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Directly targeting BAX for drug disco.ery:Therapeutic opportunities and challenges

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2378-2401页

作者： Zhenwei Zhang Linghui Hou Dan Liu Shenglin Luan Min Huang Linxiang Zhao Key Laboratory of Structure-Based Drugs Design&Disco.ery of Ministry of Education Shenyang Pharmaceutical UniversityShenyang 110016China China Resources Sanjiu Medical&pharmaceutical co. Ltd.Shenzhen 518000China

For over two decades,the development of B-cell lymphoma-2(Bcl-2)family therapeutics has primarily focused on anti-apoptotic proteins,resulting in the first-in-class drugs called BH3 mimetics,especially for Bcl-2 inhibitor *** pro-apoptotic protein Bcl-2-associated X protein(BAX)plays a crucial role as the executioner protein of the mitochondrial regulated cell death,co.tributing to organismal development,tissue homeostasis,and *** dysregulation of BAX is closely associated with the onset and progression of diseases characterized by pathologic cell survival or death,such as cancer,neurodegeneration,and heart *** addition to co.ducting thorough investigations into the physiological modulation of BAX,research on the regulatory mechanisms of small molecules identified through biochemical screening approaches has prompted the identification of functional and potentially druggable binding sites on BAX,as well as diverse all-molecule BAX *** review presents recent advancements in elucidating the physiological and pharmaco.ogical modulation of BAX and in identifying potentially druggable binding sites on ***,it highlights the structural and mechanistic insights into small-molecule modulators targeting diverse binding surfaces or co.formations of BAX,offering a promising avenue for developing next-generation apoptosis modulators to treat a wide range of diseases associated with dysregulated cell death by directly targeting BAX.

关键词： Apoptosis Pro-apoptotic protein BAX Dynamic co.formational activation Small-molecule apoptosis modulators

Human extracellular matrix(ECM)-like co.lagen and its bioactivity

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Regenerative Biomaterials 2024年第4期3卷 14-26页

作者： Hui Zhou Wenwei Li Lixin Pan Tianci Zhu Teng Zhou E.Xiao Qiang Wei State Key Laboratory of Polymer Materials and Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu 610065China Hunan Maybio Bio-pharmaceutical co. LtdChangsha 410000China

co.lagen,the most abundant structural protein in the human extracellular matrix(ECM),provides essential support for tissues and guides tissue *** its widespread use in tissue engineering,there remains uncertainty regarding the optimal selection of co.lagen ***-derived sources pose challenges such as immunogenicity,while the reco.binant system is hindered by diminished *** this study,we hypothesized that human ECM-like co.lagen(hco.)co.ld offer an alternative for tissue *** this study,a facile platform was provided for generating hco. derived from mesenchymal stem cells with a hierarchical structure and biochemical properties resembling native *** results further demonstrated that hco. co.ld facilitate basal biological behaviors of human adipose-derived stem cells,including viability,proliferation,migration and adipocyte-like ***,it co.ld promote cutaneous wound *** to its high similarity to native co.lagen and good bioactivity,hco. holds promise as a prospective candidate for in vitro and in vivo applications in tissue engineering.

关键词： extracellular matrix native-like co.lagen cell-derived matrix hierarchical structure

A sequential co.tinuous flow synthesis and purification process of calcium dibutyryladenosine cyclophosphate

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Chinese Chemical Letters 2024年第2期35卷 402-406页

作者： Liming Cao Maolin Sun Chaoming Liang Lei Yang Yueyue Ma Ruihua Cheng Yanxiong Ke Wei Yu Jinxing Ye Engineering Research Center of pharmaceutical Process Chemistry Ministry of EducationSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China School of Biomedical and pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China Shanghai No.1 Biochemical& pharmaceutical co. Ltd.Shanghai 200080China

Calcium dibutyryladenosine cyclophosphate is a widely used cardiovascular *** traditional batch synthesis process suffers from long reaction times,tedious operations,and unstable ***,a sequential co.tinuous flow synthesis co.bined with a multistage in-line purification process of calcium dibutyryladenosine cyclophosphate was *** acylation reaction was co.pleted in a co.tinuous co.l reactor at 160℃ in 20 *** the high toxic solvent pyridine was replaced by ***,the multistage in-line purification process was integrated into the homemade 3D circular cyclone-type micromixer *** with the membrane phase separators,the residence time of the purification step was 30 *** isolated yield of this sequential co.tinuous process was 92%with 99%purity.

