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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第2期8卷 98-104页

作者： ZHANG Jian GAO Nian-fa tianjin key lab of lndustrial microbiology College of Biotechnology Tianjin University of Science and Technology Tianjin 300457 China

Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.

关键词： Response surface methodology Torulopsis glabrata Pyruvic acid Fermentation Medium optimization

Chloride channel-dependent copper acquisition of laccase in the basidiomycetous fungus Cryptococcus neoformans

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Science China(Life Sciences) 2010年第1期53卷 125-130页

作者： ZHU ChangWei1,JIANG Nan1,XIAO DongGuang2,PAN Jiao1 & ZHU XuDong1 1 State key Program of microbiology and Department of microbiology,College of Life Sciences,Nankai University,tianjin 300071,China 2 tianjin Industrial microbiology key lab,College of Bioengineering,tianjin University of Science & Technology,tianjin 300457,China 1. State key Program of microbiology and Department of microbiology College of Life Sciences Nankai University Tianjin 300071 China2. Tianjin Industrial Microbiology Key Lab College of Bioengineering Tianjin University of Science & Technology Tianjin 300457 China

The CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated *** study reports that copper homeostasis was impaired in the clc-a *** was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption ***-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in ***.

关键词： chloride channel laccase copper homeostasis Cryptococcus neoformans

Control of epileptiform spikes based on nonlinear unscented Kalman filter

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Chinese Physics B 2014年第1期23卷 41-48页

作者：刘仙高庆李小俚 key lab of lndustrial Computer Control Engineering of Hebei Province Institute of Electrical Engineering Yanshan University Qinhuangdao 066004 China

A new control strategy based on nonlinear unscented Kalman filter(UKF) is proposed for a neural mass model that serves as a model for simulating real epileptiform stereo-electroencephalographic (SEEG) signals. The UKF is used as an observer to estimate the state from the noisy measurement because it has been proved to be effective for state estimation of nonlinear systems. A UKF controller is constructed via the estimated state and is illustrated to be effective for epileptiform spikes suppression of aforementioned model by numerical simulations.

关键词： unscented Kalman filter(UKF) control epileptiform spike closed-loop control neural mass model

Using transcriptomics to reveal the molecular mechanism of higher alcohol metabolism in Saccharomyces cerevisiae

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Food Bioscience 2023年第1期51卷 210-219页

作者： Danyao Cui Ling Liu Xiaoyuan Zhang Liangcai Lin Xin Li Ting Cheng Chunhui Wei Yu Zhang Zheng Zhou Wei Li Cuiying Zhang key laboratory of Industrial Fermentation microbiology Ministry of EducationTianjin Key Laboratory of Industrial MicrobiologyCollege of BiotechnologyTianjin University of Science and TechnologyTianjin300457PR China Shandong Acad Pharmaceut Sci Natl Local Joint Engn Lab Polysaccharide DrugsKey Lab Biopharmaceut Engn Lab PolysaccharidJinan250101PR China Liquor Making Biological Technology and Application of key laboratory of Sichuan Province Sichuan643002PR China

High alcohols are important flavor compounds in alcoholic beverages,but high concentration of high alcohols is harmful to human *** alcohol synthesis pathways in brewing microorganisms have been investigated,but the interactions between key genes remain to be explored,especially in industrial strains of Saccharomyces *** PDC1 gene was considered to be the main encoding gene ofα-keto acid decarboxylase,and its deletion resulted in a 92.23%increase in isobutanol production and a 14.89%decrease in isoamyl alcohol *** sequencing was used to explore the effects of PDC1 gene deletion on global gene transcription levels,and deletion strategies were utilized to verify the effects of differential genes on higher alcohol *** of differential gene HMLALPHA2 increased isobutanol production by 26.23%,and decreased isoamyl alcohol and 2-phenylethanol production by 30.31%and 22.35%,*** THI2,THI4,THI20 genes were proved to be related to n-propanol *** addition,HMRA2 and SIR3 genes were found to influence isoamyl alcohol synthesis pathways,and their deletion increased isoamyl alcohol production by 25.18%and 21.76%.Our discovery of new target genes is useful for elucidating the molecular mechanisms of higher alcohols and the construction of novel low-producing higher alcohol strains.

