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Development of a Fast and Facile Analytical Approach to Quantify Radiometabolites in Human Plasma Samples Using Ultra High Performance Liquid Chromatography

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American Journal of Analytical Chemistry 2019年第5期10卷 185-201页

作者： Cé cile Malherbe Rudy Bidault Claude Netter Denis Guilloteau Johnny Vercouillie Nicolas Arlicot umr 1253 iBrain Universit&#233 de Tours Inserm Tours France CHRU de Tours Unit&#233 de Radiopharmacie Tours France Thermo Fisher Scientific Waltham MA USA CHRU de Tours Service de M&#233 decine Nuclé aire in Vitro Tours France INSERM CIC 1415 University Hospital Tours France

Introduction: Conventional metabolite analyses often require manual sample preparation, generating variability of measurements. This study describes a new method to quantify radiometabolites in blood, combining ultra high performance liquid chromatography (UHPLC) and turbulent flow chromatography, an alternative fully automated process allowing analyte’s extraction. Methods: A new radiotracer for dopamine transporter imaging, namely LBT-999, was used to demonstrate the method’s robustness. Matrix effect, Turboflow column loading, linearity, specificity and precision were evaluated with in vitro samples of LBT-999 in human plasma. Radiodetector sensitivity and preliminary evaluation were respectively determined by analysis of calibrated samples of [18F]LBT-999 and blood samples from 4 healthy subjects injected with [18F]LBT-999, withdrawn at 5, 15, 30 and 45 min pi. Results: With three sequential loadings (3 × 100 μL) of the Turboflow column, mean coefficients of variation were 1%, below 2%, 2% and 30.9% for matrix effect, specificity, repeatability and intermediate precision, respectively. Correlation coefficients for linearity were superior to 0.97. Limits of detection and quantification of the radiodetector were fixed at 3 and 9 c/s. Retention times for [18F]LBT-999 and the two radiometabolites detected by radio-UHPLC were 6.5, 4.8 and 9.6 min. Forty-five min after the injection, parent fraction was still predominant with 57.8% ± 25% of the total radioactivity. Conclusions: An innovative approach, allying UHPLC and Turboflow column, was developed and its sensitivity, linearity, specificity and repeatability validated. Preliminary results of the clinical trial are in accordance with literature data, demonstrating its efficiency in radiometabolites quantification.

关键词： Radiometabolite Ultra High Performance Liquid Chromatography Turboflow Method Validation [18F]LBT-999

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The POM-DOM piezophilic microorganism continuum(PDPMC)—The role of piezophilic microorganisms in the global ocean carbon cycle

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Science China Earth Sciences 2015年第1期58卷 106-115页

作者： FANG JiaSong ZHANG Li LI Jiang Tao Chiaki KATO Christian TAMBURINI ZHANG YuZhong DANG HongYue WANG GuangYi WANG FengPing State Key Laboratory of Marine Geology School of Ocean and Earth ScienceTongji University Department of Natural Sciences Hawaii Pacific University State Key Laboratory of Geological Processes and Mineral Resources Faculty of Earth SciencesChina University of Geosciences Institute of Biogeoscience Japan Agency for Marine-Earth Science and Technology (JAMSTEC) 2-15 Natsushima-cho Aix Marseille Université CNRS Université de Toulon IRD State Key Laboratory of Microbial Technology Shandong University State Key Laboratory of Marine Environmental Sciences Institute of Marine Microbes and Ecospheres Xiamen University Center for Marine Ecology and Environmental Research Tianjin University State Key Laboratory of Microbial Metabolism and State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University

The deep ocean piezosphere accounts for a significant part of the global ocean,hosts active and diverse microbial communities which probably play a more important role than hitherto recognized in the global ocean carbon *** conventional biological pump concept and the recently proposed microbial carbon pump mechanism provide a foundation for our understanding of the role of microorganisms in cycling of carbon in the ***,there are significant gaps in our knowledge and a lack of mechanistic understanding of the processes of microbially-mediated production,transformation,degradation,and export of marine dissolved and particulate organic matter(DOM and POM)in the deep ocean and the ecological *** we propose the POM-DOM piezophilic microorganism continuum(PDPMC)conceptual model,to address these important biogeochemical processes in the deep *** propose that piezophilic microorganisms(bacteria and archaea)play a pivotal role in deep ocean carbon cycle where microbial production of exoenzymes,enzymatic breakdown of DOM and transformation of POM fuels the rapid cycling of marine organic matter,and serve as the primary driver for carbon cycle in the deep ocean.

