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细胞流变学研究Ⅰ 肺癌患者红细胞粘弹性特性分析

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中国血液流变学杂志 1993年第1期3卷 1-6页

作者：龙勉陈浩马俐 Kuo-Li Paul Sung 重庆大学生物工程研究中心四川重庆630044 重庆市肿瘤研究所重庆630000 Department of Orthopedics and Institute For Biomedical engineering University of California.

本文采用平面流动腔技术设计了狭缝流动测试系统，用于评价单个细胞的粘弹性特性，从而提供了一种能直接、定量地测定单个细胞变形能力等参数的方法。通过对１５例肺癌患者手术前、后及放疗前红细胞变形性等参数的测量，并同正常对照组... 详细信息

本文采用平面流动腔技术设计了狭缝流动测试系统，用于评价单个细胞的粘弹性特性，从而提供了一种能直接、定量地测定单个细胞变形能力等参数的方法。通过对１５例肺癌患者手术前、后及放疗前红细胞变形性等参数的测量，并同正常对照组比较，验证了该技术和方法。最后，对平面流动腔内的Ｈｅｌｅ—Ｓｈａｗ流动进行了讨论。

关键词：平面流动腔红细胞变形性肺癌

NOx和SOx对电催化还原高炉尾气CO2性能的影响

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河北冶金 2020年第12期 35-39页

作者：徐泽霖张怀东吴宇明李梦然 Sahil Garg Thomas E.Rufford 李立业 Victor Rudolph 王国雄 School of Chemical engineering the University of QueenslandSt Lucia4072BrisbaneQueenslandAustralia 河钢集团邯钢公司河北邯郸O56O15 河钢集团钢研总院河北石家庄050023

如何低成本地降低炼铁过程中的CO2排放是钢铁企业亟待解决的重要课题。电催化还原CO2技术可将CO2转化成可利用的燃料或产品,有望实现低成本降低CO2排放。通过采用含一定浓度的KNO2、KNO3和K2SO3的KHCO3电解液,模拟生产烟气中较高NOx和SO... 详细信息

如何低成本地降低炼铁过程中的CO2排放是钢铁企业亟待解决的重要课题。电催化还原CO2技术可将CO2转化成可利用的燃料或产品,有望实现低成本降低CO2排放。通过采用含一定浓度的KNO2、KNO3和K2SO3的KHCO3电解液,模拟生产烟气中较高NOx和SOx浓度下在电解液中溶解反应生成的产物,研究了其对法拉第效率FECO和FEH2随电催化时间变化的影响。结果表明,KNO2、KNO3和K2SO3都会对电催化还原CO2性能造成不利影响,且相较SOx,虽NOx溶解产物对电催化还原CO2效率的降低影响更大,但可以提高电催化稳定性。下一步应重点研究气相NOx和SOx对催化剂的影响,以及通过催化剂和电极开发来提高反应器对烟气的耐受性。

关键词：流式电解槽电催化还原 NOx SOx 法拉第效率

Differentiation of human mesenchymal stem cell spheroids under microgravity conditions

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cell Regeneration 2012年第1期1卷 2-10页

作者： Wolfgang H Cerwinka Starlette M Sharp Barbara D Boyan Haiyen E Zhau Leland W K Chung Clayton Yates Children’s Healthcare of Atlanta Emory University School of Medicine5445Meridian Mark RoadSuite 420AtlantaGA 30342USA Department of Biology and Center for Cancer Research Tuskegee UniversityCarver Research BuildingTuskegee InstituteAL 36088USA The Wallace H.Coulter Department of Biomedical engineering Georgia Institute of Technology313 Ferst Drive AtlantaAtlantaGA 30332USA Samuel-Oschin Comprehensive Cancer Institute Cedars-Sinai Medical Center8750 Beverly Blvd.Atrium 103Los AngelesCA 90048USA Georgia Pediatric Urology 5445 Meridian Mark RdSuite 420AtlantaGA 30342USA Tuskegee University Carver Research Bld.Rm 22TuskegeeAL 36088USA.

To develop and characterize a novel cell culture method for the generation of undifferentiated and differentiated human mesenchymal stem cell 3D structures,we utilized the RWV system with a gelatin-based scaffold.3×10^(6) cells generated homogeneous spheroids and maximum spheroid loading was accomplished after 3 days of *** cultured in undifferentiated spheroids of 3 and 10 days retained expression of CD44,without expression of differentiation *** cultured in adipogenic and osteogenic differentiation media exhibited oil red O staining and von Kossa staining,*** characterization of osteogenic lineage,showed that 10 day spheroids exhibited stronger calcification than any other experimental group corresponding with significant expression of vitamin D receptor,alkaline phosphatase,and *** conclusion this study describes a novel RWV culture method that allowed efficacious engineering of undifferentiated human mesenchymal stem cell spheroids and rapid osteogenic *** use of gelatin scaffolds holds promise to design implantable stem cell tissue of various sizes and shapes for future regenerative treatment.

