobile loan and educational expenditure.

关键词： expenditure income simultaneous two-stage equation method

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Correction: Genomic diversity and ecological distribution of marine Pseudoalteromonas phages

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Marine Life Science & Technology 2023年第2期5卷 286-288页

作者： Kaiyang Zheng Yue Dong Yantao Liang Yundan Liu Xinran Zhang Wenjing Zhang Ziyue Wang Hongbing Shao Yeong Yik Sung Wen Jye Mok Li Lian Wong Andrew McMinn Min Wang College of Marine Life Sciences Institute of Evolution and Marine Biodiversityand Frontiers Science Center for Deep Ocean Multispheres and Earth SystemOcean University of ChinaQingdao 266100China umt-OUC Joint Center for Marine Studies Qingdao 266003China Institute of Marine Biotechnology Universiti Malaysia Terengganu(UMT)21030 Kuala NerusMalaysia Institute for Marine and Antarctic Studies University of TasmaniaHobartAustralia Haide College Ocean University of ChinaQingdao 266100China The Afliated Hospital of Qingdao University Qingdao 266000China

In this article the graphics relating to Figs.2 and 3 captions had been interchanged;the fgure(s)should have appeared as shown *** original article has been *** Access This article is licensed under a Creative Commons... 详细信息

关键词： Open Creative Access License

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Why more research needs to be done on echinococcosis in Pakistan

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Infectious Diseases of Poverty 2017年第1期6卷 786-790页

作者： Haroon Ahmed Shahzad Ali Muhammad Sohail Afzal Abid Ali Khan Hamid Raza Zaheer Hussain Shah Sami Simsek Department of Biosciences COMSATS Institute of Information Technology(CIIT)Park RoadChak ShahzadIslamabadPakistan Department of Parasitology Faculty of Veterinary MedicineUniversity of Firat23119ElazigTurkey Department of Wildlife and Ecology University of Veterinary and Animal SciencesLahorePakistan Department of Chemistry School of ScienceUniversity of Management and Technology(UMT)LahorePakistan School of Science University of Management and Technology(UMT)LahorePakistan

Background:Echinococcosis has a worldwide geographical distribution with endemic foci on every inhabited *** to the frequent outbreaks in different parts of Pakistan in the recent past,echinococcosis is being described as a neglected tropical disease and is considered one of the most neglected parasitic diseases in the *** endemic regions,predominantly settings with limited resources,there are high numbers of echinococcosis patients,as these communities do not have access to appropriate *** Pakistan,there are limited reports on *** disease is prevalent in human and livestock,but this has not been sufficiently explored *** is an agricultural country and due to the disease’s zoonotic mode of transmission,there is a dire need of future research on *** present paper is an effort to highlight the importance of echinococcosis in ***:There is a dire need for future research on echinococcosis in Pakistan as very few investigations had been carried out on this topic thus *** prevalence of the disease in neighbouring countries highlights that Pakistan might be at severe risk of this zoonotic infection and further supports the need for more *** Pakistan,the majority of the population lives in rural areas with limited acess to proper hygienic/sanitary *** conditions favour the outbreak of diseases such as *** limited available data could result in higher outbreaks in the future,and thus cause the already weak healthcare system to *** country has a meagre annual budget for health,which is being spent on known infections such as polio,dengue fever and hepatic viral infections.A proper surveillance system for echinococcosis is required across the country as treatment is usually expensive,complicated and may require extensive surgery and/or prolonged drug *** of new/novel drugs and other treatment modalities receives very little,if any,***

关键词： Pakistan Echinococcosis Neglected tropical diseases Research

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Scoring Cercospora Leaf Spot on Sugar Beet:Comparison of UGV and UAV Phenotyping Systems

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Plant Phenomics 2020年第1期2卷 225-242页

作者： S.Jay A.Comar R.Benicio J.Beauvois D.Dutartre G.Daubige W.Li J.Labrosse S.Thomas N.Henry M.Weiss F.Baret INRAE UMR 114 EMMAHUMT CAPTEF-84914 AvignonFrance HIPHEN SAS 84000 AvignonFrance ARVALIS-Institut du végétal 84000 AvignonFrance Florimond Desprez 59242 Capelle-en-PévèleFrance

Selection of sugar beet(Beta vulgaris L.)cultivars that are resistant to Cercospora Leaf Spot(CLS)disease is critical to increase *** selection requires an automatic,fast,and objective method to assess CLS severity on thousands of cultivars in the *** this purpose,we compare the use of submillimeter scale RGB imagery acquired from an Unmanned Ground Vehicle(UGV)under active illumination and centimeter scale multispectral imagery acquired from an Unmanned Aerial Vehicle(UAV)under passive *** variables are extracted from the images(spot density and spot size for UGV,green fraction for UGV and UAV)and related to visual scores assessed by an *** show that spot density and green fraction are critical variables to assess low and high CLS severities,respectively,which emphasizes the importance of having submillimeter images to early detect CLS in field *** sensitivity to CLS can then be accurately retrieved based on time integrals of UGV-and UAV-derived *** UGV shows the best estimation performance,UAV can show accurate estimates of cultivar sensitivity if the data are properly *** and limitations of UGV,UAV,and visual scoring methods are finally discussed in the perspective of high-throughput phenotyping.

关键词： illumination millimeter critical

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Outflow of traffic from the national capital Kuala Lumpur to the north,south and east coast highways using flow,speed and density relationships

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Journal of Traffic and Transportation Engineering(English Edition) 2016年第6期3卷 540-548页

作者： Nik Hashim Nik Mustapha Nik Nur Wahidah Nik Hashim School of Sodal and Economic Development University of Malaysia Terengganu(UMT)21030 Kuala TerengganuMalaysia Mechatronics Department School of EngineeringInternational Islamic University Malaysia(IIUM)50728 Kuala LumpurMalaysia

The functional relationships between flow （veh/km）, density （veh/h） and speed （kin/h） in traffic congestion have a long history of research. However, their findings and techniques persist to be relevant to this day. The analysis is pertinent, particularly in finding the best fit for the three major highways in Malaysia, namely the KL-Karak Highway, KL-Seremban Highway and KL-Ipoh Highway. The trans-logarithm function of density-speed model was compared to the classical models of Greenshields, Greenberg, Underwood and Drake et al. using data provided by the Transport Statistics Malaysia 2014. The results of regression analysis revealed that the Greenshields and Greenberg models were statistically significant. The trans-logarithm function was also tested and the results were nonetheless without exception. Its usefulness in addition to statistical significance related to the derived economic concepts of maximum speed and the related number of vehicles, flow and density and the limits of free speed were relevant in comparing the individual levels of traffic congestion between highways. For instance, KL-Karak Highway was least congested compared to KL-Seremban Highway and KL-Ipoh Highway. Their maximum speeds, based on three lanes carriage capacity of one direction, were 33.4 km/h for KL-Karak, 15.9 km/h for KL-Seremban, and 21.1 km/h for KL-Ipoh. Their corresponding flows were approximated at 1080.9 veh/h, 1555.4 veh/h, and 1436.6 veh/h.

关键词： Traffic congestion Maximum flow-speed Density-speed Trans-logarithm function

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