关键词：微生物群落全球海洋碳循环生物地球化学过程颗粒有机物微生物生产微生物碳降解过程

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<i>Imperata yellow mottle virus</i>: An Emerging Threat to Maize, Sorghum and Pearl Millet in Burkina Faso

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Agricultural Sciences 2017年第5期8卷 397-408页

作者： Moustapha Koala Valentin Stanislas Edgar Traoré Drissa Sérémé Bouma James Neya Christophe Brugidou Nicolas Barro Oumar Traoré Laboratoire Mixte Internationale (LMI) Patho-Bios Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Universite Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso umr IPME (UM IRD CIRAD) Institut de Recherche pour le Développement IRD Montpellier France

Imperata yellow mottle caused by Imperata yellow mottle virus (IYMV) of the genus Sobemovirus was first characterized on Imperata cylindrical and Zea mays in Burkina Faso. The disease has been reported in several localities of the country but its ecology and epidemiology are poorly known. In particular, only I. cylindrical and maize have been reported within IYMV host range. The aim of this study was to investigate the experimental host range of the virus. Mechanical inoculation of a mixture of four IYMV isolates to 18 plant species, including four crops (maize, rice, sorghum and pearl millet) and 14 wild grasses showed clear mottle symptoms in maize, sorghum and pearl millet and two wild grass species (Setaria verticillata and Rottboellia exaltata). Symptom development was confirmed by Enzyme-linked immunosorbent assay and reverse transcription–polymerase chain reaction (RT-PCR). Infection of crop species by IYMV depended on cultivars tested. Therefore, proportions of positive cultivars were 16/36 in maize, 4/10 in sorghum and 4/9 in pearl millet, respectively. Studies on virus-host interactions using individual virus isolates showed two pathogenic patterns. Three out of the four isolates tested infected all plant species and cultivars. In contrast, the fourth IYMV isolate could infect only one maize cultivar. These results expand the previously known host range of IYMV from two to five species, indicating a narrow host range. Among the new characterized host species, sorghum and pearl millet are important cereal crops. Therefore, Imperata yellow motte disease is a potential threat for the cereal crop production and its ecology and epidemiology should be thoroughly investigated.

关键词： IYMV Sobemovirus Host Range Virus-Host Interactions

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Studies of laser-plasma interaction physics with low-density targets for direct-drive inertial confinement fusion on the Shenguang III prototype

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Matter and Radiation at Extremes 2021年第2期6卷 54-66页

作者： V.T.Tikhonchuk T.Gong N.Jourdain O.Renner F.P.Condamine K.Q.Pan W.Nazarov L.Hudec J.Limpouch R.Liska M.Krus F.Wang D.Yang S.W.Li Z.C.Li Z.Y.Guan Y.G.Liu T.Xu X.S.Peng X.M.Liu Y.L.Li J.Li T.M.Song J.M.Yang S.E.Jiang B.H.Zhang W.Y.Huo G.Ren Y.H.Chen W.Zheng Y.K.Ding K.Lan S.Weber ELI-Beamlines Institute of PhysicsCzech Academy of Sciences25241 Dolni BrezanyCzech Republic Centre Lasers Intenses et Applications University of Bordeaux-CNRS-CEA33405 TalenceFrance Laser Fusion Research Center China Academy of Engineering PhysicsMianyang 621900China Institute of Plasma Physics Czech Academy of Sciences18200 PragueCzech Republic Independent Foam Target Supplier CarnoustieDD76DPUnited Kingdom Faculty of Nuclear Sciences and Physical Engineering Czech Technical University in Prague11519 PragueCzech Republic Institute of Applied Physics and Computational Mathematics Beijing 100088China Center for Applied Physics and Technology Peking UniversityBeijing 100871China School of Science Xi’an Jiaotong UniversityXi’an 710049China

The physics of laser-plasma interaction is studied on the Shenguang III prototype laser facility under conditions relevant to inertial confinement fusion designs.A sub-millimeter-size underdense hot plasma is created by ionization of a low-density plastic foam by four high-energy(3.2 kJ)laser *** interaction beam is fired with a delay permitting evaluation of the excitation of parametric instabilities at different stages of plasma *** diagnostics are used for plasma characterization,scattered radiation,and accelerated *** experimental results are analyzed with radiation hydrodynamic simulations that take account of foam ionization and *** measured level of stimulated Raman scattering is almost one order of magnitude larger than that measured in experiments with gasbags and hohlraums on the same installation,possibly because of a greater plasma *** amplification is achieved in high-intensity speckles,indicating the importance of implementing laser temporal smoothing techniques with a large bandwidth for controlling laser propagation and absorption.