关键词： Flow chemistry Calcium dibutyryladenosine cyclophosphate co.tinuous flow synthesis In-line purification 3D circular cyclone-type micromixer chip

Real-time model co.rection using Kalman filter for Raman-co.trolled cell culture processes

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Chinese Journal of Chemical Engineering 2024年第6期70卷 251-260页

作者： Xiaoxiao Dong Zhuohong He Xu Yan Dong Gao Jingyu Jiao Yan Sun Haibin Wang Haibin Qu pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Hisun Biopharmaceutical co.Ltd. Hangzhou 311404China

Raman spectrosco.y has found extensive use in monitoring and co.trolling cell culture *** this co.text,the prediction accuracy of Raman-based models is of paramount ***,models established with data from manually fed-batch cultures often exhibit poor performance in Raman-co.trolled ***,there is a need for effective methods to rectify these *** objective of this paper is to investigate the efficacy of Kalman filter(KF)algorithm in co.recting Raman-based models during cell ***,partial least squares(PLS)models for different co.ponents were co.structed using data from manually fed-batch cultures,and the predictive performance of these models was ***,various co.rection methods including the PLS-KF-KF method proposed in this study were employed to refine the PLS ***,a case study involving the auto-co.trol of gluco.e co.centration demonstrated the application of optimal model co.rection *** results indicated that the original PLS models exhibited differential performance between manually fed-batch cultures and Raman-co.trolled *** gluco.e,the root mean square error of prediction(RMSEP)of manually fed-batch culture and Raman-co.trolled culture was 0.23 and 0.40 g·L^(-1).With the implementation of model co.rection methods,there was a significant improvement in model performance within Raman-co.trolled *** RMSEP for gluco.e from updating-PLS,KF-PLS,and PLS-KF-KF was 0.38,0.36 and 0.17 g·L^(-1),***,the proposed PLS-KF-KF model co.rection method was found to be more effective and stable,playing a vital role in the automated nutrient feeding of cell cultures.

关键词： Raman spectrosco.y Model co.rection Algorithm Model-predictive co.trol Bioprocess

Gain deeper insights into traditional Chinese medicines using multidimensional chromatography co.bined with chemometric approaches

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Chinese Herbal Medicines 2024年第1期16卷 27-41页

作者： Xinyue Yang Pingping Zeng Jin Wen Chuanlin Wang Liangyuan Yao Min He Department of pharmaceutical Engineering School of Chemical EngineeringXiangtan UniversityXiangtan 411105China Hunan Qianjin Xiangjiang pharmaceutical Joint Stock co. Ltd.Zhuzhou 412000China

Traditional Chinese medicines(TCMs)possess a rich historical background,unique theoretical framework,remarkable therapeutic efficacy,and abundant ***,the modernization and internationalization of TCMs have faced significant obstacles due to their diverse ingredients and unknown *** gain deeper insights into the phytochemicals and ensure the quality co.trol of TCMs,there is an urgent need to enhance analytical ***,two-dimensional(2D)chromatography,which inco.porates two independent separation mechanisms,demonstrates superior separation capabilities co.pared to the traditional one-dimensional(1D)separation system when analyzing TCMs *** the past decade,new techniques have been co.tinuously developed to gain actionable insights from co.plex *** review presents the recent advancements in the application of multidimensional chromatography for the quality evaluation of TCMs,enco.passing 2D-gas chromatography(GC),2D-liquid chromatography(LC),as well as emerging three-dimensional(3D)-GC,3D-LC,and their associated data-processing *** studies highlight the promising potential of multidimensional chromatographic separation for future phytochemical ***,the increased separation capability has resulted in higher-order data sets and greater demands for data-processing *** that multidimensional chromatography is still a relatively nascent research field,further hardware enhancements and the implementation of chemometric methods are necessary to foster its robust development.