关键词： Saccharomyces cerevisiae Higher alcohol Transcriptomics sequencing Alcoholic beverage α-keto acid decarboxylase PDC1 gene

SCO3129,a TetR family regulator,is responsible for osmotic stress in Streptomyces coelicolor

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Synthetic and Systems Biotechnology 2018年第4期3卷 261-267页

作者： Xihong He Hong Li Yuanyuan Pan Linqi Wang Huarong Tan Gang Liu State key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China key lab of Industrial Fermentation microbiology(tianjin University of Science and Technology) Ministry of EducationTianjin300457China State key laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100049China

Streptomyces are the soil-dwelling bacteria with a complex lifecycle and a considerable ability to produce a variety of secondary *** is important for their lifecycle in *** the genome of Streptomyces coelicolor M145,SCO3128(encodes a putative fatty acid desaturase),SCO3129(encodes a putative TetR family regulator)and SCO3130(encodes a putative L-carnitine dehydratase)constitute a transcriptional unit,and its transcript was found to be in response to osmotic *** of SCO3130 led to a bald phenotype on MMG medium and the mycelia lysis on the edge of the colony when KCl/NaCl was added to the *** results indicated that SCO3130 is important for the osmotic stress resistance in *** analysis and electrophoretic mobility shift assays(EMSA)demonstrated that SCO3129 repressed the transcription of SCO3128-3130 operon through directly binding to the promoter region of SCO3128,indicating that SCO3129 regulates the transcription of SCO3128-3130 in response to osmotic stress.

关键词： Osmotic stress Regulation Streptomyces coelicolor

SCKF-STF-CN:a universal nonlinear filter for maneuver target tracking

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Journal of Zhejiang University-Science C(Computers and Electronics) 2011年第8期12卷 678-686页

作者： Quan-bo GE Wen-bin LI Cheng-lin WEN Institute of Information and Control Hangzhou Dianzi University Hangzhou 310018 China State key lab of lndustrial Control Technology Zhejiang University Hangzhou 310027 China

Square-root cubature Kalman filter (SCKF) is more effective for nonlinear state estimation than an unscented Kalman *** this paper,we study the design of nonlinear filters based on SCKF for the system with one step noise correlation and abrupt state ***,we give the SCKF that deals with the one step correlation between process and measurement noises,SCKF-CN in ***,we introduce the idea of a strong tracking filter to construct the adaptive square-root factor of the prediction error covariance with a fading factor,which makes SCKF-CN obtain outstanding tracking performance to the system with target maneuver or abrupt state ***,the tracking performance of SCKF is greatly improved.A universal nonlinear estimator is proposed,which can not only deal with the conventional nonlinear filter problem with high dimensionality and correlated noises,but also achieve an excellent strong tracking performance towards the abrupt change of target *** simulation examples with a bearings-only tracking system are illustrated to verify the efficiency of the proposed algorithms.

关键词： Nonlinear system Maneuver target tracking Correlated noises Square-root cubature Kalman filter (SCKF) Strong tracking filtering (STF)

Rhizoma paridis saponins protected against liver injury in diethylnitrosamine-induced mice

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Traditional Medicine Research 2023年第2期8卷 7-12页

作者： Chen Luo Jing-Wen Yao Shu-Li Man Wen-Yuan Gao key laboratory of Industrial microbiology Ministry of Education/Tianjin Key Laboratory of Industry Microbiology/National and Local United Engineering Lab of Metabolic Control Fermentation Technology/College of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China tianjin key laboratory for Modern Drug Delivery and High Efficiency School of Pharmaceutical Science and TechnologyTianjin UniversityTianjin 300072China

Background:Diethylnitrosamine,one of food additives,possessed a strong carcinogenic effect in *** paridis saponins,as the main active components of Paris polyphylla,have a good anti-cancer effect in our previous *** verify their inhibitory effect on diethylnitrosamine-induced liver cancer,we carried out this ***:We established diethylnitrosamine-induced mouse hepatocarcinoma models to evaluate antitumor of Rhizoma paridis ***,gas chromatography-mass spectrometry was applied to analyze the metabolites in the urine and serum ***:Rhizoma paridis saponins alleviated diethylnitrosamine-induced *** the one hand,Rhizoma paridis saponins down-regulated the levels of liver function markers,such as alanine aminotransferase,aspartate transaminase and alpha *** the other hand,Rhizoma paridis saponins reduced metabolic disorders by increasing fructose and mannose metabolism,and decreasing pentose and glucuronate interconversion,inositol phosphate metabolism,and the process of saturated fatty acids transforming to unsaturated fatty acids,which based on the regulating mRNA expression of glucose transporter type 4,lactate dehydrogenase A,fatty acid synthetas,acetyl-CoA carboxylase and apolipoprotein ***:Rhizoma paridis saponins has the potential application to inhibit chemical-induced hepatocarcinogenesis in the future.

