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Systemic delivery of microRNA for treatment of brain ischemia

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Nano Research 2021年第9期14卷 3319-3328页

作者： Chaoyong Liu Jing Wen Dai Li Hongzhao Qi Lina Nih Jialin Zhu Duo Xu Yu Ren Shanshan Zhang Donglin Han Huanhuan Jia Junhu Zhou Meng Qin Jialing Wu Xubo Yuan Ji Liu Jin Zhao Chunsheng Kang Yunfeng Lu Department of Neurosurgery Tianjin Medical University General HospitalTianjin 300052China Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System Ministry of EducationTianjin Key Laboratory of InjuriesVariations and Regeneration of Nervous SystemTianjin Neurological InstituteTianjin 300052China College of Life Science and Technology and Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing 100029China Department of Chemical and Biomolecular Engineering University of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Medicine Tianjin Huanhu HospitalTianjin 300350China Tianjin Key Laboratory of Composite and Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin 300350China Department of Microbiology Immunology and Molecular GeneticsUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA Tianjin Research Center of Basic Medical Science Tianjin Medical UniversityTianjin 300070China Department of Neurology Tianjin Huanhu HospitalTianjin 300350China

Brain ischemia is the second leading cause of death and the third leading cause of disability in the *** delivery of microRNA,a class of molecules that regulate the expression of cellular proteins associated with angiogenesis,cell growth,proliferation and differentiation,holds great promise for the treatment of brain ***,their therapeutic efficacy has been hampered by poor delivery efficiency of *** report herein a platform technology based on microRNA nanocapsules,which enables their effective delivery to the disease sites in the *** by microRNA-21,intravenous injection of the nanocapsules into a rat model of cerebral ischemia could effectively ameliorate the infarct volume,neurological deficit and histopathological severity.

关键词： polymer nanocapsules small RNA delivery microRNA-21 brain ischemia

The clinical effectiveness and safety of using epidermal growth factor,fibroblast growth factor and granulocyte-macrophage colony stimulating factor as therapeutics in acute skin wound healing:a systematic review and meta-analysis

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Burns & trauma 2022年第1期10卷 715-741页

作者： Yating Wei Jiangfeng Li Yao Huang Xun Lei Lijun Zhang Meifang Yin Jiawen Deng Xiaoyan Wang Xiaobing Fu Jun Wu Department of Burn and Plastic Surgery Department ofWound RepairShenzhen Institute of Translational Medicinethe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People’s HospitalShenzhenChina Shenzhen University ShenzhenChina School of Public Health and Management Chongqing Medical UniversityChongqingChina The First Affiliated Hospital Sun Yat-sen UniversityGuangzhouChina Research Center for wound Repair and Tissue Regeneration Medical Innovation Research DepartmentThe Key Laboratory of PLAWound Repair and Tissue Regenerationthe Fourth Medical Center of PLA General Hospitalthe PLA General HospitalBeijing 100048China

Background:Promoting wound healing is crucial to restore the vital barrier function of injured *** factor products including epidermal growth factor(EGF),fibroblast growth factor(FGF)and granulocyte-macrophage colony stimulating factor(GM-CSF)have been used for decades although no systematic evaluation exists regarding their effectiveness and safety issues in treating acute skin *** has resulted in a lack of guidelines and standards for proper application ***,this systematic review and meta-analysis was performed to critically evaluate the effectiveness and safety of these growth factors on skin acute wounds and provide guidelines for application ***:We searched PubMed/Medline(1980-2020),Cochrane Library(1980-2020),Cochrane CENTRAL(from establishment to 2020),***(from establishment to 2020),Chinese Journal Full-text Database(CNKI,1994-2020),China Biology Medicine disc(CBM,1978-2019),Chinese Scientific Journal Database(VIP,1989-2020)andWanfang Database(WFDATA,1980-2019).Randomized controlled trials(RCTs),quasi-RCTs and controlled clinical trials treating patients with acute skin wounds from various causes and with those available growth factors were ***:A total of 7573 papers were identified through database searching;229 papers including 281 studies were kept after final *** growth factors significantly short-ened the healing time of acute skin wounds,including superficial burn injuries[mean differ-ence(MD)=−3.02;95%confidence interval(CI):−3.31∼−2.74;ptraumata and surgical wounds(MD=−4.50;95%CI:−5.55∼−3.44;pwounds and decreased scar *** incidence of adverse reactions was lower in the growth factor treatment group than in the non-growth factor ***:The studied growth factors not only are effective and safe for managing acute sk

