检索结果-南通市图书馆

A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance

Molecular Plant 2024年第6期17卷 955-971页

作者： Xianhong Wang Ioly Kotta-Loizou Robert H.A.Coutts Huifang Deng Zhenhao Han Ni Hong Karim Shafik Liping Wang Yashuang Guo Mengmeng Yang Wenxing Xu Guoping Wang National Key laboratory for Germplasm Innovation&Utilization of Horticultural Crops Wuhan 430070China Hubei Hongshan laboratory Wuhan 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Key lab of Plant Pathology of Hubei Province Wuhan 430070China Department of Life Sciences Faculty of Natural SciencesImperial College LondonLondon SW72AZUK Department of Clinical Pharmaceutical and Biological ScienceSchool of Life and Medical SciencesUniversity of HertfordshireHatfield AL109ABUK Department of Plant Pathology Faculty of AgricultureAlexandria UniversityAlexandria 21526Egypt

Circular single-stranded DNA(ssDNA)viruses have been rarely found in fungi,and the evolutionary and ecological relationships among ssDNA viruses infecting fungi and other organisms remain *** this study,a novel circular ssDNA virus,tentatively named Diaporthe sojae circular DNA virus 1(DsCDV1),was identified in the phytopathogenic fungus Diaporthe sojae isolated from pear ***1 has a monopartite genome(3185 nt in size)encapsidated in isometric virions(21-26 nm in diameter).The genome comprises seven putative open reading frames encoding a discrete replicase(Rep)split by an intergenic region,a putative capsid protein(CP),several proteins of unknown function(P1-P4),and a long intergenic ***,the two split parts of DsCDV1 Rep share high identities with the Reps of Geminiviridae and Genomoviridae,respectively,indicating an evolutionary linkage with both *** analysis based on Rep or CP sequences placed DsCDV1 in a unique cluster,supporting the establishment of a new family,tentatively named Gegemycoviridae,intermediate to both ***1 significantly attenuates fungal growth and nearly erases fungal virulence when transfected into the host ***,DsCDV1 can systematically infect tobacco and pear seedlings,providing broad-spectrum resistance to fungal *** localization analysis revealed that DsCDV1 P3 is systematically localized in the plasmodesmata,while its expression in trans-complementation experiments could restore systematic infection of a movement-deficient plant virus,suggesting that P3 is a movement ***1 exhibits unique molecular and biological traits not observed in other ssDNA viruses,serving as a link between fungal and plant ssDNA viruses and presenting an evolutionary connection between ssDNA viruses and *** findings contribute to expanding our understanding of ssDNA virus diversity and evolution,offering potential biocontrol applications for managing crucial plant d

关键词： circular single-stranded DNA virus mycovirus virus evolution Genomoviridae Gegemycoviridae Diaporthe sojae circular DNA virus 1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification of proteasome and caspase inhibitors targeting SARS-CoV-2 Mpro

引用

Signal transduction and Targeted Therapy 2021年第7期6卷 1959-1962页

作者： Zhengyuan Wang Yao Zhao Qingxing Wang Yangfei Xing Lu Feng Juan Kong Chao Peng Leike Zhang Haitao Yang Min Lu Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine(Shanghai)Ruijin Hospital affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech UniversityShanghaiChina State Key laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhanChina National Facility for Protein Science in Shanghai Zhangjiang LabShanghai Advanced Research InstituteChinese Academy of ScienceShanghaiChina

Dear Editor,Since the beginning of 2020,the Coronavirus(CoV)Disease 2019(COVID-19)pandemic has posed formidable challenges to public health *** main protease(Mpro,3CLpro)of CoVs plays essential roles in viral replicat... 详细信息

关键词： attractive development caspase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation of Intelligent Supramolecular Hydrogels and Its application as 3D culture scaffold of ovarian cancer cell

引用

功能材料信息 2016年第4期13卷 55-56页

作者： Tianzhu Zhang Xiaoe Ma Naizheng Zhou Junsong Chen Jun Dou Ning Gu State Key laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science and Medical EngineeringSoutheast University Suzhou Key lab oratory of Biomedical Materials and Technology Research Institute of Southeast University in Suzhou Department of Pathogenic Biology and Immunology Medical SchoolSoutheast University

