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An atlas of immune cell transcriptomes in human immunodeficiency virus-infected immunological non-responders identified marker genes that control viral replication

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Chinese Medical Journal 2023年第22期136卷 2694-2705页

作者： Yahong Chen Xin Li Shuran Liu Wen Ao Jing Lin Zhenting Li Shouli Wu Hanhui Ye Xiao Han Dongliang Li Department of infection Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouFujian 350025China Department of infection Fuzong Clinical Medical College of Fujian Medical UniversityFuzhouFujian 362002China College of Biological Science and engineering Fuzhou UniversityFuzhouFujian 350025China Fujian Provincial Center for Disease Control and Prevention No.76 Jintai RoadFuzhouFujian 350001China Department of Hepatobiliary Medicine 900th Hospital of Joint Logistics Support Forces of the Chinese PLAFuzhouFujian 350000China

Background:Previous studies have examined the bulk transcriptome of peripheral blood immune cells in acquired immunodeficiency syndrome patients experiencing immunological *** study aimed to investigate the characteristics of specific immune cell subtypes in acquired immunodeficiency syndrome patients who exhibit immunological ***:A single-cell transcriptome sequencing of peripheral blood mononuclear cells obtained from both immunological responders(IRs)(CD4^(+)T-cell count>500)and immunological non-responders(INRs)(CD4^(+)T-cell countcell subpopulations,marker genes,and differentially expressed genes aiming to uncover potential genetic factors associated with immunological ***:Among the cellular subpopulations analyzed,the ratios of monocytes,CD16^(+)monocytes,and exhausted B cells demonstrated the most substantial differences between INRs and IRs,with fold changes of 39.79,11.08,and 2.71,*** contrast,the CD4^(+)T cell ratio was significantly decreased(0.39-fold change)in INRs compared with that in ***,the ratios of natural killer cells and terminal effector CD8^(+)T cells were also lower(0.37-fold and 0.27-fold,respectively)in the INRs *** addition to several well-characterized immune cell-specific markers,we identified a set of 181 marker genes that were enriched in biological pathways associated with human immunodeficiency virus(HIV)***,ISG15,IFITM3,PLSCR1,HLA-DQB1,CCL3L1,and DDX5,which have been demonstrated to influence HIV replication through their interaction with viral proteins,emerged as significant monocyte marker ***,the differentially expressed genes in natural killer cells were also enriched in biological pathways associated with HIV ***:We generated an atlas of immune cell transcriptomes in HIV-infected IRs and *** genes associa

关键词： Single-cell transcriptome sequencing Human immunodeficiency virus Immunological non-responsiveness CD4 T cell CD8 T cell Natural killer cells HIV replication

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YBX1 inhibits mitochondrial-mediated apoptosis in ischemic heart through the PI3K/AKT signaling pathway

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Frigid Zone Medicine 2024年第1期4卷 51-64页

作者： Fangfang Bi Miao Cao Yuquan Wang Qingming Pan Zehong Jing Danyang Bing Lifang Lyu Tong Yu Tianyu Li Xuelian Li Haihai Liang Hongli Shan Yuhong Zhou Department of Pharmacology(State-Province Key Laboratories of Biomedicine-Pharmaceutics of China Key Laboratory of Cardiovascular ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbin 150081China Shanghai Frontiers Science Research Center for Druggability of Cardiovascular noncoding RNA Institute for Frontier Medical TechnologyShanghai University of Engineering ScienceShanghai 201620China Department of Basic Medicine Xiamen Medical CollegeXiamen 361023China