关键词： chemometrics multidimensional separation off-line two-dimensional liquid chromatography on-line two-dimensional liquid chromatography quality co.trol traditional chinese medicines two-dimensional gas chromatography

Dammarane-type triterpenoids from Gynostemma longipes and their protective activities on hypoxia-induced injury in PC12 cells

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Chinese Journal of Natural Medicines 2024年第5期22卷 466-480页

作者： LIANG Haizhen CHEN Xiaojuan LI Qi ZHANG Mengmeng LU Pengxin ZHANG Jie SONG Juan ZHANG Tao GUO Baolin MA Baiping Department of pharmaceutical Sciences Beijing Institute of Radiation MedicineBeijing 100850China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China AnKang Chia Tai pharmaceutical co. Ltd.Shaanxi 725000China

Sixteen new dammarane-type triterpenoid saponins(1-16)featuring diverse structural variations in the side chain at C-17,along with twenty-one known analogues(17-37),have been isolated from the rhizomes of Gynostemma longipes ***,a plant renowned for its medicinal and edible *** structural elucidation of these co.pounds was acco.plished through co.prehensive analyses of 1D and 2D NMR and HRMS spectrosco.ic data,supplemented by co.parison with previously reported *** assays on the isolates for their protective effects against hypoxia-induced damage in pheochromocytoma cells(PC12 cells)revealed that nine saponins exhibited significant anti-hypoxic *** investigation into the anti-hypoxia mechanisms of the representative saponins demonstrated that co.pounds 22 and 36 markedly reduced the levels of hypoxia-induced ***,these co.pounds were found to decrease the release of lactate dehydrogenase(LDH)and malondialdehyde(MDA),while increasing the activity of superoxide dismutase(SOD),thereby indicating that the saponins co.ld mitigate hypoxia-induced injuries by ameliorating apoptosis and oxidative *** findings offer substantial evidence for the future utilization and development of ***,identifying dammarane-type triterpenoid saponins as its active anti-hypoxic co.stituents.

关键词： Gynostemma longipes Dammarane-type saponins Anti-hypoxia effect Apoptosis

Differential metabolite analysis of the pharmaco.ynamic differences between different ratios of Dahuang(Radix Et Rhizoma Rhei Palmati)-Taoren(Semen Persicae) herb pair

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Journal of Traditional Chinese Medicine 2024年第3期44卷 515-523页

作者： WANG Xiang HUANG Jianping WANG Yupeng WANG Qilong JING Yajiang ZHANG Gang PENG Liang GAO Jing WANG Hongyan YAN Yonggang Department of Key Laboratory of"Qin pharmaceutical"Research and Development Shaanxi University of Traditional Chinese MedicineXianyang 712046China Department of Yupeng Shaanxi Haitian pharmaceutical co. Ltd.Xianyang 712046China Department of Acupuncture and Tuina Shaanxi University of Traditional Chinese MedicineXianyang 712000China

OBJECTIVE: To explore the material basis of the difference of efficacy of Dahuang(Radix Et Rhizoma Rhei Palmati)-Taoren(Semen Persicae)(DT) drugs with different proportions. METHODS: Samples of different ratios of Dahuang(Radix et Rhizoma Rhei Palnati, DH) to Taoren(Semen Persicae, TR)(Group A 1∶1, B 2∶3, C 3∶2) were analyzed based on gas chromatography time-of-flight mass spectrometry untargeted metabolomics technique. RESULTS: A total of 240 primary metabolites were detected. Forty-one differential metabolites involved nine differential metabolic pathways, of which four were closely related to the efficacy of DT in the treatment of heat and blood stasis syndrome. These pathways included the biosynthesis of amino acid(phenylalanine tyrosine and tryptophan), flavonoids, unsaturated fatty acids, and the glyco.ysis/glyco.enesis pathway. co.CLUSION: There are significant differences in the efficacy of different ratios of DT drugs, and their optimal ratio for the treatment of heat and blood stasis syndrome should be 1∶1.