关键词： density. interaction scattered

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Diverse bacterial populations of PM_(2.5) in urban and suburb Shanghai,China

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Frontiers of Environmental Science & Engineering 2021年第3期15卷 77-86页

作者： Caihong Xu Jianmin Chen Zhikai Wang Hui Chen Hao Feng Lujun Wang Yuning Xie Zhenzhen Wang Xingnan Ye Haidong Kan Zhuohui Zhao Abdelwahid Mellouki Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP^(3)) Department of Environmental Science&EngineeringFudan Tyndall CentreInstitute of Atmospheric SciencesFudan UniversityShanghai 200438China Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Institute of Eco-Chongming(IEC) Yangtze River Delta Estuary Wetland StationSchool of Geographic SciencesEast China Normal UniversityShanghai 200062China School of Public Health Key Laboratory of Public Health Safety(Ministry of Education)NHC Key Laboratory of Health Technology AssessmentFudan UniversityShanghai 200032China Institut de Combustion AerothermiqueReactivite et EnvironnementCNRS45071 Orleans Cedex 02France

Airborne bacteria play key roles in terrestrial and marine ecosystems and human health,yet our understanding of bacterial communities and their response to the environmental variables lags significantly behind that of other components of PM_(2.5).Here,atmospheric fine particles obtained from urban and suburb Shanghai were analyzed by using the qPCR and Illumina Miseq *** bacteria with an average concentration of 2.12× 10^(3 )cells/m^(3),were dominated by Sphingomonas,Curvibacter,Acinetobacter,Bradyrhizobium,Methylobacterium,Halomonas,Aliihoeflea,and Phyllobacterium,which were related to the nitrogen,carbon,sulfur cycling and human health *** results provide a global survey of bacterial community across urban,suburb,and high-altitude *** Shanghai(China),urban PM2.5 harbour more diverse and dynamic bacterial populations than that in the *** structural equation model explained about 27%,41%,and 20%^78%of the variance found in bacteria diversity,concentration,and discrepant genera among urban and suburb *** work furthered the knowledge of diverse bacteria in a coastal Megacity in the Yangtze river delta and emphasized the potential impact of environmental variables on bacterial community structure.

关键词： PM_(2.5) Bacteria 16SrRNA SEM analysis Shanghai City

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Disentangling land model uncertainty via Matrix-based Ensemble Model Inter-comparison Platform(MEMIP)

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Ecological Processes 2022年第1期11卷 222-237页

作者： Cuijuan Liao Yizhao Chen Jingmeng Wang Yishuang Liang Yansong Huang Zhongyi Lin Xingjie Lu Yuanyuan Huang Feng Tao Danica Lombardozzi Almut Arneth Daniel SGoll Atul Jain Stephen Sitch Yanluan Lin Wei Xue Xiaomeng Huang Yiqi Luo Department of Earth System Science Ministry of Education Key Laboratory for Earth System ModelingInstitute for Global Change StudiesTsinghua UniversityBeijingChina Joint Innovation Center for Modern Forestry Studies College of Biology and the EnvironmentNanjing Forestry UniversityNanjingChina Department of Geography University of CambridgeCambridgeUK School of Life Science Nanjing UniversityNanjingChina Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Guangdong Province Key Laboratory for Climate Change and Natural Disaster StudiesSchool of Atmospheric SciencesSun Yat-Sen UniversityGuangzhouChina CSIRO Oceans and Atmosphere Aspendale 3195Australia Terrestrial Sciences Section Climate and Global DynamicsNational Center for Atmospheric ResearchBoulderCO 80305USA Karlsruhe Institute of Technology Institute of Meteorology and Climate Research/Atmospheric Environmental ResearchGarmisch-PartenkirchenGermany UniversitéParis Saclay CEA-CNRS-UVSQLSCE/IPSLGif sur YvetteFrance Department of Atmospheric Sciences University of IllinoisUrbanaILUSA College of Life and Environmental Sciences University of ExeterExeterUK Department of Computer Science and Technology Tsinghua UniversityBeijingChina Center for Ecosystem Science and Society Department of Biological SciencesNorthern Arizona UniversityFlagstaffAZUSA

