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Cardiac cell therapy and tissue Engineered with Autologous Bone Marrow Mesenchymal cells Improve Myocardial Perfusion. An Evaluation by Pinhole Gated-SPECT

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生物医学工程学杂志 2005年第S1期22卷 15-16页

作者： Nguyen TRAN Pierre-Yves MARIE Philippe FRANKEN Jean-Fran·ois STOLTZ Jean-Pierre VILLEMOT Laboratory of Surgery School Faculty of Medicine-NancyFrance Department of cell therapy and tissue engineering UMR-CNRS7563 Department of Nuclear Medicine-EA344 CHU-NancyFrance Department of Nuclear Medicine AZ-VUB-BrusselsBelgium

Corneal re-innervation following refractive surgery treatments

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Neural Regeneration Research 2019年第4期14卷 557-565页

作者： Francisco Bandeira Nur Zahira Yusoff Gary Hin-Fai Yam Jodhbir Singh Mehta tissue engineering and Stem cell group Singapore Eye Research Institute Federal University of S?o Paulo S?o Gon?alo Eye Hospital Eye-ACP Duke-NUS Graduate Medical School Department of Ophthalmology Yong Loo Lin School of Medicine National University of Singapore Singapore National Eye Centre School of Material Science and engineering Nanyang Technological University

Laser refractive surgery is one of the most performed surgical procedures in the world. Although regarded safe and efficient, it has side effects. All of the laser based refractive surgical procedures invoke corneal nerve injury to some degree. The impact of this denervation can range from mild discomfort to neurotrophic corneas. Currently, three techniques are widely used for laser vision correction: small incision lenticule extraction, laser-assisted keratomileusis in situ and photorefractive keratotomy. Each of these techniques affects corneal innervation differently and has a different pattern of nerve regeneration. The purpose of this review is to summarize the different underlying mechanisms for corneal nerve injury and compare the different patterns of corneal reinnervation.

关键词： photorefractive keratotomy small incision manual lenticule extraction laser-assisted keratomileusis in situ refractive surgery in vivo confocal microscopy corneal sensation corneal nerve

Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells

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Bone Research 2018年第4期6卷 393-407页

作者： Wenjia Liu Liqiang Zhang Kun Xuan Chenghu Hu Shiyu Liu Li Liao Bei Li Fang Jin Songtao Shi Yan Jin MS-State Key Laboratory & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases Center for Tissue EngineeringSchool of StomatologyFourth Military Medical UniversityXi’anChina Xi’an Institute of tissue engineering and Regenerative Medicine Xi’anChina Department of Anatomy and cell Biology School of Dental MedicineUniversity of PennsylvaniaPhiladelphiaPAUSA

Mutations in the liver/bone/kidney alkaline phosphatase(Alpl) gene cause hypophosphatasia(HPP) and early-onset bone dysplasia,suggesting that this gene is a key factor in human bone development. However, how and where Alpl acts in bone ageing is largely unknown. Here, we determined that ablation of Alpl induces prototypical premature bone ageing characteristics, including bone mass loss and marrow fat gain coupled with elevated expression of p16INK4A(p16) and p53 due to senescence and impaired differentiation in mesenchymal stem cells(MSCs). Mechanistically, Alpl deficiency in MSCs enhances ATP release and reduces ATP hydrolysis. Then, the excessive extracellular ATP is, in turn, internalized by MSCs and causes an elevation in the intracellular ATP level, which consequently inactivates the AMPKα pathway and contributes to the cell fate switch of MSCs. Reactivating AMPKα by metformin treatment successfully prevents premature bone ageing in Alpl+/-mice by improving the function of endogenous *** results identify a previously unknown role of Alpl in the regulation of ATP-mediated AMPKα alterations that maintain MSC stemness and prevent bone ageing and show that metformin offers a potential therapeutic option.