关键词： metabolomics metabolic networks and pathways differential metabolites Dahuang(Radix Et Rhizoma Rhei Palmati) Taoren(Semen Persicae) kernel drug pairs

Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA_(3)-inhibited reactive oxygen species detoxification and abscisic acid signaling

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Journal of Integrative Agriculture 2024年第9期23卷 2989-3011页

作者： Guoling Guo Haiyan Zhang Weiyu Dong Bo Xu Youyu Wang Qingchen Zhao Lun Liu Xiaomei Tang Li Liu Zhenfeng Ye Wei Heng Liwu Zhu Bing Jia School of Horticulture Anhui Agricultural UniversityHefei 230036China Anhui Wanbang pharmaceutical Technology co. Ltd.Hefei 230036China Science and Technology Department Anhui Agricultural UniversityHefei 230036China Hunan Suncas Agriculture Science co. Ltd.Changsha 410016China

Drought stress is a devastating natural disaster driven by the co.tinuing intensification of global warming,which seriously threatens the productivity and quality of several horticultural crops,including ***(GAs)play crucial roles in plant growth,development,and responses to drought *** studies have shown significant reductions of GA levels in plants under drought stress;however,our understanding of the intrinsic regulation mechanisms of GA-mediated drought stress in pear remains very ***,we show that drought stress can impair the accumulation of bioactive GAs(BGAs),and subsequently identified PbrGA2ox1 as a chloroplast-localized GA deactivation *** gene was significantly induced by drought stress and abscisic acid(ABA)treatment,but was suppressed by GA_(3)***2ox1-overexpressing transgenic tobacco.plants(Nico.iana benthamiana)exhibited enhanced tolerance to dehydration and drought stresses,whereas knock-down of PbrGA2ox1 in pear(Pyrus betulaefolia)by virus-induced gene silencing led to elevated drought *** plants were hypersensitive to ABA,and had a lower BGAs co.tent,enhanced reactive oxygen species(ROS)scavenging ability,and augmented ABA accumulation and signaling under drought stress co.pared to wild-type ***,the opposite effects were observed with PbrGA2ox1 silencing in ***,exogenous GA_(3)treatment aggravated the ROS toxic effect and restrained ABA synthesis and signaling,resulting in the co.promised drought tolerance of *** summary,our results shed light on the mechanism by which BGAs are eliminated in pear leaves under drought stress,providing further insights into the mechanism regulating the effects of GA on the drought tolerance of plants.

关键词： pear gibberellin drought stress PbrGA2ox1 reactive oxygen species abscisic acid

Asymmetric Synthesis of Dihydrospirotryprostatin B via a Silica Gel-Mediated Cyclization of Tryptamine-Ynamide

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Chinese Journal of Chemistry 2024年第12期42卷 1355-1359页

作者： Changhong Han Lu Yang Kaizong Yao Sen Zhang Jiayue Fu Hanyang Sun Hongsheng Xu Bin Lin Maosheng Cheng Yongxiang Liu Key Laboratory of Structure:Based Drug Design and Disco.ery of Ministry of Education Shenyang Pharmaceutical UniversityShenyangLiaoning 110016China Wuya co.lege of Innovation Shenyang Pharmaceutical UniversityShenyangLiaoning 110016China Institute of Drug Research in Medicine Capital of China BenxiLiaoning 117000China Zhejiang Shapuaisi pharmaceutical co. LtdPinghuZhejiang 314299China

co.prehensive Summary An asymmetric synthesis of dihydrospirotryprostatin B was achieved in 15 steps(8 purifications)from *** main feature of our synthetic strategy is the efficient co.struction of spirocyclic oxindole intermediate co.taining a chiral quaternary carbon center,involving the silica gel-mediated cyclization of tryptamine-ynamide and oxidation under neat co.ditions.

关键词： Asymmetric synthesis Alkaloids Dihydrospirotryprostatin B Tryptamine-ynamide Cross-co.pling Silica gel-mediated cyclization Oxidation