Background:Myocardial infarction(MI)is associated with higher morbidity and mortality in the world,especially in cold ***1 is an RNA-binding protein that is required for pathological growth of cardiomyocyte by regulating cell growth and protein *** YBX1,as an individual RNA-binding protein,regulates cardiomyocytes through signaling cascades during myocardial infarction remain largely ***:In vivo,the mouse MI model was induced by ligating the left anterior descending coronary artery(LAD),and randomly divided into sham operation group,MI group,MI+YBX1 knockdown/overexpression group and MI+negative control(NC)*** protective effect of YBX1 was verified by echocardiography and triphenyltetrazolium chloride *** vitro,mitochondrial-dependent apoptosis was investigated by using CCK8,TUNEL staining,reactive oxygen species(ROS)staining and JC-1 staining in hypoxic neonatal mouse cardiomyocytes(NMCMs).Results:YBX1 expression of cardiomyocytes was downregulated in a mouse model and a cellular model on the ischemic *** to mice induced by MI,YBX1 overexpression mediated by adeno-associated virus serotype 9(AAV9)vector reduced the infarcted size and improved cardiac *** of endogenous YBX1 by shRNA partially aggravated ischemia-induced cardiac *** hypoxic cardiomyocytes,YBX1 overexpression decreased lactic dehydrogenase(LDH)release,increased cell viability,and inhibited apoptosis by affecting the expression of apoptosis related proteins,while knockdown of endogenous YBX1 by siRNA had the opposite *** of YBX1 restored mitochondrial dysfunction in hypoxic NMCMs by increasing mitochondrial membrane potential and ATP content and decreasing *** hypoxic NMCMs,YBX1 overexpression increased the expression of phosphorylated phosphatidylinositol 3 kinase(PI3K)/AKT,and the anti-apoptosis effect of YBX1 was eliminated t by LY294002,PI3K/AKT ***:YBX1 protected the heart

关键词： YBX1 PI3K/AKT apoptosis mitochondrial function myocardial infarction

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Exogenous BDNF and Chondroitinase ABC Consisted Biomimetic Microenvironment Regulates Survival,Migration and Differentiation of Human Neural Progenitor cells Transplanted into a Rat Auditory Nerve

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Neuroscience & Medicine 2014年第2期5卷 86-100页

作者： Ajay Kale Ekaterina Novozhilova Ulrica Englund-Johansson Samuel I.Stupp Bjorn Palmgren Petri Olivius Department of Clinical Sciences Intervention and TechnologyKarolinska InstitutetKarolinska University HospitalStockholmSweden Division of Otorhinolaryngology Department of Clinical and Experimental MedicineUniversity of LinkopingLinkopingSweden Department of Ophthalmology Institution of Clinical Sciences in LundLund UniversityLundSweden Department of Chemistry Materials Science and Engineeringand Medicine and Institute for BioNanotechnology in MedicineNorthwestern UniversityChicagoUSA Division of Otorhinolaryngology Linkoping University HospitalLinkopingSweden

Current putative regeneration oriented studies express possible role of stem cell based implantation strategy in the restoration of fundamental perception of hearing. The present work utilizes a rat auditory nerve (AN) directed transplantation of human neural progenitor cells (HNPCs) as a cell replacement therapy for impaired auditory function. groups of b-bungarotoxin induced auditory function compromised female rats were used to transplant HNPCs in the nerve trunk. In the treatment groups, brain derived neurotrophic factor (BDNF), peptide amphiphile nanofiber bioactive gel (Bgel) and Chondroitinase ABC (ChABC), a digestive enzyme that cleaves the core of chondroitin sulphate proteoglycans, were added along with HNPCs while the control groups were with PA inert gel (Igel) and devoid of ChABC. Six weeks post transplantation survival, migration, and differentiation of HNPCs were studied and compared. The groups treated with BDNF and Bgel showed improved survival and differentiation of transplanted HNPCs while the ChABC treated group showed significant migration of HNPCs along the AN and elongation of neuronal fibers along the nerve towards the cochlear nucleus (CN) which was characterized by immunocytochemical markers for human Nuclei (HuN), human mitochondria (HuM) and neuronal β-tubulin (Tuj1). These findings show that addition of BDNF and ChABC consisted Bgel environment facilitated HNPC survival, migration and differentiation along the transplanted rat AN towards the CN. This transplantation strategy provides unique experimental validation for futuristic role of cell based biomaterial consisted neurotrophic factor application in clinically transferable treatment of sensorineural hearing loss (SNHL) along with cochlear implants (CI).

