检索结果-南通市图书馆

The excessive response： a preparation for harder conditions

在线全文

学校读者我要写书评

暂无评论

Protein & cell 2017年第10期8卷 707-710页

作者： Yaguang Ren Chao Zhang Translational Medical center for stem cell Therapy and Institute for Regenerative Medicine Shanghai East Hospital Shangha Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shangha 200433 China

Reactive oxygen species （ROS） are kinds of reactive chemicals mainly formed in mitochondria as byproduct of normal metabolism of oxygen （Balaban et al., 2005）. Because of the high reactiveness they usually react with proteins, lipid, and nuclear acids （Labuschagne and Brenkman, 2013; Landolfo et al., 2008）, and in theory may participate in every aspects of cellular metabolism.

关键词：

Progress in clinical trials of stem cell therapy for cerebral palsy

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration research 2021年第7期16卷 1377-1382页

作者： Zhong-Yue Lv Ying Li Jing Liu stem cell Clinical research center National Joint Engineering LaboratoryRegenerative Medicine CenterThe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of stem cell and Precision Medicine DalianLiaoning ProvinceChina

Cerebral palsy is the most common disease in children associated with lifelong disability in many *** research has demonstrated that traditional physiotherapy and rehabilitation therapies cannot alone cure cerebral *** cell transplantation is an emerging therapy that has been applied in clinical trials for a variety of neurological diseases because of the regenerative and unlimited proliferative capacity of stem *** this review, we summarize the design schemes and results of these clinical *** findings reveal great differences in population characteristics, stem cell types and doses, administration methods, and evaluation methods among the included clinical ***, we also assess the safety and efficacy of these clinical *** anticipate that our findings will advance the rational development of clinical trials of stem cell therapy for cerebral palsy and contribute to the clinical application of stem cells.

关键词： adverse events brain cell transplantation central nervous system cerebral palsy clinical trials plasticity regeneration stem cell

H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2019年第3期17卷 297-304页

作者： Xiaolong Chen Youqiong Ye Liang Gu Jin Sun Yanhua Du Wen-Ju Liu Wei Li Xiaobai Zhang Cizhong Jiang Institute of Translational research Tongji HospitalSchool of Life Sciences and TechnologyShanghai Key Laboratory of Signaling and Disease ResearchTongji UniversityShanghai 200092China tongji University Library Tongji UniversityShanghai 200092China research center of stem cells and Ageing Tsingtao Advanced Research InstituteTongji UniversityTsingtao 266071China

Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic ***,the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain ***,we compared the changes in gene expression,as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural *** find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles,whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells.H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells(NSCs)to the intermediate progenitor cells to the glial *** contrast,H3K9ac signal fails to identify the differentiation path,although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia,***,our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development.

关键词： Nucleosome Histone modification Neural stem cell Neuron Glia

A neurovascular unit-on-a-chip:culture and differentiation of human neural stem cells in a three-dimensional microfluidic environment

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration research 2022年第10期17卷 2260-2266页

作者： Wen-Juan Wei Ya-Chen Wang Xin Guan Wei-Gong Chen Jing Liu stem cell Clinical research center National Joint Engineering LaboratoryRegenerative Medicine Centerthe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of stem cell and Precision Medicine DalianLiaoning ProvinceChina

Biological studies typically rely on a simple monolayer cell culture,which does not reflect the complex functional characteristics of human tissues and organs,or their real response to external *** technology has advantages of high-throughput screening,accurate control of the fluid velocity,low cell consumption,long-term culture,and high *** combining the multipotential differentiation of neural stem cells with high throughput and the integrated characteristics of microfluidic technology,an in vitro model of a functionalized neurovascular unit was established using human neural stem cell-derived neurons,astrocytes,oligodendrocytes,and a functional microvascular *** model comprises a multi-layer vertical neural module and vascular module,both of which were connected with a syringe *** provides controllable conditions for cell inoculation and nutrient supply,and simultaneously simulates the process of ischemic/hypoxic injury and the process of inflammatory factors in the circulatory system passing through the blood-brain barrier and then acting on the nerve tissue in the *** in vitro functionalized neurovascular unit model will be conducive to central nervous system disease research,drug screening,and new drug development.

关键词： (neural)differentiation astrocyte blood-brain barrier brain microvascular endothelial cells central nervous system microfluidics neural stem cells neuron neurovascular unit oligodendrocyte organ-on-a-chip

Progress in studies on the induction and differentiation of embryonic stem cells

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2005年第5期48卷 419-423页

作者： YANG Yinxiang LIU Daqing PEI Xuetao research center of stem cell and Regenerative Medicine Institute of Blood Transfusion Academy of Military Medical Sciences Beijing 100850 China Research Center of Stem Cell and Regenerative Medicine Institute of Blood Transfusion Academy of Military Medical Sciences Beijing 100850 China Research Center of Stem Cell and Regenerative Medicine Institute of Blood Transfusion Academy of Military Medical Sciences Beijing 100850 China

Embryonic stem cells (ESCs), which are isolated from the inner cell mass of the blastocyst stage embryo, have the potential to give rise to an entire organism and to generate every body cell type. Much improvement has been made in the field of induction and differentia-tion of ESCs during the last two years, such as the ESCs differentiation into germ cells (2003) and the cloning of human ESCs (2004), both of which were chosen respectively as one of the top ten achievements evaluated by academic journals. Great attention was also paid to the research of the new genes which could maintain ESCs in the undifferentiated state and the research of the induction and differentiation of ESCs.

关键词： embryonic stem cells (ESCs), induction, differentiation.