关键词： Rhizoma paridis saponins liver injury diethylnitrosamine metabolites

Systems metabolic engineering strategies for the production of amino acids

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Synthetic and Systems Biotechnology 2017年第2期2卷 87-96页

作者： Qian Ma Quanwei Zhang Qingyang Xu Chenglin Zhang Yanjun Li Xiaoguang Fan Xixian Xie Ning Chen National and Local United Engineering lab of Metabolic Control Fermentation Technology Tianjin University of Science and TechnologyTianjin300457China key laboratory of Industrial Fermentation microbiology Ministry of EducationTianjin University of Science and TechnologyTianjin300457China College of Biotechnology Tianjin University of Science and TechnologyTianjin300457China

Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary *** is an efficient approach for strain improvement and process optimization,and has been successfully applied in the microbial production of various chemicals including amino *** this review,systems metabolic engineering strategies including pathwayfocused approaches,systems biology-based approaches,evolutionary approaches and their applications in two major amino acid producing microorganisms:Corynebacterium glutamicum and Escherichia coli,are summarized.

关键词： Systems metabolic engineering Amino acid Corynebacterium glutamicum Escherichia coli

Gut liver brain axis in diseases:the implications for therapeutic interventions

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Signal Transduction and Targeted Therapy 2024年第1期9卷 79-104页

作者： Mengyao Yan Shuli Man Benyue Sun Long Ma Lanping Guo Luqi Huang Wenyuan Gao State key laboratory of Food Nutrition and Safety Key Laboratory of Industrial MicrobiologyMinistry of EducationTianjin Key Laboratory of Industry MicrobiologyNational and Local United Engineering Lab of Metabolic Control Fermentation TechnologyChina International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal ChemistryCollege of BiotechnologyTianjin University of Science&Technology300457 TianjinChina National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences100700 BeijingChina tianjin key laboratory for Modern Drug Delivery&High-Efficiency School of Pharmaceutical Science and TechnologyTianjin UniversityWeijin Road300072 TianjinChina

Gut-liver-brain axis is a three-way highway of information interaction system among the gastrointestinal tract,liver,and nervous *** the past few decades,breakthrough progress has been made in the gut liver brain axis,mainly through understanding its formation mechanism and increasing treatment *** this review,we discuss various complex networks including barrier permeability,gut hormones,gut microbial metabolites,vagus nerve,neurotransmitters,immunity,brain toxic metabolites,β-amyloid(Aβ)metabolism,and epigenetic regulation in the gut-liver-brain *** therapies containing antibiotics,probiotics,prebiotics,synbiotics,fecal microbiota transplantation(FMT),polyphenols,low FODMAP diet and nanotechnology application regulate the gut liver brain ***,some special treatments targeting gut-liver axis include farnesoid X receptor(FXR)agonists,takeda G protein-coupled receptor 5(TGR5)agonists,glucagon-like peptide-1(GLP-1)receptor antagonists and fibroblast growth factor 19(FGF19)*** gut-brain axis embraces cognitive behavioral therapy(CBT),antidepressants and tryptophan metabolism-related *** liver-brain axis contains epigenetic regulation and Aβmetabolism-related *** the future,a better understanding of gut-liver-brain axis interactions will promote the development of novel preventative strategies and the discovery of precise therapeutic targets in multiple diseases.

关键词： metabolism therapeutic immunity

Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type E3 ligase during legume nodulation

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Molecular Plant 2024年第7期17卷 1090-1109页

作者： Hao Li Yajuan Ou Jidan Zhang Kui Huang Ping Wu Xiaoli Guo Hui Zhu Yangrong Cao National key lab of Agricultural microbiology Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China National key lab of Agricultural microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China

The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands;however,such mechanisms regulating nodulation factor(NF)receptor(NFR)-mediated perception of NFs to establish symbiosis remain *** this study,we unveil the pivotal role of the NFR-interacting RING-type E3 ligase 1(NIRE1)in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus *** demonstrated that NiRE1 has a dual function in this regulatory *** associates with both NFR1 and NFR5,facilitating their degradation through K48-linked polyubiquitination before rhizobial ***,following rhizobial inoculation,NFR1 phosphorylates NIRE1ata conserved residue,Tyr-109,inducing a functional switch in NIRE1,which enables NIRE1tomediateK63-linkedpolyubiquitination,thereby stabilizing NFR1/NFR5 in infected root *** introduction of phospho-dead NIRE1Y1osF leads to delayed nodule development,underscoring the significance of phosphorylation at Tyr-1o9 in orchestrating symbiotic ***,expression of the phospho-mimic NIRE1Y0E results in the formation of spontaneous nodules in ***,further emphasizing the critical role of the phosphorylation-dependent functional switch in *** summary,these findings uncover a fine-tuned symbiotic mechanism that a single E3 ligase could undergo a phosphorylationdependent functional switch to dynamically and precisely regulate NF receptor protein levels.

关键词： nodule symbiosis nodulation factor receptors E3 ligase ubiquitination phosphorylation protein degradation protein stabilization Lotus japonicus