关键词： Auditory Nerve Brain Derived Neurotrophic Factor Chondroitinase Cochlear Nucleus Human Neural Progenitor cells Transplantation Transitional Zone Sensorineural Hearing Loss

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Generation of Microcellular Biodegradable Polycaprolactone Foams in Supercritical Carbon Dioxide

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合成化学 2004年第Z1期12卷 113-113页

作者： Xu Qun Ren Xian-wen Chang Yu-ning Yu Long Wang Jing-wu College of Materials engineering Zhengzhou UniversityZhengzhou 450052P.R.China Manufacturing Science&Technology of Australia

Present now the application of microcellular polymeric materials in biomedical field is growing rapidly, as that of guided tissue regeneration and cell transplantation. As far as guided tissue regeneration is concerned, porous implants are used as size selective membrane to promote the growth of a special tissue in a healing site. Ideally, the implant should be inherently biocompatible,have well-defined cell size and be resorbable with appropriate biodegradation ***(a-caprolactone) (PCL) is a kind of materials suit for the demands above. PCL is biocompatible and biodegradable aliphatic polyester which is nontoxic for living organisms and bioresorbable after a period of implantation. Because of its unique combination of biocompatibility, permeability and biodegradability, PCL and some of its copolymer with lactides and glycolide have been widely applied in medicine as artificial skin, artificial bone and containers for sustained drug *** and Beckman have reported a new method to generate microcellular poly(methy l methacrylate) foams in which the samples are saturated with CO2 under a series of supercritical (SC)conditions, and then the system is rapidly depressurized to atmospheric pressure at constant temperature. Unlike traditional methods, it reduces glass-transition temperature (Tg) of the mixture to below the experimental temperature rather than directly heat the system above Tg. In this process of nucleation, no phase separation occurs as well as no phase boundary meets, so the cellular structure of the foam can be retained *** this work, we have generated PCL foams by using supercritical CO2. Because of the low glass transition temperature (Tg = -60 ℃) of PCL far below the ice point, the experimental temperature in our study is much higher than Tg, which is different from the studies by others before. A series of variable factors on the foam structure as saturation temperature, saturation pressure, saturation time and depressurization ti

关键词： supercritical CO2 biodegradable polycaprolactone microcellular

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The application of ECM-derived biomaterials in cartilage tissue engineering

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Mechanobiology in Medicine 2023年第1期1卷 28-38页

作者： Yu-wei Wang Ming-ze Du Tuo Wu Tong Su Li-ya Ai Dong Jiang Department of Sports Medicine Peking University Third HospitalInstitute of Sports Medicine of Peking UniversityBeijing Key Laboratory of Sports InjuriesEngineering Research Center of Sports Trauma Treatment Technology and DevicesMinistry of EducationBeijingChina School of Basic Medical Sciences Peking UniversityBeijingChina

Given the tremendous increase in the risks of cartilage defects in the sports and aging population,current treatments are limited,and new repair strategies are *** tissue engineering(CTE)is a promising approach to handle this burden and several fabrication technologies and biomaterials have been developed these *** extracellular matrix(ECM)of cartilage consists of a tissue-specific 3D microenvironment with excellent biomechanical and biochemical properties,which regulates cell proliferation,adhesion,migration,and differentiation,thus attracting a great deal of attention to the rapid development of CTE based on ECM *** generations of biomaterials are being developed rapidly for use as scaffolds to mimic the natural ECM *** this review,we discuss such CTE scaffolds based on ECM-derived biomaterials by reviewing the biomaterials for CTE,the applications in different scaffolds and their processing approaches,as well as the current clinical applications of those ECM-based CTE scaffolds.

关键词： Cartilage defects Cartilage tissue engineering Extracellular matrix Biomaterials Repair Clinical applications

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力学环境影响下肿瘤内外血管生成的数值模拟

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医用生物力学 2006年第1期21卷 2-7页

作者：高昊许世雄蔡颖 M.W.Collins 蒋雨平王坚复旦大学力学与工程科学系上海200433 School of engineering Systems and Design South Bank UniversityLondonUK 复旦大学附属华山医院上海200040

目的考察肿瘤内外不同的力学环境对肿瘤血管生成过程中内皮细胞运动的影响,数值生成肿瘤内外血管网络。方法肿瘤外正常组织和肿瘤组织设为线性粘弹性体。肿瘤外正常组织内皮细胞扩散系数是常数;肿瘤内,肿瘤细胞的活性下降导致组织的黏... 详细信息