Destination:to prepare intelligent suparmolecular hydrogel by mimicking the key features of extracellular matrices(ECM)and culture ovarian cancer cells in 3D mold,then investigate the influence of microenvironments on... 详细信息

关键词：英语阅读理解生物材料

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparation, structure and properties of fluorescent nano- CdTe/poly （1, 4-butanediol-citrate） bioelastomer nanocomposite in-situ dispersion technique

引用

Frontiers of Optoelectronics 2013年第4期6卷 452-457页

作者： Li JIANG Aimiao QIN Kunpeng JIANG Lei LIAO Xiulan WU Chaojian WU Key lab of New Processing Technology for Nonferrous Metals & Materials Ministry of Education College of Materials Science & Engineering Guilin University of Technology Guilin 541004 China Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou 350002 China College of Environmental Science & Engineering Guilin University of Technology Guilin 541004 China

Hydrophilic photoluminescent CdTe/poly （1, 4-butanediol-citrate）（PBC） bioelastomer nanocomposite was successfully synthesized by a two-step method and characterized by X-ray diffraction （XRD）, Fourier trans- form infrared （FT-IR） spectroscopy, Uv-vis spectroscopy, photoluminescence （PL） spectroscopy and scanning elec- tron microscope （SEM）. The differential scanning calori- metry analysis shows that the bioelastomer nanocom- posites with different mass fractions of CdTe have low glass-transition temperature, which indicates that they possess elastic property in the range from room tempera- ture to the expected applied temperature （37℃）. The measurements of the hydrophilicity, in vitro degradation and PL show that the nanocomposite has good hydro- philicity, degradation and high fluorescence properties.

关键词： bioelastomer spectroscopy biodegradable

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

No association between phosphatase and tensin homolog genetic polymorphisms and colon cancer

引用

World Journal of Gastroenterology 2009年第30期15卷 3771-3775页

作者： Lynette S Phillips Cheryl L Thompson Alona Merkulova Sarah J Plummer Thomas C Tucker Graham Case Li Li Department of Epidemiology and Biostatistics and Case Comprehensive Cancer Center Case Western Reserve University Cleveland OH 44106-7136 United States Departments of Family Medicine and Epidemiology and Biostatistics Case Center for Transdisciplinary Research on Energetics and Cancer Case Comprehensive Cancer Center Case Western Reserve University Cleveland OH 44106-7136m United States Department of Cancer Biology Cleveland Clinic Foundation Cleveland OH 44195-0001 United States Graham Casey Department of Preventive Medicine University of Southern California Los Angeles CA 90033-1006 United States assisted with patient referrals recruitment and reviewed the manuscript coordinated the lab work and reviewed the manuscript Li L led the study design data collection and assisted with the manuscript preparation

AIM: To investigate the association between single nucleotide polymorphisms (SNPs) in the phosphatase and tensin homolog (PTEN) tumor suppressor gene and risk of colon cancer. METHODS: We utilized a population-based casecontrol study of incident colon cancer individuals (n= 421) and controls (n = 483) aged ≥ 30 years to conduct a comprehensive tagSNP association analysis of the PTEN gene. RESULTS: None of the PTEN SNPs were statistically significantly associated with colon cancer when controlled for age, gender, and race, or when additionally adjusted for other known risk factors (P > 0.05). Haplotype analyses similarly showed no association between the PTEN gene and colon cancer. CONCLUSION: Our study does not support PTEN as a colon cancer susceptibility gene.

关键词： Colon cancer Phosphatase and tensinhomolog Candidate gene Genetic polymorphisms Single nucleotide polymorphism association

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

引用

Bioactive Materials 2021年第12期6卷 4580-4590页

作者： Jiaojiao Gong Lijuan Kan Xiuming Zhang Ying He Jiaqiang Pan Liping Zhao Qianyun Li Menghao Liu Jie Tian Sili Lin Zhouyu Lu Liang Xue Chaojun Wang Guanghui Tang Yaneng Biotech Co.LtdFosun PharmaShenzhen 518100China Department of Urology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Department of laboratory Medicine Luohu District People’s HospitalShenzhen 518001China Department of laboratory Medicine The Eighth Affiliated HospitalSun Yat-sen UniversityShenzhen 518033China Department of laboratory Medicine Nanning First People’s HospitalNanning 530022China Department of Neurology Hwa Mei HospitalUniversity of Chinese Academy of SciencesNingbo 315010China Nanobiological Medicine Center Key Lab of Fuel Cell Technology of Guangdong ProvinceSchool of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510641China

CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid,portable and accurate ***,cleavage of the amplicons and primers by the cis-and trans-activity of Cas12a hinders the attempts to integrate the amplification and detection into a single *** phosphorothioate modification of primers,we realized onepot detection with high sensitivity using plasmids of SARS-CoV-2,HPV16 and *** also identified the activated Cas12a has a much higher affinity to C nucleotide-rich reporter than *** applying such reporters,the reaction time required for a lateral-flow readout was significantly ***,to improve the specificity of the strip-based assay,we created a novel reporter and,when combined with a customized gold-nanopaticle strip,the readout was greatly enhanced owing to the elimination of the nonspecific *** established system,termed Targeting DNA by Cas12a-based Eye Sight Testing in an Onepot Reaction(TESTOR),was validated using clinical cervical scrape samples for human papillomaviruses(HPVs)*** system represents a general approach to integrating the nucleic acid amplification and detection into a single reaction in CRISPR-Cas systems,highlighting its potential as a rapid,portable and accurate detection platform of nucleic acids.

关键词： CRISPR-Cas12a Onepot Visual detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

trans-Gulf of Mexico loop migration of tree swallows revealed by solar geolocation

引用

Current Zoology 2014年第5期60卷 653-659页

作者： David W. BRADLEY Robert G. CLARK Peter O. DUNN Andrew J. LAUGHLIN Caz M. TAYLOR Carol VLECK Linda A. WHITTINGHAM David W. WINKLER D. Ryan NORRIS Department of Integrative Biology University of Guelph Guelph Ontario N1G 2W 1 Canada Bird Studies Canada Port Rowan Ontario N0E 1M0 Canada Environment Canada. Saskatoon. Saskatchewan S7N 0X4. Canada Behavioral and Molecular Ecology Group Department of Biological Sciences University of Wisconsin Milwaukee Wisconsin 53201 USA Department of Ecology and Evolutionary Biology Tulane University New Orleans Louisiana 70118 USA Department of Ecology Evolution and Organismal Biology Iowa State University Ames IA 50011-1020 USA Department of Ecology and Evolutionary Biology Museum of Vertebrates and Lab of Ornithology Comell University Ithaca New York 14853 USA

One of the greatest feats of avian migration is the non-stop crossing of extensive areas of inhospitable habitat such as deserts and seas. Differences in spring and autumn migration routes have been reported in species that cross such barriers, and are thought to have evolved in response to seasonal variation in prevailing wind direction. We tested the hypothesis that migration routes vary seasonally with respect to the Gulf of Mexico in the tree swallow Tachycineta bicolor using solar geolocators attached and retrieved at 4 breeding sites in central North America. We found that 100 % of birds （n = 10） made a trans-Gulf flight of 〉850 km from Louisiana south to their wintering grounds in the Yucatan Peninsula in 12-36 hours, achieving minimum ground speeds as high as 32 m/s. Although most days during autumn migration were characterized by unfavorable headwinds blowing to the northwest, migration over the Gulf mostly occurred on days with strong winds blowing to the south. In contrast, in 8 of 9 （88 %） birds on spring migration returned from the wintering grounds towards Louisiana following a clockwise loop pat- tern flying over land to the west around the Gulf. During this spring period there were few days with prevailing winds from the south to assist northward migration. Results suggest that, despite being up to three times further （ca. 2,700 kin）, a coastal circum-Gulf spring migration represents the less risky route when wind conditions are not favorable. These findings also help to resolve a long-standing dispute in the literature concerning migration patterns between the US Gulf coast and Mexico, and provide insight into the factors shaping migration strategies of small songbirds migrating across large bodies of water [Current Zoology 60（5）： 653-559, 2014].