关键词： Mesenchymal stem cell RWV culture tissue engineering Differentiation Osteogenesis

复方健耳剂干预小鼠老年性耳蜗感音神经细胞凋亡径路

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中国老年学杂志 2019年第4期39卷 878-882页

作者：陈思仲唐俊波宣伟军韦瑀龙宣毅广西中医药大学第一临床医学院耳鼻咽喉头颈外科广西南宁530023 广西中医药大学瑞康医学院制药厂广西南宁530023 School of engineering Tufts University

目的探讨中药复方健耳剂干预老年性耳蜗感音神经细胞凋亡作用可能径路。方法选择刚断奶C57BL/6J小鼠15只随机分为2月龄对照组、7月龄对照组、7月龄中药组各5只,其中2月龄对照组、7月龄对照组每日饮用自来水直到出生后2、7个月止,7月龄... 详细信息

目的探讨中药复方健耳剂干预老年性耳蜗感音神经细胞凋亡作用可能径路。方法选择刚断奶C57BL/6J小鼠15只随机分为2月龄对照组、7月龄对照组、7月龄中药组各5只,其中2月龄对照组、7月龄对照组每日饮用自来水直到出生后2、7个月止,7月龄中药组每日饮用中药复方健耳剂直到出生后7个月止。各组期满立刻取出耳蜗,利用实时聚合酶链反应技术,检测耳蜗Fas配体(L)、细胞色素(cyt) C基因、含半胱氨酸的天冬氨酸蛋白水解酶(caspase)-3表达量。结果 7月龄对照组耳蜗cyt C、Fas L、caspase-3表达量均显著高于7月龄中药组和2月龄对照组(P<0. 05)。结论 C57BL/6J小鼠老年性耳蜗感音神经细胞凋亡可能均通过线粒体或细胞膜途径介导caspase引起程序性细胞死亡。该中药防治老年性聋关键作用机制之一可能与保护线粒体,防止cyt C外泄或稳定细胞膜,抑制Fas L,以阻止其触发caspase引起细胞程序性死亡径路有关。

关键词：老年性聋中草药细胞凋亡机制

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

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Journal of Innovative Optical Health Sciences 2024年第2期17卷 24-34页

作者： Xiang Zhong Chao Gao Hui Li Yuening He Peng Fei Zaozao Chen Zhongze Gu Dan Zhu Tingting Yu Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China School of Optical and Electronic Infrmation Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China State Key Laboratory of Bioelectronics School of Biological Science and Medical engineering Southeast University NanjingJiangsu 210096P.R.China Institute of Biomaterials and Medical Devices Southeast University Suzhou Jiangsu 215163P.R.China

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal *** modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent *** tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or *** address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and *** our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor *** summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

关键词： tissue optical clearing 3D cell cultures imaging

Effects of theophylline/chitosan/β-cyclodextrin microspheres for oral drug delivery on an asthmatic rat model

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TMR Modern Herbal Medicine 2020年第2期3卷 66-76页

作者： Saisai Wang Haijing Zhang Xiaoming Cui Liping Zhao Mingxia Jiang Zhilu Xu Dongzhen Mu Weifen Zhang School of Pharmacy Weifang Medical UniversityWeifangShandong 261053China Intensive Care Unit The First people’s hospital of HefeiHefeiAnhui 230061China Department of Immunology Key lab for Immunology in Shandong ProvinceSchool of clinical MedicineWeifang Medical UniversityWeifangShandong 261053China Institute for Smart Materials and Regenerative Medicine Weifang Medical UniversityWeifangShandong 261053China Shandong engineering Research Center for Smart Materials and Regenerative Medicine Weifang Medical UniversityWeifangShandong 261053China

Objective:This study aimed to investigate the effects of TH/CTS/β-CD microspheres by oral administration on ovalbumin allergic asthma of a rat ***:Spray drying method was applied for preparing the TH/CTS/β-CD microspheres,which were used in treatment of asthmatic *** a predetermined time,the levels of eosinophil(Eos),protein,lactate dehydrogenase(LDH),aspartate transaminase(AST),glutamic pyruvic transaminase(GPT),and creatinine(Cr)were ascertained by automatic hematology and biochemical *** tissue histology was performed by hematoxylin-eosin ***:No significant differences were found about the contents of Eos in the blood,and the contents of Eos,protein and LDH in the bronchoalveolar lavage fluid between the microsphere-treated and dexamethasone-treated groups,but the levels were lower in both treated groups than in the model *** the microsphere-treated group,the levels of Cr,AST,and GPT in the serum of rats showed no significant differences compared with the normal *** on lung histopathological findings,the microspheres attenuated inflammatory cell infiltration and mucus hypersecretion compared with the model ***:This study demonstrated that TH/CTS/β-CD microspheres exerted an anti-inflammatory effect and could serve as a novel promising drug delivery system for asthma treatment.

