检索结果-南通市图书馆

regenerative treatment of ophthalmic diseases with stem cells:Principles,progress,and challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Ophthalmology Practice and Research 2024年第2期4卷 52-64页

作者： Yifei Niu Junfeng Ji Ke Yao Qiuli Fu Eye Center of the Second Affiliated Hospital Zhejiang University School of MedicineHangzhouZhejiang ProvinceChina Zhejiang Provincial Key Lab of Ophthalmology HangzhouZhejiang ProvinceChina Center of Stem Cell and regenerative medicine School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Zhejiang Provincial Key laboratory of tissue engineering and regenerative medicine HangzhouChina

Background:Degenerate eye disorders,such as glaucoma,cataracts and age-related macular degeneration(AMD),are prevalent causes of blindness and visual impairment *** eye disorders,including limbal stem cell deficiency(LSCD),dry eye diseases(DED),and retinitis pigmentosa(RP),result in symptoms such as ocular discomfort and impaired visual function,significantly impacting quality of *** therapies are limited,primarily focus on delaying disease progression,while emerging stem cell therapy directly targets ocular tissues,aiming to restore ocular function by reconstructing ocular *** text:The utilization of stem cells for the treatment of diverse degenerative ocular diseases is becoming increasingly significant,owing to the regenerative and malleable properties of stem cells and their functional ***,stem cell therapy for ophthalmopathy involves various cell types,such as embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),mesenchymal stem cells(MSCs),and retinal progenitor cells(RPCs).In the current article,we will review the current progress regarding the utilization of stem cells for the regeneration of ocular tissue covering key eye tissues from the cornea to the *** therapies aim to address the loss of functional cells,restore damaged ocular tissue and or in a paracrine-mediated *** also provide an overview of the ocular disorders that stem cell therapy is targeting,as well as the difficulties and opportunities in this ***:Stem cells can not only promote tissue regeneration but also release exosomes to mitigate inflam-mation and provide neuroprotection,making stem cell therapy emerge as a promising approach for treating a wide range of eye disorders through multiple mechanisms.

关键词： Stem cell Ocular diseases Induced pluripotent stem cells Mesenchymal stem cells Cataracts Glaucoma Diabetic retinopathy Retinal degenerative diseases

A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.J.Dijkstra C.Mota J.Bauer H.Ferreira Rui L.Reis N.Neves S.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

Biomaterials and emerging technologies for tissue engineering and in vitro models

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2024年第3期7卷 237-239页

作者： J.Miguel Oliveira Rui L.Reis 3B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate laboratory 4806-909 Braga/GuimarãesPortugal

The latest advances in the field of biomaterials have opened new avenues for scientific breakthroughs in tissue engineer-ing which greatly contributed for the successful translation of tissue engineering products into the market/***-materials are easily processed to become similar to natural extracellular matrix,making them ideal temporary supports for mimicking the three-dimensional(3D)microenvironment required for maintaining the adequate cell/tissue functions both in vitro and in vivo^([1]).

关键词： breakthrough biomaterials engineer

Hierarchical Spinning of Janus Textiles with Anisotropic Wettability for Wound Healing

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 97-105页

作者： Han Zhang Lingyu Sun Jiahui Guo Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjingJiangsu 210096China Oujiang laboratory(Zhejiang Lab for regenerative medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang 325001China

Wound healing and tissue repair are recognized as basic human health problems *** to accelerate the reparative process are focused on developing functional wound ***,we present novel Janus textiles with anisotropic wettability from hierarchical microfluidic spinning for wound *** hydrophilic hydrogel microfibers from microfluidics are woven into textiles for freeze-drying treatment,followed by the deposition of electrostatic spinning nanofibers composed of hydrophobic polylactic acid(PLA)and silver *** electrospun nanofiber layer can be well coupled with the hydrogel microfiber layer to generate Janus textiles with anisotropic wettability due to the roughness of the hydrogel textile surface and the incomplete evaporation of PLA solution when reaching the *** wound treatment with the hydrophobic PLA side contacting the wound surface,the wound exudate can be pumped from the hydrophobic to the hydrophilic side based on the wettability differential derived drainage *** this process,the hydrophobic side of the Janus textile can prevent excess fluid from infiltrating the wound again,preventing excessive moisture and preserving the breathability of the *** addition,the silver nanoparticles contained in the hydrophobic nanofibers could impart the textiles with good antibacterial effect,which further promote the wound healing *** features indicate that the described Janus fiber textile has great application potential in the field of wound treatment.