Activation of the Melanocortin-4 receptor signaling byα-MSH stimulates nervedependent mouse digit regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cell Regeneration 2021年第1期10卷 215-225页

作者： Hanqian Xu Hailin Zhang Yanqing Fang Huiran Yang Ying Chen Chao Zhang Gufa Lin Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Orthopaedic Department of Tongji HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina stem cell Institute University of MinnesotaMinneapolisMN 55455USA Translational Medical center for stem cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalShanghai Key Laboratory of Signaling and Disease ResearchFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina

Background:Expression of Mc4r in peripheral organs indicates it has broader roles in organ homeostasis and ***,the expression and function of Mc4r in the mouse limb and digit has not been fully *** previous work showed that Mc4r−/−mice fail to regenerate the digit,but whether activation of MC4R signaling could rescue digit regeneration,or stimulate proximal digit regeneration is not ***:We analyzed the expression dynamics of Mc4r in the embryonic and postnatal mouse limb and digit using the Mc4r-gfp *** found that Mc4r-GFP is mainly expressed in the limb nerves,and in the limb muscles that are undergoing secondary *** of Mc4r-GFP in the adult mouse digit is restricted to the nail *** also examined the effect ofα-MSH on mouse digit *** found that administration ofα-MSH in the Mc4r+/−mice rescue the delayed regeneration of distal digit tip.α-MSH could rescue distal digit regeneration in denervated *** addition,α-MSH could stimulate regeneration of the proximally amputated digit,which is ***:Mc4r expression in the mouse limb and digit is closely related to nerve tissues,andα-MSH/MC4R signaling has a neurotrophic role in mouse digit tip regeneration.

关键词： MC4R Mc4r-gfp α-MSH Digit regeneration Distal Proximal Denervation Neurotrophic function

Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1237-1245页

作者： Gongchen Zhang Chenyu Zhu Xiaohan Chen Jifang Yan Dongyu Xue Zixuan Wei Guohui Chuai Qi Liu Translational Medical center for stem cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China

Base editing technology is being increasingly applied in genome engineering,but the current strategy for designing guide RNAs(gRNAs)relies substantially on empirical experience rather than a dependable and efficient in silico ***,the pleiotropic effect of base editing on disease treatment remains unexplored,which prevents its further clinical ***,we presented BExplorer,an integrated and comprehensive computational pipeline to optimize the design of gRNAs for 26 existing types of base editors in *** BExplorer,we described its results for two types of mainstream base editors,BE3 and ABE7.10,and evaluated the pleiotropic effects of the corresponding base editing *** revealed 524 and 900 editable pathogenic single nucleotide polymorphism(SNP)loci in the human genome together with the selected optimized gRNAs for BE3 and ABE7.10,*** addition,the impact of 707 edited pathogenic SNP loci following base editing on 131 diseases was systematically explored by revealing their pleiotropic effects,indicating that base editing should be carefully utilized given the potential pleiotropic ***,the systematic exploration of optimized base editing gRNA design and the corresponding pleiotropic effects with BExplorer provides a computational basis for applying base editing in disease treatment.

关键词： Gene editing Base editing Pleiotropy CRISPR/Cas9 gRNAdesign

Human umbilical cord-derived mesenchymal stem cells alleviate oxidative stress-induced islet impairment via the Nrf2/HO-1 axis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular cell Biology 2023年第5期15卷 38-51页

作者： Peng Liu Baige Cao Yang Zhou Huina Zhang Congrong Wang Shanghai Diabetes Institute Department of Endocrinology and MetabolismShanghai Key Laboratory of Diabetes MellitusShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200233China Department of Endocrinology&Metabolism Shanghai Fourth People’s HospitalSchool of MedicineTongji UniversityShanghai 200434China Translational Medical center for stem cell Therapy Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200092China stem cell Translational research center Tongji HospitalSchool of MedicineTongji UniversityShanghai 200065China

Hyperglycaemia-induced oxidative stress may disrupt insulin secretion andβ-cell survival in diabetes mellitus by overproducing reactive oxygen *** umbilical cord-derived mesenchymal stem cells(hUC-MSCs)exhibit antioxidant ***,the mechanisms by which hUC-MSCs protectβ-cells from high glucose-induced oxidative stress remain *** this study,we showed that intravenously injected hUC-MSCs engrafted into the injured pancreas and promoted pancreaticβ-cell function in a mouse model of type 1 diabetes *** in vitro study revealed that hUC-MSCs attenuated high glucoseinduced oxidative stress and preventedβ-cell impairment via the Nrf2/HO-1 signalling ***2 knockdown partially blocked the anti-oxidative effect of hUC-MSCs,resulting inβ-cell decompensation in a high-glucose ***,these findings provide novel insights into how hUC-MSCs protectβ-cells from high glucose-induced oxidative stress.

关键词： type 1 diabetes β-cell protection mesenchymal stem cells oxidative stress Nrf2

Genome transfer for the prevention of female infertility caused by maternal gene mutation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2020年第6期47卷 311-319页

作者： Dandan Bai Jin Sun Yanping Jia Jiqing Yin Yalin Zhang Yanhe Li Rui Gao Xiling Du Kunming Li Jiaming Lin Zhifen Tu Yu Wang Jiaping Pan Shanshan Liang Yi Guo Jingling Ruan Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Fengchao Wang Xiaoming Teng Wenqiang Liu Shaorong Gao Clinical and Translational research center of Shanghai First Maternity and Infant Hospital.Shanghai Key Laboratory of Signaling and Disease research Frontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai200092China National Institute of Biological Sciences.NIBS.Beijing 102206China

Poor oocyte quality is associated with early embryo developmental arrest and *** gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female ***,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains ***,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female *** first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic *** next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused *** finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live ***,those pups grow to adulthood and show normal ***,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation.

关键词： Infertility Oocytes Zar1 Maternal-zygotic transition ZGA Spindle transfer Genome transfer