目的考察肿瘤内外不同的力学环境对肿瘤血管生成过程中内皮细胞运动的影响,数值生成肿瘤内外血管网络。方法肿瘤外正常组织和肿瘤组织设为线性粘弹性体。肿瘤外正常组织内皮细胞扩散系数是常数;肿瘤内,肿瘤细胞的活性下降导致组织的黏附力降低,内皮细胞扩散系数设为离中心的幂次函数。建立内皮细胞运动的扩散方程,数值生成肿瘤内外血管网络。结果当考虑肿瘤内外不同力学环境对内皮细胞运动的影响时,可以获得相对真实的分层毛细血管网络:在肿瘤外层富含毛细血管,越靠近肿瘤中心,血管越稀少。在忽略力学环境的影响时,无法得到实验观测到的分层网络。结论肿瘤内外不同的力学环境对肿瘤血管生成的网络结构有着重要影响。

关键词：肿瘤血管生成力学环境内皮细胞

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Highly sensitive light-up near-infrared fluorescent probe for detection and imaging of β-glucuronidase in human serum,living cells and tumor-bearing mice

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Science China Materials 2022年第3期65卷 836-844页

作者： Xin Zhou Deping Wang Zhiyuan Gao Mingyue He Jiayi Hou Hui Zhang Guoqiang Zhang Dan Ding Guangxue Feng College of Medical Imaging and Key Laboratory of Cellular PhysiologyMinistry of EducationShanxi Medical UniversityTaiyuan 030001China Key Laboratory of Bioactive Materials Ministry of Education College of Life Sciencesand State Key Laboratory of Medicinal Chemical BiologyNankai UniversityTianjin 300071China Department of Clinical Laboratory Shanxi Provincial Academy of Traditional Chinese MedicineTaiyuan 030001China AIE Institute State Key Laboratory of Luminescent Materials and DevicesGuangdong Provincial Key Laboratory of Luminescence from Molecular AggregatesSchool of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510640China

β-Glucuronidase(GUS)plays a key role in tumor initiation,metastasis,and progression,and thus,has been proposed as a promising cancer *** this study,we designed an enzyme-activatable near-infrared(NIR)fluorescent probe(DCM-βGlcA)for the rapid and accurate detection of GUS activity in vitro,in vivo and ex *** DCM-βGlcA was prepared by linking a glucuronic acid residue to dicyanomethylene-4 H-pyran(DCM).This probe exhibited significant light-up NIR fluorescent signals at 680 nm after reacting with GUS and the Stokes shift could reach 150 *** DCM-βGlcA showed a high sensitivity toward GUS and an excellent linear relationship at concentrations ranging between 0 and 4 U L^(-1)(R^(2)=0.9974)with the limit of detection as low as 0.19 U L^(-1).We used the DCM-βGlcA to identify GUS serum levels in both cancer patients and healthy individuals with a similar accuracy as that of an enzyme-linked immunosorbent assay(ELISA)while being easier and faster to ***,the DCM-βGlcA was used for tracking endogenous GUS in living cells,thereby discriminating GUSoverexpressed liver cancer from normal ***,the DCM-βGlcA was able to detect and image endogenous GUS in liver cancer tissue and tumor-bearing mouse *** findings demonstrate the potential of the DCM-βGlcA as a promising tool for detecting and monitoring GUS activity in preclinical applications.

关键词： NIR fluorescent probe β-glucuronidase serum detection cell imaging in-vivo bioimaging

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3D Photo-Fabrication for tissue engineering and Drug Delivery

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engineering 2015年第1期1卷 90-112页

作者： Rúben F.Pereira Paulo J.Bártolo Centre for Rapid and Sustainable Product Development (CDRsp) Polytechnic Institute of Leiria Instituto de Investiga??o e Inova??o em Saúde (I3S) Universidade do Porto Instituto Nacional de Engenharia Biomédica (INEB) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar (ICBAS) Universidade do Porto School of Mechanical Aerospace and Civil EngineeringUniversity of Manchester Manchester Institute of Biotechnology University of Manchester