关键词： Growth factors Skin wounds Meta-analysis wound healing

The efficacy assessment of convalescent plasma therapy for COVID-19 patients:a multi-center case series

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Signal Transduction and Targeted Therapy 2020年第1期5卷 651-662页

作者： Hao Zeng Dongfang Wang Jingmin Nie Haoyu Liang Jiang Gu Anne Zhao Lixin Xu Chunhui Lang Xiaoping Cui Xiaolan Guo Changlong Zhou Haibo Li Bin Guo Jinyong Zhang Qiang Wang Li Fang Wen Liu Yishan Huang Wei Mao Yaokai Chen Quanming Zou National Engineering Research Center of Immunological Department of Microbiology and Biochemical PharmacyCollege of Pharmacy and Laboratory MedicineThird Military Medical UniversityChongqingP.R.China State Key Laboratory of trauma Burn and Combined InjuryThird Military Medical UniversityChongqingP.R.China Institute of Blood Transfusion Chongqing Blood CenterChongqingP.R.China Chongqing Public Health Medical Central ChongqingP.R.China Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug ControlBeijingP.R.China Chongqing University Three Gorges Hospital&Chongqing Three Gorges Central Hospital ChongqingP.R.China Affiliated Hospital of North Sichuan Medical College NanchongP.R.China Yongchuan Hospital of Chongqing Medical University ChongqingP.R.China

Convalescent plasma(CP)transfusion has been indicated as a promising therapy in the treatment for other emerging viral ***,the quality control of CP and individual variation in patients in different studies make it rather difficult to evaluate the efficacy and risk of CP therapy for coronavirus disease 2019(COVID-19).We aimed to explore the potential efficacy of CP therapy,and to assess the possible factors associated with its *** enrolled eight critical or severe COVID-19 patients from four *** patient was transfused with 200–400mL of CP from seven recovered *** primary indicators for clinical efficacy assessment were the changes of clinical symptoms,laboratory parameters,and radiological image after CP *** donors had a wide range of antibody levels measured by serology tests which were to some degree correlated with the neutralizing antibody(NAb)*** adverse events were observed during and after CP *** CP transfusion,six out of eight patients showed improved oxygen support status;chest CT indicated varying degrees of absorption of pulmonary lesions in six patients within 8 days;the viral load was decreased to a negative level in five patients who had the previous viremia;other laboratory parameters also tended to improve,including increased lymphocyte counts,decreased C-reactive protein,procalcitonin,and indicators for liver *** clinical efficacy might be associated with CP transfusion time,transfused dose,and the NAb levels of *** study indicated that CP might be a potential therapy for severe patients with COVID-19.

关键词： patients transfusion fused

Emerging role of tumor cell plasticity in modifying therapeutic response

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Signal Transduction and Targeted Therapy 2020年第1期5卷 549-584页

作者： Siyuan Qin Jingwen Jiang Yi Lu Edouard CNice Canhua Huang Jian Zhang Weifeng He State Key Laboratory of Biotherapy and Cancer Center West China Hospitaland West China School of Basic Medical Sciences and Forensic MedicineSichuan Universityand Collaborative Innovation Center for Biotherapy610041 ChengduPeople’s Republic of China School of Medicine Southern University of Science and Technology ShenzhenShenzhenGuangdong 518055People’s Republic of China Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research ShenzhenGuangdongPeople’s Republic of China Department of Biochemistry and Molecular Biology Monash UniversityClaytonVICAustralia School of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine1166 Liutai Road611137 ChengduPeople’s Republic of China State Key Laboratory of trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingPeople’s Republic of China Chongqing Key Laboratory for Disease Proteomics ChongqingPeople’s Republic of China