关键词： Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells ATP

Mesenchymal stromal cells attenuate post-stroke infection by preventing caspase-1-dependent splenic marginal zone B cell death

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Signal Transduction and Targeted therapy 2021年第3期6卷 590-592页

作者： Yinong Huang Jiancheng Wang Xiaofan Lai Yuan Qiu Jianye Cai Yuanchen Ma Yilin Liu Wei Qiu Zhengqi Lu Andy Peng Xiang Department of Neurology The Third Affiliated HospitalSun Yat-Sen UniversityGuangzhouChina Center for Stem cell Biology and tissue engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-Sen UniversityGuangzhouChina The Seventh Affiliated Hospital Sun Yat-sen UniversityShenzhenChina

Dear Editor,Spontaneous infection is one of the most common complications of acute ischemic stroke(AIS)and leads to increased morbidity and mortality in stroke ***,current interventions fail to improve clinical outcomes in these *** stroke-induced immunodeficiency is thought to contribute to the risk of infection,1 we hypothesized that mesenchymal stromal cell(MSC)therapy,which harnesses potent immunomodulatory capacities,could be a potential ***,we show that intravenously administered MSCs preferentially migrate to the marginal zone(MZ)of the spleen,preserve the injured MZ B cells and ameliorate post-stroke infection and mortality in the mouse middle cerebral artery occlusion(MCAO)model of AIS.

关键词： infection mortality spleen

Embryoid bodies formation and differentiation from mouse embryonic stem cells in collagen/Matrigel scaffolds

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Journal of Genetics and Genomics 2010年第7期37卷 451-460页

作者： Jin Zhou Ye Zhang Qiuxia Lin Zhiqiang Liu Haibin Wang Cuimi Duan Yanmeng Wang Tong Hao Kmwu Wu Changyong Wang Department of tissue engineering Institute of Basic Medical Sciences and Tissue Engineering Research Center Academy of Military Medical Sciences Beijing 100850 China Department of Hematology and Oncology General Hospital of the Artillery Force Second Beijing 100850 China

Embryonic stem （ES） cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation *** main catalyst for ES cells differentiation is the growth into embryoid bodies （EBs）,which are utilized widely as the trigger of in vitro *** this study,a novel method for generating EBs from mouse ES cells through culture in collagen/Matrigel scaffolds was successfully *** single ES cells were seeded in three dimensional collagen/Matrigel scaffolds,they grew into aggregates gradually and formed simple EBs with circular *** 7 days＇ culture,they formed into cystic EBs that would eventually differentiate into the three embryonic germ *** of the EBs in terms of morphology and potential to differentiate indicated that they were typical in structure and could generate various cell types;they were also able to form into tissue-like ***,with introduction of ascorbic acid,ES cells differentiated into cardiomyocytes efficiently and started contracting synchronously at day *** results demonstrated that collagen/Matrigel scaffolds supported EBs formation and their subsequent differentiation in a single three dimensional environment.

关键词： embryonic stem (ES) cells embryoid bodies (EBs) differentiation collagen/Matrigel scaffolds model

Novel advancements in threedimensional neural tissue engineering and regenerative medicine

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Neural Regeneration Research 2015年第3期10卷 352-354页

作者： Richard J.Mc Murtrey Institute of Neural Regeneration & tissue engineering Institute of Biomedical engineering Department of Engineering ScienceOld Road Campus Research BuildingUniversity of Oxford

Neurological diseases and injuries present some of the great- est challenges in modern medicine, often causing irrevers- ible and lifelong burdens in the people whom they afflict. Conditions of stroke, traumatic brain injury, spinal cord injury, and neurodegenerative diseases have devastating con- sequences on millions of people each year, and yet there are currently no therapies or interventions that can repair the structure of neural circuits and restore neural tissue function in the brain and spinal cord. Despite the challenges of over- coming these limitations, there are many new approaches under development that hold much promise. Neural tissue engineering aims to restore and influence the function of damaged or diseased neural tissue generally through the use of stem cells and biomaterials. Many types of biomaterials may be implemented in various designs to influence the survival, differentiation, and function of developing stem cells, as well as to guide neurite extension and morphological architecture of cell cultures. Such designs may aim to reca- pitulate the cellular interactions, extracellular matrix char- acteristics, biochemical factors, and sequences of events that occur in neurodevelopment, in addition to supporting cell survival, differentiation, and integration into innate neural tissue.