The most promising strategies in tissue engineering involve the integration of a triad of biomaterials, living cells, and biologically active molecules to engineer synthetic environments that closely mimic the healing milieu present in human tissues, and that stimulate tissue repair and regeneration. To be clinically effective, these environments must replicate, as closely as possible, the main characteristics of the native extracellular matrix(ECM) on a cellular and subcellular scale. Photo-fabrication techniques have already been used to generate 3D environments with precise architectures and heterogeneous composition, through a multi-layer procedure involving the selective photocrosslinking reaction of a light-sensitive prepolymer. cells and therapeutic molecules can be included in the initial hydrogel precursor solution, and processed into 3D constructs. Recently, photofabrication has also been explored to dynamically modulate hydrogel features in real time, providing enhanced control of cell fate and delivery of bioactive compounds. This paper focuses on the use of 3D photo-fabrication techniques to produce advanced constructs for tissue regeneration and drug delivery applications. State-of-the-art photo-fabrication techniques are described, with emphasis on the operating principles and biofabrication strategies to create spatially controlled patterns of cells and bioactive factors. Considering its fast processing, spatiotemporal control, high resolution, and accuracy, photo-fabrication is assuming a critical role in the design of sophisticated 3D constructs. This technology is capable of providing appropriate environments for tissue regeneration, and regulating the spatiotemporal delivery of therapeutics.

关键词： 3D photo-fabrication biomaterials tissueengineering drug delivery

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Unravelling the Functions of Regulatory T cells during Infection

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Open Journal of Medical Microbiology 2018年第4期8卷 118-131页

作者： Tania Rahman Md Ferdous Seraj Annelise Casellato Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Melbourne Australia Department of Microbiology and Immunology University of Melbourne at the Peter Doherty Institute for Infection and Immunity Melbourne Australia School of Civil Environmental and Chemical Engineering RMIT University Melbourne Australia Departamento de Química Inorganica Instituto de Química Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil

Accumulating evidences have suggested that Treg have an active role in the regulation of immunity to infection. Treg suppress not only autoimmune responses but also other immune responses for instance, during acute infections, against commensal microbes in inflammatory diseases or during chronic illness. Treg have been shown to limit exacerbated inflammation to avoid collateral tissue damage. Treg are also suggested to provide early protective responses in some viral infections as the permitting timely entry of effector cells in infected tissue. Furthermore, Treg have been shown to contribute to form memory pool after resolution of infection. In this review, we survey and analysis our current knowledge and relative dynamics of Treg in a wide range of infection settings and elaborate the examples in which these cells are of critical importance in conferring tolerance, suppressing pathogenesis, inducing protection and optimizing immunity to eliminate infection.

关键词： Treg Suppression CD4 T-cell Response CD8 T-cell Response CD25+ Treg RAG-1-Deficient mice Foxp3DTR Mice DEREG Mice Colonic Treg

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沥青摊铺机压实能量模型与最优作业参数研究

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中国工程机械学报 2020年第5期18卷 436-441页

作者：王欣张亮儒李凡卢永军焦生杰长安大学道路施工技术与装备教育部重点实验室陕西西安710064 中国电子科技集团公司第二十研究所导航事业部陕西西安710068 中电海康集团有限公司总工程师办公室浙江杭州310012 合肥京东方显示技术有限公司cell工程部安徽合肥230001

摊铺密实度是衡量沥青混凝土摊铺机作业质量的重要指标之一。施工中实际可获得的摊铺密实度取决于各作业参数的设置及参数间的相互影响。本文从能量等效角度研究了摊铺密实度与作业参数之间的关系,并在此基础上提出了一种最优作业参数... 详细信息

摊铺密实度是衡量沥青混凝土摊铺机作业质量的重要指标之一。施工中实际可获得的摊铺密实度取决于各作业参数的设置及参数间的相互影响。本文从能量等效角度研究了摊铺密实度与作业参数之间的关系,并在此基础上提出了一种最优作业参数的确定方法。首先,通过分析双振捣机构和偏心振动机构对沥青混合料的作用机理,得到了两者在单周期内各自对混合料产生的有效压实能量,结合室内马歇尔击实试验的研究结果,推导了摊铺密实度与摊铺速度、振捣频率、振动频率、振捣冲程和振动振幅等作业参数之间的函数关系;其次,基于简化后的密实度模型,结合摊铺机动力学性能要求对振捣及振动参数取值范围的限制,以及摊铺效率最大化等约束条件,给出了最优作业参数的确定方法;最后,采用本文提出的方法对9 m沥青混凝土摊铺机进行了动力学仿真和计算,针对给定的目标密实度确定了最优作业参数。

关键词：摊铺密实度最优作业参数压实能量沥青混凝土摊铺机

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