Resistance to cancer therapy is a major barrier to cancer *** views have proposed that acquisition of resistance may result from genetic ***,accumulating evidence implicates a key role of non-mutational resistance mechanisms underlying drug tolerance,the latter of which is the focus that will be discussed *** non-mutational processes are largely driven by tumor cell plasticity,which renders tumor cells insusceptible to the drug-targeted pathway,thereby facilitating the tumor cell survival and *** concept of tumor cell plasticity highlights the significance of re-activation of developmental programs that are closely correlated with epithelial–mesenchymal transition,acquisition properties of cancer stem cells,and transdifferentiation potential during drug *** observations in various cancers,this concept provides an opportunity for investigating the nature of anticancer drug *** the years,our understanding of the emerging role of phenotype switching in modifying therapeutic response has considerably *** expanded knowledge of tumor cell plasticity contributes to developing novel therapeutic strategies or combination therapy regimens using available anticancer drugs,which are likely to improve patient outcomes in clinical practice.

关键词： therapeutic plasticity thereby

Prediction of T cell and B cell epitopes of the 22-, 47-, 56-, and 58-kDa proteins of Orientia tsutsugamushi

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Asian Pacific Journal of Tropical Biomedicine 2019年第10期9卷 443-448页

作者： Li-Na Niu Ting-Ting Fu Man-Ling Chen Yu-Ying Dong Jin-Chun Tu Zi-Hao Wang Si-Qi Wang Xuan Zhao Nai-Xu Hou Qian Chen Qiang Wu Laboratory of Tropical Biomedicine and Biotechnology School of Tropical Medicine and Laboratory MedicineHainan Medical UniversityHaikou Hainan 571199China Department of Laboratory the First Affiliated Hospital of Hainan Medical UniversityHaikou Hainan 570102China Department of Gynecology the First Affiliated Hospital of Hainan Medical UniversityHaikou Hainan 570102China State Key Laboratory of Marine Resource Utilization in South China Sea College of Material science and EngineeringHainan UniversityHaikou Hainan 570228 China 5Department of Pathogen BiologySchool of Basic Medicine and Life ScienceHainan Medical UniversityHainan Medical University-University of Hong Kong Joint Laboratory of Tropical Infectious DiseasesHaikou Hainan 571199China Key Laboratory of Emergency and trauma of Ministry of Education&Research Unit of Island Emergency Medicine of Chinese Academy of Medical Sciences Hainan Medical UniversityHaikou Hainan 571199China

Objective:To predict B cell and T cell epitopes of 22-kDa,47-kDa,56-kDa and 58-kDa ***:The sequences of 22-kDa,47-kDa,56-kDa and 58-kDa proteins which were derived from Orientia tsutsugamushi were analyzed by SOPMA,DNAstar,Bcepred,ABCpred,NetMHC,NetMHCⅡand *** 58-kDa tertiary structure model was built by ***:The 22-kDa B-cell epitopes were located at positions 194-200,20-26 and 143-154,whereas the T-cell epitopes were located at positions 154-174,95-107,17-25 and *** 47-kD a protein B-cell epitopes were at positions 413-434,150-161 and 283-322,whereas the T-cell epitopes were located at positions 129-147,259-267,412-420 and *** 56-kDa protein B-cell epitopes were at positions 167-173,410-419 and 101-108,whereas the T-cell epitopes were located at positions 88-104,429-439,232-240 and *** 58-kDa protein B-cell epitopes were at positions 312-317,540-548 and 35-55,whereas the T-cell epitopes were located at positions 415-434,66-84 and ***:We identified candidate epitopes of 22-kDa,47-kDa,56-kDa and 58-kDa proteins from Orientia *** the case of 58-kDa,the dominant antigen is displayed on tertiary structure by homology *** findings will help target additional recombinant antigens with strong specificity,high sensitivity,and stable expression and will aid in their isolation and purification.

