检索结果-南通市图书馆

4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2023年第3期14卷 202-216页

作者： Yifang He Qianzhao Ji Zeming Wu Yusheng Cai Jian Yin Yiyuan Zhang Sheng Zhang Xiaoqian Liu Weiqi zhang Guang-Hui Liu Si Wang Moshi Song Jing Qu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 10o101China Beijing institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China Advanced Innovation Center for Human brain Protection and National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing100053China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Beijing 100101China National Laboratory of Biomacromolecules CAs Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101 China State Key Laboratory of brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of Brain-Intelligence Technology(Shanghai)Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Aging translational Medicine Center Intermational Center for Aging and CancerBejing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China The Fifth People's Hospital of Chongqing Chongqing 400062China institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China

Although the mTOR-4E-BP1 signaling pathway is implicated in aging and aging-related disorders,the role of 4E-BP1 in regulating human stem cell homeostasis remains largely unknown.Here,we report that the expression of 4E-BP1 decreases along with the senescence of human mesenchymal stem celis(hMSCs).Genetic inactivation of 4E-BP1 in hMSCs compromises mitochondrial respiration,increases mitochondrial reactive oxygen species(Ros)production,and accelerates cellular senescence.Mechanistically,the absence of 4E-BP1 destabilizes proteins in mitochondrial respiration complexes,especially several key subunits of complex III including UQCRC2.Ectopic expression of 4E-BP1 attenuates mitochondrial abnormalities and alleviates cellular senescence in 4E-BP1-deficient hMSCs as well as in physiologically aged hMSCs.These findings together demonstrate that 4E-BP1 functions as a geroprotector to mitigate human stem cell senescence and maintain mitochondrial homeostasis,particularly for the mitochondrial respiration complex Il,thus providing a new potential target to counteract human stem cell senescence.

关键词： 4E-BP1 mitochondria aging

DNA bridging of FOXP3 ladder-like multimer: Unveiling a novel transcriptional regulation paradigm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Innovation 2024年第3期5卷 15-16页

作者： Ying Lu Lanfang Li Hanting Yang Bin Li Zuoyun Wang Key Laboratory of Metabolism and Molecular Medicine of the Ministry of Education Shanghai Xuhui Central Hospital and School of Basic Medical SciencesFudan UniversityShanghai 200032China institute for translational brain research State Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain ScienceDepartment of NeurologyZhongshan HospitalFudan UniversityShanghai 200032China Center for Immune-Related Diseases at Shanghai institute of Immunology Department of Immunology and MicrobiologyShanghai Jiao Tong University School of MedicineShanghai 200025China

In the latest issue of Nature,Zhang et al.characterized a novel ladder-like structure of FOXP3-DNA interaction involving FOXP3 multimerization and remote DNA bridging through a combination of biochemistry,structural biology,cell biology,and bioinformatics analyses.1 In this commentary,we highlight their key findings and provide our insights into the research paradigm for further exploration of a novel transcriptional regulation mode as well as a therapeutic strategy from the structural aspects of the FOXP3 complex(Figure 1).

关键词： FOXP3 bridging

Astrocytic Gap Junctions Contribute to Aberrant Neuronal Synchronization in a Mouse Model of MeCP2 Duplication Syndrome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2022年第6期38卷 591-606页

作者： Shengnan Xia Hua-Tai Xu institute of Neuroscience and State Key Laboratory of Neuroscience CAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Seciences Beijing 100049.China Shanghai Center for brain Science and brain-Inspired intelligence Technology Shanghai 201210China

