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Constructing a biofunctionalized 3D-printed gelatin/sodium alginate/chitosan tri-polymer complex scaffold with improvised biological andmechanical properties for bone-tissue engineering

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Bio-Design and Manufacturing 2024年第1期7卷 57-73页

作者： Amit Kumar Singh Krishna Pramanik Amit Biswas Centre of Excellence in tissue engineering Department of Biotechnology and Medical EngineeringNational Institute of TechnologyRourkela 769008India

Sodium alginate(SA)/chitosan(CH)polyelectrolyte scaffold is a suitable substrate for tissue-engineering *** present study deals with further improvement in the tensile strength and biological properties of this type of scaffold to make it a potential template for bone-tissue *** experimented with adding 0%–15%(volume fraction)gelatin(GE),a protein-based biopolymer known to promote cell adhesion,proliferation,and *** resulting tri-polymer complex was used as bioink to fabricate SA/CH/GEmatrices by three-dimensional(3D)*** studies using scanning electron microscopy revealed the microfibrous porous architecture of all the structures,which had a pore size range of 383–419μm.X-ray diffraction and Fourier-transform infrared spectroscopy analyses revealed the amorphous nature of the scaffold and the strong electrostatic interactions among the functional groups of the polymers,thereby forming polyelectrolyte complexes which were found to improve mechanical properties and structural *** scaffolds exhibited a desirable degradation rate,controlled swelling,and hydrophilic characteristics which are favorable for bone-tissue *** tensile strength improved from(386±15)to(693±15)kPa due to the increased stiffness of SA/CH scaffolds upon addition of *** enhanced protein adsorption and in vitro bioactivity(forming an apatite layer)confirmed the ability of the SA/CH/GE scaffold to offer higher cellular adhesion and a bone-like environment to cells during the process of tissue *** vitro biological evaluation including the MTT assay,confocal microscopy analysis,and alizarin red S assay showed a significant increase in cell attachment,cell viability,and cell proliferation,which further improved biomineralization over the scaffold *** addition,SA/CH containing 15%gelatin designated as SA/CH/GE15 showed superior performance to the other fabricated 3D structures,demonstrating its pote

关键词： Scaffold Biomaterial Sodium alginate Chitosan Gelatin 3D printing tissue engineering

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tissue engineering of blood vessels with endothelial cells differentiated from mouse embryonic stem cells

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Cell Research 2003年第5期13卷 335-341页

作者： GAN SHEN, HSIAO CHIEN TSUNG, CHUN FANG WU, XIAO YIN LIU, XIAOYUN WANG, WEI LIU, LEI CUI, YI LIN CAO Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai 2nd Medical University, Shanghai Key Laboratory of tissue engineering, Shanghai 200011, China Department of Plastic and Reconstructive Surgery Shanghai 9 Peoples Hospital Shanghai 2 Medical University Shanghai Key Laboratory of Tissue Engineering Shanghai China

Endothelial cells （TEC3 cells） derived from mouse embryonic stem （ES） cells were used as seed cells to construct blood vessels. tissue engineered blood vessels were made by seeding 8 × 106 smooth muscle cells （SMCs） obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid （PGA） fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6～8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC3 cells （endothelial cells differentiated from mouse ES cells） were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated th

关键词： tissue engineering embryonic stem cell blood vessel differentiation endothelial cell.

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tissue engineering of cartilage,tendon and bone

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Frontiers of Medicine 2011年第1期5卷 61-69页

作者： Hengyun SUN Wei LIU Guangdong ZHOU Wenjie ZHANG Lei CUI Yilin CAO Department of Plastic and Reconstructive Surgery Shanghai 9th People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of Tissue EngineeringShanghai 200011China National tissue engineering Center of China Shanghai 200241China

tissue engineering aims to produce a functional tissue replacement to repair *** reconstruction is an essential step toward the clinical application of engineered *** progress has recently been achieved in this *** our laboratory,we focus on construction of cartilage,tendon and *** purpose of this review was to summarize the advances in the engineering of these three tissues,particularly focusing on tissue regeneration and defect repair in our *** cartilage engineering,articular cartilage was reconstructed and defects were repaired in animal *** sophisticated tissues,such as cartilage in the ear and trachea,were reconstructed both in vitro and in vivo with specific shapes and *** tendon was generated in vitro and in vivo in many animal models with tenocytes or dermal fibroblasts in combination with appropriate mechanical *** and limb bone defects were also successfully regenerated and repaired in large *** on sophisticated animal studies,several clinical trials of engineered bone have been launched with promising preliminary results,displaying the high potential for clinical application.