关键词： silver spinning healing

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

regenerative Biomaterials 2024年第3期3卷 1-29页

作者： Peiyao Xu Ranjith Kumar Kankala Shibin Wang Aizheng Chen Institute of Biomaterials and tissue engineering Huaqiao UniversityXiamenFujian 361021PR China Fujian Provincial Key laboratory of Biochemical Technology(Huaqiao University) XiamenFujian 361021PR China

Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering,the clinical transformation of these scaffolds remained a big challenge because of the difficulty of simulating native organs/tissues'*** a kind of natural tissue-derived biomaterials,decellularized extracellular matrix(dECM)-based scaffolds have gained attention due to their unique biomimetic properties,providing a specific microenvironment suitable for promoting cell proliferation,migration,attachment and regulating *** medical applications of dECM-based scaffolds have addressed critical challenges,including poor mechanical strength and insufficient *** promoting the reconstruction of damaged tissues or organs,dif-ferent types of dECM-based composite platforms have been designed to mimic tissue microenvironment,including by integrating with natural polymer or/and syntenic polymer or adding bioactive *** this review,we summarized the research progress of dECM-based composite scaffolds in regenerative medicine,highlighting the critical challenges and future perspectives related to the medical application of these composite materials。

关键词： decellularized extracellular matrix polymer bioactive factors composites tissue engineering

NIR-switchable local hydrogen generation by tandem bimetallic MOFs nanocomposites for enhanced chemodynamic therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

regenerative Biomaterials 2024年第1期3卷 58-68页

作者： Jun Zhong Xiang Zheng Yuan Wen Yuewei Li Jianting Zhang Ranjith Kumar Kankala Shibin Wang Aizheng Chen Institute of Biomaterials and tissue engineering Huaqiao UniversityXiamen 361021China Fujian Provincial Key laboratory of Biochemical Technology Huaqiao UniversityXiamen 361021China Fujian Provincial Key laboratory of Biomass Low-Carbon Conversion Huaqiao UniversityXiamen 361021China

The inadequate quantity of hydrogen peroxide(H_(2)O_(2))in cancer cells promptly results in the constrained success of chemodynamic therapy(CDT).Significant efforts made throughout the years;nevertheless,researchers are still facing the great challenge of designing a CDT agent and securing H_(2)O_(2) supply within the tumor *** this study,taking advantage of H_(2)O_(2) level maintenance mechanism in cancer cells,a nanozyme-based bimetallic metal-organic frameworks(MOFs)tandem reactor is fabricated to elevate intracellular H_(2)O_(2) levels,thereby enhancing *** addition,under nearinfrared excitation,the upconversion nanoparticles(UCNPs)loaded into the MOFs can perform photocatalysis and generate hydrogen,which increases cellular susceptibility to radicals induced from H_(2)O_(2),inhibits cancer cell energy,causes DNA damages and induces tumor cell apoptosis,thus improving CDT therapeutic efficacy *** proposed nanozyme-based bimetallic MOFs-mediated CDT and UCNPs-mediated hydrogen therapy act as combined therapy with high efficacy and low toxicity.