关键词： Chitosan Theophylline Asthma Microspheres Pharmacodynamics

复方健耳剂对抗老年性耳蜗神经细胞凋亡超微结构观察及上调NeuN和BDNF定位表达作用

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中国实验动物学报 2020年第3期28卷 338-344页

作者：宣伟军黄力毅宣毅唐俊波韦瑀龙广西中医药大学第一临床医学院耳鼻咽喉科南宁530023 Harvard Medical School Harvard UniversityBostonMA 02114USA School of engineering Tufts UniversityMedfordMA 02155USA 广西中医药大学瑞康医学院制药厂南宁530011

目的利用透射电镜观察中药复方健耳剂干预小鼠老年性耳蜗外毛细胞(outer hair cell,OHC)、螺旋神经节神经元(spiral ganglion neurons,SGN)凋亡作用;共聚焦显微镜,结合形态学多重免疫荧光技术,观察复方健耳剂对耳蜗SGN的特异性核抗原蛋... 详细信息

目的利用透射电镜观察中药复方健耳剂干预小鼠老年性耳蜗外毛细胞(outer hair cell,OHC)、螺旋神经节神经元(spiral ganglion neurons,SGN)凋亡作用;共聚焦显微镜,结合形态学多重免疫荧光技术,观察复方健耳剂对耳蜗SGN的特异性核抗原蛋白(Neu N)、脑源性神经营养因子(BDNF)定位表达影响作用,探讨其作用机制。方法选择1月龄C57BL/6J小鼠22只并随机分为两组,其中11只小鼠每日饮用自来水直到7个月作为老年性耳蜗退变对照组(简称7月龄对照组);11只小鼠每日饮用复方健耳剂1.83 g/(kg·d)直到7个月作为中药干预组(简称7月龄中药组)。所有动物期满取出耳蜗,制备石蜡包埋切片,在透射电镜下,重点选择观察7月龄对照组耳蜗底回存留尚未解体的OHC以及对应部位的SGN超微结构变化,同时与7月龄中药组相同部位的OHC和SGN进行对照。利用激光共聚焦显微镜,结合形态学多重免疫荧光技术,观察Neu N与BDNF在SGN定位表达,并进行统计分析。结果 7月龄对照组耳蜗底回存留尚未解体的OHC以及对应部位的SGN,胞体萎缩,胞核不整,核染色质聚集成团、边集,纹理结构模糊,电子密度高,尤其是SGN大小形态不一,数量明显减少,甚至核染色质溶解,形成空泡,或解体缺如等,呈现严重凋亡状况。而7月龄中药组耳蜗底回OHC或SGN凋亡状况不明显,细胞形态结构较完整,数量较多,核染色质分布较均匀,纹理结构清晰,电子密度低,仅个别胞核溶解,形成空泡。与7月龄对照组比较,7月龄中药组耳蜗底回SGN中的Neu N与BDNF定位表达密度较高,数量较多(P<0.05)。结论耳蜗OHC或SGN细胞核超微结构改变、Neu N表达减少是老年性耳蜗神经细胞凋亡的重要特征;同期SGN凋亡早于OHC;中药复方健耳剂具有明显对抗OHC或SGN超微结构改变,其对SGN中的Neu N表达明显增多,提示中药具有明显保护SGN作用,与透射电镜观察一致,其作用机制与促进BDNF表达,从而发挥系列生物学效应有关。

关键词：小鼠老年性聋耳蜗神经细胞超微结构中医药 NeuN BDNF

A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration

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Bioactive Materials 2024年第5期35卷 99-121页

作者： Ashkan Bigham Ines Fasolino Silvia Borsacchi Carmen Valente Lucia Calucci Gabriele Turacchio Marianna Pannico Manuel Serrano-Ruiz Luigi Ambrosio Maria Grazia Raucci Institute of Polymers Composites and BiomaterialsNational Research Council of Italy(IPCB-CNR)Viale John Fitzgerald Kennedy 54Mostra d’Oltremare Padiglione 2080125NaplesItaly Department of Chemical Materials and Production EngineeringUniversity of Naples Federico IIPiazzale V.Tecchio 8080125NaplesItaly Institute for the Chemistry of OrganoMetallic Compounds-ICCOM Italian National Research Council-CNRvia G.Moruzzi 156124Pis1Italy Center for Instrument Sharing of the University of Pisa(CISUP) 56126Pis1Italy Institute of Experimental Endocrinology and Oncology“G.Salvatore”(IEOS) National Research Council(CNR)Via Pietro Castellino 11180131NapoliItaly Institute of Polymers Compositesand BiomaterialsNational Research Council of Italy(IPCB-CNR)PozzuoliItaly Institute for the Chemistry of OrganoMetallic Compounds-ICCOM National Research Council-CNRSesto FiorentinoItaly Institute of Translational Pharmacology(IFT) National Research Council(CNR)Via Carducci 3267100L’Aquila(Italy).