Background:Large uncertainty in modeling land carbon(C)uptake heavily impedes the accurate prediction of the global C *** the uncertainty sources among models is crucial for model improvement yet has been difficult due to multiple feedbacks within Earth System Models(ESMs).Here we present a Matrix-based Ensemble Model Inter-comparison Platform(MEMIP)under a unified model traceability framework to evaluate multiple soil organic carbon(SOC)*** the MEMIP,we analyzed how the vertically resolved soil biogeochemistry structure influences SOC prediction in two soil organic matter(SOM)*** comparing the model outputs from the C-only and CN modes,the SOC differences contributed by individual processes and N feedback between vegetation and soil were explicitly ***:Results showed that the multi-layer models with a vertically resolved structure predicted significantly higher SOC than the single layer models over the historical simulation(1900–2000).The SOC difference between the multi-layer models was remarkably higher than between the single-layer *** analysis indicated that over 80%of the SOC increase in the multi-layer models was contributed by the incorporation of depth-related processes,while SOC differences were similarly contributed by the processes and N feedback between models with the same soil depth ***:The output suggested that feedback is a non-negligible contributor to the inter-model difference of SOC prediction,especially between models with similar process *** analysis with TRENDY v7 and more extensive MEMIP outputs illustrated the potential important role of multi-layer structure to enlarge the current ensemble spread and the necessity of more detail model decomposition to fully disentangle inter-model *** stressed the importance of analyzing ensemble outputs from the fundamental model structures,and holding a holistic view in understanding the en

关键词： Soil organic carbon Inter-model comparison Uncertainty analysis Carbon-nitrogen coupling Vertical resolved soil biogeochemistry structure

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Soil Carbon Stocks under Amazonian Forest:Distribution in the Soil Fractions and Vulnerability to Emission

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Open Journal of Forestry 2017年第2期7卷 121-142页

作者： Jean Dalmo de Oliveira Marques Flávio Jesus Luizao Wenceslau Geraldes Teixeira Euler Melo Nogueira Philip Martin Fearnside Max Sarrazin Federal Institute of Education Science and Technology of the Amazonas(IFAM)Campus Center of ManausManausBrazil National Institute for Research in Amazonia(INPA) ManausBrazil National Center for Soil Research Brazilian Agricultural Research Corporation(EMBRAPA)Rio de JaneiroBrazil IRD UMR BIOEMCORoute de MontaboCayenne CedexFrench Guiana

Transformations of natural ecosystems in tropical regions, which are usually covered by high-biomass forests, contribute to increased atmospheric CO2. Much of the carbon in forest ecosystems is stored in the soil. This study estimates soil carbon stock in a dense forest in central Amazonia from sets of soil samples collected in three topographic positions (plateau, slope and valley bottom). Soil organic matter (SOM) was fractionated by density and particle size, thus obtaining the free light fraction (FLF), intra-aggregated light fraction (IALF), sand fraction (F-sand), clay fraction (F-clay) and silt fraction (F-silt). Soil organic carbon (SOC) stocks on the plateaus (Oxisol), slopes (Ultisol) and valley bottoms (Spodosol) were 98.4 ± 7.8 Mg·ha-1, 72.6 ± 5.4 Mg·ha-1 and 81.4 ± 8.9 Mg·ha-1, respectively. Distribution of carbon in soil fractions was: 112.6 ± 15 Mg·ha-1 (FLF), 2.5 ± 0 Mg·ha-1 (ILAF), 40.5 ± 1.5 Mg·ha-1 (F-silt), 68.5 ± 4.2 Mg·ha-1 (F-clay) and 28.3 ± 1.4 Mg·ha-1 (F-sand), totaling 252.4 ± 22.1 Mg·ha-1 of carbon. Carbon is largely in labile form and near the soil surface, making it liable to release from deforestation or from climate change. Spodosols are more susceptible to soil carbon losses, demonstrating the need to preserve forested areas close to Amazonian rivers and streams.

关键词： Fractionation of Carbon Carbon Stocks Soil Physics Global Warming Amazon Rainforest Brazil

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激光焊接Ti-6Al-4V钛合金塑性行为的原位SEM/EBSD分析（英文）

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中国材料进展 2019年第3期38卷 279-285页

作者：党宁刘玲玉 LAVOGIEZ Cyril MAIRE Eric 马朝利周廉北京航空航天大学材料科学与工程学院北京100191 INSA de Lyon Mateis UMR 5510 宝钛集团有限公司陕西宝鸡721014 西北有色金属研究院陕西西安710016

选用原位SEM/EBSD显微术分析研究了Ti-6Al-4V钛合金的焊接区域的变形行为。研究发现,由于焊接时高的焊接温度(在β相变点之上)和急剧的冷却速率,导致β→α'马氏体相变的发生。SEM观察发现,在焊缝区只存在有针状的α'马氏体形貌,而在焊... 详细信息