关键词： Ecological Barrier Geolocation Gulf of Mexico Tachycineta bicolor Tree swallow Migration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel rice transformation method mediated by low energy ion beam

引用

Rice Science 2002年第2期10卷 6-6页

作者： LI Hong Wu Lifang XIE Chuanxiao Yu Zengliang Key lab of Ion Beam Bioengineering,Inst of Plasma Physics,the Chinese Academy of Sciences,Hefei 230031,China Key lab of Ion Beam Bioengineering Inst of Plasma Physics the Chinese Academy of Sciences Hefei 230031 China

transfer the foreign DNA into rice viaion beam was first reported in 1994in our lab. In this study, we aimedto establish an effieient transformationsystem mediated by low energy ionbeam. Faetors influenced the trans-f... 详细信息

关键词： DNA

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The site conditions of the Guo Shou Jing Telescope

引用

Research in Astronomy and Astrophysics 2012年第7期12卷 772-780页

作者： Song Yao Chao Liu Hao-Tong Zhang Li-Cai Deng Heidi Jo Newberg Yue-Yang Zhang Jing Li Nian Liu Xu Zhou Jeffrey L. Carlin Li Chen Norbert Christlieb Shuang Gao Zhan-Wen Han Jin-Liang Hou Hsu-Tai Lee Xiao-Wei Liu Kai-Ke Pan Hong-Chi Wang Yan Xu Fan Yang Key lab of Optical Astronomy National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Department of Physics Applied Physics and Astronomy Rensselaer Polytechnic Institute 1108th Street Troy NY 12180 USA : School of Physics and Electronic Information China West Normal University Nanchong 637000 China Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Center for Astronomy University of Heidelberg Landesstemwarte K6nigstuhl 12 D-69117Heidelberg Germany Yunnan Astronomical Observatory Chinese Academy of Sciences Kunming 650011 China Institute of Astronomy and Astrophysics Academia Sinica P.O. Box 23-141 Taipei i06 China Department of Astronomy & Kavli Institute of Astronomy and Astrophysics Peking UniversityBeijing 100871 China Apache Point Observatory PO Box 59 Sunspot NM 88349 USA Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China

The weather at the Xinglong Observing Station, where the Guo Shou Jing Telescope （GSJT） is located, is strongly affected by the monsoon climate in north- east China. The LAMOST survey strategy is constrained by these weather patterns. We present statistics on observing hours from 2004 to 2007, and the sky brightness, seeing, and sky transparency from 1995 to 2011 at the site. We investigate effects of the site conditions on the survey plan. Operable hours each month show a strong cor- relation with season： on average there are eight operable hours per night available in December, but only one-two hours in July and August. The seeing and the sky trans- parency also vary with season. Although the seeing is worse in windy winters, and the atmospheric extinction is worse in the spring and summer, the site is adequate for the proposed scientific program of the LAMOST survey. With a Monte Carlo simulation using historical data on the site condition, we find that the available observation hours constrain the survey footprint from 22h to 16h in right ascension; the sky brightness allows LAMOST to obtain a limiting magnitude of V = 19.5 mag with S/N= 10.

关键词： telescopes - site testing - surveys

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses

引用

Journal of Innovative Optical Health Sciences 2023年第6期16卷 13-21页

作者： Nengsong Luo Zengpeng Han Jiaxin Kou Yuxiang Cai Xin Yang Jie Wang Kunzhang Lin Fuqiang Xu Wuhan National laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074P.R.China State Key laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430071P.R.China University of Chinese Academy of Sciences Beijing100049P.R.China Shenzhen Key laboratory of Viral Vectors for Biomedicine Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Productsthe Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsShenzhen518055P.R.China Department of Pathophysiology Key Lab of Neurological Disorder of Education MinistrySchool of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanP.R.China Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions Shenzhen518055P.R.China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai200031P.R.China

Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates,making them powerful tools for determining the structure and function of the complicated neural circuits of the ***,they have some limitations,such as posing high risks to human health and the inability to retrograde trans-synaptic label inputs from genetically-de¯ned starter ***,we established a new retrograde trans-multi-synaptic tracing method through brain-wide rabies virus glycoprotein(RVG)compensation,followed by glycoprotein-deleted rabies virus(RV-△G)infection in specific brain ***,in combination with the avian tumor virus receptor A(TVA)controlled by a cell-type-specific promoter,we found that EnvA-pseudotyped RV-△G can mediate e±cient retrograde trans-multi-synaptic transduction from cell-type-specific starter *** study provides new alternative methods for neuroscience researchers to analyze the input neural networks of rodents and nonhuman primates.

关键词： Rabies virus retrograde tracers trans-multi-synaptic tracing AAV-PHP.eB celltype-specific input neural networks

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：