Abnormal synchronous neuronal activity has been widely detected by brain imaging of autistic patients,but its underlying neural mechanism remains unclear.Compared with wild-type mice,our in vivo two-photon imaging showed that transgenic(Tgl)mice over-expressing human autism risk gene MeCP2 exhibited higher neuronal synchrony in the young but lower synchrony in the adult stage.Whole-cell recording of neuronal pairs in brain slices revealed that higher neuronal synchrony in young postnatal Tgl mice was atributed mainly to more prevalent giant slow inward currents(SICs).Both in vivo and slice imaging further demonstrated more dynamic activity and higher synchrony in astrocytes from young Tgl mice.Blocking astrocytic gap junctions markedly decreased the generation of SICs and overall cell synchrony in the Tgl brain.Furthermore,the expression level of Cx43 protein and the coupling efficiency of astrocyte gap junctions remained unchanged in Tgi mice.Thus,astrocytic gap junctions facilitate but do not act as a direct trigger for the abnormal neuronal synchrony in young Tgl mice,revealing the potential role of the astrocyte network in the pathogenesis of MeCP2 duplication syndrome.

关键词： Autism spectrum disorder Astrocyte Gap junction Neuronal synchrony MeCP2-Slow inward current Neuronal development

Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2019年第1期6卷 101-108页

作者： Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun institute of Neuroscience CAS Center for Excellence in Brain Science and Intelligence Technology CAS Key Laboratory of Primate Neurobiology State Key Laboratory of Neuroscience Chinese Academy of Sciences Shanghai research Center for brain Science and brain-inspired Technology

Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds.

关键词： macaque monkey cloning somatic cell nuclear transfer non-human primate models CRISPR/Cas9 gene editing circadian rhythm disorders

From Parametric Representation to Dynamical System: Shifting Views of the Motor Cortex in Motor Control

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2022年第7期38卷 796-808页

作者： Tianwei Wang Yun Chen He Cui Center for Excellence in brain Science and Intlligent Technology Institute of NeuroscienceChinese Academy of SciencesShanghai 200031China Shanghai Center for brain and brain-inspired intelligence Technology Shanghai 200031China University of Chinese Academy of Sciences Beijing 100049China

In contrast to traditional representational perspectives in which the motor cortex is involved in motor control via neuronal preferenceforkinetics andkinematics,a dynamical system perspective emerging in the last decade views the motor cortex as a dynamical machine that generates motor commands by autonomous temporal evolution.In this review,we first look back at the history of the representational and dynamical perspectives and discuss their explanatory power and controversy from both empirical and computational points of view.Here,we aim to reconcile the above perspectives,and evaluate their theoretical impact,future direction,and potential applications in brain-machine interfaces.

关键词： Dimensionality reduction Neural network Machine learning Population decoding brain-machine interface

Novel Microglia-based Therapeutic Approaches to Neurodegenerative Disorders

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2023年第3期39卷 491-502页

作者： Lijuan Zhang Yafei Wang Taohui Liu Ying Mao Bo Peng Department of Neurosurgery Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain ScienceFudan UniversityShanghai 200040China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226001China

prominent immune cells in the central nervous system,microglia constantly monitor the environment and provide neuronal protection,which are important functions for maintaining brain homeostasis.In the diseased brain,microglia are crucial mediators of neuroinflammation that regulates a broad spectrum of cellular responses.In this review,we summarize current knowledge on the multifunctional contributions of microglia to homeostasis and their involvement in neurodegeneration.We further provide a comprehensive overview of therapeutic interventions targeting microglia in neurodegenerative diseases.Notably,we propose microglial depletion and subsequent repopulation as promising replacement therapy.Although microglial replacement therapy is still in its infancy,it will likely be a trend in the development of treatments for neurodegenerative diseases due to its versatility and selectivity.