Recently,the term theragenerative has been proposed for biomaterials capable of inducing therapeutic approaches followed by repairing/regenerating the tissue/*** study is focused on the design of a new theragenerative nanocomposite composed of an amphiphilic non-ionic surfactant(Pluronic F127),bioactive glass(BG),and black phosphorus(BP).The nanocomposite was prepared through a two-step synthetic strategy,including a microwave treatment that turned BP nanosheets(BPNS)into quantum dots(BPQDs)with 5±2 nm dimensions in *** effects of surfactant and microwave treatment were assessed in vitro:the surfactant distributes the ions homogenously throughout the composite and the microwave treatment chemically stabilizes the *** presence of BP enhanced bioactivity and promoted calcium phosphate formation in simulated body *** inherent anticancer activity of BP-containing nanocomposites was tested against osteosarcoma cells in vitro,finding that 150μg mL^(-1)was the lowest concentration which prevented the proliferation of SAOS-2 cells,while the counterpart without BP did not affect the cell growth ***,the apoptosis pathways were evaluated and a mechanism of action was *** irradiation was applied to induce further proliferation suppression on SAOS-2 cells through *** inhibitory effects of bare BP nanomaterials and nanocomposites on the migration and invasion of bone cancer,breast cancer,and prostate cancer cells were assessed in vitro to determine the anticancer potential of nanomaterials against primary and secondary bone *** regenerative behavior of the nanocomposites was tested with healthy osteoblasts and human mesenchymal stem cells;the BPQDs-incorporated nanocomposite significantly promoted the proliferation of osteoblast cells and induced the osteogenic differentiation of stem *** study introduces a new multifunctional theragenerative platform with promising potential for simultaneous bone cancer t

关键词： Bioactive glass Black phosphorus Microwave treatment Bone cancer therapy tissue regeneration

Single-cell transcriptome analysis uncovers underlying mechanisms of acute liver injury induced by tripterygium glycosides tablet in mice

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Journal of Pharmaceutical Analysis 2023年第8期13卷 908-925页

作者： Qiuyan Guo Jiangpeng Wu Qixin Wang Yuwen Huang Lin Chen Jie Gong Maobo Du Guangqing Cheng Tianming Lu Minghong Zhao Yuan Zhao Chong Qiu Fei Xia Junzhe Zhang Jiayun Chen Feng Qiu Jigang Wang State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Centerand Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing100700China School of Chinese Materia Medica and State Key Laboratory of Component-Based Chinese MedicineTianjin University of Traditional Chinese MedicineTianjin301617China Department of Nephrology Shenzhen Key Laboratory of Kidney DiseasesShenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhenGuangdong518020China College of Food Science and engineering Institute of OceanBohai UniversityJinzhouLiaoning121013China State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing100700China The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine ShenzhenGuangdong518033China

Tripterygium glycosides tablet(TGT),the classical commercial drug of Tripterygium wilfordii *** been effectively used in the treatment of rheumatoid arthritis,nephrotic syndrome,leprosy,Behcet's syndrome,leprosy reaction and autoimmune ***,due to its narrow and limited treatment window,TGT-induced organ toxicity(among which liver injury accounts for about 40%of clinical reports)has gained increasing *** present study aimed to clarify the cellular and molecular events underlying TGT-induced acute liver injury using single-cell RNA sequencing(scRNA-seq)*** TGT-induced acute liver injury mouse model was constructed through short-term TGT exposure and further verified by hematoxylin-eosin staining and liver function-related serum indicators,including alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase and total *** the mouse model,we identified 15 specific subtypes of cells in the liver tissue,including endothelial cells,hepatocytes,cholangiocytes,and hepatic stellate *** analysis indicated that TGT caused a significant inflammatory response in liver endothelial cells at different spatial locations;led to marked inflammatory response,apoptosis and fatty acid metabolism dysfunction in hepatocytes;activated hepatic stellate cells;brought about the activation,inflammation,and phagocytosis of liver capsular macrophages cells;resulted in immune dysfunction of liver lymphocytes;disturbed the intercellular crosstalk in liver microenvironment by regulating various signaling ***,these findings elaborate the mechanism underlying TGT-induced acute liver injury,provide new insights into the safe and rational applications in the clinic,and complement the identification of new biomarkers and therapeutic targets for liver protection.

关键词： Tripterygium glycosides tablet Acute liver injury scRNA-seq