选用原位SEM/EBSD显微术分析研究了Ti-6Al-4V钛合金的焊接区域的变形行为。研究发现,由于焊接时高的焊接温度(在β相变点之上)和急剧的冷却速率,导致β→α'马氏体相变的发生。SEM观察发现,在焊缝区只存在有针状的α'马氏体形貌,而在焊接第一热影响区和第二热影响区,针状α'相逐渐发生球化,形成了明显的显微组织梯度过渡。在对焊接区域进行SEM/EBSD下的原位拉伸实验后发现,该区域的塑性变形可划分为两个阶段。变形的第一阶段,滑移变形或孪生变形均有可能发生,这取决于晶粒的初始晶体学取向是否可通过旋转来匹配//TA(拉伸方向);而在变形的第二阶段,只发生滑移变形。

关键词：晶体学取向原位SEM 塑性变形激光焊接 Ti-6Al-4V钛合金

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广西黄曲霉、烟曲霉遗传多态性分析

广西黄曲霉、烟曲霉遗传多态性分析

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第四届全国人畜共患病学术研讨会2014年中国狂犬病年...

作者：王冬英黄维义 Jacques GUILLOT 广西大学动物科学技术学院 Ecole Nationale Vétérinaire d’Alfort Université Paris Est CréteilAgence Nationale de Sécurité SanitaireUMR Bipar Dynamyc Université Paris Est

＜正＞黄曲霉（Aspergillus flavus）、烟曲霉（Aspergillus fumigatus）是腐生性的丝状真菌,是动、植物条件致病菌,其中黄曲霉还是黄曲霉毒素的主要产生菌。黄曲霉、烟曲霉广泛分布于自然界,呈世界性分布,尤其是热带、亚热带地区。据...

＜正＞黄曲霉（Aspergillus flavus）、烟曲霉（Aspergillus fumigatus）是腐生性的丝状真菌,是动、植物条件致病菌,其中黄曲霉还是黄曲霉毒素的主要产生菌。黄曲霉、烟曲霉广泛分布于自然界,呈世界性分布,尤其是热带、亚热带地区。据报道广西地区自然界中产毒黄曲霉多,玉米中产毒菌株的检出率为59.3%,同时广西也是原发性肝癌的高发区,而黄曲霉毒素是已被证实的危险因素。黄曲霉、烟曲霉是引起人曲霉病的最常见的致病种,因此掌握黄曲

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黏附素5胞外区克隆、转染及抗人乳腺癌细胞生长的研究

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中国病理生理杂志 2007年第9期23卷 1710-1715页

作者：石小玉李文林熊丽霞张际青熊建军李红南昌大学医学院江西省医学生物高技术重点实验室 DIFEMA Faculté de Médecine et de Pharmacie de Rouen76000 RouenFrance CNRS

目的:克隆和转染黏附素5胞外区,并研究其对人乳腺癌MDA-MB435细胞株生长的影响。方法:RT-PCR技术克隆黏附素5胞外区(称为CED1-4),将其插入pMSCV质粒载体,在大肠杆菌XL-blue扩增,提取和纯化pMSCV-CED1-4,酶切、电泳和测序检测CED1-4序列... 详细信息

目的:克隆和转染黏附素5胞外区,并研究其对人乳腺癌MDA-MB435细胞株生长的影响。方法:RT-PCR技术克隆黏附素5胞外区(称为CED1-4),将其插入pMSCV质粒载体,在大肠杆菌XL-blue扩增,提取和纯化pMSCV-CED1-4,酶切、电泳和测序检测CED1-4序列。CED1-4基因转染MDA-MB435细胞株,RT-PCR和Western blotting检测MDA-MB435细胞表达CED1-4。细胞增殖实验和乳腺癌裸小鼠致瘤实验检测CED1-4对MDA-MB435细胞体内外生长的影响。结果:构建了重组体pMSCV-CED1-4,电泳显示CED1-4条带在1636bp-1018bp区间,测序显示CED1-4基因长1452bp,编码484氨基酸。经PCR和Western blotting证实,CED1-4基因转染的MDA-MB435细胞在mRNA和蛋白质水平表达CED1-4。细胞增殖实验结果表明,实验组MDA-MB435细胞增殖低于实验对照组和空白对照组(P<0.05)。乳腺癌裸小鼠致瘤实验显示,实验组移植瘤平均体积和重量低于实验对照组和空白对照组(P<0.05)。结论:黏附素5胞外区CED1-4能在体内外抑制人乳腺癌MDA-MB435细胞株的生长。

关键词：钙粘着糖蛋白类克隆乳腺肿瘤基因转染

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