关键词： Microglia Neuroinflammation Multifunction Microglial replacement Neurodegeneration

Rapid Unconscious Acquisition of Conditioned Fear with Low-Spatial-Frequency but Emotionally Neutral Stimuli

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

research 2024年第1期2023卷 753-762页

作者： Yujie Chen Si Chen Zhongju Sun Xilei Zhang Xiangyong Yuan Liang Wang Yi Jiang State Key Laboratory of brain and Cognitive Sciences CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of PsychologyChinese Academy of SciencesBeijing 100101China Department of Psychology University of Chinese Academy of SciencesBeijing 100049China Chinese institute for brain research Beijing 102206China CAS Key Laboratory of Mental Health Institute of PsychologyChinese Academy of SciencesBeijing 100101China

It has long been proposed that emotionally“prepared”(i.e.,fear-related)stimuli are privileged in the unconscious acquisition of conditioned fear.However,as fear processing is suggested to highly depend on the coarse,low-spatial-frequency(LSF)components of the fear-related stimuli,it is plausible that LSF may play a unique role in the unconscious fear conditioning even with emotionally neutral stimuli.Here,we provided empirical evidence that,following classical fear conditioning,an invisible,emotionally neutral conditioned stimulus(CS+)with LSF,but not with high spatial frequency(HSF),can rapidly elicit stronger skin conductance responses(SCRs)and larger pupil diameters than its CS−counterpart.In comparison,consciously perceived emotionally neutral CS+with LSF and HSF elicited comparable SCRs.Taken together,these results support that the unconscious fear conditioning does not necessarily entail emotionally prepared stimuli but prioritizes LSF information processing and highlight the crucial distinctions between the unconscious and the conscious fear learning.These findings not only coincide with the postulation that a rapid,spatial-frequency-dependent subcortical route is engaged in unconscious fear processing but also suggest the existence of multiple routes for conscious fear processing.

关键词： neutral necessarily diameters

Lypd1-DTR/+: A New Mouse Model for Specifically Damaging the Type Ic Spiral Ganglion Neurons of the Cochlea

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2023年第9期39卷 1459-1462页

作者： Di Zhang Minhui Ren ZhengHong Bi Yunpeng Gu Shuting Li Guangqin Wang Xiang Li Zhiyong Liu institute of Neuroscience State Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai200031China University of Chinese Academy of Sciences Beijing100049China Shanghai Center for brain Science and brain-Inspired intelligence Technology Shanghai201210China

Dear Editor,Inner ear auditory neurons,which are also referred as to spiral ganglion neurons(SGNs),bridge sound-receptor hair cells(HCs)to the cochlear nucleus in the brainstem[1].Two types of SGN exist:type I SGNs(95... 详细信息

关键词： auditory brainstem cochlear

Intestinal Dopamine Receptor D2 is Required for Neuroprotection Against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Dopaminergic Neurodegeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2022年第8期38卷 871-886页

作者： Hairong Peng Shui Yu Yukai Zhang Yanqing Yin Jiawei Zhou institute of Neuroscience State Key Laboratory of NeuroscienceCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai200031China School of Future Technology University of Chinese Academy of SciencesBeijing100049China Shanghai Center for brain Science and brain-Inspired intelligence Technology Shanghai201210China

A wealth of evidence has suggested that gastrointestinal dysfunction is associated with the onset and progression of Parkinson’s disease(PD).However,the mechanisms underlying these links remain to be defined.Here,we investigated the impact of deregulation of intestinal dopamine D2 receptor(DRD2)signaling in response to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced dopaminergic neurodegeneration.Dopamine/dopamine signaling in the mouse colon decreased with ageing.Selective ablation of Drd2,but not Drd4,in the intestinal epithelium,caused a more severe loss of dopaminergic neurons in the substantia nigra following MPTP challenge,and this was accompanied by a reduced abundance of succinate-producing Alleoprevotella in the gut microbiota.Administration of succinate markedly attenuated dopaminergic neuronal loss in MPTP-treated mice by elevating the mitochondrial membrane potential.This study suggests that intestinal epithelial DRD2 activity and succinate from the gut microbiome contribute to the maintenance of nigral DA neuron survival.These findings provide a potential strategy targeting neuroinflammation-related neurological disorders such as PD.

关键词： Intestine DRD2 Parkinson’s disease MPTP Succinate Mitochondria