关键词： chemodynamic therapy nanozyme bimetallic MOFs gas therapy

Living Chinese Herbal Scaffolds from Microfluidic Bioprinting for Wound Healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 107-118页

作者： Xiaocheng Wang Jinxuan Jia Mengying Niu Wenzhao Li Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Oujiang laboratory(Zhejiang Lab for regenerative medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhouZhejiang 325001China State Key laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Chemistry and Biomedicine Innovation Center Nanjing UniversityNanjing 210023China

Biological scaffolds have been widely employed in wound healing applications,while their practical efficiency is compromised by insufficient oxygen delivery to the 3-dimensional constructs and inadequate nutrient supply for the long-term healing ***,we present an innovative living Chinese herbal scaffold to provide a sustainable oxygen and nutrient supply for promoting wound *** a facile microfluidic bioprinting strategy,a traditional Chinese herbal medicine(Panax notoginseng saponins[PNS])and a living autotrophic microorganism(microalgae Chlorella pyrenoidosa[MA])were successfully encapsulated into the *** encapsulated PNS could be gradually released from the scaffolds,which promoted cell adhesion,proliferation,migration,and tube formation in *** addition,benefiting from the photosynthetic oxygenation of the alive MA,the obtained scaffolds would produce sustainable oxygen under light illumination,exerting a protective effect against hypoxia-induced cell *** on these features,we have demonstrated through in vivo experiments that these living Chinese herbal scaffolds could efficiently alleviate local hypoxia,enhance angiogenesis,and thereby accelerate wound closure in diabetic mice,indicating their great potential in wound healing and other tissue repair applications.

关键词： healing wound protective

Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2024年第3期7卷 307-319页

作者： Sen Wang Siqi Yao Na Pei Luge Bai Zhiyan Hao Dichen Li Jiankang He J.Miguel Oliveira Xiaoyan Xue Ling Wang Xinggang Mao State Key laboratory for Manufacturing System engineering Xi’an Jiaotong UniversityXi’an 710054China School of Mechanical engineering Xi’an Jiaotong UniversityXi’an 710054China NMPA Key laboratory for Research and Evaluation of Additive Manufacturing Medical Devices Xi’an Jiaotong UniversityXi’an 710054China 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate laboratory 4806-909 Braga/Guimar˜aesPortugal Department of Pharmacology School of PharmacyFourth Military Medical UniversityXi’an 710032China Department of Neurosurgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor ***,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor *** study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I *** also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and ***,it compares two-dimensional(2D)and 3D *** comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D ***,the two models yielded significantly different cell ***,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D *** results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture *** developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

关键词： Hypoxia Glioma Three-dimensional glioma model In vitro

Mesenchymal stem cell-derived exosomes: Versatile nanomaterials for skin wound treatment

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第4期17卷 2836-2856页

作者： Yuzhen Xiao Hexi Li Junhui Zhang Songyun Yang Chunsen Zhang Yizhou Huang Xin Tang Huiqi Xie Department of Orthopedic Surgery and Orthopedic Research Institute Laboratory of Stem Cell and Tissue EngineeringState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China West China School of Basic Medical Sciences and Forensic medicine Sichuan UniversityChengdu 610041China West China School of Public Health and West China Fourth Hospital Sichuan UniversityChengdu 610041China Sports medicine Center West China HospitalSichuan UniversityChengdu 610041China

Accumulating studies reveal that mesenchymal stem cells(MSCs)promote skin wound healing mainly through the paracrine ***,one of the crucial paracrine mediators in wound healing,are cell-derived nanosized membranous vesicles containing diverse bioactive *** the potent ability of modulating skin cell behaviors,MSC-derived exosomes(MSC-Exos)are regarded as a promising nanomaterial for regenerative wound *** hostile conditions,MSC-Exos are efficient in protecting skin cells from severe damage and restoring their *** to recent studies,MSC-Exos possess remarkable pro-healing effects in a variety of skin wounds,typically resulting in increased wound closure,inhibited scar tissue formation,and better restoration of skin *** further enhance the therapeutic potential of MSC-Exos,the development of applicable pretreatment strategies and the optimization of exosome delivery are under intensive ***,we summarize current research progress of MSC-Exos for skin wound treatment,with an emphasis on the biological effects of these nanovesicles,the repair mechanisms,and future challenges in clinical translation.

关键词： exosomes mesenchymal stem cells diabetic wounds burn wounds scar wound healing