关键词： Orientia tsutsugamushi B cell epitopes T cell epitopes Bioinformatic prediction

Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第12期24卷 1165-1173页

作者： Yanhua MU Jinhua HAN Mingjie WU Zongfang LI Ke DU Yameng WEI Mengjie WU Jun HUANG National-Local Joint Engineering Research Center of Biodiagnosis&Biotherapy The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004China The MOE Key Laboratory of Biosystems Homeostasis&Protection and Innovation Centerfor Cell Signaling Network Life Sciences InstituteZhejiang UniversityHangzhou 310058China Zhejiang Provincial Key Lab of Geriatrics and Geriatrics Institute of Zhejiang Province Department of GeriatricsZhejiang Hospital.Hangzhou310030China trauma Center The First Afiliated HospitalZhejiang University School of MedicineHangzhou 310003China The Afiliated Hospitalof Stomatology Schoolof StomatologyZhejiang University Schoolof MedicineKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhou 310006China Zhejiang Provincial Key Labratoryof Cancer Molecular Cell Biology Life Sciences InstituteZhejiang UniversityHangzhou 30058China Department of General Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China

Eukaryotic organisms constantly face a wide range of internal and external factors that cause damage to their *** to accurately and efficiently repair these DNA lesions can result in genomic instability and the development of tumors(Canela et al.,2017).Among the various forms of DNA damage,DNA doublestrand breaks(DSBs)are particularly *** major pathways,non-homologous end joining(NHEJ)and homologous recombination(HR),are primarily responsible for repairing DSBs(Katsuki et al.,2020;Li and Yuan,2021;Zhang and Gong,2021;Xiang et al.,2023).NHEJ is an error-prone repair mechanism that simply joins the broken ends together(Blunt et al.,1995;Hartley et al.,1995).

关键词： damage homologous

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贫血与脑损伤：问题多，答案少

麻醉与镇痛 2009年第6期5卷 1-15页

作者： Gregory M. T. Hare Albert K. Y. Tsui Anya T. McLaren Tenille E. Ragoonanan Julie Yu C. David Mazer, MD 宋云(译) 闵苏(校) Department of Anesthesia Cara Phelan Trauma Research Centre Keenan Research Centre in the Li Ka Shing Knowledge Institute University of Toronto St. Michael's Hospital Toronto Ontario Department of Physiology University of Toronto Toronto Ontario 不详

大量临床研究显示围手术期患者的脑损伤与急性贫血有关。这种损伤发生在接近于我们普遍认同的输血指征[血红蛋白（Hb）浓度7～8g/dl]，但高于脑组织缺氧的血红蛋白浓度（Hb3～4g／dl）的情况下。但是，贫血引起脑损伤的缺氧和非缺氧机... 详细信息

大量临床研究显示围手术期患者的脑损伤与急性贫血有关。这种损伤发生在接近于我们普遍认同的输血指征[血红蛋白（Hb）浓度7～8g/dl]，但高于脑组织缺氧的血红蛋白浓度（Hb3～4g／dl）的情况下。但是，贫血引起脑损伤的缺氧和非缺氧机制仍不清楚。此外，能最大程度降低急性贫血所致脑损伤的保护性机制仍未阐明。包括一氧化氮（NO）在内的血管扩张机制，在贫血时有助于维持大脑氧供，在各种急性稀释性缺血试验模型中，3种NO合成酶（NOS）（神经型、内皮型及可诱导型NOS）都有上调。近来的实验也证实了在相当于临床缺氧Hb浓度（Hb6—7g/dl）的啮齿类动物的大脑皮质中，重要的转录因子如缺氧诱导因子（HIF）-1α有所增加。这说明急性贫血对脑氧的供需平衡可能造成威胁，在缺氧的情况下，细胞浆中的HIF-1α降解被抑制，HIF-1α积聚成二聚体，移位到细胞核中并增加许多低氧分子的转录。这些低氧分子在贫血的脑组织中的表达也被发现上调，包括红细胞生成素，血管内皮生长因子，可诱导型NOS。此外，非低氧介质，包括细胞因子和血管激素，可以增加HIF-1α的转录。而且，源于NOS的NO也可以在没有组织缺氧时稳定HIF—1α。因此，在贫血时，HIF-1α在低氧和常氧情况下能对脑细胞进行调节。实验研究发现，针对不同类型的细胞，上调的HIF-1α可以表现为神经保护作用或神经毒性作用。在本综述中，我们描述了这些细胞作用过程，对贫血引起的脑损伤和保护机制有了更清晰的认识。贫血引起损伤的可能机制包括脑栓塞、组织缺氧、炎症、活性氧和兴奋性毒性。可能的细胞保护机制包括NOS／NO依赖的细胞氧供增强和包括HIF-1α、红细胞生成素及血管内皮生长因子在内的细胞保护机制。这些激活的细胞机制的总体平衡决定着它们在贫血时的上调是起细胞保护作用还是细胞损伤作用。深入了解这些机制有助于明确什么样的治疗措施可以降低围手术期贫血性脑损伤。