关键词： Novel advancements in threedimensional neural tissue engineering and regenerative medicine

Important parameters in plasma jets for the production of RONS in liquids for plasma medicine: A brief review

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Frontiers of Chemical Science and engineering 2019年第2期13卷 238-252页

作者： Anna Khlyustova Cédric Labay Zdenko Machala Maria-Pau Ginebra Cristina Canal Biomaterials Biomechanics and Tissue Engineering GroupDepartment of Materials Science and MetallurgyUniversitat Politecnica de CatalunyaBarcelona 08019Spain Research centre in Multiscale Science and engineering Universitat Politecnica de CatalunyaBarcelona 08019Spain Faculty of Mathematics Physics and InformaticsComenius UniversityBratislava 84248Slovakia

Reactive oxygen and nitrogen species (RONS) are among the key factors in plasma medicine. They are generated by atmospheric plasmas in biological fluids, living tissues and in a variety of liquids. This ability of plasmas to create a delicate mix of RONS in liquids has been used to design remote or indirect treatments for oncological therapy by treating biological fluids by plasmas and putting them in contact with the tumour;Documented effects include selective cancer cell toxicity, even though the exact mechanisms involved are still under investigation. However, the "right" dose for suitable therapeutical activity is crucial and still under debate. The wide variety of plasma sources hampers comparisons. This review focuses on atmospheric pressure plasma jets as the most studied plasma devices in plasma medicine and compiles the conditions employed to generate RONS in relevant liquids and the concentration ranges obtained. The concentrations of H2O2, NO2^-, NO3^- and short-lived oxygen species are compared critically to provide a useful overview for the reader.

关键词： atmospheric plasma jets liquids ROS RNS plasma-dose

Urine-derived stem cells:applications in skin,bone and articular cartilage repair

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Burns & Trauma 2021年第1期9卷 95-108页

作者： Wenqian Zhang Jungen Hu Yizhou Huang Chenyu Wu Huiqi Xie Laboratory of Stem cell and tissue engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China

As an emerging type of adult stem cell featuring non-invasive acquisition,urine-derived stem cells(USCs)have shown great potential for applications in tissue engineering and regenerative *** a growing amount of research on the topic,the effectiveness of USCs in various disease models has been shown and the underlying mechanisms have also been explored,though many aspects still remain *** this review,we aim to provide an up-to-date overview of the biological characteristics of USCs and their applications in skin,bone and articular cartilage *** addition to the identification procedure of USCs,we also summarize current knowledge of the underlying repair mechanisms and application modes of *** concerns and perspectives have also been summarized.

关键词： Urine-derived stem cells Skin Bone Articular cartilage tissue engineering cell therapy

Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing

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BIOcell 2012年第1期36卷 37-45页

作者： RANA IMANI SHAHRIAR HOJJATI EMAMI HOSSEIN FAKHRZADEH NAFISEH BAHEIRAEI ALI M SHARIFI Department of Biomedical engineering Amirkabir University of TechnologyTehranIran Endocrinology and Metabolism Research Institute Tehran University of Medical SciencesTehranIran Razi Institute for Drug research Department of PharmacologySchool of MedicineTehran University of Medical SciencesTehranIran Department of tissue engineering and cell therapy School of advanced sciences in MedicineTehran University of Medical Sciences.TehranIran

The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of *** of cell aggregates is one of the important steps in 3D tissue engineering technology,particularly in organ *** simple methods,hanging drop(HD)and conical tube(CT)were utilized to prepare cell *** size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were *** proliferative ability of the cell aggregates and their ability to spread in culture plates were also *** both methods,the optimum average size of the aggregates was less than 500μ*** aggregates were smaller than HD aggregates.5,000 cells per drop HD aggregates showed a marked ability to attach and spread on the culture *** proliferative ability reduced when the initial cell density was *** these methods,we found that the HD method having better size controlling ability as well as enhanced ability to maintain higher rates of viability,spreading,and *** conclusion,smaller HD aggregates might be a suitable choice as building blocks for making bioink particles in bioprinting technique.

关键词： tissue engineering hanging drop conical tube bioprinting