关键词： tissue engineering cartilage bone tendon recent advances

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The Effect of Spironolactone Loading on the Properties of 3D-Printed Polycaprolactone/Gold Nanoparticles Composite Scaffolds for Myocardial tissue engineering

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Journal of Bionic engineering 2024年第2期21卷 924-937页

作者： Sharareh Ghaziof Shahrokh Shojaei Mehdi Mehdikhani Mohammad Khodaei Milad Jafari Nodoushan Department of Biomedical engineering Central Tehran BranchIslamic Azad UniversityTehran***Iran Stem Cells Research Center Tissue Engineering and Regenerative Medicine InstituteCentral Tehran BranchIslamic Azad UniversityTehran***Iran Department of Biomedical engineering Faculty of EngineeringUniversity of IsfahanIsfahan***Iran Materials engineering Group Golpayegan College of EngineeringIsfahan University of TechnologyGolpayegan87717-67498Iran Hard tissue engineering Research Center Tissue Engineering and Regenerative Medicine InstituteCentral Tehran BranchIslamic Azad UniversityTehran***Iran

Engineered cardiac constructs(ECC)aid in the progression of regenerative medicine,disease modeling and targeted drug delivery to adjust and aim the release of remedial combination as well as decrease the side effects of *** this research,polycaprolactone/gold nanoparticles(PCL/GNPs)three-dimensional(3D)composite scaffolds were manufactured by 3D printing using the fused deposition modeling(FDM)method and then coated with gelatin/spironolactone(GEL/SPL).Scanning electron microscopy(SEM)and Fourier transform-infrared spectroscopy(FTIR–ATR)were applied to characterize the ***,drug release,biodegradation,behavior of the myoblasts(H9C2)cell line,and cytotoxicity of the 3D scaffolds were *** microstructural observation of the scaffolds reported interconnected pores with 150–300µm in *** 3D scaffolds were degraded significantly after 28 days of immersion in stimulated body fluid(SBF),with the maximum rate of GEL-coated 3D *** release from cross-linked GEL coating demonstrated the excess of drug release over time,and according to the control release systems,the drug delivery systems(DDS)went into balance after the 14th *** addition,cell culture study showed that with the addition of GNPs,the proliferation of(H9C2)was enhanced,and with GEL/SPL coating the cell attachment and viability were improved *** findings suggested that PCL/GNPs 3D scaffolds coated with GEL/SPL can be an appropriate choice for myocardial tissue engineering.

关键词： Polycaprolactone Gold nanoparticles Drug delivery systems Spironolactone Cell behavior Myoblasts

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Histological assessment in peripheral nerve tissue engineering

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Neural Regeneration Research 2014年第18期9卷 1657-1660页

作者： Víctor Carriel Ingrid Garzón Miguel Alaminos Maria Cornelissen Department of Histology (tissue engineering Group) Faculty of MedicineUniversity of Granadaand Instituto de Investigación Biosanitariaibs Department of Basic Medical Sciences (Histology and tissue engineering Group) Faculty of MedicineGhent University

The histological analysis of peripheral nerve regeneration is one of the most used methods to demonstrate the success of the regeneration through nerve conduits. Nowadays, it is possible to evaluate different parameters of nerve regeneration by using histological, histochemical, immunohistochemical and ultrastructural techniques. The histochemical methods are very sensible and are useful tools to evaluate the extracellular matrix remodeling and the myelin sheath, but they are poorly specific. In contrast, the immunohistochemical methods are highly specific and are frequently used for the identification of the regenerated axons, Sehwann cells and proteins associated to nerve regeneration or neural linage. The ultrastructural techniques offer the possibility to perform a high resolution morphological and quantitative analysis of the nerve regeneration. However, the use of a single histological method may not be enough to assess the degree of regeneration, and the combination of different histological techniques could be necessary.

关键词： peripheral nerve regeneration histology, histochemistry immunohistochemistry quantitative histology

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Porosity parameters in biomaterial science: Definition, impact, and challenges in tissue engineering

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Frontiers of Materials Science 2021年第3期15卷 352-373页

作者： Mehdi EBRAHIMI Biomedical and tissue engineering Prince Philip Dental HospitalThe University of Hong KongHong KongChina

Porosity parameters are one of the structural properties of the extracellular microenvironment that have been shown to have a great impact on the cellular phenotype and various biological activities such as diffusion of fluid,initial protein adsorption,permeability,cell penetration and migration,ECM deposition,angiogenesis,and rate and pattern of new tissue *** heterogeneity of the study protocols and research methodologies do not allow reliable meta-analysis for definite *** such,despite the huge available literature,no generally accepted consensus is defined for the porosity requirements of specific tissue engineering ***,based on the biomimetic approach,the biological substitutes should replicate the 3D local microenvironment of the recipient site with matching porosity parameters to best support local cells during tissue ***,the porosity of biomaterials should mimic the porosity of the substituting natural tissue and match the clinical *** analysis of the impact of architectures(i.e.,porosity)on biophysical,biochemical,and biological behaviors will support designing smart biomaterials with customized architectural and functional properties that are patient and defect site-specific.