关键词：脑损伤缺氧诱导因子(HIF)-1α 贫血血管内皮生长因子 HIF-1α 诱导型NOS 脑组织缺氧血红蛋白浓度

Inwardly rectifying potassium channel 5.1:Structure,function,and possible roles in diseases

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Genes & Diseases 2021年第3期8卷 272-278页

作者： Junhui Zhang Jian Han Lingfei Li Qiong Zhang Yanhai Feng Youzhao Jiang Fang Deng Yuping Zhang Qinan Wu Bing Chen Jiongyu Hu Institute of Burn Research Southwest HospitalThird Military Medical University(Army Medical University)Chongqing400038PR China State Key Laboratory of trauma Burns and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing400038PR China Department of Obstetrics and Gynecology Daping HospitalThird Military Medical University(Army Medical University)Chongqing400042PR China Department of Dermatology Daping HospitalThird Military Medical University(Army Medical University)Chongqing400042PR China Department of Endocrinology People’s Hospital of Banan DistrictChongqing401320PR China Department of Endocrinology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing400038PR China Department of Endocrinology Chongqing Cancer Hospital(Chongqing University Cancer Hospital)Chongqing40030PR China

Inwardly rectifying potassium(Kir)channels make it easier for K^(+)to enter into a cell and subsequently regulate cellular biological ***5.1(encoded by KCNJ16)alone can form a homotetramer and can form heterotetramers with Kir4.1(encoded by KCNJ10)or Kir4.2(encoded by KCNJ15).In most cases,homomeric Kir5.1 is non-functional,while hetero-meric Kir5.1 on the cell membrane contributes to the inward flow of K^(+)ions,which can be regulated by intracellular pH and a variety of signaling *** the form of a heterotetramer,Kir5.1 regulates Kir4.1/4.2 activity and is involved in the maintenance of nephron ***,homomeric Kir5.1 may also play a very important role in diseases,including in the ventilatory response to hypoxia and hypercapnia,hearing impairment,cardio-vascular disease and *** an increase in the number of studies into the roles of Kir channels,researchers are paying more attention to the pathophysiological functions of *** minireview provides an overview regarding these Kir5.1 roles.

关键词： Cancer Cardiovascular disease Cochlea Kir5.1 heterotetramer Ventilatory response

Epidemiological Characteristics and Disease Burden of Burns in Children in Northern Guizhou, China

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Chinese Medical Journal 2018年第17期131卷 2125-2127页

作者： Tao Wang Chan Nie Hong Zhang Xue-Qin Zeng Hui-Ting Yu Shang-Peng Shi Zai-Rong Wei Xiu-Quan Shi Department of Epidemiology and Health Statistics School of Public Health Zunyi Medical University Zunyi Guizhou 563006 China Center forMedical Records Manaqement Affiliated Hospital of Zunyi Medical University Zunyi Guizhou 563000 China Department of Burns and Plastic Surqerv Affiliated Hospital of Zunyi Medical University Zunyi Guizhou 563000 China Center for Injury Research and Policy Center for Pediatric Trauma Research The Research Institute at Nationwide Children's Hospital The Ohio State University College of Medicine Columbus OH USA

To the Editor： Bum is a type of injury that is common to children and is likely to endanger the child＇s lifelong health, reduce the family＇s well-being, and increase the social burden of disease and economic losses. A 2017 report by the World Health Organization indicated that an estimated 180,000 deaths every year were caused by bums, which occurred especially in low- and middle-income cotlntries.

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