关键词： porosity pore size pore geometry topography tissue engineering

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Nanoscale viscoelastic properties and adhesion of polydimethylsiloxane for tissue engineering

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Acta Mechanica Sinica 2014年第1期30卷 2-6页

作者： J.Chen K.E.Wright M.A.Birch School of Mechanical & Systems engineering Newcastle University Arthritis Research UK (ARUK) tissue engineering Centre Institute of Cellular MedicineNewcastle UniversityUK Research UK (ARUK) tissue engineering Centre Institute of Cellular MedicineNewcastle UniversityUK St James’ University Hospital University of Leeds UK

It has shown that altering crosslink density of biopolymers will regulate the morphology of Mesenchymal Stem Cells （MSCs） and the subsequent MSCs differentia- tion. These observations have been found in a wide range of biopolymers. However, a recent work published in Nature Materials has revealed that MSCs morphology and differen- tiation was unaffected by crosslink density of polydimethyl- siloxane （PDMS）, which remains elusive. To understand such unusual behaviour, we use nanoindentation tests and modelling to characterize viscoelastic properties and sur- face adhesion of PDMS with different base：crosslink ratio varied from 50：1 （50D） to 10：1 （10D）. It has shown that lower crosslink density leads to lower elastic moduli. De- spite lower nanoindentation elastic moduli, PDMS with lowest crosslink density has higher local surface adhesion which would affect cell-biomaterials interactions. This work suggests that surface adhesion is likely another important physical cue to regulate cell-biomaterials interactions.

关键词： Bioploymer- Nanoindentation- Viscoelasticity Surface adhesion

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tissue engineering of Ligament/tendon-Part II-Importance of Biochemical and Mechanical Factors on the Neogenesis of Ligament

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生物医学工程学杂志 2005年第S1期22卷 27-28页

作者： Sylvaine Muller Shalaw Fawzi-Grancher Said Slimani Research Group of Cell and tissue engineering LEMTA-UMR CNRS 7563 Faculté de Médecine Plateau de Brabois 54500 Vandoeuvre-lès-Nancy FranceResearch Group of Cell and Tissue Engineering LEMTA-UMR CNRS 7563 Faculté de Médecine Plateau de Brabois 54500 Vandoeuvre-lès-Nancy FranceResearch Group of Cell and Tissue Engineering LEMTA-UMR CNRS 7563 Faculté de Médecine Plateau de Brabois 54500 Vandoeuvre-lès-Nancy France

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Regulation challenge of tissue engineering and regenerative medicine in China

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Burns & Trauma 2013年第2期1卷 56-62页

作者： Liang Chen Chunren Wang Tingfei Xi Center for Biomaterials and tissue engineering National Institutes for Food and Drug ControlPeking University Beijing China Center for Biomedical Materials and tissue engineering Peking University Beijing China

The current regulatory status in the USA, European Union (EU), Japan, and China, associated with the clinical application of tissue engineering and regenerative medicine is presented. It is found that similar regulatory framework has been constructed in the USA and EU, in which risk-based regulatory strategy is used to determine which regulatory mode is more desirable between medical technique and medical product. Also, it is suggested that two-tier regulatory framework of medical products be constructed, with the first level built on existing and newly introduced regulatory provisions and the second technical level encompassing all the technical requirements. In China, the demarcation line between medical technique mode and medical product mode is not made clear and coherent and flexible regulatory framework has not been intentionally designed. If the recommendations concerning the desirability of regulatory framework will be adopted in China, it means the present application of medical technique mode should be reconsidered and adjusted based on the risk analysis. Furthermore, the construction of two-tier regulatory framework which is tailored to meet the demands of development of medical products of tissue engineering and regenerative medicine still remains a challenge.

关键词： Regenerative medicine tissue engineering medical product regulation technique

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Current progress of skin tissue engineering:Seed cells, bioscaffolds, and construction strategies

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Burns & Trauma 2013年第2期1卷 63-72页

作者： Huanjing Bi Yan Jin Xi'an Institute of tissue engineering and Regenerative Medicine Fourth Military Medical University Xi'an Shaanxi China Xi'an Institute of tissue engineering and Regenerative Medicine Fourth Military Medical University Xi'an Shaanxi China Research and Development Center for tissue engineering College of Stomatology Fourth Military Medical University Xi'an Shaanxi China

The development of cell biology, molecular biology, and material science, has been propelling biomimic tissue-engineered skins to become more sophisticated in scientificity and more simplified in practicality. In order to improve the safety, durability, elasticity, biocompatibility, and clinical efficacy of tissue-engineered skin, several powerful seed cells have already found their application in wound repair, and a variety of bioactive scaffolds have been discovered to influence cell fate in epidermogenesis. These exuberant interests provide insights into advanced construction strategies for complex skin mimics. Based on these exciting developments, a complete full-thickness tissue-engineered skin is likely to be generated.

关键词： Regenerative medicine wound healing biomaterials seed cells tissue-engineered skin

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